AE-COE Form 1 - Project Proposal CvSU CVMBS

AE-COE Form 1 Project Proposal Form
CHED CENTER OF EXCELLENCE/DEVELOPMENT

(3rd Batch)
1.
Basic Information
Name of School: CAVITE STATE UNIVERSITY

Address: Brgy. Bancod, Indang, Cavite
COE or COD in: Veterinary Medicine
President
Name : Dr. Divinia C. Chavez
Telephone Number/s: (046) 415 0010
Fax Number/s: (046) 415 0013
Email Address: esancchavez@yahoo.com
Dean
Name: Dr. Rezin C. Bahia
Telephone Number/s:
Office: (046) 862 0939
Mobile: 0917 5047747
Fax Number/s: (046) 862 0939
Email Address: dokrezin@yahoo.com
Center Project Coordinator
Name of: Dr. Alvin-William A. Alvarez
Telephone Number/s:
Office: (046) 862 0939
Mobile: 0923 2045572
Fax Number/s: (046) 862 0939
Email Address: vinalkups@yahoo.com
Cutting Edge Technology/Science (CET/S)

Title: Immunohistochemistry and Microscopy
Brief background on the CET/S:
The CvSU College of Veterinary Medicine and Biomedical Sciences (CVMBS) has
been working towards excellence in the field of veterinary education in the country. The
College has been awarded Level 2 accreditation by the Accrediting Agency of Chartered
Colleges and Universities of the Philippines (AACCUP) for the period of December 16,
2012 to December 15, 2016 and Center of Development (COD) in Veterinary Medicine by
the Commission of Higher Education (CHED) for the period of February 27, 2009 to
February 27, 2012. With these developments, the College has become more committed to
respond to the ever changing needs of the country, particularly in field of veterinary
education and research.
In 2009, the College has acquired equipment to establish an Immunohistochemistry
(IHC) and Microscopy Laboratory that will cater to veterinary researches, as well as
biomedical studies. Unfortunately, the personnel previously trained for this type of
laboratory is no longer connected with the University. Continuous technical trainings on
immunostaining and microscopy were needed to fully operationalize the laboratory which
is why the College conducted a short course on Immunohistochemistry for the Faculty and
staff of the which was facilitated by experts from the College of Veterinary Medicine at
University of the Philippines, Los Baos. And just recently, a faculty member attended an
Immunohistochemistry and Microscopy Course at the Marine Biological Laboratory at
Woods Hole, Massachusetts, USA. With these, the utilization of the laboratory for
research, and even for instruction, may be maximized.
To date, the laboratory has equipment which approximately costs around
7,000,000. These include: (1) automated rotary tissue processor; (2) tissue embedding
console, (3) rotary microtome, (4) Nikon Eclipse 90i state-of-the-art microscope for bright
field and fluorescence microscopy, (4) immunohistochemistry station, (5) ultra low
biological freezer, and (6) ELISA reader. However, to further enhance the capability and
proficiency of the laboratory in the application of the IHC technique to a broader scope,
additional equipment, materials and reagents are needed to be purchased.
Immunohistochemistry or IHC is widely used in basic research to understand the
distribution and localization of biomarkers and differentially expressed proteins in different
parts of a biological tissue using the process of detecting antigens (e.g., proteins) in cells
2
of a tissue section by exploiting the principle of antibodies binding specifically to antigens.

