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repair surgically
Hormonal mainly prolactinemia and testosterone deficiency
genital
Mullerian duct absent, but present derivated wolfian
Samples management
Endocrine analysis
Reference
techniques. Proc final research coordination meeting, join FAO-IAEA vieena Austria
Mahaputra l, dan K. suhadi 1993. Kadar estosteronurin pagi dan siang haripada pria menikah
dan belum. Media IDI. 18
Preparing samples
Plasma
Serum
Sperm
Urin
faeces
Product cells
Milk
The others
Fruits
leafs
Organs
Hormonal quantitative analysis (RIA, IRMA, ELISA)
Blood plasma/serum/milk/semen
Product cells, Organ bodyfluid, Feaces, Urine,All species including plant or fruit
Blood plasma/serum
Milk sample
As well as known individually, breed and species having a lot variation fat content in milk
Event in the same reproductive phase in order have to behormone concentration almost the
same, but found much variation is due to cetainsoluble hormone in cream
Semen sample
Sperm evaluation
Product cells
commencement
faeces sample
homogenized I,0g normal faeces consistency with 1 ml NAcl physiol. >>>> centrifuge 1000Xg
15
pick up supernatant and add with petroleum benzene with proportion 1:5
vortex for 3MNM
put into freezer for 15
the pellet freezing at bottom
paw it out filtrate into assay tube
evaporation the filtrate by using waterbath 38 0c and air blowing pump into each hole surface of
if the faeces too solid, increase the dilution in proportion 1G:5ml during homogenizing sample
in the calculation, the result should be multiply by 5
to monitoring of cycle
progesterone depend on species
twice a weeks for duration twice cycle
in cows 2x21 D
primate and women 28X2 D
to monitoring ovulation
Following treatment the sample should be withdrawn at least every 30, much better in 20
interval
Therefore the minimal and maximal fluctuation concentration can be differentiated
Let say GnRH against LH or FSH
Because gradually the concentration low at noon and high early morning time
Most of adult man will be felt and occur erection in early morning much frequent than at noon
At morning milking time always collected more quantitatively milk than at noon milking time
Physiologically this hormone will be decrease in order of postpartum period
Over lower than cut off point concentration will be releasing of gonadotropin
Effect positive felt by dairy farmer because arise this hormone mainly in early morning effected
In couple just now marrie this increasing to be effect on sperm production. High concentration
in long time to be depress of gonadotropin hormone well as FSH and LH >>> infertile
Contrary for fertile male. By increasing of prolactin as a technique of contraceptic
Morningafternoon
Bachelor
350 ng/dl
360ng/dl
(20-27 y)
Married 265ng/dl
206ng/dl
(25-35y)
Mahaputra &soehadi (madia IDI 1993)