Documente Academic
Documente Profesional
Documente Cultură
doi: 10.1111/j.1469-185X.2010.00134.x
33
ABSTRACT
The extraordinary taxonomic and morphological diversity of orchids is accompanied by a remarkable range of
pollinators and pollination systems. Sexually deceptive orchids are adapted to attract specific male insects that are fooled
into attempting to mate with orchid flowers and inadvertently acting as pollinators. This review summarises current
knowledge, explores new hypotheses in the literature, and introduces some new approaches to understanding sexual
deception from the perspective of the duped pollinator. Four main topics are addressed: (1) global patterns in sexual
deception, (2) pollinator identities, mating systems and behaviours, (3) pollinator perception of orchid deceptive signals,
and (4) the evolutionary implications of pollinator responses to orchid deception, including potential costs imposed on
pollinators by orchids. A global list of known and putative sexually deceptive orchids and their pollinators is provided
and methods for incorporating pollinator perspectives into sexual deception research are provided and reviewed.
At present, almost all known sexually deceptive orchid taxa are from Australia or Europe. A few sexually deceptive
species and genera are reported for New Zealand and South Africa. In Central and Southern America, Asia, and the
Pacific many more species are likely to be identified in the future. Despite the great diversity of sexually deceptive orchid
genera in Australia, pollination rates reported in the literature are similar between Australian and European species.
The typical pollinator of a sexually deceptive orchid is a male insect of a species that is polygynous, monandrous,
haplodiploid, and solitary rather than social. Insect behaviours involved in the pollination of sexually deceptive orchids
include pre-copulatory gripping of flowers, brief entrapment, mating, and very rarely, ejaculation. Pollinator behaviour
varies within and among pollinator species.
Deception involving orchid mimicry of insect scent signals is becoming well understood for some species, but
visual and tactile signals such as colour, shape, and texture remain neglected. Experimental manipulations that test
for function, multi-signal interactions, and pollinator perception of these signals are required. Furthermore, other
forms of deception such as exploitation of pollinator sensory biases or mating preferences merit more comprehensive
investigation. Application of molecular techniques adapted from model plants and animals is likely to deliver new
insights into orchid signalling, and pollinator perception and behaviour.
There is little current evidence that sexual deception drives any species-level selection on pollinators. Pollinators do
learn to avoid deceptive orchids and their locations, but this is not necessarily a response specific to orchids. Even in
systems where evidence suggests that orchids do interfere with pollinator mating opportunities, considerable further
research is required to determine whether this is sufficient to impose selection on pollinators or generate antagonistic
coevolution or an arms race between orchids and their pollinators.
Botanists, taxonomists and chemical ecologists have made remarkable progress in the study of deceptive orchid
pollination. Further complementary investigations from entomology and behavioural ecology perspectives should
prove fascinating and engender a more complete understanding of the evolution and maintenance of such enigmatic
plant-animal interactions.
Key words: orchid, pollinator behaviour, mating behaviour, insects, floral scent, colour and shape, mimicry, sensory
biases, learning, evolution.
A. C. Gaskett
34
CONTENTS
I. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
II. Pollination by sexual deception . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
III. Pollinators of sexually deceptive orchids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(1) Pollinator identities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(2) Pollinator mating systems and traits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(3) Exceptions to the general types of pollinators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
IV. Pollinator behaviours . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(1) Pollinator behaviour and the origins of sexual deception . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(2) Pollinator sexual behaviour with orchids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(3) Sexual deception by Pterostylis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(4) Within-species variation in pollinator behaviour . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
V. Pollinator abundance and diversity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VI. Orchid signals and pollinator perception . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(1) Scent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(2) Colour . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(3) Shape . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(4) Multimodal signalling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VII. Pollinator learning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VIII. Costs of deception and orchid-pollinator coevolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
IX. Future directions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
X. Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
XI. Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
XII. References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
I. INTRODUCTION
Sexual deception is an extreme form of pollinator specialisation in which an orchid lures its pollinator with fraudulent sex
signals. Male insects fooled into sexual behaviour with orchid
flowers inadvertently collect or deliver the pollinia and do
not receive floral rewards such as nectar or pollen (Schiestl,
2005; Jersakova, Johnson & Kindlmann, 2006a). In specialist pollination systems, flowers are unconstrained by the
stabilising selection that limits generalist pollination systems
to floral forms suiting several pollinators (Cresswell, 1998;
2000). Instead, specialist flowers can evolve specific fitnessenhancing adaptations to their pollinator, e.g. corollas that
match pollinator tonguelength (Alexandersson & Johnson,
2002; Rodrguez-Girones & Santamara, 2007; Muchhala
& Thomson, 2009). Sexually deceptive orchids are highly
specialised and typically pollinator-specific, thus pollinator
preferences and pollinator-driven selection are likely to be
strongly directional and influence orchid floral features and
diversity (Peakall & Handel, 1993; Cozzolino & Widmer,
2005; Mant, Peakall & Schiestl, 2005d; Schiestl & Schluter,
2009). Investigation of how pollinators and their behaviours
might affect the evolution and maintenance of sexual deception by orchids provides new insights into this intriguing
subject.
Sexual deception relies on the pollinators sexual rather
than foraging behaviour, suggesting insect sexual behaviour
is crucial in driving the evolution of orchid features. Analysis
of the sexual behaviours stimulated by orchids and the natural mating systems of pollinators in the absence of orchids
could reveal which types of insects and behaviours permit and
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Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
35
identifications for some potentially sexually deceptive New
Zealand species are listed in Table 1. The accepted number
of sexually deceptive genera in Australia is likely to change if
reports of sexual deception in Pterostylis cannot be confirmed
or if the proposed taxonomic splitting of Caladenia, Chiloglottis
and Pterostylis is upheld (Jones & Clements, 2003; Jones, 2006;
Hopper, 2009; see Section IV.3; seeTable 1 for synonyms).
In Europe, there is a diversity of food-deceptive orchid
genera, but sexual deception is restricted to the large
and well-studied genus Ophrys, and a single species of the
related and generally food-deceptive genus Orchis (Table 1;
Bino, Dafni & Meeuse, 1982; Widmer, Cozzolino & Dafni,
2001). Perhaps surprisingly, only two South African sexually
deceptive species are known, both from the well-studied and
generally rewarding or food-deceptive genus Disa (Table 1;
Steiner, Whitehead & Johnson, 1994; Johnson, Linder &
Steiner, 1998). Although no sexually deceptive orchids are
known for North America, sexual deception is confirmed
for species from eight South and Central American orchid
genera, and more cases are likely to be recognised in the
future (Table 1; Dodson, 1962; Dod, 1976; Calvo, 1990;
van der Cingel, 2001; Singer, 2002; Singer et al., 2004;
Blanco & Barboza, 2005; Ciotek et al., 2006). For example,
although only two Central American Lepanthes species are
listed in Table 1, this is a diverse and highly regionally
endemic neotropical genus and sexual deception is suspected
for many species (Vasquez et al., 2003; Blanco & Barboza,
2005; Schiestl, 2005). Similarly, there are approximately 15
potentially sexually deceptive Cryptostylis species distributed
throughout the Asia-Pacific region, but sexual deception
is confirmed for only the five species from Australia and
New Zealand (Jones, 2006; Table 1). Subantarctic sexual
deception is also a possibility; Chiloglottis and Pterostylis species
grow on New Zealands subantarctic islands (Meurk, Foggo
& Wilson, 1994; Molloy, 2002).