Clinically, immunohistochemical staining is widely used in the diagnosis of abnormal cells
such as those found in cancerous tumors and maybe used to detect antigens of
pathogens in tissue such as rabies in the brain (IFAT). IHC has certainly evolved over the
last few years to become an invaluable technique in many facets of life sciences research
including veterinary medicine.
When using IHC to understand the spatial location of function of a molecule in
tissue, it is important to be confident that the antibodies used bind specifically to their
target epitope. When antibodies are not validated or when IHC protocols are not
optimized, non-specific binding will result in incorrect findings and conclusions. Therefore,
when using this technique, it is critical to take the time to properly validate the specificity of
an antibody and to optimize the protocol in performing a study. When done properly, one
can be confident that the IHC performed is providing the correct information about
changes to the molecule of interest in the tissue.
Western blots, pulse field gel electrophoresis and ELISA are assays similar to IHC
in the sense that they utilize antibody specificity and quantitative changes in the
expression of proteins either in individual cells or biological samples such as tissue
homogenates. If the findings from studies these techniques are in agreement with the
findings of IHC, they allow the investigator to be more confident that the results obtained
are correct.
Similarly, the collection and measurement of mRNA in cells or tissue homogenates
using real-time quantitative polymerase chain reaction (RT-PCR) can confirm findings with
IHC. Effecting similar changes in the mRNA for the target epitopes as measured with IHC
and quantitative microscopy augment the confirmation of the specificity of an antibody.
The cutting edge technology proposal is geared toward building the proficiency of
the IHC laboratory as a tool for veterinary research and diagnosis. It has three
components: (1) basic research, (2) training, and (3) technology development.
Moreover, the IHC technique may not only be used in veterinary research but will
also cater to the research needs of the Cavite State University as the National Coffee
Center of the Philippines. There is growing popularity of Civet Coffee or Kape Alamid in
the Philippines. Researches on Civet Coffee production may boost the profitability and
sustainability of enterprise. Furthermore, it will serve as a boost for the sleeping coffee
industry creating more employment and business opportunities.
2.
Executive Summary
Summary of Cutting-Edge Technology
IMMUNOHISTOCHEMISTRY AND MICROSCOPY PROGRAM
INTRODUCTION
In 2009, the College has acquired equipment to establish an Immunohistochemistry
(IHC) and Microscopy Laboratory that will cater to researches foremost, in the veterinary
field. Continuing technical trainings on immunostaining and microscopy is needed to fully
operationalize
the
laboratory.
Just
recently,
faculty
member
attended
an
Immunohistochemistry and Microscopy Course at the Marine Biological Laboratory at

Woods Hole, Massachusetts, USA. With this development, it is envisioned by the College
to use the expertise learned from the training to maximize the potential of the laboratory
for research. To date, the laboratory has the following equipments: (1) automated rotary
tissue processor; (2) tissue embedding console, (3) rotary microtome, (4) Nikon Eclipse
90i state-of-the-art microscope for bright field and fluorescence microscopy, (4)
immunohistochemistry station, (5) ultra low biological freezer, (6) ELISA reader. However,
there is a need to develop the proficiency of the laboratory in actual running of the IHC
technique. To do this, additional equipments and reagents are needed to be purchased to
maximize the potential of the laboratory. Equipments which incudes (1) RT-PCR, (2) Pulse
Field Gel Electrophoresis and (3) Western blot will be utilized to build proficiency in the
IHC technique.
The cutting edge program has three components: (1) basic research, (2) training
and (3) technology development.
A. Basic Research
Project Title: Immunohistochemistry of the Pancreatic Islets of Selected Wildlife in the
Philippines
Study 1 Title: Immunohistochemistry of the Pancreatic Islets of Selected
Frugivorous and Insectivorous Bats in the Philippines (Order
Chiroptera)
Study 2. Title: Immunohistochemistry of the Pancreatic Islets of Frugivorous and
Carnivorous Monitor Lizard in the Philippines (Genus Varanus)
Objectives:
The general objective of the study is to document, describe and differentiate histological
characteristics of the endocrine pancreas of certain wildlife species using
immunohistochemistry. Specifically, this study will be conducted to identify insulin and
glucagon reactive pancreatic cells and to compare and contrast the pancreatic islet size,
density, location of islet cells of selected species. This study shall investigate the
morphological adaptation of the endocrine pancreas toward diet preferences of some
Philippine wildlife. The description of the morphophysiological features can be used to
deduce ecological and evolutionary implication of body forms.
Expected outputs
The study is expected to provide data on the morphophysiological properties of tissues
from various endemic wildlife. The study is also expected to help increase the proficiency
of CVMBS personnel in doing immunohistochemistry work for research and instruction
purposes. The study also will also give them the chance to publish results the field of
immunohistochemistry and microscopy in prestigious journals and periodicals.
Target beneficiaries
College of Veterinary Medicine and Biomedical Sciences, Cavite State University as it
seeks more research endeavors from its constituents to attain global recognition
INPUT
Projected
Expenses
Year 1
300,000
Year 2
150,000
Year 3
75,000
525,000
B. Training
Project Title: Immunohistochemistry and Microscopy Course
Objectives:
The general objective of the project is to conduct re-echo training to interested individuals,
students and professional alike.
Expected outputs
Trained individuals (students and other professionals) interested in using
immunohistochemistry and microscopy in their research work or work related activities.
Students (undergraduates, masteral, doctoral), post-doctoral, researchers, laboratory
technicians and other interested individuals
INPUT
Projected
Expenses
Year 1
-
Year 2
250,000
Year 3
250,000
500,000
C. Technology development (Research Capability Building)

