2007 Installing Your Chemstation

Agilent ChemStation for
LC Systems
Installing Your ChemStation

Installing ChemStation for LC Systems
Agilent Technologies
Notices
Agilent Technologies, Inc. 2004,
2005-2007
No part of this manual may be reproduced in any form or by any means
(including electronic storage and
retrieval or translation into a foreign
language) without prior agreement
and written consent from Agilent
Technologies, Inc. as governed by
United States and international copyright laws.
Manual Part Number

G2170-90024
Edition
02/2007
Printed in Germany
Hewlett-Packard-Strasse 8
76337 Waldbronn
Software Revision
This guide is valid for B.03.xx revisions of the Agilent ChemStation for
LC Systems software, where xx refers
to minor revisions of the software
that do not affect the technical accuracy of this guide.
Microsoft is a U.S. registered trademark
of Microsoft Corporation.
Warranty
The material contained in this document is provided as is, and is subject to being changed, without notice,
in future editions. Further, to the maximum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a particular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or performance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the separate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accordance with the terms of such license.
Restricted Rights Legend

If software is for use in the performance of a U.S. Government prime
contract or subcontract, Software is
delivered and licensed as Commercial computer software as defined in
DFAR 252.227-7014 (June 1995), or
as a commercial item as defined in
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clause. Use, duplication or disclosure
of Software is subject to Agilent Technologies standard commercial
license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than
Restricted Rights as defined in FAR
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Government users will receive no
greater than Limited Rights as
defined in FAR 52.227-14 (June 1987)
or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated conditions are fully understood and
met.
WA R N I N G
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indicated conditions are fully understood and met.
In This Guide
In This Guide
This guide describes how to install the initial software, how to add further
instrument modules, how to configure your analytical system, and how to
verify that the installation and configuration are complete and operational.
Installing Agilent ChemStation

This chapter provides a description of the installation process. It gives you an
overview of the complete installation procedure, starting with the PC and operating system requirements and ending with the connection of the analytical
instruments. The installation of the Agilent ChemStation software itself is described in detail in this chapter.
Prerequisites
This chapter lists the minimum requirements for successful operation of the
Agilent ChemStation software. It provides detailed information about the requirements that must be met to ensure proper operation of the Agilent ChemStation. Requirements include PC type and performance, network protocol,
printer type, GPIB/LAN cards, USB-GPIB Interface, and the operating system.
Installing Communication Components

This chapter gives the background information you need in order to prepare
your Agilent ChemStation hardware and operating environment. Verify that
your PC meets the hardware and software requirements for the Agilent ChemStation and do the necessary adjustments where appropriate. If you bought
your Agilent ChemStation as a bundle from the Agilent Technologies Chemical
Analysis Group, these steps have already been performed for you. In this case,
PC hardware has already been set up, the LAN card has been installed and configured, the operating system and operating environment have been preloaded
on your computers hard-disk, and the Agilent ChemStation has been installed.
Installing the Analytical Instruments

This chapter describes how to connect the HP 1090, HP 1046, HP1049, and Agilent 1100/1200 LC analytical instruments (and specific detectors) to the Agilent ChemStation using LAN, GPIB, or USB-GPIB connection. Default GPIB
address settings and firmware requirements for the LC Instruments are also
described.
In This Guide
Checking Out the Agilent ChemStation

This chapter describes how to perform a short system integrity test. The steps
outlined in the chapter help you when validating your system intallation.
Configuring the Analytical System

The first section in this chapter describes how to configure the communication
of your Agilent ChemStation software with your LC instruments using a GPIB
or LAN connection with the Agilent ChemStation Configuration Editor. The
second section describes how to configure a BootP Service that can be used for
communication using a LAN connection.
Troubleshooting
This chapter will provide elementary troubleshooting information in case
something goes wrong. The chapter will cover the most common software
start-up problems, printer problems and computer problems and how to overcome them.
Upgrade History - Previous Revision A/B ChemStations

With Agilent ChemStation B.01.0x release the Agilent ChemStation made a progressive move towards new feature sets and structure changes. Due to this the
new Agilent ChemStation is starting with a new major revision change: B.01.0x.
Within this Chapter a summary of the introduced new functionality for the revision cycle A03.x till B.02.x is documented.
Supplementary Information
Contents
Contents
1
Installation Procedure
Contents of the two Agilent ChemStation CD-ROMs
Agilent ChemStation Product Structure
Installing the Agilent ChemStation
Uninstalling the Agilent ChemStation
8
9
13
15
22
Prerequisites
25
Agilent ChemStation PC Hardware Details

Operating System Requirements
Number of Instruments Supported
26
30
31
33
Installing LAN Communication

GPIB and USB-GPIB Communication
34
38
61
Connecting an Agilent 1100/1200 Series LC to the Agilent ChemStation

Connecting an HP 1090 LC to the Agilent ChemStation
Connecting Miscellaneous Instruments to the Agilent ChemStation
LC 1100/1200 Series Instrument Firmware Requirements
1090 and Miscellaneous LC Instrument Firmware Requirements
62
66
70
72
76
77
Agilent ChemStation Installation Verification

Qualifying Operation Agilent ChemStation Verification Test
78
83
85
Using the Agilent ChemStation Configuration Editor

Example for LC Configurations
Using the Agilent Bootp Service
86
90
94
Contents
Troubleshooting
103
Troubleshooting LAN Communication

Troubleshooting GPIB Interface Problems
Software Startup Problems
Printing Problems
Computer Problems
LC Instrument Start-up Problems
Using the WinDebug Utility
Gathering System Information
105
107
109
115
119
121
123
126
127
Introduction
ChemStation Revision Cycle A.x.x
ChemStation Revision Cycle B.x.x
128
129
134
135
Agilent ChemStation Revision Code Convention

Agilent Technologies Support Services
Calling the Agilent Technologies LSCA Customer Contact Center
136
137
138

1 Installing Agilent ChemStation

Agilent ChemStation Modules
Installation Verification Utility for Installation Qualification
I/O Libraries for the Agilent 82350 A/B GPIB Interface and the
82357A USB-GPIB Interface
10
BootP Service
10
Agilent ChemStation Software Status Bulletin (SSB)
10
Agilent ChemStation Revision History
10
Agilent ChemStation Product Documentation
11
What Is Reference Information and Where Is It?
11
What Is Task-Oriented Information and Where Is It?
12
Where Can I Find the Description of Agilent ChemStation

Commands and Data Structures?
12
I Upgraded from a Previous Version - How Do I Know What Has

Changed Since Then?
12
13
Additional Data Evaluation Modules
13
15
Overview
15
15
Activating the XML based interface
21
22
This chapter provides a description of the installation process. It gives you an

overview of the complete installation procedure, starting with the PC and operating system requirements and ending with the connection of the analytical
instruments. The installation of the Agilent ChemStation software itself is described in detail in this chapter.

To ensure the successful installation of the Agilent ChemStation and the

connection of analytical instruments, we strongly recommend to follow the
following step by step procedure:
1 Check the computer and operating systems requirements in
Prerequisites on page 25.
2 Install the Agilent ChemStation software as described in Checking Out the
Agilent ChemStation on page 77.
3 If required install and configure the drivers for GPIB or USB-GPIB
communication or if necessary the BootP Service as described in Installing
Communication Components on page 33.
4 Install the analytical instruments as described in Installing the Analytical
Instruments on page 61.
5 Configure the Agilent ChemStation software according to the chosen
installation as described in Configuring the Analytical System on page 85
6 Examine the installation as described in Checking Out the
NOTE
To perform an upgrade from a previous ChemStation to B.03.xx please refer to the Upgrade
Preparation Guide for Agilent ChemStation for detailed instructions. The documentation is
available as printed document as well as PDF file in the manual directory of the
ChemStation CD-ROM (CD 2 - User Documentation and Accessories).

Starting with the ChemStation Release B.03.01, the Agilent ChemStation comes
along with two CD-ROMs. The CD-ROM contents are splitted up in the following
parts
CD 1 - Installation containing all Installables, Firmware and
Communication tools in order to install your system
CD 2 - User Documentation and Accessories containing ChemStation
Manuals, Support Documentation and Software Accessories e.g. User
Contributed Library (UCL)
Agilent ChemStation Modules

The CD-ROM (CD 1 - Installation) contains the executable files for all modules
that belong to the Agilent ChemStation product family. In order to install a
module, you have to supply a license registration number valid for the module.
Those numbers unlock the protected CD-ROM (CD 1 - Installation). They are
included with the original product and are your proof of license.
There is a main setup program setup.exe for the Agilent ChemStation modules
located in the root directory on the Agilent ChemStation CD-ROM (CD 1 Installation).
Installation Verification Utility for Installation Qualification

The Agilent ChemStation comes with a utility that allows to qualify and
document the completeness and integrity of a new installation. The
Installation Verification Utility is installed automatically with the Agilent
ChemStation.

