Documente Academic
Documente Profesional
Documente Cultură
101-110, 2012 ISSN 1540-7535 print, Copyright 2012 by New Century Health Publishers, LLC
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All rights of reproduction in any form reserved
Milk,
Nutraceuticals,
et al., 1988; Luck et al., 1994). -1,2,3,4,6-penta-O-Galloyld-glucose binds strongest to the caseins (-casein>s1-casein>Kcasein), followed by -lactalbumin, -lactoglobulin and blood
serum albumin (Luck et al., 1994). The extent of proteinPC interactions is also dependent on pH (interaction is
maximal at the isoelectric point of the protein) and the
molecular properties of the PC (i.e., size, conformational
flexibility and solubility). These properties can be
manipulated to modulate the strength of complexation, i.e.,
addition of each galloyl group (di-tri- tetra-penta) to
galloyl-d- glucose enhances the efficacy of association with
proteins (Spencer et al., 1988). The chemical reactivity of
PCs in protein-polysaccharide- PC systems has also been
demonstrated. Carrageenans (k and i) and other
polysaccharides markedly inhibit the ability of polyphenols
to precipitate sodium caseinate. The mechanism of inhibition
is not fully understood, although encapsulation of PCs by
polysaccharides and/or an increase in the solubility of
protein PC complexes through the formation of a tertiary
complex with polysaccharides has been suggested (Luck et
al., 1994). ProteinpolysaccharidePC interactions can be
regulated through the judicious selection of the
polysaccharide.
PCs undergo enzymatic, thermal or
alkaline-catalysed oxidation to quinone which interact with
reactive amino acid residues such as lysine, tryptophan,
methionine, histidine, tyrosine and cysteine and enhances
oxidative gelation.
Fortification
of
food
products with herbs
and
dairy
(polyphe
nol)
The addition of catechins at 1002000 mg/kg to
bifidobacteria-containing yoghurt has been reported to
improve the survival of the bifidobacteria during storage
(Akahoshi and Takahashi 1996). Currently, polyphenol rich
extracts from green tea (catechins) are being incorporated to
fruit flavored milk drinks as well as other products such as
chewing gum and sweet paste biscuits (Anonymous 1977). It
has also been reported that the addition of aloe vera, which
has a high concentration of aloin and other phenolic
compounds, to yoghurt increases the survival of
bifidobacteria (Pszczola,
1998). The production of soy-whey protein based yoghurt
using an isoflavone-enriched soy protein preparation (2.5
mg isoflavone/g) has been patented and it is claimed that
in addition to providing a source of dietary isoflavones, the
resulting yoghurt had superior organoleptic properties (Crank
and Kerr, 1999). The addition of a polyphenol-rich wine
extract to milk and yoghurt products, as a nutritional
additive, has also been patented (Howard et al., 2000).
Indian herbs such as turmeric (Curcuma longa L.),
coriander (Coriandrum sativum L.), curry leaf
(Murraya koenigii L.), spinach (Spinacia oleracea)
and aonla (Emblica officinalis) are good sources of
antioxidants. These herbs, as a paste, were incorporated
separately at the 10% level into sandesh (a heat-desiccated
product of coagulated milk protein mass or chhana, which is
analogous to cottage cheese). Their total antioxidative status
was assessed in vitro using Randoxs method. The
antioxidative levels of the various herbs were compared with
the synthetic
Sensory
qualities
Generally flavonoids have been attributed to have two types
of flavors for example, bitter and astringent. Astringency is
described sensorily as a puckering, rough or drying mouth
feel. The chemical compounds present in herbs usually form
complexes with salivary protein (mol wt.500-3000 D)
resulting in formation of compounds responsible for
astringency (Bate- Smith 1962). However, the exact
phenolic compound and mechanisms through which bitter
taste perception occurs are not well understood (Kinnamon
et al., 1992).
