Documente Academic
Documente Profesional
Documente Cultură
2015
International Journal of Biosciences | IJB |
ISSN: 2220-6655 (Print), 2222-5234 (Online)
http://www.innspub.net
Vol. 6, No. 5, p. 209-219, 2015
RESEARCH PAPER
OPEN ACCESS
rufipogon Griff.)
Hamid Ali 1* , Fida Muhammad Abbasi 1 , Habib Ahmad 1, Muhammad Abid Khan 2 ,
Hidayat Ullah1 , Syyed Gauhar Jamal 1 , Abdul Waheed 1 , Shujaul Mulk Khan 2,
Muniba Fida Abbasi 1
1
Key words: Oryza sativa, Oryza rufipogon, Wide Hybridization, Cross ability.
http://dx.doi.org/10.12692/ijb/6.5.209-219
Abstract
Oryza rufipogon Griff., is a valuable source of resistant genes to various biotic and abiotic stresses. These
resistant genes can be easily transferred to cultivated rice (Oryza sativa L.), through wide hybridization.
Therefore, the present study was carried out to evaluate interspecific crosses between O. sativa cv. Bas-385, IR6, KS-282 and O. rufipogon (Acc. 106516). Immature spikelets of cultivated varieties () were emasculated and
shed with sufficient pollens of O. rufipogon (). The pollinated spikelets were sprayed immediately with 75 ppm
GA3 to enhance pollen germination. A mixture of 100 ppm GA3 and 25 ppm NAA was then applied twice daily for
5 consecutive days to prevent embryo abortion. The cross seeds (F0) were harvested 30 days after pollination.
Seed setting rate and cross ability was determined for each cross combination. The cross ability between O.
sativa and O. rufipogon varied from 4.35-8.33 (%). The highest cross ability was observed in IR-6 O.
rufipogon (8.33%), followed by Bas-385 O. rufipogon (6.39%) and KS-282 O. rufipogon (4.35%). The hybrid
plants were intermediate between the two parents in phenotypes such as basal leaf sheath color but there was an
overall dominance of wild traits in the F1 hybrids. In order to assess meiotic affinity between O. sativa and O.
rufipogon, chromosome behaviors in pollen mother cells (PMCs) of parents and their F 1 hybrids were analyzed.
The frequencies of abnormalities such as univalents (I), trivalents (III) and laggards were slightly higher in all
hybrids at metaphase-I. Despite the low occurrence of such abnormalities, on the average all hybrids showed
normal meiosis with remarkably, high degree of chromosome pairing. At metaphase-I, all hybrids had more than
10.96 bivalents and 21.78 chiasma/PMC. Thus favorable alleles can be easily transferred from O. rufipogon to
cultivated rice.
* Corresponding
Int. J. Biosci.
2015
Introduction
sativa,
2003;
chromosome
manipulation
are
the
important
which
Londo
is
et
further
al.,
subdivided
2006).
It
is
into
two
perennial,
as
(MAALs),
genomic
chromosomal
monosomic
alien
changes,
addition
including
lines
Because of their
Plant material
International
Philippines.
Rice
Research
Institute
(IRRI),
Int. J. Biosci.
2015
bivalents
diakinesis/metaphase-I
or
other
configuration
and
distribution
at
of
L-1,
Results
Production and confirmation of interspecific crosses
pollinated
afternoon
following
pollination.
The
Table 1. Seed setting rate and cross ability of interspecific crosses of O. sativa and O. rufipogon.
Cross
combination
IR-6
O. 624
19.7
42.28
52
8.33
O. 460
17.3
25.12
20
4.35
O. 360
16.3
39.20
23
6.39
rufipogon
KS-282
rufipogon
Bas-385
rufipogon
Int. J. Biosci.
2015
their
morphological
ancestral
wild
species.
markers,
Among
the
the
anthocyanin
Table 2. Meiotic configuration of IR-6, O. rufipogon (Acc. 106516) and their F1 hybrids.
Chromosome pairing
IR-6
O. rufipogon
IR-6 x O. rufipogon
DK
M1
DK
M1
DK
M1
42
26
94
52
46
100
Range
02
02
02
02
08
04
Mean/cell
0.10
0.08
0.21
0.14
1.61
0.37
Range
11 12
11 12
11 12
10 12
8 12
8 12
Mean/cell
11.95
11.96
11.75
11.90
11.20
11.62
Range
10 12
11 12
10 12
10 12
8 12
5 12
Mean/cell
11.88
11.96
11.69
11.64
10.83
11.02
Range
02
02
02
04
06
Mean/cell
0.07
0.07
0.27
0.37
0.60
Range
01
01
Mean/cell
0.02
0.05
Range
02
Mean/cell
0.06
Range
22 24
22 24
22 24
22 24
16 24
16 24
Mean/cell
23.86
23.92
23.46
23.58
21.50
22.98
Cell analyzed(no)
Univalents
Total Bivalent
Rings
Rods
Trivalent
Quadrivalents
Chiasma/ PMC
DK=Diakinesis
M1=Metaphase-I
Cytogenetic
rufipogon
characterization
and
their
of
O.