When data from food- and sexually deceptive orchids
are combined, pollination success is higher in southern
temperate regions (41.4%) than in the tropics (11.5%),
North America (19.5%) and Europe (27.7%; Neiland &
Wilcock, 1998). However, if the dataset includes only
sexually deceptive orchid species (Table 1), there is no
significant difference in the pollination rates of flowers
from southern temperate Australia and from Europe
(mean S.D; Australia: 18.4 11.4%, N = 21; Europe:
18.4 17.4%, N = 8; ANOVA F1,28 = 0.04, P = 0.95).
Insufficient data for sexually deceptive species elsewhere
prevents comparisons for these regions (see Table 1 for a
summary of the pollination rates reported in the literature).
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Diseae
South Africa
Disa atricapilla (Harv. ex
Lindl.)
Disa bivalvata (L. f.)
T.Durand & Schinz.
Diurideae
Australia
Arthrochilus huntianus
(F.Muell.) Blaxell subsp.
huntianus (prev. Spiculaea
huntiana (F.Muell.)
Schltr.)
Arthrochilus irritabilis
F.Muell.
Arthrochilus latipes
D.L.Jones
Caladenia (syn. Arachnorchis)
amnicola D.L.Jones
Caladenia ampla (D.L.Jones)
G.N.Backh.
Caladenia (syn. Arachnorchis)
arenicola Hopper &
A.P.Br.
Caladenia (syn. Arachnorchis)
atrovespa D.L.Jones
Cymbidieae
Japan
Cymbidium pumilum Rolfe
Orchid
Arthrothynnus rufiabdominalis
Brown
Arthrothynnus sp.
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Thynnoides gracilis
(Westwood)
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Thynnoides senilis (Erichson) Tiphiidae;
Hymenoptera
Phymatothynnus nr. nitidus 1 Tiphiidae;
Hymenoptera
Zaspilothynnus nigripes
Tiphiidae;
Guerin
Hymenoptera
Tiphiidae;
Hymenoptera
Arthrothynnus huntianus
Brown (not Rhagigaster
sp. as suggested by
Rotherham, 1967)
Apidae;
Hymenoptera
Sphecidae;
Hymenoptera
Pompilidae;
Hymenoptera
Pollinator
Family; Order
Primarily Podalonia
canescens (Dahlbom)
Hemipepsis hilaris Sm.,
H. capensis Fabricius
unconf.
9.9
36.4 (550 on
184 plants)
42.1 (482 on
116 plants)
% plants
pollinated (N )
Bower (2001a)
Bower (2001a)
Source
Table 1. Sexually deceptive orchid species and their pollinators. All pollinators are male, except where a female pollinator is specifically stated. A question mark (?) after
the pollinator name indicates a putative rather than confirmed pollinator. When data are reported from more than one study, symbols (*, , or ) correspond between the
data and citation. Pro. hybr. after a species name indicates it was described as an occaisional hybrid by Delforge (2005). For Growth form, E = epiphytic, T = terrestrial.
For Pollinator sexual behaviour stimulated by the orchid: 1 = pollinator copulates and ejaculates on the orchid, 2 = pollinator copulates with orchid, 3 = pollinator grips or
lifts hinged labellum, 4 = pollinator is briefly trapped and transfers pollinia on escape, unconf. = pollination is likely to involve sexual behaviour, but this is unconfirmed. %
flowers pollinated is the percentage of flowers that had pollinia collected and deposited, or set fruit. % plants pollinated is the percentage of flowering plants that had pollinia
collected and deposited, or set fruit. (N ) = number of flowers or plants reported in that study. Pollination rates labelled with more than one symbol are the average of data
from the correspondingly labelled source. Australian orchid species names are from the Australian Plant Names Index (Centre for Plant Biodiversity Research, 2008) and
Jones (2006). European orchid species names are according to the International Plant Names Index (2008) and Delforge (2005). Orchid families are according to van der
Cingel (2001) and Kores et al. (2001). European pollinator names are according to Fauna Europaea (2004)
36
A. C. Gaskett
Thynnoides sp.
Phymatothynnus nitidus
Sm.?* Phymatothynnus
victor Turner
Phymatothynnus nr. nitidus 1
Thynnine wasp
Thynnine wasp
Phymatothynnus monilicornis
(Sm.) complex*,
Lophocheilus anilitatus
(Sm.)
Lestricothynnus sp.
Thynnine wasp
Aeolothynnus generosus
(Turner)
Phymatothynnus monilicornis
(Sm.) complex
Zaspilothynnus sp.
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Thynnine wasp
Tiphiidae;
Hymenoptera
Macrothynnus sp.
3?
32.1
14 (782)
1.8*
19.9
Bates (2009a)
Bates (2009b)
Bates (2009a)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Orchid
Table 1. (Cont.)
Thynnine wasp;
Lestricothynnus modestus
(Sm.)*
Campylothynnus assimilis
Sm. C. flavopictus (Sm.)
Phymatothynnus nr. nitidus 1
Thynnine wasp
Zaspilothynnus nigripes
Guerin
Thynnoides gracilis
(Westwood), Thynnoides
spp.
Phymatothynnus sp.
Zeleboria marginalis
(Westwood)* ,
Thynnoides sp. ,
(Thynnoides bidens*
inaccurate; G. Brown
pers. comm.)
Thynnine wasp
Chilothynnus trochanterinus
Brown
Thynnine wasp
Phymatothynnus nr.
pygidialis 1
Thynnine wasp*,
Phymatothynnus ?nitidus
Thynnoides sp.
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Ichneumonid sp.?
Tiphiidae;
Hymenoptera
Ichneumonidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Pollinator
Family; Order
Thynnine wasp
3?
14.4
8.3*
14.3
% plants
pollinated (N )
Coleman (1930)
Bates (2009a)
Source
38
A. C. Gaskett
Zaspilothynnus sp.
Zaspilothynnus sp.
Zaspilothynnus sp.