Project Title: Development of Technologies for Disease Detection in Livestock Using
Immunohistochemistry
Objectives
1. Augment the diagnosis, treatment and control of economically important animal
disease
2. Increase productivity of livestock farmers by definitive diagnosis of animal diseases
using IHC technique
3. Augment the diagnosis, treatment and control of zoonotic diseases
Expected outputs
To generate scientific data on diseases affecting livestock which have economic and
public health importance
Field veterinarians, small scale farmers, commercial farms, researchers, Local
Government (LGU) veterinary and agricultural officers
INPUT
Projected
Expenses
Year 1
800,000
Year 2
400,000
Year 3
200,000
1,400,000
3.
Capsule Three-Year Work and Financial Plan
CHED Center of Excellence/Development in Agriculture Education

Program: Doctor of Veterinary Medicine
Implementing Unit: Cavite State University
Priority Projects
(The proposal should be geared towards developing or improving a
particular cutting edge technology/science)
Work Plan
Year 2
Year 1
Q
1
Q
2
Q
3
Q
4
Q1
Q
2
Q
3
Year 3
Q
4
Q
1
Q
2
Q
3
Year
1
Financial Plan
Year
Year
2
3
Total
Q
4
1.
Seminars, Workshops and Short Courses
250,000
250,000
500,000
2.
Visiting Professors (Local and Foreign)
300,000
300,000
600,000
3.
Instructional Materials Development
4.
Upgrading of Facilities
2,700,000
500,000
500,000
3,700,000
5.
Upgrading of Library Holdings
6.
Research Capability Building Human Resources and Facilities
800,000
400,000
200,000
1,400,000
7.
Innovative Research Projects (Cutting Edge Technology/Science)
300,000
150,000
75,000
525,000
8.
Local Paper Presentations
100,000
100,000
200,000
9.
Support for Local or National Conferences
100,000
100,000
200,000
50,000
50,000
100,000
25,000
25,000
25,000
75,000
114,750
267,750
4,207,500
56,250
131,250
2,062,500
48,000
112,000
1,760,000
219,000
511,000
8,030,000
10. Visiting Research Fellowships

11. Exhibits/fairs, IEC and Commercialization
12. Industry Government - Academic Linkage Projects
13. Assistance to Non-Centers Projects
Office Supplies and Materials (3%)
Common Facilities and Assistance (7%)
Total
CHED Center of Excellence/Development in Agriculture Education (Detailed Yearly Work and Financial Plan)
Year 1
Priority Projects
(Entries in this column should be quantified and specific/detailed. The proposal should be geared
towards developing or improving a particular cutting edge technology/sciences
J F M A M J J A S O N D
1. Seminars, Workshops and Short Courses
1.1. Immunohistochemistry (IHC) and Microscopy Training Course
2. Visiting Professors (Local and Foreign)
3. Instructional Materials Development
4. Upgrading of Facilities
4.1 Purchase of IHC Technique Validating Equipments
4.1.1
Purchase of Real Time - PCR
4.1.2
Purchase of Western Blot
Subtotal
Amount
2,500,000
200,000
2,700,000
5.
6.
800,000
7.
Innovative Research Projects

7.1 Immunohistochemistry of the Pancreatic Islets of Selected Wildlife in the Philippines
300,000
8.
9.

25,000

Total
114,750
267,750
4,207,500

Year 2
Amount
Priority Projects
J
1.

