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14 May 2009
doi:10.1016/j.jdermsci.2009.07.007
LSC
Normal controls
Total No.
Male
Female
Age range (year)
Average age (year)
51
36
15
1562
33.5 10.3
24
15
9
1971
35.9 14.2
24
13
11
1952
32.3 9.3
Biopsy sites
Torso
Neck
Extremities
28
0
23
0
14
10
6
7
11
133
Table 2
Total length of nerve bers and neural-LC contacts in lesions and non-lesional skin
of patients with psoriasis vulgaris (PV) and lichen simplex chronicus (LSC).
Biopsy specimen
No. of cases
Total
length (mm)
Nerual-LC
contacts (%)
Normal Controls
24
206.6 64.2
22.5 5.9
PV
Non-lesional skin
Lesions
51
51
209.3 60.4
325.1 85.3**
23.2 6.2
50.1 4.5**
LSC
Non-lesional skin
Lesions
24
24
204.8 56.2
337.3 91.9**
24.4 7.7
57.2 8.1**
**
P < 0.01; non-lesional skin is the normal-appearing skin of the opposite side of
the same patient.
Table 3
Real-time PCR results of NGF mRNA expression in lesions and non-lesional skin of patients with psoriasis vulgaris (PV) and lichen simplex chronicus (LSC).
b-Actin
Normal controls
Non-lesional skin of PV
PV lesions
Non-lesional skin of LSC
LSC lesions
NGF
Quantitative value
Relative expression
quantity
Quantitative value
Corrected
quantitative value
257.0 18.5
282.6 21.3
362.5 30.6
313.0 55.3
382.5 23.4
1.0
1.1
1.4
1.2
1.5
1123.5 362.1
1614.2 358.8
2396.7 572.0
1858.9 396.2
2796.9 576.4
1123.5 362.1
1345.2 299.0
1711.9 408.6
1549.1 330.2
1864.6 378.3
t value
5.17
6.02
3.07
6.93
Relative expression
quantity
1.0
1.2
1.5
1.4
1.7
Note: t value refers to the statistical signicance between PV or LSC lesion groups and non-lesional skin of PV or LSC groups, respectively, and the statistical signicance
between PV or LSC lesion groups and normal controls, respectively. All the P value are less than 0.01.
Fig. 1. Representative images of IHC staining of nerve bers (200). Nerve bers are shown in dark brown color and pointed out by arrows. (a) The lesion of a patient with LSC.
(b) The normal-appearing skin of the same patient with LSC. (c) The skin from a normal control. (d) The lesion of a patient with PV. (e) The normal-appearing skin of the same
patient with PV.
134
Fig. 2. Representative uorescent images of contacts between LCs and nerve bers (200). LCs are in bright green color (upper traces); nerve bers are in red color (middle
traces); the overlapping contacts of LCs and nerve bers are in yellow color (lower traces). (a) 13: the lesion of a patient with LSC. (b) 13: the normal-appearing skin of the
same patient with LSC. (c) 13: the skin from a normal control. (d) 13: the lesion of a patient with PV. (e) 13: the normal-appearing skin of the same patient with PV.
Shaoshan Cuia,1
Ting Xiaoa,1
Yan Wanga
Hongguang Lua
Yakun Wanga
Xing-Hua Gaoa
Huachen Weib
Hong-Duo Chena,*
a
Department of Dermatology, No.1 Hospital of China Medical
University, 155 North Nanjing Street, Shenyang 110001, China
b
Department of Dermatology, Mount Sinai School of Medicine,
New York, NY 10013, USA
*Corresponding author. Tel.: +86 24 83282642;
fax: +86 24 83282633
E-mail address: chenhd@cae.cn (Hong-Duo Chen)
1
Both authors contributed equally to this paper.
5 March 2009
doi:10.1016/j.jdermsci.2009.07.009