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Abstract
To assess drought tolerance, an understanding of sesame (Sesamum indicum L.) physiological properties is needed. The objective
of this study was to determine the physiological responses of sesame accessions to drought stress. The experiment was conducted
as a two-way factorial arranged in a randomized complete block design with three replications. All of the measured traits were
accession- and drought-level-specific and discriminated the accessions into tolerant (Isfahan4, Rashtkhar, and Borazjan),
moderately tolerant (Isfahan1, Ahvaz, Shiraz, Ardestan, and Kal), and sensitive (Markezi, Ultan, Khaf2, Khaf1, and Shahreza)
groups. The results also showed that Khaf2 and Isfahan4 were the best accessions under normal and drought conditions,
respectively. In addition, based on the correlation coefficient of the traits with seed yield, root proline and leaf carotenoid
content were the superior markers with regard to drought tolerance in sesame. This study showed that accessions differences in
drought tolerance could be attributed to the ability of sesame plants to accumulate metabolites under drought conditions, and
these metabolites could be a useful tool for depicting water-deficit tolerance of sesame genotypes, which could be useful to plant
breeders for selecting and developing drought-tolerant cultivars.
A g ro n o my J o u r n a l Vo l u m e 10 6 , I s s u e 1 2 014
111
SSI =
1-Y si Y pi
SI = 1-
SI
Ys
Yp
Error
76
35.41
400.4
1.4
7.9
0.16
0.01
0.22
0.001
0.19
Table 2. Effects of drought level (D), genotype (G), and their interaction on leaf relative water content (RWC) and soluble sugars of sesame under three
levels of drought (I1, I2 , and I3 = 55, 75, and 85% depletion of available soil water).
RWC
Accession
Rashtkhar
Khaf2
Khaf1
Isfahan4
Shahreza
Borazjan
Ahvaz
Kal
Shiraz
Markazi
Ardestan
Ultan
Isfahan1
Avg.
I1
I2
Avg.
%
51.6 cde
66.9 a (+29.5)
72.0 a (+39.4)
63.5 A
42.2 efghi
39.9 fghij (5.5)
27.3 lmn (32)
36.5 E
56.2 bc
37.9 fghijk (32.6) 32.2 jklm (42.6) 42.1 D
21.8 n
28.6 klmn (+31)
31.6 jklm (+45)
27.3 H
42.8 efgh
67.7 a (+58)
34.6 hijkl (19)
48.4C
53.4 bcd
39.9 fghij (25)
62.6 ab (+17)
52.0 B
32.9 ijklm
38.8 fghij (+18)
36.1 ghijkl (+9.5) 35.9E
31.9 jklm
35.6 ghijkl (+12)
30.9 jklmn (3)
32.8 F
39.2 fghij
46.2 def (+18)
42.9 efgh (+9.6) 42.8 D
44.4 defgh
43.2 efgh (2.5)
39.4 fghij (11)
42.3D
37.8 fghijk
46.0 def (+21.6)
42.7efgh (+13)
42.2D
36.7 fghijkl
27.1 lmn (26)
24.6 mn (33)
29.5 G
40.1 fghij
45.2 defg (+13)
43.7 defgh (+9)
43.0 D
40.8B
43.3 A
40.1 B
I1
I2
I3
Avg.
mg g1
137.6 jklmno
199.0 c (+44.6)
179.4 cdef (+30)
172.0 D
197.9 cd
175.7 cdefg (14)
165.2 efghijk (16.5)
179.6 C
146.2 ghijklmno
124.0 no (15)
119.6 o (18)
130.0 I
129.5 mno
182.4 cdef (+41)
166.17defghijk (+28.6)
159.3 E
167.5 cdefghij
141.9 ijklmno (15)
126.8 mno (24)
145.4 FG
174.1 cdefgh
350.0 a (+101)
321.0 a (+84.4)
281.7 A
123.3 o
163.7 efghijkl (+33)
141.5 ijklmno (+15) 142.8 FGH
130.6 mno
156.6 efgn (+20)
125.8 no (3.6)
137.7 H
135.6 jklmno
167.7 cdefghij (+23.6)
135.8 jklmno (0.3)
146.4 F
188.3 cde
164.6 efghijk (12.6)
177.6 cdefg (6)
176.8 CD
131.3 lmno
158.74 efghijklm (+21)
129.1 mno (2)
139.7 GH
152.6 fghijklmno 133.6 klmno (12.4)
130.6 mno (14.4)
138.9 H
174.5 cdefgh
283.3 b (+62.4)
272.0 b (+60)
243.3 B
153.0 C
184.7 A
168.5 B
Means in columns and row (interaction) for each treatment followed by the same lowercase letter(s) are not significantly different at the 5% probability level.