INFLUENCE OF HERB
HEALTH ATTRIBUTES
COMPONENTS
ON
Immunomodulatory
activity
Herbal drugs are known to possess immunomodulatory
properties and generally act by stimulating both specific and
non-specific immunity (Wagner et al., 1985). Many plants
used in traditional medicine have immunomodulating
activities. Some of these stimulate both humoral and cell
mediated immunity, while others activate only the cellular
components of the immune system, i.e., the phagocytic
function, without affecting the humoral or cell mediated
immunity (Atal et al., 1986). About 34 plants are
identified by rasayanas in the Indian Ayurvedic system of
medicine having various pharmacological properties such
as immunostimulant, tonic, neurostimulant, antiageing,
antibacterial, antirheumatic, anticancer, adoptogenic and
antistress (Agarwal et al., 1999). Herb (Pueraria
tuberosa) added milk showed that there was significant
increase (P<0.05) in immunoglobulins (lgA and lgG)
concentration and % phagocytes while there was a non-
Antioxidant
activity
Antioxidant activity plays an important role in
pharmacological activities such as anti-aging, antiinflammatory, anti-atherosclerosis, and anti-cancer activities
(Lee et al., 1998; Middleton et al., 2000). Inhibition of
free radical induced damage by supplementation of
antioxidants has become an attractive therapeutic strategy
for reducing the risk of diseases (Brash and Harve,
2002). Several synthetic antioxidants are available, but are
quite unsafe and considerably toxic (Madhavi et al., 1995).
Natural products with antioxidant activity may be used for
human consumption because of their safety. Aqueous extract
of Terminalia chebula was tested for potential
antioxidant activity by examining its ability to inhibit radiation-induced lipid peroxidation in rat liver microsomes
and damage to superoxide dismutase enzyme in rat liver
mitochondria (Anita 2000). Verma et al., (2009) studied
effect of crude powder of Indian kudzu on different
animals by feeding at different intervals and found
reduced frequency of angina event and myocardial oxygen
consumption (unstable angina patient), inhibition of lipid
peroxidation in liver, kidney (mice) and brain (rabbit) as
well as enhanced activity of superoxide dismutase in animal
blood and brain in dose dependent relationship. Wiseman
(1996a) suggests that dietary isoflavones genistein and
daidzein have a potential to reduce the risk for
cardiovascular
disease by inhibiting LDL oxidation.
Isoflavones have a chemical structure similar to estrogen,
which was reported to have a weak antioxidant activity because
of the hydroxyl group on its A ring which is in the same
location as in vitamin E (Tiidus 1995), leading to the
Optimized extraction
medium
Herbs are rich in number of chemicals. To claim healthy
benefits, the proper solvent should be screened. The
composition of herbal constituents may vary in different
extraction solvents. The mode of herb addition is very
important aspect. The herbal extract is more beneficial to add
instead of whole plant components, but this may increase the
product cost.
Process
stability
Certain herbal preparations require different processing
which may affect the stability of herbs during fortification.
For example, while preparing herbal ghee the most of
components may disappear at the time of high
temperature processing. So, selected herb should be
stable to different processing treatments.
Product
Stability
Herbs and milk have different shelf-life. Milk and milk
products have very less shelf-life. The fortification of herbs
may affect the shelf-life of both the components. The
antioxidants present in the herbs may enhance the shelflife of herbal products. The product must be stable after
fortification.
Geographical
Variation
The extent of beneficial effects of herbs may vary with the
geographical variations.
CONCLUSIO
NS
Herbs and nutraceuticals are natural substances with
wide range of health attributes. Milk has always been a
choice of innovation for food researchers to meet the ever
changing consumers preferences for newness in the products.
Moreover, milk is consumed by people of all age can act as
potent carrier for the herbs which can add functional
attributes to the product and consumers well being.
However, there are several technological challenges that have
to be overcome to develop milk products enriched with
herbs.
REFERENC
ES
Afolayan, A. J., and Meyer, J. J. M. (1997). The
antimicrobial
activity of 3, 5, and 7- tri hydroxyl flavone isolated from the
shoots of Helichrysum aureonitens. Journal of
Ethnopharmacology
57:177
181.
Agarwal, R., and Singh H. R., (1999). Studies on
immunomodulatory activity of Withania somnifera
in
of
experimental immune
Ethnopharmacology
forages.
Journal
of