ICSI in Hyderabad

ICSI (Intra cytoplasmic sperm injection) is an ART (assisted reproductive technology)

used to treat sperm related infertility problems. ICSI is used to improve the fertilization
phase of IVF (in vitro fertilization) by injecting the single sperm into mature egg. The
fertilized egg is then placed in a womans uterus/fallopian tube.

Importance:
1. Intra cytoplasmic sperm injection (ICSI) is used to take care of severe male infertility, as when
little or absence of sperm are ejaculated in the semen. Immature sperm composed from the
testicles are usually not able to move about and are more likely to fertilize an egg during ICSI.
2. Some couples might choose to try ICSI after frequent in vitro fertilization has been ineffective.
3. ICSI is also used for many couples who are planning to have genetic testing of the embryo to
check for certain inherent disorders. ICSI uses only single sperm for each egg. So there will be
no chance the genetic test can be infected by other sperm.

ICSI Procedure:

1. Sperm collection:Before collecting the sperm, male partner should be screened for possible
genetic problems that could pass on to the offspring . If the sperm cannot be collected through
masturbation, they must be surgically removed from the testicle through a small slit. This method
of sperm retrieval is done when there is blockage that avoid sperm from being ejaculated or
when there is a problem with the development of sperm.
2. Ovulation and egg retrieval:The woman should be injected with gonadotrophin or follicle
stimulating hormone (FSH) to inspire the ovaries to generate multiple eggs. After 6 to 7 days, the
doctor should check the blood estrogen altitude and use ultrasound to see whether eggs are
growing in the follicles. In the second week, the dosage may change based on test outcome and

ultrasound. If follicles fully develop, the woman is given a HCG (human chorionic
gonadotrophin) for the follicles to mature. The matured eggs should be collected and later using
needle aspiration directed by ultrasound through the pelvis to the ovaries.
3. Sperm injection and transfer:under high power magnification, an egg is held in place with a
glass pipette. Another pipette containing sperm is used to penetrate and inject one sperm to the
egg. After culturing in the lab overnight, eggs are checked for signs of fertilization. Two
Blastocysts or four day 2/3 embryos are placed in the uterus using embryo transfer catheter that
is inserted through the cervix. The remaining embryos may be frozen (cryopreserved) for future
efforts.
Website: http://www.kiranivfgenetic.com