Values in the parentheses are the percentage reduction (-) or increase (+) compared with the control (I1).
Means in each column (main effect of genotype) or row (main effect of drought level) for each treatment followed by the same uppercase letter(s) are not significantly different at the 5% probability level.
113
Table 3. Effects of drought level (D), genotype (G), and their interaction on leaf and root proline contents of sesame under three levels of drought
(I1, I2 , and I3 = 55, 75, and 85% depletion of available soil water).
Leaf proline content
Accession
I1
I2
Avg.
I1
I2
I3
Avg.
g1
Rashtkhar
Khaf2
Khaf1
Isfahan4
Shahreza
Borazjan
Ahvaz
kal
Shiraz
Markazi
Ardestan
Ultan
Isfahan1
Avg.
mmol
1.18 o
2.43 lmno (+105) 5.24 hijk (+346) 2.95 H
10.52 efghijklm
34.72 c (+230)
37.10 c (+252.5)
27.4B
3.89 klm
4.34 kl (+11.4)
4.56 ijk (+17)
4.27 G
16.01 defgh
17.81 def (+11)
12.98 ghijk (19)
15.6C
5.27 hijk
5.09 hijk (3.5)
5.52 ghijk (+5)
5.29 F
10.02 jklm
11.18 ijkl (+11.5)
7.33 lmnopq (27)
9.5F
5.06 hijk
12.0 c (+139)
23.62 a (+360)
13.5 A
14.51 fghij
49.15 b (+243)
55.44 a (+284.4)
39.7A
5.75 ghijk
6.43 fghij (+12)
6.50 efghi (+13)
6.23 E
4.73 opq
5.38 lmnopq (+14)
3.29 q (30)
4.4I
1.90 no
4.68 ijk (+145.5)
9.00 d (+372)
5.19 F
6.33 mnopq
19.68 de (+211)
20.42 d (+221)
15.4C
2.01 mno
4.73 ijk (+135)
8.11 def (+311)
4.95 F
3.31 q
8.79 klmno (+165)
8.47 klmnop (+158)
6.8H
1.63 o
2.34 mno (+43.5) 3.81 klmn (+133) 2.59 H
9.24 klmno
12.15 hijk (+31)
10.02 jklm (+8.5)
10.4E
4.33 kl
6.74 efgh (+56)
11.20 c (+159)
7.42 D
6.91 lmnopq
17.68 def (+156)
16.92 defg (+146)
13.8D
3.85 klmn
4.49 jk (+16.6)
6.47 fghi (+68)
4.94 F
15.86 efgh
17.69 def (+13)
12.81 ghijk (18.6)
15.4C
8.05 def
11.76 c (+46)
18.29 b (+127)
12.70 B
3.90 q
5.05 opq (+30)
4.24 pq (+9)
4.3I
7.39 defg
8.44 de (+14)
8.94 d (+21)
8.26 C
14.75 fghi
16.85 defg (+15)
10.66 ijklm (27.5)
14.0D
4.70 ijkk
8.21 def (+75)
11.63 c (+147.4) 8.18 C
3.79 q
10.80 ijklm (+185)
9.81 klmn (+160.6)
8.10G
4.23 C
6.29 B
9.54 A
9.22 C
17.45 A
16.11 B
Means in columns and row (interaction) for each treatment followed by the same lowercase letter(s) are not significantly different at the 5% probability level.
Values in the parentheses are the percentage reduction (-) or increase (+) compared with the control (I1).
Means in each column (main effect of genotype) or row (main effect of drought level) for each treatment followed by the same uppercase letter(s) are not significantly
different at the 5% probability level.
Table 4. Effects of drought level (D), genotype (G), and their interaction on the chlorophyll a and b contents of sesame under three levels of drought
(I1, I2 , and I3 = 55, 75, and 85% depletion of available soil water).
Chlorophyll a
Accession
Rashtkhar
Khaf2
Khaf1
Isfahan4
Shahreza
Borazjan
Ahvaz
kal
Shiraz
Markazi
Ardestan
Ultan
Isfahan1
Avg.
I1
I2
Chlorophyll b
I3
Avg.