Phymatothynnus nr. nitidus 1
Guerinius sp.? *, thynnine
wasp
Phymatothynnus monilicornis
(Sm.) complex
Thynnine wasp (Thynnoides
preissi & T. bidens
inaccurate; G. Brown
pers. comm.)
Phymatothynnus nr. nitidus 1
Tachynomyia sp.
Thynnine wasp
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Thynnine wasp
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Thynnine wasp
Tiphiidae;
Hymenoptera
Zaspilothynnus sp.
Thynnine wasp
Tiphiidae;
Hymenoptera
7.3*
Bates (2009b)
(Stoutamire, 1983)
Bates (2009b)
(Pritchard, 2007)
Bates (2009a)
Bates (2009b)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Orchid
Table 1. (Cont.)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Pollinator
Family; Order
Thynnine wasp;
Tiphiidae;
Zaspilothynnus sp.*,
Hymenoptera
Catocheilus affinis
(Guerin)*
Tiphiidae;
Phymatothynnus nr. nitidus
Hymenoptera
1*, Phymatothynnus victor
Zaspilothynnus nigripes
Tiphiidae;
Guerin
Hymenoptera
Zaspilothynnus nigripes
Guerin?
Aeolothynnus sp. (as
Asthenothynnus sp.)
Zeleboria marginalis
(Westwood) (as
Z. marginatus);
Zaspilothynnus nigripes
Guerin*
Lophocheilus anilitatus (Sm.)
T
Thynnine wasp*;
Phymatothynnus ?nitidus
Tachynomia sp.
Phymatothynnus sp.
2.5*
% plants
pollinated (N )
Bates (2009a)
Bates (2009a)
Bower (2001b)
Source
40
A. C. Gaskett
Thynnoides mesopleuralis
Turner
Thynnine wasp
Phymatothynnus monilicornis
(Sm.)*, Phymatothynnus
monilicornis complex*,
Phymatothynnus sp. 14
Thynnine wasp
Thynnoides aff. gracilis
Thynnoides pugionatus
Guerin (sp. complex),
T. rufithorax Turner,
Thynnoides gracilis
(Westwood)
Thynnoides new sp.
D Brown
Phymatothynnus nr. nitidus 1
Macrothynnus sp.
Aeolothynnus generosus
(Turner)
Thynnine wasp
Phymatothynnus sp.
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Phymatothynnus nitidus
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Thynnine wasp
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
20.5
36.4 (1214)*
Bates (2009a)
Bates (2009b)
Bates (2009a)
Bates (2009a)
Bates (2009a)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Scoliidae;
Hymenoptera
Scoliidae;
Hymenoptera
Scoliidae;
Hymenoptera
Scoliidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Thynnine wasp
Lophyrotoma leachii (Kirby)*, Pergidae;
Pterygophorus sp. ,
Hymenoptera
L. cyanea (Leach)
Iswaroides armiger (Turner) Tiphiidae;
(prev. Thynnoturneria
Hymenoptera
armiger)
Scoliidae;
Hymenoptera
Thynnine wasp
Campsomeris sp.
Campsomeris tasmaniensis
Saussure
Scoliid wasp (also
self-pollinates)
Scoliid wasp (also
self-pollinates)
Scoliid wasp (also
self-pollinates)
Neozeleboria n.sp. 33 Brown
Phymatothynnus ?nitidus
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Pollinator
Family; Order
Calochilus cupreus
R.S.Rogers
Calochilus platychilus
D.L.Jones
Calochilus pruinosus
D.L.Jones
Chiloglottis anaticeps
D.L.Jones
Chiloglottis (syn. Simpliglottis)
chlorantha D.L.Jones
Chiloglottis diphylla R.Br.
Orchid
Table 1. (Cont.)
% plants
pollinated (N )
Bates (2009a)
Bates (2009a)
Bower (2001c)
Bates (2009a)
Bates (2009a)
Source
42
A. C. Gaskett
Thynnine wasp
Neozeleboria n.sp. 29 Brown
Chiloglottis (syn.
Sympliglottis) triceratops
D.L.Jones
Chiloglottis sphrynoides
D.L.Jones
Chiloglottis sylvestris
D.L.Jones &
M.A.Clem.
Chiloglottis (syn. Myrmechila)
trapeziformis Fitzg.
Chilothynnus palachilus
Brown
T
Neozeleboria carinicollis
Turner
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Neozeleboria nr impatiens 2
Neozeleboria nr monticola 1
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Eirone sp.
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
4*
58.5 (210)
58.5 (210)
Stoutamire (1975)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Cryptostylis hunteriana
Nicholls
Cryptostylis leptochila Benth.
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Thynnine wasp
Tiphiidae;
Hymenoptera
Neozeleboria proxima
(Turner)
Pollinator
Family; Order
Orchid
Table 1. (Cont.)
1?
35.0 (47)*
66.6 (154)*
25.4 (994)*
41.9 (121)*
70.4 (402)*
41 (86)*
% plants
pollinated (N )
12.0 (122)*
5.2 (1329)*
32.7 (2187)*
41 (86)*
Source
44
A. C. Gaskett
Diptera
Mycetophilidae;
Diptera
Zaspilothynnus sp.
Myrmecia urens Lower
Mycetophilid fly
Thynnine wasp
Erione sp.*, Labium sp.
Culex sp. female mosquito?
Mycetophilid fly
Fly
Fly
Fly
Mycomya sp.
T
T
Zaspilothynnus nigripes
Guerin, Z. dilatatus
spiculifera Turner
Thynnine wasp
Diptera
Diptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Formicidae;
Hymenoptera
Mycetophilidae,
Diptera
Tiphiidae,
Hymenoptera
Tiphiidae,
Ichneumonidae;
Hymenoptera
Culicidae; Diptera
Mycetophilidae,
Diptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Tiphiidae;
Hymenoptera
Zaspilothynnus trilobatus
Turner*, Z. dilatatus
spiculifera Turner
Zaspilothynnus nigripes
Guerin* (Thynnoides
bidens inaccurate, G.
Brown pers. comm.),
Thynnoides elongata
Zaspilothynnus nigripes
Guerin
unconf.
unconf.
unconf.
unconf.
unconf.
unconf.
unconf.
23.9 (1478)
20.7 (1203)
20.7 (1203)
Bernhardt (1995)
Bates (2009a)
Bates (2009a)
Bates (2009b)
Coleman (1934)
Bates (2009a)
Bates (2009b)
Bates (2009a)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Pterostylis psammophila
(D.L.Jones) R.J.Bates
(syn. Oligochaetochilus
psammophilus)
Pterostylis pusilla
R.S.Rogers (syn
Oligochaetochilus pusillus
(R.S.Rogers) Szlach.)
Pterostylis (syn. Diplodium)
rogersii E.Coleman
Pterostylis falcata
R.S.Rogers
Pterostylis gibbosa R.Br. (syn.
