Protein Engineering and Proteomics

Version No.
Prerequisites
Objectives:

LTPC: 3 0 0 3

1.0.0
Biochemistry, Molecular biology and Genetic Engineering
To make the student familiarize with the basics, concepts and application of
protein engineering and proteomics
Expected Outcome:
Explain the principles involved in the maintenance of protein structure
Analyze the given protein structure and predict the sites to be engineered for
altering/introducing a specific property
Apply the concepts of proteomics for novel applications
Unit No. 1
Chemical Properties and Evolution of Proteins
Number of hours:
12
Amino acid structure and properties- Detection of the sizes of proteins covalent structures in proteins
overview of chemical and biosynthesis of proteins topogenesis post translational covalent modification of
polypeptide chains. Evolutionary divergence Reconstructing evolution from contemporary sequences gene
rearrangements and the evolution of protein complexity genetics to probe protein structure physical nature of
non-covalent interaction interaction(s) with solvent hydrophobic interaction intramolecular interactions
degradation of proteins.
Unit No. 2
Conformation of Proteins
No. hours:
10
Overview of the levels of structures in globular proteins Ramachandran plot X-ray and NMR measurements
for the determination of three dimensional structures of proteins theoretical structure prediction methods
flexibility and stability of protein structure mechanism of protein folding physical and chemical properties of
soluble and membrane proteins
Unit No. 3
Rational Engineering and Design of proteins
No. hours:
11
Introduction to protein engineering site directed and random mutagenesis stability enhancement
bacteriophage display libraries design of metalloproteins engineering therapeutic antibodies enhanced
recovery and folding of recombinant proteins using fusion protein strategies protein engineering for affinity
purification biosensor and vaccine development
Unit No. 4
Proteomics
No. hours:
12
Analytical protein and peptide separations protein digestion techniques MS for protein and peptide analysis
protein identification by peptide mass finger printing tandem mass and coupled techniques. Application of
proteomics: mining proteomes protein expression profiling protein-protein interactions mapping protein
modifications.
Text Books
1. Creighton TE (2002) Proteins: Structure and Molecular Properties, WH Freeman and
Company. (Modules 1 & 2)
2. Cleland JL & Craik CS (Ed) (1996) Protein Engineering Principles and Practice, John Wiley
& Sons. (Module 3)
3. Lilia Alberghina (2003) Protein Engineering in Industrial Biotechnology, Harward academic
Publishers. (Module 3)
4. Daniel C Liebler (2002) Introduction to Proteomics, Humana Press. (Module 4)
References
1. David Whitford (2005) Proteins: Structure and function, John Wiley and Sons.
2. Mahmoud Hamdan & Pier Giorgio Righetti (2005) Proteomics Today, John Wiley & Sons.
3. Reiner Westermeir and Tom Naven (2002) Proteomics in Practice: A Laboratory manual of
Proteome Analysis, Wiley VCH.
5. Alian Bernot (2004) Genome Transcriptome and Proteome Analysis, John Wiley and Sons.
Mode of Evaluation
Written Examinations/ Assignments/ Seminars
Recommended by the Board of Studies on
13th November, 2008
Date of Approval by the Academic Council
25th November, 2008

Proceedings of the 16th Academic Council held on 25.11.2008