I/O Libraries for the Agilent 82350 A/B GPIB Interface and the
82357A USB-GPIB Interface
The Agilent ChemStation CD-ROM (CD 1 - Installation) contains the version of
the I/O Libraries that have been successfully tested with B.03.xx of the Agilent
ChemStation. The I/O Libraries must be installed separately to be used with
the GPIB cards as well for the USB-GPIB device. The installation is described
in Configuring the Agilent 82350 GPIB Interface Drivers on page 41.
BootP Service
The Agilent ChemStation CD-ROM(CD 1 - Installation) contains a BootP
Service setup in the directory BOOTP that can be used to supply the analytical
instruments that are connected to a LAN with an IP address and configuration
settings. This BootP Service is easy to configure for the use of analytical
instruments using a LAN connection. Refer to section What is the Agilent
Bootp Service? on page 94 for more information about the BootP Service.
Agilent ChemStation Software Status Bulletin (SSB)

The Software Status Bulletin is a document reflecting the results of Agilent
Technologies defect logging, tracking, and repair methodology by publishing
the current status of Agilent ChemStation application software products
(known defects, available fixes, additional information).
The SSB is located in the SUPPORT\SSB directory of the Agilent ChemStation
CD-ROM (CD 2- User Documentation and Accessories).
Agilent ChemStation Revision History

The revision history is targeted towards users who may have to consider
revalidating their analytical data system after upgrading to a new revision of
the application software. The history files are located in the SUPPORT\
HISTORY directory of the Agilent ChemStation CD-ROM (CD 2- User
Documentation and Accessories).
10

Agilent ChemStation Product Documentation

The Agilent ChemStation product documentation consists of paper handbooks
with reference information and online documentation for task-orientated
topics. The manuals are also located on the second ChemStation CD-ROM (CD
2 - User Documentation and Accessories) in the directory MANUALS, together
with the required Adobe Acrobat reader.
For first time users a Tutorial is available in the online help of the
Agilent ChemStation and documents in detail the first necessary steps to
control and run an Agilent 1100/1200 LC System.
The implemented XML interface is fully documented in the XML Connectivity
Guide, which is also available as a PDF document on the Agilent ChemStation
CD-ROM (CD 2 - User Documentation and Accessories). For the ECM
Integration with ChemStation, please refer to the ECM Interface Guide as well
available in the manual section.
Documentation regarding the LC used standard LAN communication card
(G1369A LAN card) is available as a PDF document on the
Agilent ChemStation CD-ROM (Agilent G1369A LAN Interface Manual) (CD 2
- User Documentation and Accessories).
What Is Reference Information and Where Is It?

With the documentation strategy for the Agilent ChemStation chosen by
Agilent Technologies, all reference information, i.e. explanation of general
concepts, algorithms, and formulae is contained in the Understanding
handbooks. The reference handbooks generally do not provide any
task-orientated information (e.g. How to set up a sequence).
The Agilent ChemStation handbooks do not cover details on analytical
hardware (other than those required for installation and configuration for
operation with the Agilent ChemStation).
11

What Is Task-Oriented Information and Where Is It?

Task-oriented information describes how to perform a certain task and is
therefore very context-specific. The context-specific How-To information is
contained in the Agilent ChemStation help system. The help system always
can be evoked by pressing the F1 function key, the Agilent ChemStation Help
menu, or the Help button available in Agilent ChemStation dialog boxes.
Access the Help/Help Topics menu and click the LC Tasks or GC Tasks
index. Select the analytical task that you need instructions for.
Where Can I Find the Description of Agilent ChemStation

Commands and Data Structures?
The Macro Programming Guide and Commands Reference are available for
advanced users and Agilent ChemStation application developers. The
Commands reference information can be accessed from the
Agilent ChemStation help menu (Help/Commands) and is structured into
command categories and the command descriptions with syntax, parameters,
and return values along with a discussion and frequent examples.
The Macro Programming Guide is available electronically through the Agilent
ChemStation help system.
I Upgraded from a Previous Version - How Do I Know What Has

Changed Since Then?
When starting the Agilent ChemStation for the first time, you can select to go
to the Whats new section of the help. Later on you can always go to the table
of content of the help file and select new features to find out what features
have been added to the Agilent ChemStation.
For upgrades from Agilent ChemStation Rev. A.xx.xx to Rev. B.03.0x a
separate guide called Upgrade Preparation Guide for Agilent ChemStation
Rev. B.03.01 is available as printed manual as well as PDF document within
the manual section of the Agilent ChemStation CD-ROM (CD 2 - User
A list of all defect fixes since the last revision is located in the directory
support\history.
12

The Agilent ChemStation for GC, LC, CE, CE/MS, LC/MS, and A/D has been
designed as a series of software modules for instrument control, data
acquisition, data analysis, and reporting. Technique-specific single instrument
modules provide control capabilities for a given separation technique, e.g. for
one Gas or Liquid Chromatograph. A single instrument configuration may be
expanded by adding an additional instrument control module. With this
approach, one Agilent ChemStation is capable of controlling more than one
analytical system, e.g. two chromatographs of the same or different separation
techniques.
Please note the memory requirements for every additional instrument module
(see Minimum PC Hardware Requirements for the Agilent ChemStation on
page 27).
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Agilent ChemStation Module Architecture
For further information regarding Solution Add-Ons (e.g. Purify Software,

ChemStore, ECM) please contact your local administration.
Additional Data Evaluation Modules

The acquired data is typically two-dimensional (2D), i.e. by measuring
detector response over time. Spectroscopic detectors can deliver
three-dimensional (3D) data, by additionally measuring detector response
over a third axis (e.g. wavelength or mass range). The optional Spectral
13

Evaluation Module permits analysis and reporting of this 3D data and is
included with the CE, CE/MS, and LC/MS modules, and optional for an LC
module.
The Agilent ChemStore Database Module, a results storage and organization
module, may be added to all separation techniques.
The Agilent ChemStation is capable of controlling up to four instruments. The
number of allowed modules per instrument is depending on the used
communication module. For configuration limitations please refer to Number
of Instruments Supported on page 31. For CE/MS and LC/MS no additional
instruments are supported on the PC system. For CE additional CE, LC, or GC
instruments are supported only as co-existing instruments on the same PC,
but co-execution is not supported.
Please note the memory requirements for each additional data evaluation
module. For further details on supported configurations and their PC
requirements, see Minimum PC Hardware Requirements for the
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A Combination Of Modules

Overview
This section explains how to use the enclosed CD-ROM (CD 1 - Installation) to:
install the Agilent ChemStation for the first time
upgrade existing Agilent ChemStation software
install an additional Agilent ChemStation module
Use the following procedure if you are using the CD-ROM (CD 1 - Installation)
to install or upgrade the Agilent ChemStation.
Before installing the software:
NOTE
To perform an upgrade from an Agilent ChemStation Rev. A.xx.xx system to B.03.0x please
refer to the Upgrade Preparation Guide for Agilent ChemStation Rev. B.03.0x for detailed
instructions. The documentation is available as printed document as well as PDF file in the
manual directory of the Agilent ChemStation CD-ROM (CD 2 - User Documentation and
Accessories).
1 Set up the computer hardware: If you are going to connect your

instruments using a GPIB or USB-GPIB connection, install the
communication device as described in GPIB and USB-GPIB
Communication on page 38.
2 Install the Windows XP Professional operating system and the required
patches and/or service packs on the PC. Refer to the documentation
delivered with your PC or with the operating system. Start the operating
system. Please make sure that your logon has administrative capabilities, as
changes will be made to the ChemStation.ini file (location: c:\windows )
and the Windows registry.
15

Set up the computer settings:
It is recommended that the Regional options in the Control Panel of your
System are set to English (US). If another location is used, the following
settings are mandatory:
Decimal symbol = . (point)
Digit grouping symbol = , (comma)
List seperator = , (comma)
When a non-bundled PC is used, please follow the instructions outlined
in the Configure and Maintain your Agilent ChemStation Computer
document, which is available as a PDF document on the
Agilent ChemStation CD-ROM (CD 2 - User Documentation and
Accessories). This documentation describes required system settings for
best Agilent ChemStation performance.
Keep your PC disconnected from the Internet during the installation
phase. It is strongly recommended to install the latest security fixes as
supported from Agilent and virus definitions before connecting to any
network.
3 Check the partitioning scheme of your PC and ensure that the Agilent
ChemStation is installed on a sufficiently large partition and that the path
to the data directories is configured to a sufficiently large partition (see
Configuring Paths on page 88).
4 Insert the Agilent ChemStation CD-ROM (CD 1 - Installation) into the
CD-ROM drive.
5 From the Start menu in the Task Bar, select Start > Settings > Control Panel.
6 At the command line, type diskdrive:\Setup (e.g.,D:\Setup), then click
on OK. The Install Wizard will start immediately.
If this is an installation on a new computer, proceed with step 8, if you would
like to upgrade the system or to add an additional instrument proceed with
steps 6 and 7 as well.
16

Figure 3
If you are upgrading your system or adding an additional module, click on
Upgrade to continue. With the next step you have to choose between upgrade or
installing additional instruments:
Figure 4
Choosing No the user can add additional instruments by using the initial
installation screen.
Choosing Yes enables the upgrade routine and the Setup program updates all
existing Agilent ChemStation software on your PC. This guarantees all Agilent
ChemStation instrument modules have the same version.
17