Oligochaetochilus gibbosus)
Pterostylis lepida (syn.
Oligochaetochilus lepidus)
Pterostylis (syn. Linguella)
nana R.Br.
Pterostylis nutans R.Br.
Orchid
Table 1. (Cont.)
Diptera
Tiphiidae;
Hymenoptera
Fly
Fly
Gnat
Thynnoturneria sp.*,
Iswaroides sp.
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Diptera
Diptera
Diptera
Gnat
Mycetophilidae;
Diptera
Diptera
Fungus gnat
Mycetophilidae;
Diptera
Mycetophilidae;
Diptera
Diptera
Fungus gnat
Fungus gnat
Fly
Mosquito-like fly
Mycetophilid fly
Heteropterna sp.
Mycetophilidae;
Diptera
Mycetophilidae,
Diptera
Diptera
Pollinator
Family; Order
unconf.
unconf.
unconf.
unconf.
unconf.
unconf.
4?
unconf.
4?
unconf.
unconf.
4?
unconf.
12 (490)*
25.9 (154)*
% plants
pollinated (N )
Sargent (1909)
Sargent (1909)
Bates (2009b)
Bates (2009a)
Hyett (1960)
Coleman (1934)
Source
46
A. C. Gaskett
Tachinidae;
Diptera
Meliponinae;
Hymenoptera
Paragymnomma sp.
Plebeia droryana Friese
Tachinid fly
Centris sp.?
Telipogon sp.
Stellilabium sp.
Apidae;
Hymenoptera
Tachinidae;
Diptera
Tachinidae;
Diptera
Anthophoridae;
Hymenoptera
Nannotrigona testaceicornis
(Lep.), Scaptotrigona sp.
Tachinid fly
Sciaridae; Diptera
E
E
Scoliidae;
Hymenoptera
Campsomeris bistrimacula
(Lep.)
Fungus gnat?
Mycetophilidae,
Keratophilidae;
Diptera
Mycetophilidae;
Diptera
Zygomyia sp.
Ichneumonidae;
Hymenoptera
Mycetophilidae;
Diptera
New Zealand
2&4
unconf.
1?
?
unconf.
4?
4?
4?
4?
1?
41 (24)
11.6 (4232 on
200 plants)
52.2 (57)
<25%
41.6 (24)
2.8 (72)
52.2 (57)
41.6 (24)
2.8 (72)
Singer (2002)
Graham (1983)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Colletes sp.
Andrena paucisquama
Noskiewicz
Andrena flavipes Panzer
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Colletidae;
Hymenoptera
Megachilidae;
Hymenoptera
Anthophora orientalis
Morawitz
Eucera taurica Morawitz
Pollinator
Family; Order
Orchideae
Europe
Orchid
Table 1. (Cont.)
% plants
pollinated (N )
Delforge (2005)
Delforge (2005)
Paulus (2006)
Delforge (2005)
Delforge (2005)
Delforge (2005)
Source
48
A. C. Gaskett
Chalicodoma parietina
(Geoffr.), C. pyrenaica
(Lep.)
Chalicodoma sicula (Rossi)*,
C. benoisti Tkalcu
Chalicodoma parietina
(Geoffr.)
Ophrys bertoloniiformis
O.Danesch &
E.Danesch
Ophrys biancae Macch.
Chalicodoma parietina
(Geoffr.), C. pyrenaica
(Lep.)
Andrena hattorfiana
(Fabricius)
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Megachilidae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Megachilidae;
Hymenoptera
Chalicodoma parietina
(Geoffr.)
Eucera seminuda Brulle
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Delforge (2005)
Delforge (2005)
Delforge (2005)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Ophrys bornmuelleri
M.Schulze
Ophrys bucephala Golz &
H.R.Reinhard
Ophrys calocaerina
Devillers-Tersch. &
Devillers
Ophrys calypsus M.Hirth &
H.Spaethe
Ophrys candica Greuter,
Matthas & Risse
Ophrys celiensis (O.Danesch
& E.Danesch)
P.Delforge
Ophrys cerastes Devillers &
Devillers-Tersch. (as
Ophrys cornuta (small)?*)
Ophrys ceto P. Devillers,
Devillers-Tersch. &
P.Delforge
Ophrys chestermanii (J.J.
Wood) Golz &
H.R.Reinhard
Ophrys cilentana
Devillers-Tersch. &
Devillers
Ophrys cilicica Schltr.
Orchid
Table 1. (Cont.)
Sphecidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Argogorytes sp.
Andrenidae;
Hymenoptera
Apidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Eucera hispana Lep.*,
Anthophoridae;
E. hispana Lep.
Hymenoptera
Eucera graeca Radoszkowski Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Scarabaeidae;
Coleoptera
Anthophoridae;
Hymenoptera
Pollinator
Family; Order
Delforge (2005)
Delforge (2005)
Delforge (2005)
2
2
Delforge (2005)
Source
Paulus (2001); Delforge (2005)
% plants
pollinated (N )
2
21.3 (122 on 37
plants)*
50
A. C. Gaskett
Chalicodoma parietina
(Geoffr.)
Chalicodoma manicata
(Giraud)
Eucera dimidiata Brulle
Anthophoridae;
Hymenoptera
Colletidae;
Hymenoptera
Megachilidae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Megachilidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Apidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Megachilidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Apidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Delforge (2005)
Delforge (2005)
Delforge (2005)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Ophrys grigoriana
G.Kretzschmar &
H.Kretzschmar
Ophrys hebes (Kalopissis)
E.Willing & B.Willing
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Chalicodoma sp.
Andrenidae;
Hymenoptera
Pollinator
Family; Order
Ophrys fuciflora
(F.W.Schmidt) Moench
Orchid
Table 1. (Cont.)
5.45 (463
plants)
% plants
pollinated (N )
Paulus (2006)
Delforge (2005)
Delforge (2005)
Paulus (2006)
Delforge (2005)
Delforge (2005)
Paulus (2000)
Source
52
A. C. Gaskett
Ophrys israelitica
H.Baumann & Kunkele
Ophrys kotschyi H.Fleischm.
& Soo
Ophrys lacaitae Lojac.
Ophrys insectifera L.
T
Andrena schulzi Strand
Argogorytes fargeii
(Shuckard), A. mystaceus
(L.), Anthobium minutum
F. (Staphylinidae)
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Colletidae;
Hymenoptera
Andrenidae;
Hymenoptera
Sphecidae;
Hymenoptera,
Staphylinidae;
Coleoptera
Anthophoridae;
Hymenoptera,
Scarabaeidae;
Coleoptera
Syrphidae;
Diptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Apidae;
Hymenoptera
15.1 (2775
plants)*
Agren
& Borg-Karlson (1984);
Delforge (2005);
Vanderwoestijne et al. (2009)*
Paulus & Gack (1990)
Paulus (2006)
Delforge (2005)
Delforge (2005)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Ophrys marmorata
G.Foelsche &
W.Foelsche
Ophrys massiliensis Viglione
& Vela
Ophrys melitensis (Salk.)
