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1913
Reuben Ottenberg: stressed importance
of compatibility testing
1914
Albert Hustin: sodium citrate as an
anticoagulant solution
1915
Richard Lewisohn: minimum amount of
citrate needed for anticoagulation
1916
Rous and Turner: introduction of citrate
dextrose solution for RBC preservation
1924
Felix Bernstein: proof of inheritance of
blood groups
Issues in race distribution
1927
American Association of Immunologists:
adopted the current ABO terminology
proposed by Landsteiner
1939-1940
Philip Levine (together with Stetson,
Landsteiner and Alex Wiener): 1st
discovery of Rh blood groups
1941
Charles Drew: developing techniques in
blood transfusion and blood
preservation during WWII
1943
Loutit and Mollison: acid-citrate dextrose
formula
1945
Robin Coombs, Rob Race and Arthur
Mourant: (re)discovery of anti-human
globulin (AHG) sera and antiglobulin test
which was first described by Carlo
Moreschi in 1908
1947
Rh immune Globulin for prevention of
Hemolytic Disease of the Fetus and
Newborn
1951
Edwin Cohn: development of cell
separator, paved the way for component
therapy
Carl Walter: blood collection using a
collapsible bag of polyvinyl resin
1957
Gibson: introduction of citratephosphate-dextrose (CPD)
1965
Judith Pool: concentrated factor VIII
found in the cryoprecipitate portion of
plasma
1968
Brinkhous and Shanbrom: pooling of
plasma units to produce factor VIII
concentration
Adverse effects of Transfusion
1970s
Mainly ANAEROBIC
1.
2.
3.
4.
Hemoglobin function
Oxygen delivery to tissues
- 2-3 DPG
- Tense form lower High affinity to
oxygen
- Relaxed form higher High affinity
to oxygen
Additive Solutions
Preserving solutions that are added to
the RBCs after removal of plasma
with/without platelets
Beutler development
Lovric and Hogman implementation
a. Lovric CP2D and additive solution
(saline, adenine, glucose, trisodium
citrate, citric acid, sodium
phosphate)
b. Hogman CPD and additive
solution (saline, adenine, glucose
(SAG), and later with mannitol
(SAGM)
Ligands
1. H+ ions
2. CO2
3. Organic phosphates (2,3 DPG)
Shift to the right
- Increased 2,3 DPG = decrease High
affinity to oxygen = increase oxygen
delivery to tissues
RBC Preservation
RBC viability
Measure of in-vivo RBC survival
following transfusion
75% of cells transfused should remain
viable for 24 hours
Liquid state at 1-6 degrees Celsius for a
specific number of days
RBC freezing
- For autologous units and storage of
rare blood types
- -65 C, 10 years
- Glycerol
Storage lesion
RBC Rejuvenation
PIPA
Rejuvesol used to salvage liquidstored RBCs that have reached outdate