Biology

240 Lecture Topics, F14

Fundamentals of Microbiology
Biology 240, Fall 2014
Dr. Josh D. Neufeld

This listing includes a tentative outline of Topics covered in the F14 version of Biol 240,
and each topic is preceded by the relevant sections in Wessner et al. (1st ed). The
introductory paragraphs preceding the textbook sections do not appear in this listing,
but you should read them as well! The exact timing of in-class videos is unknown this
year, due to the new textbook. If you are using the 13th edition of Madigan &
Martinko, please see the sections listed in parentheses.

Topic 1: The Microbial World
1.1 The Microbes (1.1, 1.2)
1.2 Microbial Genetics (1.4)
1.3 Microbial Metabolism and Ecology (1.3)
1.4 Microbes and Disease (1.5)

Topic 2: Bacteria
2.1 Morphology of Bacterial Cells (3.1, 3.2)
2.2 The Cytoplasm (3.10, 3.11)
2.3 The Bacterial Cytoskeleton
2.4 The Cell Envelope (3.3 - 3.7)
2.5 The Bacterial Cell Surface (3.9, 3.13, 3.15)
2.6 Bacterial Taxonomy (16.13)

Topic 3: Archaea
4.1 Distinct Properties of Archaea
4.2 Archaeal Cell Structure (3.1, 3.2, 3.3, 3.8, 3.13)
4.3 Diversity of Archaea (2.10, 19.1)

Topic 4: Eukaryal Microbes
3.1 The Morphology of Typical Eukaryal Cells (20.1 - 20.3)
3.2 Diversity of Eukaryal Microbes (20.6)
3.3 Replication of Eukaryal Microbes (7.6)
3.4 The Origin of Eukaryal Cells (16.4, 20.4)
3.5 Interactions Between Eukaryal Microbes and Animals, Plants, and the Environment

Topic 5: Viruses
5.1 A Basic Overview of Viruses (9.1)
5.2 Origins of Viruses
5.3 Cultivation, Purification and Quantification of Viruses (9.4)
5.4 Diversity of Viruses (9.8 9.12)
5.5 Virus-Like Particles (9.13 - 9.15)
5.6 Virology Today

Fundamentals of Microbiology
Biology 240, Fall 2014
Dr. Josh D. Neufeld

Topic 6: Cultivating Microorganisms

6.1 Nutritional Requirements of Microorganisms (4.1)
6.2 Factors Affecting Microbial Growth (5.12 - 5.18)
6.3 Growing Microorganisms in the Laboratory (4.2, 4.3)
6.4 Measuring Microbial Population Growth (5.5 - 5.11)
6.5 Eliminating Microbes and Preventing Their Growth (26.1 - 26.9)

Topic 7: Bacterial MVPs (not covered in Wessner)
Proteobacteria
Purple sulfur bacteria
C1-eaters
Nitrifiers
Pseudomonads
N2-fixers
Enterics
Deltaproteobacteria
Firmicutes (G+ve, no spores)
Firmicutes (G+ve, spores)
Actinobacteria (G+ve, high GC)
Cyanobacteria

Topic 8: Regulation of Gene Expression
11.1 Differential Gene Expression (8.1-8.5, 8.7)
11.2 The Operon (8.6)
11.3 Global Gene Regulation (8.5, 8.11)
11.4 Post-Initiation of Gene Expression (8.14-8.16)
11.5 Quorum Sensing (8.9)
11.6 Two-Component Regulatory Systems (8.7)
11.7 Chemotaxis (8.8)

Topic 9: Bacterial Genetic Analysis
9.1 Bacteria as Subjects of Genetic Research
9.2 Mutants, Mutations, and Strains (10.1-10.5)
9.3 Restriction Enzymes, Vectors, and Cloning (11.1-11.10)
9.4 Recombination and DNA Transfer (10.6-10.13)

Topic 10: Microbial Genomics
10.1 Genome Sequencing (12.2, 12.3)
10.2 Genomic Analysis of Gene Expression (12.7, 12.8)
10.3 Comparative Genomics (12.10, 12.11)
10.4 Metagenomics (12.6)