Devillers-Tersch. &
Devillers
Ophrys lupercalis
Devillers-Tersch. &
Devillers
Ophrys lutea Cav.
Orchid
Table 1. (Cont.)
Megachilidae;
Hymenoptera
Colletidae,
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrena flavipes
A. Andrenidae;
creberrima Perez ?
Hymenoptera
Andrena ocreata (Christ)?
Andrenidae;
Hymenoptera
Andrena labialis (Kirby)
Andrenidae;
Hymenoptera
Panzer* ,
Pollinator
Family; Order
Andrena vulpecula
Kriechbaumer
Anthophora cf. mucida
% plants
pollinated (N )
Delforge (2005)
Delforge (2005)
Delforge (2005)
Delforge (2005)
Source
54
A. C. Gaskett
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Colletidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Apidae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrena thoracica
(Fabricius)* , A. florentina
Magretti*, A. sabulosa
(Scopoli) , A. sabulosa
subsp. trimerana*
Andrena thoracica
(Fabricius), A. nigroaenea
(Kirby)?
Eucera nigrescens Perez
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Colletidae;
Hymenoptera
Ophrys omegaifera
H.Fleischm. (as O.
omegifera subsp. omegaifera
in Paulus & Gack 1990)
Ophrys omegaifera subsp.
dyris (Maire) Del Prete
Ophrys ortuabis M.P.Grasso
& Manca
Ophrys oxyrrhynchos Tod.
Ophrys montis-leonis
O.Danesch &
E.Danesch (pro hybr.)
Ophrys morio Paulus &
Kreutz
Ophrys morisii (Martelli)
G.Keller & Soo
Ophrys murbeckii
H.Fleischm.
Ophrys neglecta Parl.
Delforge (2005)
Delforge (2005)
Paulus (2006)
Delforge (2005)
Delforge (2005)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Andrena panurgimorpha
Mavromoustakis,
A. humilis Imhoff,
A. tadauchii
Gusenleitner, A. clypella
subsp. hasiata?
Eucera barbiventris Perez
Chalicodoma albonotata
(Radoszkoski)
Eucera longicornis (L.)*,
Eucera nigrescens Perez*,
E. interrupta Baer*,
E. (Hetereucera) elongatula
Vachal
Eucera clypeata Erichson
Andrena hesperia Sm.* ,
A. vulpecula
Kriechbaumer* ,
A. merula War. ,
A. taraxaci Giraud ,
A. bicolor Fabricius ?
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Megachilidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Osmia mustelina
Gerstaecker
Ophrys promontorii
O.Danesch &
E.Danesch
Ophrys provincialis
(Baumann & Kunkele)
Paulus
Ophrys reinholdii Spruner
ex. Boiss.
Anthophoridae;
Hymenoptera
Megachilidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Pollinator
Family; Order
Orchid
Table 1. (Cont.)
% plants
pollinated (N )
Paulus (2000)
Delforge (2005)
Delforge (2005)
Delforge (2005)
Delforge (2005)
Delforge (2005)
Source
56
A. C. Gaskett
Ophrys subinsectifera
C.E.Hermos. &
J.Sabando
Ophrys sulcata
Devillers-Tersch. &
Devillers
Ophrys tardans O.Danesch
& E.Danesch (pro
hybr.)
Ophrys tarentina Golz &
H.R.Reinhard
Ophrys tarquinia P.Delforge
Andrena nigroaenea
(Kirby)* , A. barbilabris
(Kirby)*, A. thoracica
(Fabricius)*, A. cineraria
(L.)*, A. limata
Eversmann*
Megachilidae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Andrenidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Argidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
Scoliidae;
Hymenoptera
Scoliidae;
Hymenoptera
Anthophoridae;
Hymenoptera
Andrenidae;
Hymenoptera
55.5 (20 on 13
plants)*
0.03 (3000)
12.9 (963)*
17.04 (235
plants)
Delforge (2005)
Delforge (2005)
Delforge (2005)
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Ophrys transhyrcana
Czerniak.
Ophrys umbilicata Desf.
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Ophrys zonata
Devillers-Tersch. &
Devillers
Orchis galilaea (Bornmuller
et Schultze) Schlechter
Pollinator
Family; Order
Lassioglossum marginatum
Brulle (prev. Halictus
(Evylaeus) marginatus)
Halictidae;
Hymenoptera
Apidae;
Tetraloniella fulvescens
Hymenoptera
(Giraud) (prev.
Tetralonia f.)*, Eucera
fulvescens Giraud , Eucera
inulae , but reports of
Tetralonia ruficornis
(Fabricius) are
inaccurate .
Andrena bicolor Fabricius?
Andrenidae;
Hymenoptera
Andrena pandellei Perez
Andrenidae;
Hymenoptera
Andrena morio Brulle*,
Andrenidae;
A. fuscosa Erichson ?
Hymenoptera
Eucera gaullei Vachal,
Anthophoridae;
E. galilaea Tkalcu,
Hymenoptera
E. spatulata Gribodo,
E. seminuda Brulle,
Eucera penicillata Risch
Eucera clypeata Erichson
Anthophoridae;
Hymenoptera
Scarabaeidae;
Blitopertha nigripennis
Reitter
Coleoptera
Dasyscolia ciliata (Fabricius) Scoliidae;
(as Campsoscolia ciliata)
Hymenoptera
Anthophoridae;
Eucera dimidiata Brulle,
Hymenoptera
E. bidentata Perez,
E. nigrilabris Lep.
E. rufitarsis Friese,
E. curvitarsis Mocsary?
Andrena flavipes Panzer
Andrenidae;
Hymenoptera
Ophrys tetraloniae
W.P.Teschner
Orchid
Table 1. (Cont.)
27.5 (2146 on
129 plants)*
Delforge (2005)
Paulus (2009)
Paulus (2000)
Delforge (2005)
Source
Delforge (2005)
% plants
pollinated (N )
58
A. C. Gaskett
59
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A. C. Gaskett
60
deceptive pollination systems require further substantiation.
For example, early reports of pollination of Pterostylis orchids
by female mosquitoes (Coleman, 1934; Table 1) are drawn
from a single observation and it is unclear whether pollination
involved any sexual behaviour.