After updating the existing Agilent ChemStation installation, you can specify
whether to install additional modules such as additional instrument control or
add-on software modules. If no, you may skip the following steps and start
working with the new version of the Agilent ChemStation.
Figure 5
7 If you need to install an additional module, specify the number of the
instrument that you want to add or modify.
Figure 6
Select an instrument number and click on Add/Change.
18

Figure 7
Configuration Window
8 Agilent ChemStation B.03.01 uses Microsoft .NET Framework 2.0, which

improves both security and speed of applications and generates a basis for
programs of various programming languages. If Microsoft .NET 2.0
Framework is not already installed on your computer, the
Agilent ChemStation installation notices you to run its installation.
Microsoft.Net Framework is available on the ChemStation CD-ROM (CD 1 Installation), directory dotnetFramework 2.0 (CD 1 - Installation). The
19

upcoming message displays the location of the file. Execute the file
dotnetfx20.exe.
9 Select the appropriate software module from the list of available products
in the Configure Instrument dialog and press Add (see Figure 7 on page 19).
10 Enter the License Registration Number for the selected module in the New
License Number Field and press Add.
The applicable product and license numbers are printed on your Software
Certificate and Registration Packet (see the example license registration
label).
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4
Figure 8 Example License Registration Label

If you need to install another module for this instrument, e.g. an additional
data evaluation module, proceed with step 6. If you have purchased more
than one instrument control module license for this PC, click OK and
proceed with step 7 to install it as an additional instrument.
11 Check that the path points to the correct partition, default is C:\. Click on
Install of the Instrument Setup box to continue.
NOTE
The Agilent ChemStation will add a path statement into the operating system environment
variable PATH, e.g. c:\Chem32;c:\Chem32\sys
12 The Setup program will prompt you to configure additional new

instruments you installed, for fresh installation the configuration editor
appears directly on screen. If you want you can skip this option and
configure the instruments later.
20

Figure 9
Click on Yes to access the Agilent Configuration Editor and configure your
instruments. Click on No to exit the Setup program and return to Windows.
13 Your software installation is now complete. The Setup program made
changes to your CHEMSTATION.INI file and saved a copy of the original
CHEMSTATION.INI file in the files named ChemStation.XXX (i.e.,
ChemStation.000, ChemStation.001, ChemStation.002, ChemStation.003) in
the directory C:\CHEM32\BACKUP.
Remove and store your CD-ROM (CD 1 - Installation) in a safe place.
Save your license numbers in a safe place. These will be required, if you
have to reinstall your software or for future upgrades.
14 After you install and configure the Agilent ChemStation software, be sure
to exit Windows and reboot your system before starting the
Agilent ChemStation.
Activating the XML based interface

In order to activate XML support, you need to make changes to the
CHEMSTATION.INI file. For full details see the XML Connectivity Guide on
the Agilent ChemStation CD-ROM (CD 2 - User Documentation and
Accessories).
21

In some cases you might need to uninstall the Agilent ChemStation, for
example, if you wish to install it in a different location.
To completely remove a Agilent ChemStation installation you can use the
standard Windows uninstall procedure (Control Panel > Add/Remove programs).
For uninstallation, please proceed with the following steps:
NOTE
If any other products belonging to the Agilent ChemStation Plus Family are installed the
uninstall routine alerts that an Add-On Solution (such as ChemStore or ECM) has been
detected. Add-On solution software needs to be uninstalled using the standard Windows
uninstall procedure (Control Panel > Add/Remove programs). Please uninstall these
products using the Windows routine PRIOR to uninstalling Agilent Chemstation B.0x.0x.
This also includes all Service Releases or Patches for the Agilent ChemStation that may
have been delivered for the Agilent ChemStation revision you want to uninstall. The system
may need you to manually modify the ChemStation.ini file. Please refer to the
corresponding software documentation of the Agilent ChemStation Plus products for
details.
1 If the Agilent ChemStation is running close all sessions and reboot the
computer.
2 Select Start > Settings > Control Panel > Add/Remove Programs. Select Agilent
ChemStation B.03.01 and press Change/Remove. The wizard starts and the
system will ask you wether to perform an upgrade or to remove the Agilent
ChemStation. Continue by selecting the Remove options and press Next.
22

Figure 10
3 Before starting the uninstalling you will be prompted to verify the
deinstallation before proceeding. Click Yes to continue.
Figure 11
4 During uninstallation the system will backup your Agilent ChemStation
data, methods, sequences, UV-libraries, customized report styles, tune files
23

(for CE/MSD systems), and if present any customized macros such as
user.mac.
The system creates a directory called e.g. c:\Chem32_Backup to store the
data. The original structure of the data will remain, e.g.:
\CHEM32_Backup\1\
\CHEM32_Backup\2\
\CHEM32_Backup\3\
\CHEM32_Backup\4\
containing all data, methods, sequences, and tune files (in case CE-MSD
addon was installed) for instrument 1 to 4
\CHEM32_Backup\REPSTYLE contains your customized reports
\CHEM32_Backup\SPECLIBS contains your spectral libraries
\CHEM32_Backup\CHEMSTOR contains local Agilent ChemStore
databases
\CHEM32_Backup\CORE might contain custom macro user.mac
5 The complete CHEM32 folder is removed from your hard disk.
6 The Agilent ChemStation path statement is removed from the environment
path variable within the Windows operating system environment.
24

2 Prerequisites
26
Minimum PC Hardware Requirements for the

Agilent ChemStation
27
Non-HP Computers
27
Printers for the Agilent ChemStation
28
Advanced Power Management Not Supported with Analytical

Hardware
29
File Names and Naming Conventions
30
30
31
This chapter lists the minimum requirements for successful operation of the
Agilent ChemStation software. It provides detailed information about the requirements that must be met to ensure proper operation of the Agilent ChemStation. Requirements include PC type and performance, network protocol,
printer type, GPIB/LAN cards, USB-GPIB Interface, and the operating system.
25
Prerequisites
This section states the PC hardware and operating system requirements that
must be met for successful installation and operation of the
The Agilent ChemStation B.03.01 and greater is supported on personal
computers with an Intel Pentium IV 1.5 GHz (or higher).
Using a GPIB interface one PCI slot is required. For details about GPIB
interface requirements refer to the section GPIB and USB-GPIB
Communication on page 38.
If a LAN connection is used for the analytical equipment, a LAN interface
supported by the operating system is required and the Microsoft TCP/IP
protocol must be installed.
All PC hardware and peripherals must be listed in the Microsoft Hardware
Compatibility List (HCL) which is available from the Microsoft home page on
the world wide web (http://www.microsoft.com). If your PC hardware is not
listed in the HCL, the system may not work correctly with the Agilent
ChemStation software.
26
Prerequisites
Minimum PC Hardware Requirements for the Agilent ChemStation

Table 1
NOTE
Minimum PC Hardware Requirements for Windows XP Based Systems
Processor
Pentium IV 1.5 GHz
Screen resolution
Super VGA (1280 x 1024)
Mass storage
40 GB Hard-Disk and CD-ROM
Minimum Memory for Single/Multi Instrument 2D/3D
512 MB of RAM
Instrument configuration with Agilent ChemStore C/S

database module (Rev. B.03.03 SR2),
512 MB of RAM
Generally a maximum of four instrument systems may be configured to each Agilent

ChemStation, but there is a limitation of around 18 individual modules connected to the
Agilent ChemStation PC. Refer to Number of Instruments Supported on page 31.
Non-HP Computers
The Agilent ChemStation has been designed to successfully run on a wide
range of compatible personal computers equipped with accessories and
peripherals that adhere to the programming standards for the Intel PC
platform and Microsoft Windows operating systems.
However, Agilent Technologies has tested the Agilent ChemStation software
mainly on Agilent Technologies equipment. All configuration information
listed in this manual applies to Hewlett-Packard/Compaq Kayak, Vectra and
EVO computers and may not be optimized for other vendors PCs. The
standard configuration of the GPIB interface, for instance, may conflict with
the memory configuration of a non-HP computer.
Additional accessory interface boards may cause conflicts of hardware related
resources (I/O ports, interrupt settings, DMA channels).
For a non-Hewlett-Packard computer, use the setup utility program supplied
by the manufacturer to configure your computer and check the documentation
supplied with it and the accessories to eliminate resource conflicts in your
PCs setup, especially regarding the configuration of the GPIB interface.
27
Prerequisites
Printers for the Agilent ChemStation

