Documente Academic
Documente Profesional
Documente Cultură
LABORATORIUM BIOFARMAKA
FAKULTAS FARMASI
UNIVERSITAS HASANUDIN
Analysis Report
SOP document
Validated
Description :
Design
Analysis
Created/used by
Current user
camag
camag
Stationary phase
Executed by
Plate size (X x Y)
Material
Manufacturer
Batch
GLP code
Pre-washing
Modification
camag
Saturday, January 01, 2005 12:44:08 AM
5.0 x 10.0 cm
HPTLC plates silica gel 60 F 254
E. MERCK KGaA
No
No
Definitions - Screening
Executed by
camag
Samples
awal
Heksan
BJA
Substance name
spot 1
spot 2
Rf
Window size
0.94
0.75
0.500
0.500
Manufacturer
Batch
number
Expiry date
Product
number
11.5 mm
94.0 mm
Instrument
Executed by
Number of tracks
Position of first track X
Distance between tracks
Scan start pos. Y
Scan end pos. Y
Slit dimensions
Optimize optical system
Scanning speed:
Data resolution:
User : camag
Saturday, January 01, 2005 12:50:43 AM
Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215
SN 1410W024, V1.4.3
Page 1 of 5
254
D2 & W
Remission
Absorption
Second order
Automatic
334 V
Detector properties
Y-position for 0 adjust
Track # for 0 adjust
Analog Offset
Sensitivity
9.0 mm
0
10%
Automatic (29)
Integration
Properties
Data filtering
Baseline correction
Peak threshold min. slope
Peak threshold min. height
Peak threshold min. area
Peak threshold max. height
Track start position
Track end position
Display scaling
Savitsky-Golay 7
Lowest Slope
5
10 AU
50
990 AU
11.5 mm
94.0 mm
Automatic
500
[ AU ]
400
350
300
250
200
150
50
100
[ mm ]
50
40
35
0.00
30
0.20
25
20
0.40
15
0.60
10
[ Rf ]
5
1.00 0
User : camag
Saturday, January 01, 2005 12:50:43 AM
Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215
SN 1410W024, V1.4.3
Page 2 of 5
500
450
450
400
400
350
350
300
300
250
250
200
200
150
150
100
100
50
50
0
-0.14
0.06
Start
Peak Rf
1
0.71
2
0.73
3
0.87
0.26
Start
Height
3.6
11.2
0.9
0.46
Max
Rf
0.72
0.75
0.93
Max
Height
13.5
26.5
166.3
0.66
Max
%
6.56
12.84
80.60
0.86
End
Rf
0.73
0.78
0.98
1.06
End
Height
10.4
3.9
17.5
spot 1 @ 254 nm
0
-0.14
0.06
Area
%
1.58
8.35
90.06
Area
131.3
692.3
7465.3
0.26
0.46
0.66
spot 2 @ 254 nm
0.86
1.06
Assigned substance
unknown *
spot 2
spot 1
Track 2, ID:
400
400
350
350
300
300
250
250
200
200
150
150
100
100
50
50
1spot 1 @ 254 nm
0
-0.14
0.06
Start
Peak Rf
1
0.86
2
0.93
0.26
Start
Height
6.7
129.9
0.46
Max
Rf
0.92
0.94
Max
Height
131.1
132.2
0.66
Max
%
49.80
50.20
0.86
End
Rf
0.93
0.99
1.06
End
Height
130.0
20.0
0
-0.14
0.06
Area
%
44.07
55.93
Area
3405.1
4322.0
0.26
0.46
0.66
0.86
1.06
Assigned substance
unknown *
spot 1
Track 3, ID:
400
400
350
350
300
300
250
250
200
200
150
150
100
100
50
50
0
-0.14
0.06
Start
Peak Rf
1
0.85
0.26
Start
Height
0.4
0.46
Max
Rf
0.94
0.66
Max
Max
Height
%
111.5 100.00
User : camag
Saturday, January 01, 2005 12:50:43 AM
0.86
End
Rf
0.96
1.06
End
Height
61.8
spot 1 @ 254 nm
0
-0.14
Area
6625.4
0.06
Area
%
100.00
Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215
0.26
0.46
0.66
0.86
1.06
Assigned substance
spot 1
SN 1410W024, V1.4.3
Page 3 of 5
camag
Saturday, January 01, 2005 12:50:21 AM
All detected peaks
6.00 x 0.30 mm, Micro
Resolution
100 nm/s
1 nm/step
11.5 mm
9.0 mm
Measurement Table
Lamp
Start wavelength
End wavelength
Measurement type
Measurement Mode
Optical filter
Detector Mode
D2 & W
200 nm
700 nm
Remission
Absorption
Second order
Automatic
Detector properties
Y-position for 0 adjust
Track # for 0 adjust
Analog Offset
Sensitivity
0.0 mm
0
10%
Automatic (25)
100.0
[ AU ]
[ AU ]
80.0
80.0
70.0
70.0
60.0
60.0
50.0
50.0
40.0
40.0
30.0
30.0
20.0
20.0
10.0
10.0
0.0
0.0
200.0 250.0 300.0 350.0 400.0 450.0 500.0 550.0 600.0 [ nm ] 700.0
T
1
2
3
Rf
0.93 Rf
0.94 Rf
0.94 Rf
Substance
spot 1
spot 1
spot 1
Max. @
200 nm
200 nm
200 nm
100.0
[ AU ]
[ AU ]
80.0
80.0
70.0
70.0
60.0
60.0
50.0
50.0
40.0
40.0
30.0
30.0
20.0
20.0
10.0
10.0
0.0
0.0
200.0 250.0 300.0 350.0 400.0 450.0 500.0 550.0 600.0 [ nm ] 700.0
T
1
Rf
Substance
0.75 Rf spot 2
User : camag
Saturday, January 01, 2005 12:50:43 AM
Max. @
200 nm
Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215
SN 1410W024, V1.4.3
Page 4 of 5
Track type
Sample
Sample
Sample
Vial
1
1
1
Sample ID
Table of substances
Substance
spot 1
spot 2
Position
MD mm
88.7
73.2
Tracks
1 2 3
A A A
A
User : camag
Saturday, January 01, 2005 12:50:43 AM
Approved :. . . . . . . . . . . . . . . . . . .
Report ID : 07D5010107003215
SN 1410W024, V1.4.3
Page 5 of 5