Solitary and parasitoid wasps are the most common
pollinators of sexually deceptive orchids (Table 1). Such
specialised pollination by wasps is rare in almost all other
groups of flowering plants, except figs (Johnson, 2005). Thynnine, ichneumonid and scoliid wasps pollinate species from
most Australian sexually deceptive orchid genera, and sphecid and pompilid wasps pollinate both South African Disa
species (Table 1). European Ophrys species are predominantly
pollinated by solitary bees in the families Andrenidae, Colletidae, Megachilidae, and Apidae (but see Section III.3, and
families of pollinators listed in Table 1).
(2) Pollinator mating systems and traits
The mating systems of solitary wasps and bees have several
features that may facilitate or pre-adapt them to exploitation by sexually deceptive orchids. These features could
include: male attraction to airborne sex signals (allowing
signal mimicry and long-distance attraction by orchids),
intense male searching and vigilance for sex signals (encouraging rapid response to orchids), vigorous mating behaviour
(ensuring pollinia transfer between the orchid and pollinator), and polygyny (ensuring a pollinator visits more than one
orchid, permitting both pollinia collection and delivery). Correspondingly, male hymenoptera from solitary species are
typically strongly attracted to the airborne sex pheromones
of female conspecifics (Ridley, 1993; Godfray & Cook, 1997).
Male bees and wasps are also generally highly vigilant and
respond quickly to female pheromones. Female monandry
or first-male sperm precedence imposes strong selection on
these males to secure paternity by rapidly finding and mating
with virgin females (Alcock et al., 1978; Eickwort & Ginsberg,
1980; Tengo, Eriksson & Borg-Karlson, 1989; Hardy, 1994;
El Agoze, Poirie & Periquet, 1995; Paxton, 2005; Damiens &
Boivin, 2006). For example, the solitary bee Colletes cunicularius is the sole or major pollinator for at least six Ophrys species
(Table 1) and has many characteristics lending it to orchid
exploitation. Colletes cunicularius is relatively common, the
females are monandrous, attract mates with sex pheromones
and do not exhibit any courtship behaviour, and the males
are polygynous (Larsson, 1991). Consequently, there is considerable competition between male Colletes cunicularius to
detect virgin female pheromones quickly and to mate first
with many females (Cane & Tengo, 1988; Mant et al., 2005b).
Male thynnine wasps (family Tiphiidae, subfamily Thynninae), which are common pollinators of Australian sexually
deceptive orchids, have many similar mate-searching and
mating behaviours (Burrell, 1935; Alcock, 1981). Therefore,
orchids that mimic the sex pheromones of females of these
insect taxa benefit from the males rapid response to scent
and enthusiastic and somewhat indiscriminate mating.
Haplodiploid insects are surprisingly common pollinators of sexually deceptive orchids (Gaskett et al., 2008). Many
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
Orchideae
Orchis israelitica
H. Baumann & Dafni
Neottieae
Cephalanthera rubra
(L.) Rich.
Diurideae
Diuris aequalis Fitzg.
Scabiosa columbaria L.
(Dipsacaceae)
Rewarding model
Diseae
Disa cephalotes Rchb.f. ssp.
cephalotes
Orchid species
Solitary bees
Chelostoma fuliginosum Panzer,
C. campanularum (Kirby)
(Megachilidae)
Solitary bees
Anthophora sp., Eucera clypeata
Erichson (Anthophoridae),
Bombylius sp. (Bombyliidae)
Pollinator
Nilsson (1983)
Johnson (2000)
Johnson (1994)
Source
Table 2. Food-deceptive or unrewarding orchid species tested for similarity in colour with co-occurring plant species that do provide nectar rewards for their pollinators.
Pollination success for each orchid species is the average of values reported in the source. N = sum of sample sizes reported in source
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IV. POLLINATOR BEHAVIOURS
(1) Pollinator behaviour and the origins of sexual
deception
Although pollination by sexual deception is confirmed only
for orchids, Kullenberg (1961) reported sexual behaviour by
a sphecid wasp on flowers of Guiera senegalensis Lam. (Combretaceae), and sexual deception is a compelling explanation
for the nectarless and insectiform flowers of Gilliesia graminea
Lindl. (Alliaceae; Rudall et al., 2002). These cases are yet
to be confirmed. Despite the prevalence of sexual deception amongst orchids, pollination involving some form of
insect sexual behaviour may actually precede the evolution
of orchids. Early floral scents that evolved initially to protect
plant reproductive organs from insect herbivory are likely to
have resembled the sex pheromones of other insects (Pellmyr
& Thien, 1986; Harrewijn, Minks & Mollema, 1994; Gang,
2005). Consequently, insects were likely to aggregate and
mate on the flowers and coincidentally function as pollinators. For many modern insect species, plant and floral scents
still enhance insect sex pheromone release and attractiveness
(for a review, see Reddy & Guerrero, 2004).
In orchids, phylogenetic comparisons suggest that sexual
deception evolved from food deception, which is common
amongst primitive, nectarless orchid groups, although some
of these orchids do provide pollen as a reward (Cozzolino
& Widmer, 2005). Surveying pollinator behaviour within
a genus could indicate how the transition between food
and sexual deception may occur, and whether elements of
both systems can coexist. For example, different species of
Australian Donkey orchids (genus Diuris) reward or deceive
foraging insects, and potentially, exploit aspects of pollinator
sexual behaviour. Diuris alba appears to be a rewarding
species; it provides nectar and has several pollinator species,
whereas Diuris aurea is food-deceptive and has several
pollinators (Indsto et al., 2007). Diuris pedunculata provides
nectar, but an early report suggests it is pollinated only by
male bees of Halictus lanuginosus, perhaps suggesting some sexbased pollination (Coleman, 1932). Another species, Diuris
maculata, has features associated with sexual deception, it lacks
nectar and is pollinated by male bees of Tricholetes venustus
(Indsto et al., 2006). However, detailed observations reveal
no sexual behaviour with orchid flowers (Indsto et al., 2006).
In the case of these last two species, Diuris pedunculata and
D. maculata, it seems likely that the orchids act as, or mimic,
both a foraging site and a rendezvous site for courting
males, although the lack of female visitors is inconsistent
with observations of other orchid species thought to mimic
rendezvous sites (Nilsson, 1983; Steiner, 1998; Jersakova
et al., 2006a). Other orchids proposed to employ aspects
of both pollinator foraging and sexual behaviour include
European Orchis galilaea (Bino et al., 1982) and Australian
Caladenia patersonii (Stoutamire, 1983), and possibly Japanese
Cymbidium pumilum (Sasaki et al., 1991). However, there is
scarce empirical evidence that any orchid species employs
both sexual and food deception (Faast et al., 2009). In
these putative cases, detailed observations are required to
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63
Table 3. Percentages of insects fooled into pollinating some Australian sexually deceptive orchids after initial attraction. N = visits
observed. *Data combined & averaged from more than one study
% fooled
Orchid
Pollinator behaviour
7.5
41.7
10.5
28.3
3.85
24.0
39.0
23.23
287
24
38
53
26
48
79
2897
13.0
90.99
46
111
Cryptostylis subulata
Copulation/ejaculation
92.73
55
Drakaea glyptodon
Leporella fimbriata
Spiculaea ciliata
21.9
60.0
44.0
618
55
50
Source
Peakall & Beattie (1996)
Bower (1996)
Bower (1996)
Bower (1996)
Bower (1996)
Bower (1996)
Bower (1996)
Peakall & Handel (1993);
Bower (1996)
Bower (1996)
Gaskett et al. (2008); A. C.