I1
I2
I3
Avg.
mg g1
2.11 klmno
3.87 a (+83)
3.72 ab (+77)
3.23 A
0.593 no
1.20 abc (+103)
1.00 cdefghi (+69)
0.934 C
3.07 bcdefg 2.36 ijklmn (23) 2.10 klmnop (31)
2.51 E
0.933 efghijk
0.853 fghijkl (9)
0.820 ghijklmn (13) 0.868 D
3.37 abcde
2.56 fghijkl (24)
2.31 ijklmn (32) 2.75 CD
1.11 abcde
0.923 efghijk (17)
0.890 efghijkl (20) 0.976 BC
1.89 nop
3.57 abc (+86)
3.18 bcdef (+68)
2.88 BC
0.796 ghijklmn
1.24 ab (+56.5)
1.24 ab (+57)
1.09 A
3.37 abcde 2.83 defghij (16) 2.54 fghijklmn (25) 2.91 B
1.19 abcd
1.04 bcdefg (12.5) 0.976 defghij (18.5)
1.07 A
1.45 p
2.18 jklmno (+50) 1.93 mmop (+33)
1.85 G
0.566 o
0.783 ijklmno (+38) 0.820 ghijklmn (+43.5) 0.723 F
2.04 lmnop 2.61 efghijkl (+28) 2.22 jklmno (+9)
2.29 F
0.610 mno
0.773 jklmno (+27) 0.783 hijklmno (+29) 0.722 F
2.83 cdefghi 3.38 abcde (+19)
2.79 efghij (2)
3.00 B
0.913 efghijk 1.08 abcdef (+18.5)
0.903 efghijk (1.2) 0.965 BC
1.89 nop
2.72 defghijk (+44) 2.10 klmnop (+12)
2.24 F
0.796 hijklmn
0.730 klmno (8)
0.720 klmno (10)
0.748 F
3.07 bcdefg 2.67 fghijkl (13)
2.39 hijkl (22)
2.71 D
0.893 efghijkl
0.830 ghijklm (7)
0.823 ghijklm (9)
0.848 DE
2.93 cdefghi
3.04 cdefg (+4)
2.95 cdefghi (1)
2.98 B
0.963 efghij
1.02 cdefgh (+6)
0.963 efghij (1.5)
0.983 B
3.47 abcd
2.96 cdefghi (15) 2.45 ghijklmn (29) 2.96 B
1.29 a
1.07 abcdef (17)
0.976 defghij (24.4)
1.11 A
1.59 op
2.33 ijklmn (+46) 2.03 lmnop (+28)
1.98 G
0.666 lmno
0.906 efghijk (+36)
0.893 efghijkl (+33)
0.822 E
2.54 B
2.85 A
2.51 B
0.87 C
0.95 A
0.90 B
Means in columns and row (interaction) for each treatment followed by the same lowercase letter(s) are not significantly different at the 5% probability level.
Values in the parentheses are the percentage reduction (-) or increase (+) compared with the control (I1).
Means in each column (main effect of genotype) or row (main effect of drought level) for each treatment followed by the same uppercase letter(s) are not significantly
different at the 5% probability level.
Table 5. Effects of drought level (D), genotype (G), and their interaction on total chlorophyll and carotenoid content of sesame under three levels of
drought (I1, I2 , and I3 = 55, 75, and 85% depletion of available soil water).
Total chlorophyll
Accession
Rashtkhar
Khaf2
Khaf1
Isfahan4
Shahreza
Borazjan
Ahvaz
kal
Shiraz
Markazi
Ardestan
Ulan
Isfahan1
Avg.
I1
I2
Carotenoid content
I3
Avg.
I1
I2
I3
Avg.