The Agilent ChemStation has been designed to work with printers that are
compatible with the operating system. Printers may be attached to a local
(preferably parallel) or networked port on the PC. Serial port printers are
supported by the operating system but may exhibit speed performance
limitations. Networked printers must be shared by a network server running a
network protocol supported by the Microsoft operating system.
We recommend printers that are capable of interpreting an escape code
language (e.g. PCL) or page description language (e.g. PostScript). Host-based
printers (e.g. GDI or PPA printers) impose more printer processing tasks on
the CPU and are not recommended for use with Agilent ChemStation on-line
sessions.
For best printing results with your Agilent ChemStation, use HP LaserJet
printers. High performance HP DeskJet printers may also be used if the
amount of printing required is low. Check the readme.txt files for information
on recommended printer driver versions.
Agilent Technologies has not tested all printer and printer driver
combinations that are supported in the Windows environment. Print
performance and results may vary on other manufacturers printers and
appropriate drivers. The printers listed in Table 2 on page 28 have been
successfully tested at the time this handbook was printed.
NOTE
Some low-end printers may not be suitable for unattended high data-throughput operation
of the Agilent ChemStation due to limited print-performance (< 8 pages per minute) and
paper tray capacity (less than 150 pages), e. g. in multi-instrument configurations or when
running large sequences with many injections and relatively short run-times (e.g. less than
5 minutes), even if listed in Table 2 on page 28.
Table 2
28
Successfully tested Printers
Printer Model
Driver comment
HP LaserJet 4050 and HP LaserJet 4100
HP PCL 5e Driver or PCL 6
HP LaserJet 4200
HP PCL 5e Driver or PCL 6
HP LaserJet 4250
PCL 6 Driver
HP LaserJet P3005D
PCL 6 Driver
Prerequisites
NOTE
Printer Model
Driver comment
HP Color LaserJet 2500TN
PCL 6 Driver
OfficeJet Pro K550DTWN
PCL 3 Driver
Please note that this list is not comprehensive and does not include printers and printer
drivers made available after the release of this handbook. If your printer is not listed here, it
does not mean it will not work with the Agilent ChemStation, but it has simply not been
tested.
Advanced Power Management Not Supported with Analytical

Hardware
The BIOS and operating systems of many modern PCs supports Advanced
Power Management (APM). After a specified idle time, the BIOS will switch
the system to stand-by by turning off the computer display, hard disk, and
other devices, reducing the output of the PCs power supply and the internal
clock frequency to save energy.
The reduction in internal clock speed and hard disk slow down can render the
PC incapable of handling the real-time requirements of instrument control and
data acquisition. Typically, this results in overflows of the internal instrument
buffers, or, (in other words) data loss. APM is not supported in systems used
for on-line operation of analytical hardware.
29
Prerequisites
ChemStation Rev. B.03.01 is supported on Microsoft Windows XP Professional

(Service Pack 2).
It is available in three languages:
English
Japanese
Chinese
Depending on your choice of language you will need the corresponding
language version of the operating system.
NOTE
Agilent cannot give a support statement for other language versions of the operating
system.
Windows XP Professional is the only supported operating system for the

Agilent ChemStation data acquisition and analysis software. For up-to-date
information on supported operating systems please contact your local service
and support center.
NOTE
ChemStation Rev. B.03.01 is NOT supported on Windows 2000 Professional.
If you use a LAN to connect to the analytical instruments, the Microsoft

TCP/IP protocol must be installed and configured.
File Names and Naming Conventions

Detailed information regarding allowed characters used for file and directory
names, as well as path length statements are documented in the
Understanding your Agilent ChemStation manual, Chap. 1. The manual is
delivered with the instruments and available as PDF file on the
Agilent ChemStation CD-ROM (CD 2 - User Documentation and Accessories).
30
Prerequisites
A maximum number of 4 instruments can be configured on a single

Agilent ChemStation PC. Each instrument can consist of several modules, like
Agilent 1100/1200 LC modules, HP 1090 mainframe with DAD, HP 1046 FLD
etc. For specific configurations and software add-ons, the maximum number
of instruments is listed in Table 3 on page 31.
NOTE
While acquiring data in three or four instrument configurations, interactive data

reprocessing is not recommened.
Table 3
Maximum Number of Instruments
Description
max. Number of
instruments
ChemStore C/S Data Management Module
2 modules that can acquire on-line spectra, e.g. DAD or FLD
Agilent LC/MSD
There is also a limitation on the total number of instrument modules

connected to the Agilent ChemStation. Up to a maximum number of 18
modules per PC can be connected, depending on the exact module
configuration and LAN connection point. The maximum number of 18
modules can consit of approx. 12 modules like pumps, detectors, injectors, etc.
and approx. 6 can-slave modules like valves and UIB interfaces. A maximum of
4 detectors per instrument are supported, whereas only 2 of one kind can be
used in the same instrument, in case of the VWD up to 3. As soon as a G1315C
DAD Detector is used, the max. numbers of G1315C detectors is limited to two
detectors per PC.
NOTE
Please contact your Agilent representative to check if your exact configuration is possible,
especially when your configuration is above 12 modules.
31
32
Prerequisites

3 Installing Communication
Components
34
Installing TCP/IP on Windows XP Systems
34
Installing the Agilent BootP Service Program
35
Using fixed IP address for Communication
37
38
Instrument Compatibility Matrix
38
Agilent 82350 A/B GPIB Interface card
39
Installing a GPIB Interface Board in the PC
39
GPIB Cabling
39
Configuring the Agilent 82350 GPIB Interface Drivers
41
GPIB board Configuration with the Agilent ChemStation
46
Configuring and Installing the Agilent 82357A USB - GPIB

Interface and Driver under Windows XP Professional
46
This chapter gives the background information you need in order to prepare
your Agilent ChemStation hardware and operating environment. Verify that
your PC meets the hardware and software requirements for the Agilent ChemStation and do the necessary adjustments where appropriate. If you bought
your Agilent ChemStation as a bundle from the Agilent Technologies Chemical
Analysis Group, these steps have already been performed for you. In this case,
PC hardware has already been set up, the LAN card has been installed and configured, the operating system and operating environment have been preloaded
on your computers hard-disk, and the Agilent ChemStation has been installed.
33

If you decide to connect your instrument using a standard LAN connection,

you must ensure proper communication between the PC and the analytical
instruments. The communication uses the TCP/IP protocol, which needs to be
installed as a network protocol on your PC. For the configuration of the
JetDirect card or G1369A LAN card that is used to connect the analytical
instrument to the LAN, the boot strap protocol is used, which requires a BootP
Service.
This chapter outlines the installation of the TCP/IP protocol on Windows XP
systems. Windows XP systems will typically come with TCP/IP pre-installed.
Instructions are also included for the Agilent Bootp Service program required
to configure TCP/IP parameters for your instrument.
Installing TCP/IP on Windows XP Systems

1 From the Start menu in the Task Bar, select Start > Settings > Control Panel.
2 Open Network and Dial-up Connections, select Local Area Connection and
use right mouse click to access the properties.
3 In the General tab, select the internet protocol TCP/IP and choose
Properties.
4 Select Use the following IP address to ensure that the system does not use the
DHCP address (see section DHCP Server on page 106 for details).
5 Ask your IT department for a valid IP addresses, gateway, subnet mask,
DNS and WINS server, if your PC is connected to a site network. If you are
setting up your own internal network and are not connected to any other
network, you may use the example settings from Table 4 on
page 35Gateway, DNS, and WINS IP addresses do not need to be configured
in this case, as you do not connect to any other part of a network. Figure 12
on page 35 shows an example of the TCP/IP configuration on Windows XP.
NOTE
34
You need one IP address per device. This means one for the PC, another one for the MSD
and a third one for the 1100/1200 pump.

Table 4
Example IP addresses
PC or instruments
IP address
Subnet mask
PC 1
10.1.1.100
255.255.255.0
instrument module 1 (MSD)
10.1.1.101
255.255.255.0
instrument module 2 (1100/1200 pump) 10.1.1.102
255.255.255.0
...
255.255.255.0
Figure 12
....
TCP/IP Configuration Screen
6 Select OK to finish the configuration.
Installing the Agilent BootP Service Program

If you wish to use a local BootP Server/Service or if there is already a BootP
Server/Service running in your network, contact your system administrator
with the information supplied in Addresses on page 95.
35

The configuration of the Agilent BootP Service is outlined in Configure the
Agilent Bootp Service Program on page 96.
1 Logon as Administrator or other user with Administrator privileges.
2 Close all Windows programs.
3 Insert the Agilent ChemStation CD-ROM (CD 1 - Installation) into the
CD-ROM drive.
4 Open the Explorer and double-click x:\Bootp\Agilent Bootp Service.exe,
where x: is the CD-ROM drive letter.
5 Follow the instructions on the screen for the installation of the Agilent
BootP Service program. It is recommended that you read the readme.txt
file, that might contain last minute information not included in this manual.
6 When the installation is finished, the BootP Settings screen appears. The
BootP Settings screen contains unconfigured default settings. They will be
corrected during the configuration procedure.
Figure 13
36
BootP Settings

7 Select Maintain bootp tabfile?
8 Check Do you want to log bootp requests?
9 In the Default Settings part of the screen enter subnet mask and gateway. See
your network administrator if you do not know the subnet mask and
gateway. For standalone systems the default subnet mask 255.255.255.0
and no gateway can be used. If you would like to proceed already with the
configuration of your system, do not proceed with step 10. Continue with
the configuration of your instruments as outlined in Addresses on
page 95.
10 Click OK. The Install Wizard complete screen appears.
11 Select Yes, I want to restart my computer now. Remove the CD-ROM (CD 1 Installation)from the drive.
12 Click Finish. The computer will reboot.
The Agilent BootP Service program can now be edited using the Agilent Bootp
Service icon from the Agilent ChemStations group in the start menu to add
instrument.
Using fixed IP address for Communication