Gaskett (unpublished
data)
Gaskett et al. (2008); A. C.
Gaskett (unpublished
data)
Peakall (1990)
Peakall (1989)
Alcock (2000)
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64
depending on the extent of pollinator sexual behaviour
elicited by each orchid (Table 3). If orchids are classified
according to the sexual behaviour stimulated from their pollinators, orchids causing more extreme behaviour have higher
pollination success (Gaskett et al., 2008). Analysis of data
presented in Tables 1 and 3 shows there is also a significant
correlation between the extent of pollinator sexual behaviour
and the percentage of attracted insects that are fooled into
pollination (partial correlation: 0.84, d.f. = 11, P < 0.001,
controlling for the number of pollinator visits observed).
Orchids stimulating the most extreme sexual behaviour are
the most likely to fool pollinators into making contact with
the orchid (Fig. 3), suggesting a strong fitness benefit for
orchids with persuasive mimicry and deceptive signalling. It
is worth noting that the data available in the literature may
overestimate pollinator response because it is impossible to
determine the number of insects that did detect an orchid,
but did not fly close enough to be counted by an observer.
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65
females of their pollinator species using gas chromatography
and gas chromatography-mass spectrometry (GC, GC-MS;
Bergstrom, 1978; Borg-Karlson, 1987, 1990; Schiestl et al.,
1999; Flach et al., 2006). The most powerful studies combine
behavioural observations and assays with chemical analyses
and tests for pollinator perception of scents (Fig. 4). Pollinator
perception of orchid scent can be tested with calcium imaging of antennal lobe activity in the pollinator brain during
exposure to floral scents (Galizia et al., 2005), or much more
commonly, gas chromatography-electroantennography or
gas chromatography-electroantennal detection (GC-EAG or
GC-EAD; Schiestl, 2005). GC-EAD allows scent compounds
to be individually tested for their capacity to stimulate a pollinators antennal chemoreceptors. Whole live antennae of the
pollinator species have been tested with synthetic versions
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66
Fig. 4. Research paths incorporating a pollinator perspective: assessing the role of pollinator behaviour and pollinator perception
of orchid signals in studies of pollination by sexual deception. GC, gas chromatography; GC-MS, gas chromatographymass spectrometry; GC-EAD, gas chromatography-electroantennodetection or gas chromatography-electroantennography; SEM,
scanning electron microscopy. 1 Bower & Brown (2009); 2 Peakall (1990); 3 Alcock (1981); 4 Schiestl et al. (1999); 5 Schiestl et al. (2003);
6
Gaskett & Herberstein (2010); 7 Schiestl (2004); 8 Zelditch et al. (2004); 9 Shipunov & Bateman (2005); 10 Kullenberg (1961); 11 Agren
12
13
14
15
16
et al. 1984; Galizia et al. (2005); Francke et al. (2009); Vereecken et al. (2007a); Ayasse et al. (2000); Wong et al. (2004);
17 Johnson et al. (1998); 18 Mondrag
on-Palomino & Theien (2009); 19 Fitzpatrick et al. (2005).
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67
peak sensitivities of the visual receptors of common sexually
deceptive orchid pollinators such as Colletes cunicularius or
thynnines (e.g. Peitsch et al., 1992). More studies investigating and testing for exploitation of sensory biases and sensory
traps in sexual deception would be fascinating.
Finally, although within-species colour polymorphism
occurs in several food-deceptive orchid species (Gigord,
Macnair & Smithson, 2001; Koivisto, Vallius & Salonen,
2002; Salzmann & Schiestl, 2007), it is rare for sexually
deceptive species, exceptions include Caladenia behrii (Dickson
& Petit, 2006) and Ophrys arachnitiformis (Vereecken & Schiestl,
2009). The role of colour polymorphism is unclear. Most
studies with food-deceptive orchid species find no evidence
of frequency-dependent selection or pollinator preferences
for rare colour morphs (Koivisto et al., 2002; Aragon &
Ackerman, 2004; Pellegrino, Bellusci & Musacchio, 2005;
Dickson & Petit, 2006; Smithson et al., 2007; Tremblay
& Ackerman, 2007; Vereecken & Schiestl, 2009, but see
Gigord et al., 2001). Thus future studies of colour polymorphism in either sexual or food deception should consider
how colour morphs differ in several factors such as fitness,
post-pollination barriers, or seed viability, rather than just
pollinator preferences (e.g. Pellegrino et al., 2005; Jersakova,
Kindlmann & Renner, 2006b).
(3) Shape
The shape of an orchid is also likely to be an important visual
signal for sexual deception. After a pollinator has landed on
an orchid, he must be persuaded into attempting copulation.
This could be accomplished by close-range mimicry of the
dimensions and tactile qualities of the body of his female
conspecific. Comparison of the distribution and dimensions
of hairs on the surface of Ophrys spp. labellae and the specific female bees they mimic indicated considerable textural
congruence (Agren,
Kullenberg & Sensenbaugh, 1984). Several orchids feature notably insectiform labellae that might
mimic female insects, e.g. Drakaea, Paracaleana and Lepanthes
species and Geoblasta pennicillata, but others, including Cryptostylis erecta, Trigonidium obtusum and Pterostylis species do not
appear insect-like to the human viewer (Figs 1 and 2; Singer,
2002; Blanco & Barboza, 2005; Ciotek et al., 2006).
Close-range tactile cues may also determine pollinator
orientation on the orchid, which is imperative for effective
pollinia collection and transferral (Kullenberg, 1961;
Wallace, 1978). The surface cells on the labellae of Ophrys fusca
and O. lutea bear long trichomes that point towards the stigma
and these are likely to direct the pollinators genital claspers
towards the stigma and adjacent pollinia (Ascensao et al.,
2005). The hypothesis that trichomes function in pollinator
orientation could be tested by experimentally removing
trichomes or manipulating their arrangement and observing
pollinator orientation behaviour. In field experiments,
pollination and fruit-set would be expected to be lower
for orchids with experimentally removed trichomes than for
control orchids that had received a similar level of sham
damage, e.g. small cuts made on the reverse of the labellum.