mg g1
2.70 lmno
5.07 a (+88)
4.73 ab (+75)
4.17 A
0.167 nopq
0.248 defghij (+46.4)
0.299 abcd (+79)
0.238 DE
4.00 bcdefgh 3.24 h-m (19.6)
2.94 jklmn (27)
3.39 E
0.263 abcdefg
0.190 lmnopq (28)
0.177 mnopq (33)
0.210 G
4.49 abcd 3.47 fghijk (22.4) 3.24 hijklm (28.6) 3.73 C
0.252 cdefghi 0.209 ghijklmn (17) 0.197 ijklmnop (22) 0.220 FG
2.69 lmno
4.76 ab (+77.4)
4.42 abcde (+65) 3.95 B
0.183 lmnopq 0.248 defghij (+35.6)
0.312 ab (+72)
0.247 CD
4.57 abc
3.87 cdefgh (15) 3.49 fghijk (23.5) 3.98 B
0.202 hijklmno
0.171 nopq (15)
0.140 q (31)
0.171 I
2.02 o
2.97 jklmn (+47) 2.72 klmno (+36) 2.57 H
0.145 pq
0.202 hijklmno (+39) 0.239 efghijk (+64.4) 0.195 H
2.65 mno 3.39 fghijklm (+28) 3.01 ijklmn (+13.4) 3.02 F
0.176 mnopq
0.233 fghijkl (+32)
0.275 abcdef (+55.6) 0.228 EF
3.75 defghi
4.49 abcd (+19)
3.69 efghij (1.6) 3.79 B
0.232 fghijkl
0.245 defghij (+6)
0.289 abcde (+24)
0.255 C
2.69 lmno 3.45 fghijkl (+28.4) 2.85 klmn (+5.4)
2.99 F
0.244 efghijk
0.259 bcdefg (+6)
0.306 abc (+29)
0.269 B
3.96 cdefgh 3.49 fghijk (12)
3.29 hijklm (19) 3.51 E
0.255 defgh
0.227 ghijklm (13)
0.227 fghijklm (13)
0.236 E
3.90 cdefgh 4.07 bcdef (+4.3)
3.87 cdefgh (1) 3.57 D
0.257 bcdefg
0.270 abcdef (+5.5)
0.315 a (+23)
0.281 A
4.76 ab
4.03 bcdefg (15) 3.43 fghijkl (28) 4.07 AB
0.279 abcdef 0.236 efghijkl (15.5) 0.196 jklmnop (29.5) 0.237 DE
2.25 no
3.29 ghijklm (+43) 2.95 jklmn (+29.5) 2.83 G
0.149 opq
0.184 lmnopq (+23.6) 0.246 defghij (+64)
0.193 H
3.42 B
3.81 A
3.43 B
0.216 C
0.225 B
0.247 A
Means in columns and row (interaction) for each treatment followed by the same lowercase letter(s) are not significantly different at the 5% probability level.
Values in the parentheses are the percentage reduction (-) or increase (+) compared with the control (I1).
Means in each column (main effect of genotype) or row (main effect of drought level) for each treatment followed by the same uppercase letter(s) are not significantly
different at the 5% probability level.
115
Table 6. Effects of drought level (D), genotype (G), and their interaction
on seed yield of sesame under three levels of drought (I1, I2 , and I3 =
55, 75, and 85% depletion of available soil water).
Seed yield
Accession
I1
I2
I3
Avg.
g
Rashtkhar 3.17 fgh
2.94 ghi (7)
2.80 hijk (12)
2.97 E
Khaf2
5.86 a
2.60 hijkl (55.6) 2.41 ijklmn (59)
3.62 B
Khaf1
5.28 ab 2.60 hijklm (51) 2.13 klmno (59.6) 3.34 CD
Isfahan4
4.32 cd
3.99 de (7.6)
3.88 def (10)
4.06 A
Shahreza
3.57 efg 2.21 jklmn (38.4)
1.35 p (62)
2.37 H
Borazjan
3.60 efg
3.27 fgh (9)
2.90 ghij (19)
3.25 D
Ahvaz
3.77 def
3.21 fgh (15)
2.84 hijk (24.7)
3.27 D
Kal
3.71 def 2.72 hijkl (26.5) 2.30 ijklmn (38)
2.91 E
Shiraz
3.19 fgh
2.74 hijkl (14)
2.18 klmn (31.6) 2.70 F
Markazi
5.84 a
3.75 def (36)
2.71 hijkl (53.5)
4.01 A
Ardestan
4.38 cd
3.22 fgh (26.4)
2.82 hijk (35.6)
3.48 C
Ultan
2.34 bc
1.45 op (38)
1.19 p (49)
2.52 G
Isfahan1
2.07 lmno 1.89 mnop (9)
1.76 nop (15)
1.90 I
Avg.
4.13 A
2.81 B
2.40 C
Means in columns and row (interaction) for each treatment followed by the
same lowercase letter(s) are not significantly different at the 5% probability level.
Values in the parentheses are the percentage reduction (-) or increase (+)
compared with the control (I1).
Means in each column (main effect of genotype) or row (main effect of drought
level) for each treatment followed by the same uppercase letter(s) are not significantly different at the 5% probability level.
RWC
2
12
13
1
10
3
6
8
5
9
4
11
7
LSS
3
11
12
4
13
1
5
8
5
9
9
10
2
LP
3
11
13
2
12
1
4
7
5
9
8
10
6
RP
2
10
11
1
13
3
5
8
6
9
7
12
4
Total
chl
1
11
13
2
10
3
5
8
6
9
7
12
4
Carot
1
13
10
2
12
3
4
6
5
8
8
11
3
SY
2
11
12
1
13
4
5
8
6
10
7
9
3
Table 8. Correlation coefficients among measured traits in sesame under stress conditions (upper triangle, bold type) and normal conditions
(lower triangle).