4.1 Purchase of IHC Technique Validating equipment and safety equipment
4.1.1
Biological Safety Cabinet (Level 2)
4.1.2
Pulse field gel electrophoresis
250,000
300,000
Subtotal
390,000
110,000
500,000
5.
6.
400,000
7.

150,000
8.
100,000
9.
100,000

50,000
25,000

56,250
131,250
Total
2,062,500
Year 3
Amount
Priority Projects
J
1.

4.1 Other Equipments for Animal Disease Diagnosis
4.1.1
Rectal Ultrasound Probe
4.1.2
Blood Biochemistry Machine
250,000
300,000
Subtotal
5.
6.
7.

150,000
350,000
500,000
200,000
75,000
8.
100,000
9.
100,000

50,000
25,000

Total
48,000
112,000
1,760,000
CAVITE STATE UNIVERSITY

College of Veterinary Medicine and Biomedical Sciences
IMMUNOHISTOCHEMISTRY AND MICROSCOPY PROGRAM
Priority Projects:
Upgrading of Facilities
Rationale
Improvement of the colleges laboratories shall strengthen instruction by
providing hands-on experience of students and shall enhance its capability to
conduct researches as well as provide diagnostic services to its clientele. In addition,
purchased equipments shall complement evaluation and validation of IHC technique
in the innovative research project of this proposal.
Objectives
a. To upgrade existing instructional laboratories
b. To provide diagnostic services for research and extension purposes
c. To evaluate and validate IHC technique in the innovative research project of this
proposal
C.
Activities (narrative explanation of activities)

a. Purchase equipments for the evaluation and validation of IHC technique in the
innovative research project of this proposal
RT-PCR, Pulse Field Gel Electrophoresis and Western Blot serves as a
tool to evaluate and validate the IHC technique/protocol in the innovative
research project of this proposal
b. Purchase of state-of-the-art equipments for the Upgrade of Animal Disease
Diagnostic Laboratory (ADDL)
ADDL serves as tool for diagnosis. Upgrading includes acquisition of
basic equipments for evaluation blood chemistry profiles of animal patients and
animals of concern for research. RT-PCR and Pulse Field Gel Electrophoresis
are also used as a diagnostic tool for animal diseases.
c. Purchase of equipment for Enhancing the Diagnostic Imaging Laboratory
Additional purchase of rectal ultrasonograph probe shall enhance the
capability of the existing ultrasound machine for obstetrical and
theriogenological purposes.
d. Purchase of Biological Safety Cabinet
Handling of certain materials for research and diagnostic may pose
occupational and environmental hazard. Purchase of a biological safety cabinet
will minimize such dangers.
D.
Expected Deliverables
a. State-of-the-art laboratories
b. Research output related to immunohistochemistry and microscopy
c. Research output related to other fields in veterinary medicine like
microbiology
d. More knowledgeable graduates
2. Research Capability BuildingHuman Resources and Facilities (Technology Development)

Technology Development for Animal Disease Diagnosis using IHC needs purchase of
other reagents, materials, and miscellaneous equipments (i.e liquid nitrogen storage tank,
pippetors). Likewise, upgrades of existing state-of-the-art microscope (i.e. microscope
objectives and excitation filters), purchase of latest specification computers and quantitative
microscopy softwares are needed to support the research capability for the technology
development component of this proposal.
Innovative Research Projects (please see attached DOST Format Proposal)
Project Title: Immunohistochemistry of the Pancreatic Islets of Selected Wildlife in the
Philippines
Study 1 Title: Immunohistochemistry of the Pancreatic Islets of Selected
Frugivorous and Insectivorous Bats in the Philippines (Order
Chiroptera)
Study 2. Title: Immunohistochemistry of the Pancreatic Islets of Frugivorous and
Carnivorous Monitor Lizard in the Philippines (Genus Varanus)
Objectives:
The general objective of the study is to describe and differentiate histological
characteristics of the endocrine pancreas of certain wildlife using immunohistochemistry.
Specifically, this study will be conducted to identify insulin and glucagon reactive
pancreatic cells and to compare and contrast the pancreatic islet size, density, location of
islet cells of selected species. This study shall investigate the morphological adaptation of
the endocrine pancreas toward diet preference of some Philippine wildlife. The description
of the morphophysiological features can be used to deduce ecological and evolutionary
implication of body forms.
Expected outputs
The study is expected to increase proficiency of CVMBS personnel in doing
immunohistochemistry work for research and instruction purposes. The study also will
also give CVMBS personned the chance to publish his research work on the field of
immunohistochemistry and microscopy.
A. Project Title: Immunohistochemistry and Microscopy Training Course