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68
Shape may also exploit pollinator sensory biases involved
in male mate-choice behaviour. Morphological comparisons
of flower size show that the labellae of Chiloglottis trapeziformis
orchids are consistently larger than the female insects they
mimic (Schiestl, 2004). These large flowers could represent
an irresistible super-stimulus that exploits the common male
insect preference for large female body size (Bonduriansky,
2001; Schiestl, 2004). However, in tests with model female
insects, the pollinator of Chiloglottis trapeziformis preferred
intermediate over both small and large models, indicating a
limit to male preference for large female body size (Schiestl,
2004). However, measurements were taken of the whole
labellum, rather than just the insectiform black, glossy calli
on the labellum of the orchid, which may be more relevant
for comparison (for drawings, see Bower, 1996; Mant et al.,
2005a; Mant, Peakall & Weston, 2005e). A comprehensive
multi-species study of the possible tactile function of these
calli in Chiloglottis species, the insectiform labellae of Drakaea
or Caleana species, or the whole labellae of Ophrys species,
would help clarify the role (if any) of shape mimicry in sexual
deception.
The literature suggests two alternative and as yet unstudied
hypotheses for the function of varying floral shape in sexual
deception. Firstly, variation in shape could impair pollinator learned avoidance in the same way that scent variation
hinders this process in Ophrys sphegodes and other unrewarding flowers (Ayasse et al., 2000; Raguso, 2004). Experiments
testing pollinator behaviour and learning using manipulated
floral shapes are required. Secondly, floral shape is undoubtedly important in the function of floral traps. Morphology is
likely to control both the location and duration of pollinator
contact, with strong consequences for pollinia transfer and
thus fitness. For example, when a pollinator grips the loosely
hinged labellum of Arthrochilus species, he is swiftly swung into
a pair of flanges that control his position against the stigma
and pollinia, then a second pair of curved flanges traps his
wings and prevents his escape until after pollinia transferral
has occurred (Bower, 2001a). Research into the evolution of
trap mechanisms could commence by testing how variation
in some morphological aspect of a floral trap interacts with
pollination success.
(4) Multimodal signalling
In his thesis, Kullenberg (1961) addressed the possibility
of multiple olfactory, visual and tactile stimuli in sexual
deception, but there are few contemporary tests in which
pollinators are offered models with simultaneous variations
in scent and colour and shape to determine signal functions
and the extent of signal duplication (Fig. 4; e.g. Vereecken &
Schiestl, 2009). Sexually deceptive orchids exploit their pollinators mate search and mate-choice signals. Mate choice
often involves assessment of multicomponent or multimodal
signals (Candolin, 2003; Papke, Kemp & Rutowski, 2007).
Therefore, orchids that produce multiple deceptive sex signals in chemical, visual and tactile modes could be extremely
compelling for their mate-searching potential pollinators.
Since the attractiveness of an orchid appears to provide
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69
et al., 2006a). Pollinators of sexually deceptive orchids receive
no benefits from orchid visits and in most cases orchids are
also unlikely to impose any costs on their pollinator species.
Costs to the pollinator species are unlikely because not all
insects that encounter an orchid are fooled into acting as
pollinators (Table 3) and orchids are often rare or flower
sporadically, and do not grow in all areas in which their pollinating species is found (Kindlemann & Balounova, 2001;
Peakall et al., 2002; Bower & Brown, 2009). Therefore many
insects from a pollinating species may never or only rarely
encounter a sexually deceptive orchid. In this situation, at
species level the fitness benefits of responding swiftly to sexual
signals and securing many matings with real females may outweigh the disadvantages of occasional matings with orchids.
However, mating with orchids may have negative impacts
for individual pollinators. Pollinators can prefer orchids to
real females, prematurely end a copulation with a real female
to visit an orchid, or be unable to find real female mates
among false orchid signals (Coate, 1965; Wong & Schiestl,
2002; Schiestl, 2004; Vereecken & Schiestl, 2008). If sexual
deception involves pollinator ejaculation and sperm wastage,
it may even affect a males future mating opportunities.
Sperm production can be costly in many insect species,
limiting lifetime sperm production (Wedell, Gage & Parker,
2002). Even if sperm production is not costly, a male could
become temporarily sperm depleted during an orchid visit. If
he then soon encounters a real female, he may not be able to
copulate, or may transfer only seminal fluid without sperm,
as occurs in many solitary wasp species (King, 1987; Damiens
& Boivin, 2006). Although currently considered extremely
rare and proposed only for Cryptostylis and Lepanthes species
(Blanco & Barboza, 2005; Gaskett et al., 2008), pollinator
ejaculation has been studied in so few species that it may be
more common than currently believed.
Learned avoidance by pollinators after visiting sexually
deceptive orchids is well documented and could be an
evolved response to the costs imposed by the deception
(Peakall, 1990; Bower, 1996; Bower & Brown, 1997; Alcock,
2000; Ayasse et al., 2000; Wong & Schiestl, 2002; Wong et al.,
2004). If the costs of deception are strong enough to select
for pollinator avoidance of orchids, this in turn could select
for increasingly sophisticated and persuasive orchid signals,
ie. antagonistic coevolution or an arms race (Wong & Schiestl, 2002; Gaskett et al., 2008). However, there are currently
no data indicating whether these costs actually do impede
pollinator fitness sufficiently to drive coevolution and a large
study addressing pollinator mating behaviour and sperm use
and exploring the potential for individual and population
costs of exploitation by orchids is required.
At present, it is apparent that pollinators and their
behaviours, mating systems, diversity, and sensory perception could exert considerable selection on sexually deceptive
orchids and their signals. If sexual deception does negatively
impact the mating opportunities of individual pollinators,
there is no experimental evidence to show this is adequate to
impose selection on pollinators at the level of population or
Biological Reviews 86 (2011) 3375 2010 The Author. Biological Reviews 2010 Cambridge Philosophical Society
A. C. Gaskett
70
species. Thus, antagonistic coevolution remains a tantalising
hypothesis for future research.
X. CONCLUSIONS
(1) At present, sexually deceptive orchid genera have
been identified in Australia and New Zealand
(2)
(3)
(4)
(5)
(6)
(7)
(8)
XI. ACKNOWLEDGEMENTS
I thank Marie Herberstein and Andy Beattie (Macquarie
University), Greg Holwell (University of Auckland) and
anonymous referees for their constructive, thorough, and
thoughtful comments on the manuscript. Colin Bower
(FloraSearch), Graham Brown (Museum and Art Gallery
of the Northern Territory), Stephen Hopper (Royal
Botanic Gardens, Kew) and Eric Scanlen and Ian St
George (New Zealand Native Orchid Group) provided
advice and unpublished data for the list of pollinators in
Table 1. Most photographs were kindly provided by Julia
Cooke (Macquarie University). This work was generously
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