Since Revision A.09.1 it is possible to assign fixed IP address to the Agilent
1100/1200 modules, using the Control Module or Telnet to assign IP addresses.
The IP address will be stored in the non-volatile RAM of the modules. The
minimum required firmware for the G1369A LAN card is A.01.05.
For more details, please refer to the manual Agilent G1369A LAN Interface.
The manual is available as PDF document in the manual section of the
Agilent ChemStation CD-ROM (CD 2 - User Documentation and Accessories).
37

Analytical instruments that communicate with the Agilent ChemStation via

GPIB require a GPIB board to be installed in your computer. You can use an
Agilent 82350A, the Agilent 82350B PCI high-performance GPIB interface card
or the Agilent 82357A USB-GPIB Interface.
Instrument Compatibility Matrix

The following table helps you to select the correct interface for your system.
NOTE
The HP 82335 and the Agilent 82341C/D cards are not supported. The Agilent 82350A/B
cards are supported on Windows XP Professional, for HP 1090 Systems only. Note that
Agilent 1100/1200 and 35900E using GPIB communication are not supported with
ChemStation Rev. B.03.0x; LAN connection is required. The upgrade from GPIB to LAN
connection needs to be done PRIOR the upgrade to ChemStation Rev. B.03.0x.
Table 5
NOTE
38
GPIB and Analytical Hardware Compatibility Matrix
Instrument Type
Agilent GPIB Card 82350

A/B
Agilent USB-GPIB
Interface 82357A
HP 1090 LC
Yes
Yes
Agilent 1100/1200LC
No
No
HP 1046 FLD
Yes
Yes
HP 1049 ECD
Yes
Yes
35900E A/D Converter
No
No
The GPIB board is static sensitive and should be handled with care so as not to damage it.
Be sure to hold the board by the edges and do not touch the electrical components. Use
anti-static equipment, such as a grounding mat and wrist-straps, during installation.

Agilent 82350 A/B GPIB Interface card

The Agilent 82350 is a PCI GPIB interface card. Therefore no additional
settings such as changing the I/O base address are required.
Installing a GPIB Interface Board in the PC

Since these boards can be installed in several different computers, the
following instructions are general. If you have difficulties during the
installation, consult your computer manual or dealer.
WA R N I N G
Be careful with the high voltage power supplies.

It may harm your health.
Turn OFF and unplug your computer and all attached electrical devices before
removing any covers.
1 Turn OFF and unplug your computer, then remove the computers cover(s).
2 Select any empty PCI slot to install your 82350 GPIB board. It is best not to
place the GPIB board next to a graphics adapter. As the GPIB connector is
wider than a typical PC slot, you may have to leave the slot(s) adjacent to
the GPIB interface empty.
3 Loosen the mounting screw and remove the selected empty slots rear plate.
4 Holding the board by its edges, insert the board into its slot. Make certain
that the boards edge connector is fully seated. Lock the board into place
with the mounting screw.
5 Replace the computer's cover(s). Plug in, and restart the computer.
GPIB Cabling
When connecting GPIB devices together, there are several basic rules that
should be observed.
1 Whenever possible, turn off and unplug the computer and all attached
devices -before the GPIB cables are installed.
39

2 Before connecting any analytical instrument to a GPIB cable, consult the
documentation supplied with each device and determine its GPIB address.
No two devices connected to the Agilent ChemStation may have the same
address. Alter their addresses as necessary to avoid any duplication. Write
down each GPIB address. This information will be needed later.
3 Try to use short GPIB cables that are two meters long or less.
GPIB cable (0.5 m) (10833D)
GPIB cable (1.0 m) (10833A)
GPIB cable (2.0 m) (10833B)
GPIB cable (4.0 m) (10833C)
4 Connect one end of a GPIB cable to the computers GPIB connector.
5 Be certain that you properly tighten all GPIB connectors. A poor connection
causes errors which are difficult to diagnose.
WA R N I N G
According to the IEEE 488 specification for the General Purpose Instrument Bus
(GPIB), the bus is not designed for dynamic configuration.
Cycling power on a GPIB instrument that is connected to the bus while other GPIB
instruments are actively communicating to the GPIB controller can induce electrical
spikes that may potentially corrupt the GPIB protocol. In extreme cases, this may
require cycling power on all instruments, including the GPIB controller (i.e. typically
the Agilent ChemStation).
Connect GPIB devices in a chain.
6 Connect GPIB devices in a chain. A chain occurs when a GPIB device is

connected to the next GPIB device, and it is, in turn, connected to the next,
and so on. Avoid star configurations (connecting all of the devices to a
central point).
40

Configuring the Agilent 82350 GPIB Interface Drivers

After installing the GPIB board in the computer, it is necessary to install the
corresponding driver and configuration software, located on the Agilent
ChemStation CD-ROM (CD 1 - Installation) in the \IO Libs directory. An IO
Libs installing guide can be found on the CD-ROM (CD 1 - Installation) as well.
To install the I/O Libraries proceed with the following steps:
NOTE
The Agilent ChemStation B.03.01 and greater requires version M.01.01 of the I/O Libraries.
These libraries are supported under Windows XP.
1 Using the Windows Explorer, locate and start m0101.exe in the \GPIB
directory of your Agilent ChemStation CD-ROM (CD 1 - Installation). Follow
the instructions in the installation Wizard (see Figure 14 on page 41). This
will install the Standard Interface Control Library (SICL) on your
computer, update the registry and add a new Program Group called
Agilent I/O Libraries.
The InstallShield Wizard opens and starts the IO Libraries installation.
Figure 14
Install Shield Wizard
41

2 Choose Next/Yes to accept the License Agreement and to view the Readme
Information. With the next step the Selection Screen for the Installation is
coming up.
3 Choose Full installation for installing the SICL libraries and VISA components
(see Figure 15 on page 42). Select Next and the system will start the
installation of the SICL and VISA components of the I/O Libraries.
Figure 15
Choose the full SICL and VISA installation.
4 After the installation has been successfully finished, select Run IO config to
configure the IO interface card.
5 From the list of available interface types choose the correct interface type:
82350 PCI GPIB for the Agilent 82350 board (see Figure 16 on page 43).
42

Figure 16
Selecting the appropriate interface type in the I/O Config Screen
6 Select Configure. The configuration utility, IOCFG.EXE, now attempts to

detect the selected GPIB interface in the PC in order to assign the correct
resources to the card.
NOTE
If the GPIB card is not detected in your PC although it is installed, proceed with the section
Troubleshooting GPIB Interface Problems on page 107. Or refer to the Installation
Instructions coming along with the Agilent ChemStation CD-ROM (CD 2 - User
7 Set the SICL Interface Name to hp82341, the Bus Address to 30 and the
System Controller flag as shown in Figure 17 on page 44. Please note that
the appearance of the dialog slightly differs depending on the type of GPIB
board installed.
43

Figure 17
NOTE
Configuring the GPIB Interface for Use with the Agilent ChemStation
You need to type in the SICL interface name hp82341 with lower case letters.
8 Click OK. The configured GPIB board should now be listed under
Configured Interfaces in the Interface Configuration Screen (Figure 18 on
page 45).
NOTE
44
In order to be recognized by the Agilent ChemStation the SICL Interface Name has to be
hp82341 even if you have an Agilent 82350 GPIB Interface Board installed.

CAUTION
If you replace an Agilent 82350A board with an Agilent 82350B board or vice versa first
remove the configuration using the I/O Config Utility from the I/O Libraries Program
Group before exchanging the board.
After the replacement you must rerun the IOCFG.EXE utility to configure the
correct drivers for the installed board.
Do not attempt to start the I/O Config Utility or Visa Assistant while the
ChemStation is running.
9 You have now successfully configured the GPIB Interface board. Restart the
computer to finish the installation.
Figure 18
The Interface Configuration Screen now shows the configured GPIB Board
The interface configuration screen can be accessed from the I/O Libraries
Program Group by selecting I/O Config.
45

GPIB board Configuration with the Agilent ChemStation

Use the following values to configure the Agilent 82350 GPIB interface for use
with the Agilent ChemStation. The available fields depend on the type of
interface installed. See Figure 17 on page 44.
SICL Interface
Name
The SICL Interface Name is the symbolic name used by the Agilent
ChemStation to identify and address this GPIB interface. The default Interface
Name used by the Agilent ChemStation is hp82341, without the quotes. The
same symbolic name has to be used for both interface types. It needs to be
typed in lower case.
VISA Interface
Name
The default VISA Interface Name is GPIB0 (this setting is not used by the
Agilent ChemStation and thus does not need to be altered).
Logical Unit
The Logical Unit number is used by the Standard Interface Control Library
(SICL) to uniquely identify this GPIB interface. The Logical Unit number is an
integer in the range of 0-10000. The Agilent ChemStation always uses the
default value of 7.
Bus Address
The Bus Address determines the address of this GPIB interface on the GPIB
bus. The Agilent ChemStation uses Bus Address 30 for the System
Controller.
System Controller
The System Controller determines which bus devices talk and listen. Each
device connected to the GPIB bus must have a unique GPIB bus address and
one (and only one) device acting as the System Controller. In Agilent
ChemStation installations, the System Controller is always the GPIB board
installed in the PC.
Configuring and Installing the Agilent 82357A USB - GPIB Interface

and Driver under Windows XP Professional
The USB-GPIB Interface driver (IO libraries) needs to be installed prior to the
connection of the Interface device.
1 Using the Windows Explorer, locate and start m0101.exe in the \IO_Libs
directory of your ChemStation CD-ROM (CD1 Installation). Follow the
instructions in the installation Wizard. This will install the Standard
Interface Control Library (SICL) on your computer, update the registry and
add a new Program Group called Agilent I/O Libraries.
46