F1
sativa,
O.
hybrids
Int. J. Biosci.
2015
Table 3. Meiotic configuration of KS-282, O. rufipogon (Acc. 106516) and their F1 hybrids.
Chromosome pairing
KS-282
O. rufipogon
KS-282 x O. rufipogon
DK
M1
DK
M1
DK
M1
53
33
94
52
109
49
Range
02
02
02
02
0 12
0 22
Mean/cell
0.15
0.18
0.21
0.14
0.92
1.20
Range
11 12
11 12
11 12
10 12
6 12
1 12
Mean/cell
11.92
11.91
11.75
11.90
10.36
10.96
Range
9 12
10 12
10 12
10 12
5 12
1 12
Mean/cell
11.81
11.82
11.69
11.64
9.68
9.96
Range
03
02
02
02
06
04
Mean/cell
0.11
0.09
0.07
0.27
0.67
1.00
Range
01
02
01
Mean/cell
0.02
0.06
0.02
Range
01
02
Mean/cell
0.02
0.20
Range
21 24
22 24
22 24
22 24
12 24
2 24
Mean/cell
23.74
23.73
23.46
23.58
20.21
21.78
Cell analyzed(no)
Univalents
Total Bivalent
Rings
Rods
Trivalent
Quadrivalents
Chiasma/ PMC
at metaphase-1.
Int. J. Biosci.
2015
Bas-385
O. rufipogon
Bas-385x O. rufipogon
DK
M1
DK
M1
DK
M1
100
39
94
52
57
36
04
0.14
02
0.05
02
0.21
02
0.14
08
1.04
04
0.47
10 12
11.93
11 12
11.97
11 12
11.75
10 12
11.90
8 12
11.40
10 12
11.69
10 12
11.89
11 12
11.87
10 12
11.69
10 12
11.64
8 12
10.95
9 12
11.44
01
0.04
01
0.10
02
0.07
02
0.27
02
0.46
03
0.25
_
_
_
_
_
_
01
0.02
01
0.02
01
0.03
_
_
_
_
_
_
_
_
0 10
0.02
01
0.03
20 24
23.82
22 24
23.85
22 24
23.46
22 24
23.58
16 24
22.44
20 24
23.33
Univalents
Range
Mean/cell
Total Bivalent
Range
Mean/cell
Rings
Range
Mean/cell
Rods
Range
Mean/cell
Trivalent
Range
Mean/cell
Quadrivalents
Range
Mean/cell
Chiasma/PMC
Range
Mean/cell
Int. J. Biosci.
2015
3).
Table 5. Chromosome behavior at anaphase-I and telophase-I in parents and their hybrids.
Parent/hybrids
Laggards
Bridges
Bridges + Fragments
Abnormal spindle
Late disjunction
O. rufipogon
120
96.6
1.7
1.7
IR-6
186
97.85
2.15
KS-282
198
98.98
1.01
BAS-385
103
98.06
1.94
IR-6x O.rufipogon
136
87.51
4.41
1.47
2.94
2.2
KS-282x O.rufipogon
190
78.90
8.42
0.53
1.05
6.32
3.2
Bas-385x O.rufipogon
175
86.26
5.14
0.57
3.43
4.6
Discussion
embryo abortion.
Bas-385.
Int. J. Biosci.
2015
(PMCs)
similar
such
observations
were
reported
in
several
as
of
parents
univalents
and
(I),
their
hybrids
trivalents
(III)
were
and
chiasma/PMC.
Fig 3. Meiotic behavior of interspecific hybrid (O. sativa cv. Bas-385 O. rufipogon). (a) diakinesis showing 12
bivalents (11 rings + 1 rod), (b) Metaphase-I showing two straggling bivalents (arrow), (c) Cytokinesis with
abnormal spindle orientations (arrow), (d) Tetrad with abnormal nuclei (arrows).
Meiotic behavior in this study was regular in all the
various
degrees.
However
in
this
study,
the
Int. J. Biosci.
2015
Univalents
and
frequentlyobserved
hybrids,
but
also
quadrivalents
not
only
commonly
in
were
interspecific
reported
for
Conclusions
Common wild rice O. rufipogon produced viable
pairing
at
metaphase-I.
alleles
of
resistant
O.
sativa
O.
rufipogon
hybrids
earlier
Therefore,
genes
can
favorable
be
easily
rice.
Acknowledgements
We
highly
acknowledge
Higher
Education
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