B. Objectives:
The general objective of the project is to conduct re-echo training to interested
individuals, students and professional alike.
C. Activities (narrative explanation of activities)
a. conduct of a 1-week training course to train individuals (students and other
professionals) interested in using immunohistochemistry and microscopy in their
research work or work related activities.
D. Expected Deliverables
a. Target beneficiaries such as students (undergraduates, masteral, doctoral), post
doctoral, researchers, laboratory technicians and other interested individuals
will be trained to do IHC for research and instructional purposes
4. Visiting Professors
IHC and microscopy experts from abroad will be invited. They can serve as resource
persons and research collaborators.
5. Local Paper Presentation
Research generated will be presented in various fora. Funding support for paper
presentation will encourage researchers involved in the project to present their works in
various scientific fora.
6. Local and National Conferences
Continuing education and establishment of future collaboration are some of the
outcomes of attending local and national conferences. Funding support will encourage faculty
members to attend relevant conferences to gain up-to-date information and establish
partnerships for research and development activities.
IEC materials and activities with regards to IHC and microscopy is important in the
promotion and dissemination of the technology.
Establish linkages to industry for support for the innovative research project.
Partnership with industry stakeholders may be a possible source of extramural funding for the
sustained development and utilization of the technology.

AE-COE Form 1 - Project Proposal CvSU CVMBS

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CHED CENTER OF EXCELLENCE/DEVELOPMENT

Name of School: CAVITE STATE UNIVERSITY

Cutting Edge Technology/Science (CET/S)

of a tissue section by exploiting the principle of antibodies binding specifically to antigens.

Immunohistochemistry and Microscopy Course at the Marine Biological Laboratory at

C. Technology development (Research Capability Building)

Capsule Three-Year Work and Financial Plan

CHED Center of Excellence/Development in Agriculture Education

Seminars, Workshops and Short Courses

Visiting Professors (Local and Foreign)

Instructional Materials Development

Upgrading of Library Holdings

Research Capability Building Human Resources and Facilities

Innovative Research Projects (Cutting Edge Technology/Science)

Local Paper Presentations

Support for Local or National Conferences

10. Visiting Research Fellowships

Upgrading of Library Holdings

Research Capability Building Human Resources and Facilities

Innovative Research Projects

Local Paper Presentations

Support for Local or National Conferences

10. Visiting Research Fellowships

13. Assistance to Non-Centers Projects

CHED Center of Excellence/Development in Agriculture Education

Seminars, Workshops and Short Courses

Upgrading of Library Holdings

Research Capability Building Human Resources and Facilities

Innovative Research Projects

Local Paper Presentations

Support for Local or National Conferences

10. Visiting Research Fellowships

12. Industry Government - Academic Linkage Projects

13. Assistance to Non-Centers Projects

Seminars, Workshops and Short Courses

Upgrading of Library Holdings

Research Capability Building Human Resources and Facilities

Innovative Research Projects

Local Paper Presentations

Support for Local or National Conferences

10. Visiting Research Fellowships

12. Industry Government - Academic Linkage Projects

13. Assistance to Non-Centers Projects

CAVITE STATE UNIVERSITY

Activities (narrative explanation of activities)

2. Research Capability BuildingHuman Resources and Facilities (Technology Development)

A. Project Title: Immunohistochemistry and Microscopy Training Course

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