The InstallShield Wizard opens and starts the IO Libraries installation.
Figure 19
Install Shield Wizard
2 When the title screen appears, click Next to go the License Agreement
screen and then click Yes to accept the licenses terms and to display the
Readme Information.
Figure 20
Installation screen IO Libraries
3 Click Next to display the Select Type of Installation screen.
47

4 Choose installation option Full Installation, for an installation of both the
SICL libraries and VISA components. The System will go to the next screen
and display the current settings (see Figure 21 on page 48).
Figure 21
Installation options
5 By default SICL is installed in C:\Program Files\Agilent\IO libraries. VISA

is installed by default in C:\Program Files\VISA\winnt. Click Next if you
accept the default setting, or go back to set the directory you want via
Custom Installation option. By clicking Next the IO Libraries will be
installed.
6 The IO Libraries have been successfully installed now. Finish the
installation without viewing the Readme file and without running the IO
Config. Leave the screen with Finish and reboot the system.
7 Plug the 82357A USB-GPIB Interface into the GPIB connector of the GPIB
card of the instrument. If a GPIB cable is used to connect multiple modules
(e.g. HP 1090 mainframe and DAD), the USB Interface device needs to be
plugged into the GPIB connector (see Figure 22 on page 49).
48

Figure 22
82357A USB-GPIB Interface on e.g. HP 1090/DAD
8 Connect the USB connector of the 82357A USB-GPIB interface into the USB
port of your Computer. The hardware wizard detects a USB device and the
Found New Hardware Wizard will start (see Figure 23 on page 50).
9 Select Option 1 and click Next and the installation will start (see Figure 23 on
page 50).
49

Figure 23
Found New Hardware Wizard Windows XP, first detection
10 To initialize the interface select Option 1 and click Next and the initialization
will start (see Figure 24 on page 51).
50

Figure 24
Found New Hardware Wizard Windows XP, first detection
11 The system will initialize the USB device. This might take some seconds (see
Figure 25 on page 52).
51

Figure 25
Initializing the 82357A USB-GPIB Interface in Windows XP, first detection
12 Select Finish to close the wizard after the initialization Hardware driver(see
Figure 26 on page 53). The initialization of the USB device is finished, the
installation still needs to be done.
52

Figure 26
Finish Device Driver Installation in Windows XP
13 The hardware wizard detects now the USB-GPIB device for a second time
and the Found New Hardware Wizard starts again (see Figure 27 on page 54).
14 Select Option 1 and click Next (see Figure 27 on page 54).
53

Figure 27
Found New Hardware Wizard Windows XP, second detection
15 Select Option 1 and click Next to start the installation (see Figure 28 on
page 55).
54

Figure 28
Found New Hardware Wizard Windows XP, second detection
16 The system initializes the 82357A USB-PGIB Interface device. This might
take some seconds (see Figure 29 on page 56). Please wait until the
configuration screen appears, see step 14.
55

Figure 29
Installing the 82357A USB-GPIB Interface in Windows XP, second detection
17 The system automatically detects the installed USB-GPIB Interface. Select

Accept to start the IO Configuration Utility (see Figure 30 on page 57).
56

Figure 30
USB- GPIB Interface detected
18 Set the SICL Interface Name to hp82341 and the Bus Address to 30 as shown
in Figure 31 on page 58. Press OK to finish the configuration.
57

Figure 31
NOTE
Configuring 82357A USB-GPIB Interface
You need to type in the SICL interface name hp82341 with lower case letters.
19 Select Finish to close the wizard after the installation of the Hardware
driver(see Figure 32 on page 59). The installation of the USB device is
finished, the new hardware wizard will show a taskbar message in the right
hand corner of the Windows XP system: Your new Hardware is found and
ready to use.
58

Figure 32
Finish Device Driver Installation in Windows XP
59
60


4 Installing the Analytical Instruments

Connecting an Agilent 1100/1200 Series LC to the
Agilent ChemStation
62
Instrument cabling
62
Connecting Agilent 1100/1200 modules to the

Agilent ChemStation using LAN
63
66
GPIB Cabling
66
USB-GPIB Cabling
67
Remote Cable
67
Setting the GPIB Address
69

70
70
35900E Communication
71
Remote Control Cabling
71
72
1090 and Miscellaneous LC Instrument Firmware Requirements 76

This chapter describes how to connect the HP 1090, HP 1046, HP1049, and
Agilent 1100/1200 LC analytical instruments (and specific detectors) to the
Agilent ChemStation using LAN, GPIB, or USB-GPIB connection. Default GPIB
address settings and firmware requirements for the LC Instruments are also
described.
61

Connecting an Agilent 1100/1200 Series LC to the

Agilent ChemStation
You can connect the following modules of the Agilent 1100/1200 Series LC to
your Agilent ChemStation using LAN:
Agilent 1100/1200 autosampler (ALS)
Agilent 1100/1200 well plate autosampler (WPS)
Agilent 1100/1200 dual loop autosampler (DLA)
Agilent 1100/1200 thermostatted autosampler (ALS)
Agilent 1100/1200 pumping system (PMP)
Agilent 1100/1200 diode array detector (DAD)
Agilent 1100/1200 multi wavelength detector (MWD)
Agilent 1100/1200 variable wavelength detector (VWD)
Agilent 1100/1200 fluorescence detector (FLD)
Agilent 1100/1200 refractive index detector (RID)
Agilent 1100/1200 thermostatted column compartment (TCC)
Agilent 1100/1200 chip cube interface (CC)
Agilent 1100/1200 fraction collector (AS/PS)
Agilent 1100/1200 micro fraction collector (MFC)
Agilent 1100/1200 vaccum degasser
Instrument cabling
Installation and interconnection of your Agilent 1100/1200 Series LC modules
are described in more detail in the Agilent 1100/1200 Series Module reference
manual supplied with each module.
Connect a controller-area network (CAN) cable between each of your
Agilent 1100/1200 modules except the vacuum degasser. One CAN cable is
included with each Agilent 1100/1200 module.
62

Connect a remote cable (part number 5061-3378) between the Vacuum

Degasser and one other Agilent 1100/1200 module.
Connect the Agilent 1100/1200 module with the G1369A LAN card inserted
to your LAN component, using the appropriate EtherTwist 10BaseT cable.
See sections Connecting Agilent 1100/1200 modules to the
Agilent ChemStation using LAN on page 63 for details.
The G1315C DAD-SL or G1365C MWD-SL detectors have on-board LAN
communication, use the appropriate EtherTwist 10BaseT cable. See
sections Connecting Agilent 1100/1200 modules to the
Agilent ChemStation using LAN on page 63 for details.
NOTE
We recommend connecting the LAN card cable to an Agilent 1100/1200 detector. Using
the G1315C DAD or G1365C MWD this is a definitiv must. If no Agilent detector is used,
please contact your Agilent Representative to get information about the insertation point of
the communication card.
Connecting Agilent 1100/1200 modules to the Agilent ChemStation

using LAN
This section gives a brief description on how to set up a dedicated mini LAN
for the analytical instruments. If you are going to connect to an existing LAN,
please contact your system administrator for further assistance.
NOTE
A LAN connection requires the Agilent 1100/1200 modules to have firmware version
A.06.02 or higher. The main board (DAM) of the module with the G1369A LAN card inserted
must be GXXXX-66520 (exchange board GXXXX-69520), were XXXX denotes the part
number of the Agilent 1100/1200 module.
Single instrument installation

In a single instrument installation, only one instrument can be connected to
the PC and the PC can not be connected to a LAN. In this configuration, use
the black 10 ft. long crossover twisted pair cable 5183-4649, supplied with the
Agilent ChemStation software. It can only be used for the direct connection of
a single instrument to the PC.
63

NOTE
For direct single instrument connection you can only use the cross-over cable 5183-4649
supplied with the Agilent ChemStation software. Do not use the standard Ethertwist
10BaseT cable supplied with the G1369A LAN card.
Multiple instrument installation

If you want to connect more than one instrument to the Agilent ChemStation
PC or if the PC needs to connect to a network, a hub (for example the G2402A
12 port hub) is required. The hub G2402A comes with one standard 8m
EtherTwist 10BaseT cable G1530-61480 that is used for the connection to the
PC. Use the standard EtherTwist 10BaseT cable G1530-61480 provided with
the G1369A LAN cards to connect the hub to the instruments.
NOTE
Do not use the cross-over cable 5183-4649 in an installation with multiple instruments.
1 Personal computer
2 Network hub
3 Instruments
Use standard twisted pair LAN
cables for the connections.
Figure 33
64
LAN connection using a hub

Setting the IP Address of the Agilent 1100/1200 module

The IP address can be set using the following options:
The IP address of the G1369A LAN card inserted to the Agilent 1100/1200
module can not be set from the Agilent 1100/1200 system. A BootP Service can
be used, to provide the G1369A LAN card with an appropriate IP address,
each time it is started. Refer to section Installing the Agilent BootP Service
Program on page 35 and section What is the Agilent Bootp Service? on
page 94.
NOTE
You need to ensure that the BootP Service is running before the instrument is switched on,
as the configurational data is only loaded to the G1369A LAN card when the module is
switched on.
Since Revision A.09.1 it is possible to assign fixed IP address to the Agilent

1100/1200 modules, using the Control Module or Telnet to assign IP addresses.
The IP address will be stored in the non-volatile RAM of the modules. The
minimum required firmware for the G1369A LAN card is A.01.05. For more
details, please refer to the manual Agilent G1369A LAN Interface. The manual
is available as PDF document in the manual section of the ChemStation
CD-ROM (CD 2 - User Documentation and Accessories).
65

You can connect the following HP 1090 Liquid Chromatograph components to

your Agilent ChemStation:
HP 1090 LC Mainframe. This includes injector, pump and oven.
HP 1090 diode array detector
Installation of your HP 1090 is described in the Installation Guide contained
in the Guide Books for the HP 1090 (part number 01090-90214).
Starting with B.01.03 it is possible to use eiter the common GPIB connection
or the Agilent 82357A USB-GPIB Interface as replacement. This connection
requires the 82357A USB-GPIB Interface and a USB port on your PC.
GPIB Cabling
1 Connect an GPIB cable from the GPIB connector at the rear of the HP 1090
to the GPIB connector on the diode array detector (DAD).
2 Connect an GPIB cable from the GPIB connector on the DAD to the GPIB
connector on your computer.
The following GPIB cables are available:
Part Number
Length
10833A
1m
10833B
2m
10833D
0.5 m
A 10833B cable is included with your HP 1090 Series II system and a 10833D
cable is included with your HP 1090 DAD.
66

USB-GPIB Cabling
1 Connect an GPIB cable from the GPIB connector at the rear of the HP 1090
to the GPIB connector on the diode array detector (DAD).
2 Plug the USB-GPIB Interface on top of the GPIB connector on the DAD to
the USB port on your computer.
For details please refer to Configuring and Installing the Agilent 82357A USB
- GPIB Interface and Driver under Windows XP Professional on page 46.
Remote Cable
Each analytical component of the system must be connected with a remote
cable (part number 01040-60202) so that time-critical events, such as
injections, can be coordinated. For example, with an HP 1090 system fitted
with a diode array detector (DAD), you must connect a remote cable between
the DAD and the rear of the HP 1090.
NOTE
A remote control cable (part number 01040-60202) and an GPIB cable (part number
10833B) are included with the diode array detector when ordered as an option to an HP
1090 system.
Some typical HP 1090 system configurations are shown in Figure 34 on

page 67 and Figure 35 on page 68.
Figure 34
HP 1090 Win Liquid Chromatography System
1 HP 1090 Series II/M

2 Remote cable (01040-60202) included with HP 1090M #083
67

3 10833D GPIB Cable (0.5 m) included with HP 1090M #083
4 Agilent ChemStation
5 Centronics cable
6 Printer
7 10833B GPIB Cable (2 m)
Figure 35
HP 1090 Win LC System (Includes FLD and A/D Converter)
1 APG remote cable (5061-3378)

2 Remote cable (01040-60202) included with HP 1090M #083
3 HP 1090 Series II/M
4 10833D GPIB Cable (0.5 m) included with HP 1090M #083
5 10833B GPIB Cable (2 m) included with Agilent ChemStation
6 Agilent ChemStation
7 Centronics cable
8 Printer
9 Standard Twisted Pair Ethernet Cable
10 Two signal cables (option to Agilent 35900E)
11 10833A GPIB Cable (1 m) included with FLD #305
12 HP 1046A Fluorescence Detector
13 APG remote cable included with FLD
14 Agilent 35900E Dual-Channel Interface
68


These instruments are shipped with the default GPIB addresses listed in
Table 6 on page 69.
Table 6
Miscellaneous Instrument Default GPIB Addresses
LC Module
Default GPIB Address
LAN Connection
HP 1046 FLD
12
--
HP 1049 ECD
11
--
Agilent 35900E Dual Channel

Interface
--
defined IP address
If you configure more than one module of the same type, you must change the
default settings so that each module has a unique GPIB address. For further
details, refer to the instrument handbooks.
69

Connecting Miscellaneous Instruments to the

Agilent ChemStation
In addition to the HP 1090 and Agilent 1100/1200 Series, you can connect the
following stand-alone analytical instruments to your Agilent ChemStation:
Agilent 35900E dual channel interface
HP 1046 fluorescence detector
HP 1049 electrochemical detector
To install these instrument, see the operators handbook shipped with each
instrument.

These instruments are shipped with the default GPIB addresses listed in
Table 6 on page 69.
Table 7
Miscellaneous Instrument Default GPIB Addresses
LC Module
Default GPIB Address
HP 1046 FLD
12
HP 1049 ECD
11
Agilent 35900E Dual

Channel Interface
13
default settings so that each module has a unique GPIB address. For further
details, refer to the instrument handbooks.
70

35900E Communication
These instruments are using LAN connection for communication, the setup is
similar to the LAN set up for LC instruments. Please see the operators
handbook shipped with the instrument.
default settings so that each module has a unique IP address. For further
details, please refer to the instrument handbooks.
Remote Control Cabling

All modules of an instrument that accept timetable entries or which go into
the run mode during an analysis must be included in the remote control loop.
In general, each module must be connected to the other devices with a remote
control cable. The HP 1046 FLD, the 1049 ECD, and the HP 1090 Series II
include a dual-APG remote connector with pass-through capability. This
means that you may use one remote connector as an input and the other as an
output. All remote signals which enter these connectors pass through to the
other connector, as well as being seen by the module.
The 35900E dual channel interface use APG remote, but the two remote
connectors are not pass-through connections. When you operate the 35900E
with both channels, only the A-channel remote will be connected. The
B-channel acts in synchronization with the A-channel and will react to the
remote signals from the A-channel remote connector. The only time you
connect a remote cable to the B-channel remote connector is if you wish to
operate the B-channel independently. In this mode, one instrument may start
and stop the A-channel while another instrument has control of the B-channel.
The built-in DAD of the HP 1090 Series I LC does not include standard APG
remote connections. Anyhow the HP 1090 I LC needs to be updated to an
Series II LC.
When connecting additional devices (that typically support APG-remote) to an
HP 1090 system, a signal distribution module must be used.
71

The LC ChemStation software will require minimum firmware revisions in

order to work with the following devices shown in Table 8 on page 72.
NOTE
Table 8
Using 1100/1200 modules in one instrument, the firmware of the whole module stack
needs to rund on the minimum required firmware mentioned in the table below. Mixed
firmware versions are not supported.
LC Instrument
Product Number
Firmware Revision
Part Number
Agilent 1100/1200 Automation Interface
G2254A
A.06.02 or newer
n.a.1
Agilent 1100 Autosampler
G1313A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Thermo. Autosampler
G1329A
A.06.02 or newer
n.a.1
Agilent 1200 Thermo. Autosampler SL
G1329B
A.06.04 or newer
n.a.1
Agilent 1100 Micro Sampler
G1389A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Preparative Autosampler
G2260A
A.06.02 or newer
n.a.1
Agilent 1100 Well Plate Autosampler
G1367A
A.06.02 or newer
n.a.1
Agilent 1200 High Performance Autosampler
G1367B
A.06.02 or newer
n.a.1
Agilent 1200 High Performance Autosampler SL
G1367C
A.06.02 or newer
n.a.1
Agilent 1100 Thermo. Well Plate Autosampler
G1368A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Micro Well Plate Autosampler
G1377A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Thermo. Micro Well Plate Autosampler
G1378A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Dual Loop Autosampler PS
G2258A
A.06.02 or newer
n.a.1
Samplers
72