Trait RWC
SY
Chl Carot LP
LSS
RP
SSI
RWC 1.00 0.36
0.21 0.36 0.05 0.57* 0.3 0.61*
SY
0.07 1.00
0.37 0.64* 0.5 0.18 0.68* 0.64*
Chl
0.07 0.65* 1.00 0.41 0.31 0.30 0.60* 0.24
Carot 0.05 0.72** 0.80** 1.00 0.52 0.02 0.47 0.72**
LP
0.18 0.27
0.55* 0.52 1.00 0.06 0.49 0.45
LSS
0.41 0.323 0.16
0.07 0.02 1.00 0.2
0.48
RP
0.07 0.71** 0.36
0.48 0.02 0.33 1.00 0.53*
SSI
0.46 0.58* 0.83** 0.63* 0.19 0.35 0.35
1.00
RWC, leaf relative water content; SY, seed yield; Chl, total chlorophyll; Carot,
carotenoid; LP, leaf proline; LSS, leaf soluble sugars; RP, root proline; SSI, Stress
Susceptibility Index.
* Significant at the 0.05 probability level.
** Significant at the 0.01 probability level.
116
smallest reduction in the seed yield (Table 6). In line with our
results, Mensah et al. (2006), Hassanzadeh et al. (2009a), and
Saeidi et al. (2012) also reported that the seed yield of sesame
genotypes was reduced under drought conditions, but with
differences among the genotypes.
Severe reduction in the seed yield of some accessions could be
due to reductions in RWC, leaf soluble carbohydrate contents,
leaf and root proline, chlorophyll, and carotenoid, as indicated
in Tables 2, 3, and 5, respectively. In other words, plants
invested more in stress protection mechanisms than in growth
when subjected to drought stress. Moreover, a reduction in
seed yield may also be due to less availability of nutrients along
with lower translocation of photosynthates from source to sink
areas under drought stress, as suggested by Manivannan et al.
(2007), Sankar et al. (2007), and Farooq et al. (2009).
Based on the overall results under I3 drought stress, we
were able to classify the accessions into tolerant (Isfahan4,
Rashtkhar, and Borazjan), moderately tolerant (Isfahan1,
Ahvaz, Shiraz, Ardestan, and Kal), and sensitive (Markezi,
Ultan, Khaf2, Khaf1, and Shahreza) groups. The accessions
were also ranked from 1 to 13 (1 = highest and 13 = lowest)
according to the measured traits under the I3 drought level
(Table 7). Isfahan4 had the first rank based on its RWC,
root proline content, and seed yield and second rank among
the other traits, except leaf soluble carbohydrate contents,
while Rashtkhar was ranked first based on its carotenoid and
chlorophyll contents and ranked second based on other traits
except soluble carbohydrates and leaf proline content.
A significant and positive correlation was also found
between seed yield and carotenoid (r = 0.64) and root proline
(r = 0.68) under drought (Table 8). These results again showed
the importance of carotenoid and root proline as markers for
resistance to drought stress. There were also significant and
positive correlations between soluble sugars and RWC (r =
0.57, p < 0.05) and root proline content and total chlorophyll
(r = 0.60, p < 0.05).
The SSI was calculated to assess the drought tolerance of
the sesame genotypes; the values of SSI for the accessions
were 0.19 (Isfahan4), 0.23 (Rashtkhar), 0.27 (Isfahan1), 0.35
(Borazjan), 0.45 (Ahvaz), 0.57 (Shiraz), (0.64) Ardestan,
0.68 (Kal), 0.89 (Ultan), 0.97 (Markezi), 1.06 (Khaf2), 1.08
(Khaf1), and 1.12 (Shahreza). Accessions estimated as drought
tolerant on the basis of lower yield reduction under drought
and other physiological characteristics generally had lower
SSI values. A lower SSI indicates a higher drought tolerance
(Fischer and Maurer, 1978). To explore relationships among
potentially useful traits to be used in breeding programs for
drought tolerance, the correlation coefficients between SSI
and the estimated parameters are given in Table 8. There was a
negative correlation between SSI and RWC (0.61, p < 0.05),
carotenoid (0.72, p < 0.01), and root proline (0.53, p < 0.05).
The negative correlation (more parameters, accessions with
lower sensitivity) between SSI and the mentioned parameters
suggest that these parameters may be used as selection criteria
for sesame genotypes in drought-stressed environments. These
results are in agreement with Gunes et al. (2008), Sairam and
Saxena (2000), and Talebi et al. (2013). In general agreement
with our results, Talebi et al. (2013) reported a significant
correlation between SSI and RWC in 35 chickpea genotypes
Agronomy Journal Volume 106, Issue 1 2014
117
118