Documente Academic
Documente Profesional
Documente Cultură
1
2
3
4
5
6
7
Volume of
0.1% starch
solution
0.00
0.50
1.00
1.50
2.00
2.50
3.00
Volume dH2O
(ml)
Volume I2
solution (L)
3.00
2.50
2.00
1.50
1.00
0.50
0.00
20.0
20.0
20.0
20.0
20.0
20.0
20.0
Starch
Concentration
(%w/v)
each test tubes were taken and were given 2.0 ml of distilled H 2O and a drop of I2
solution before measuring for absorbance at 620. The absorbance data was
converted to starch concentration was graphed vs. temperature.
D. Effect of pH on Enzyme Activity
In each four separated 10ml test tubes, the following were mixed: 1ml of
0.1% starch solution and 0.50 ml of 0.9% NaCl solution. Different pH (4.0, 6.7,
7.4, 9.0) of 3.5 ml 0.1 M phosphate buffers were added respectively on each of
the four separated test tubes. All tubes were added with 5 drops of enzyme
solution and were incubated at room temperature for 10 minutes before
immersing the tubes at boiling water for 3 minutes. The test tubes were cooled
and 1 ml aliquot of each test tubes were mixed with 2 ml of distilled H 2O and a
drop of I2 solution. The absorbance of each mixture were measured at 620 nm
and converted into starch concentration. The data gathered was graph into
starch concentration vs. pH.
Introduction
Enzymes are the catalyses of the reactions undergoing in our body. They
speed up reactions by finding an alternative reaction pathway, lowering the
activation energy and stabilizing the intermediates in the reaction. (Science,
2004).
References
Enzyme Kinetics as an Approach to Understanding Mechanism. (2011). Retrieved
from Bioinfo.org:
http://www.bioinfo.org.cn/book/biochemistry/chapt08/sim2.htm
J.H, W. (1971). Enzyme kinetics and its relevance to enzyme assay. Journal of
Clinical Pathology, 14-21. Retrieved from
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1176280/?page=2
Manila, U. o. (2013). Laboratory Manual in Biochemistry. Manila.
Massachusetts, U. o. (2015). Intro Biology. Retrieved from UMassAmherst:
http://bcrc.bio.umass.edu/intro/content/enzyme-kinetics-lab-protocol
Michaelis-Menten Kinetics and Briggs-Haldane Kinetics. (1925). Retrieved from
Michaelis-Menten Kinetics and Briggs-Haldane Kinetics:
http://depts.washington.edu/wmatkins/kinetics/michaelis-menten.html
Science, R. A. (2004). Enzymes. Retrieved from RSC:
http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm