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Ultraviolet - Visible
Spectroscopy (UV)
UV
Background Theory
The larger the gap between the energy levels, the greater
the energy required to promote the electron to the higher
energy level; resulting in light of higher frequency,
and therefore shorter wavelength, being absorbed.
INTRODUCTION 2
...it appears
as this colour
Yellow
570 nm
Red
Orange
Yellow
Green
Blue
Violet
UV Region
190 nm
If a substance
absorbs here...
400 nm
400 nm
450 nm
590 nm
INTRODUCTION 3
620-750 nm
590-620 nm
570-590 nm
496-570 nm
450-495 nm
380-450 nm
Violet
Blue
495 nm
Red
620 nm
Orange
Green
...it appears
as this colour
Yellow
590 nm
570 nm
Red
620-750 nm
UV-Visible Spectrometer
Orange
Yellow
Green
Blue
Violet
590-620 nm
570-590 nm
496-570 nm
450-495 nm
380-450 nm
SOURCE
DETECTORS
REFERENCE
CELL
MONOCHROMATOR
PRISM
BEAM
SPLITTER
RATIO
SAMPLE
CELL
INTRODUCTION 4
UV-Visible Spectrum
1.0
max=217nm
absorbance
0.8
0.6
0.4
0
220
240
260
280
A = ecl
Where
A
= absorbance
0.2
200
300
wavelength (nm)
absorbance
The spectrum for the blue copper complex shows that the
complementary yellow light is absorbed.
350
400
450
500
550
wavelength (nm)
600
650
700
INTRODUCTION 5