LC Instrument
Product Number
Firmware Revision
Part Number
Agilent 1100/1200 Thermostated Column Compartment
G1316A
A.06.02 or newer
n.a.1
Agilent 1200 Thermostated Column Compartment SL
G1316B
A.06.02 or newer
n.a.1
Agilent 1100/1200 Chip Cube Interface
G4240A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Isocratic Pump
G1310A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Quaternary Pump
G1311A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Binary Pump
G1312A
A.06.02 or newer
n.a.1
Agilent 1200 Binary Pump SL
G1312B
A.06.02 or newer
n.a.1
Agilent 1100/1200 Capillary Pump
G1376A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Preparative Pump
G1361A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Nano Pump
G2226A
A.06.02 or newer
n.a.1
Agilent 1100 DAD
G1315A
A.06.02 or newer
n.a.1
Agilent 1100/1200 DAD
G1315B
A.06.02 or newer
n.a.1
Agilent 1100/1200 DAD SL
G1315C
B.01.02 or newer2
n.a.1
Agilent 1200 DAD
G1315D
B.01.04 or newer3
n.a.1
Agilent 1100 MWD
G1365A
A.06.02 or newer
n.a.1
Agilent 1100/1200 MWD
G1365B
A.06.02 or newer
n.a.1
Agilent 1100/1200 MWD SL
G1365C
B.01.02 or newer2
n.a.1
Agilent 1200 MWD
G1365D
B.01.04 or newer3
n.a.1
Agilent 1100/1200 FLD
G1321A
A.06.02 or newer
n.a.1
Agilent 1100 VWD
G1314A
A.06.02 or newer
n.a.1
Agilent 1200 VWD
G1314B
A.06.02 or newer
n.a.1
Column Compartments
Pumps
Detectors
73

LC Instrument
Product Number
Firmware Revision
Part Number
Agilent 1200 VWD SL
G1314C
A.06.02 or newer
n.a.1
Agilent 1100/1200 RID
G1362A
A.06.02 or newer
n.a.1
Agilent 1100/1200 UIB
G1390A
A.06.02 or newer
n.a.1
Agilent 1100 Fraction Collector
G1364A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Fraction Collector PS
G1364B
A.06.02 or newer
n.a.1
Agilent 1100/1200 Fraction Collector AS
G1364C
A.06.02 or newer
n.a.1
Agilent 1100/1200 Micro Fraction Collector
G1364D
A.06.02 or newer
n.a.1
2Pos/10Port Valve
G1157A
A.06.02 or newer
n.a.1
2Pos/6 Port Valve
G1158A
A.06.02 or newer
n.a.1
2Pos/6 Port Valve SL
G1158B
A.06.02 or newer
n.a.1
6Position Selection Valve
G1159A
A.06.02 or newer
n.a.1
12Pos/13 Port Selection Valve
G1160A
A.06.02 or newer
n.a.1
2Pos/6 Port Micro Valve
G1162A
A.06.02 or newer
n.a.1
2Pos/10 Port Micro Valve
G1163A
A.06.02 or newer
n.a.1
Agilent 1100/1200 Degasser
G1322A
All Revisions
Agilent 1100 Micro Degasser
G1379A
All Revisions
Agilent 1200 Micro Degasser
G1379B
All Revisions
Agilent 1100/1200 manual injector
G1328B
All Revisions
Agilent 1100/1200 Thermostat f. Sampler/Frac.Collectors
G1330B
All Revisions
G1330B
All Revisions
Fraction Collectors
Valves
Others
74

LC Instrument
Product Number
Firmware Revision
G1330B
All Revisions
G1330B
All Revisions
Agilent 1100 Control Module
G1323A
A.02.104
n.a.1
Agilent 1100/1200 Control Module
G1323B
B.04.025
n.a.1
Agilent 1100/1200 Instant Pilot
G4208A
B.01.02 or newer
n.a.1
Part Number
1 The Agilent 1100/1200 series of LC modules have flash ROM memory. Firmware updates are distributed electronically. The
latest firmware can be downloaded from the following Agilent Technologies website: http://www.chem.agilent.com/scripts/cag_firmware.asp. Starting with firmware A.06.0x/B.01.0x, a new firmware update tool is available and
distributed along with the Agilent ChemStation Software CD-ROM (CD 1- Installation).
2 The Agilent G1315C DAD and G1365C MWD modules require minimum firmware B.01.02. This firmware is only compatible
with firmware A.06.02 or higher. As soon as a G1315C/G1365C is used in an 1100/1200 stack, the whole stack needs to be
compatible using firmware A.06.02 or higher.
3 The Agilent G1315D DAD and G1365D MWD modules require minimum firmware B.01.04. This firmware is only compatible
with firmware A.06.02 or higher. As soon as a G1315D/G1365D is used in an 1100/1200 stack, the whole stack needs to be
compatible using firmware A.06.02 or higher.
4 The Agilent 1100 local user interface G1323A is only supported in combination with the following modules: G1310A,
G1311A, G1312A pumps, G1313A ALS, G1314A VWD,G1315A DAD, G1316A TCC, G1321A FLD
5 The Agilent 1100/1200 local user interface G1323B is not supported in combination with a 1100/1200 stack that includes
a Chip Cube module G4240A
75

The LC ChemStation software will require minimum firmware revisions in

order to work with the following devices shown in Table 9 on page 76.
Table 9
LC Instrument
1090 and miscellaneous LC Instrument Firmware Requirements

Product Number
Firmware Revision
Part Number
HP 1090L
MEM D-3321
01090-66573
HP 1090L
LIB E-3321
01090-66579
HP 1090M
MCO all revisions
01090-66542
HP 1090M
MEM D-3321
01090-66573
HP 1090 II/L
LIB E-3321
01090-66579
HP 1090 DAD
option #080
COM board firmware rev.E-3014
79880-13007
HP 1046 dual grating FLD
HP 1046A
FDC board rev. B-2630
01046-66503
HP 1046 dual grating FLD
HP 1046A
FDI board with firmware rev. B or C
01046-69509
HP 1049 Electrochemical Detector
HP 1049A
Firmware rev. E-3314
01049-13002
76
NOTE
If there is a CTL board in the HP 1090, replace it with the assembly kit MCO/MEM board
01090-69708.
NOTE
Pre-1985 HP 1090 LCs require the following new boards: COM board (79880-69516) and
DAP board 79880-69504.
NOTE
When connecting an HP1046 and /or a 35900E A/D dual channel interface to an HP 1090M
or HP 1090L (that is, prior to Series II), use the cabling kit 01090-68700 (includes signal
distribution module 01090-60302, two remote cables 01040-60202).

5 Checking Out the

Agilent ChemStation
78
Overview
78
Installation Qualification
78
Adding the Agilent ChemStation Installation Verification Utility79

Agilent ChemStation Installation Qualification Utility Execution
79
Agilent ChemStation IQ Utility Results
79
Generating an Installation Verification Reference File on a

Customized Installation
81
83
Overview
83
Performing the Verification Test
83
Acceptance Criteria
83
This chapter describes how to perform a short system integrity test. The steps
outlined in the chapter help you when validating your system intallation.
77

Overview
After installing the Agilent ChemStation system software on your computer
and configuring the analytical system, your internal validation procedure may
require you to assess the correctness and completeness of the installation and
to verify that the analytical system is fully operational.
Installation Qualification
The Agilent ChemStation Installation Verification utility uses factory
delivered installation reference files to verify the existence, correctness, and
integrity of the required Agilent ChemStation system files (executable
program files, binary register files, macro files, initialization files, help files,
customized report templates). This process is called Installation Qualification
(IQ).
File integrity is verified by comparing the 32-bit cross-redundancy-check
(CRC) checksum of the installed file with the checksum of the original file
recorded on Agilent Technologiess installation master. The file details of the
installation master are delivered on so-called reference files. Modified or
corrupted files have different checksums and are thus detected by the IQ
utility.
The integrity of the reference files themselves is also tracked with the help of
checksums. In case the installation verification utility is supplied with a
reference file that was modified after its generation, this will be flagged in the
report (section invalid reference files).
NOTE
78
Any patches or other changes delivered by Agilent Technologies are not delivered with new
reference files. An IQT Patch Wizard is included in the patch distribution and provides IQ
possibility for the installed system

Adding the Agilent ChemStation Installation Verification Utility

The Agilent ChemStation Installation Verification is automatically installed
and the appropriate IQ reference files are copied to your PCs hard-disk and
the appropriate Agilent ChemStation IQ icons are added to your Agilent
ChemStation group.
Agilent ChemStation Installation Qualification Utility Execution

Double-click on the Installation Qualification icon in the
Agilent ChemStation group.
NOTE
Make sure the Agilent ChemStation is closed before running the Installation Verification
Utility.
The checksum calculation for all Agilent ChemStation system files may take
several minutes. A dialog box indicates the name of the system file that is
currently being analyzed.
Figure 36
Analyzing file
Agilent ChemStation IQ Utility Results

The Agilent ChemStation Installation Verification utility displays the
verification results on the screen and can optionally generate a report of its
findings.
79

On a complete and consistent installation, the installation verification
completes without any error messages and no files are reported missing or
modified.
If the verification report lists files as missing or modified, the installation
verification utility displays the error message Installation Verification
completed with error(s) in the message line.
The Agilent ChemStation Installation Verification reports the following file
categories:
Figure 37
Example for System Modifications Detected by Installation Verification
The printed report contains the license registration numbers used on the
system that was checked. It also lists the reference files (name and date code)
that were used for the installation verification.
Example report:
ChemStation Installation Verification
Installed licenses
80

XM00000000
Reference files
e.g. sys\lc3d.ref (29. May 2004, 12:43)
Identical files
(...)
hpced02.exe 1.1.5.1 238B APG Configuration Editor
hpfabs00.dll 1.0.3.3 9BC0 support functions
(...)
Generating an Installation Verification Reference File on a

Customized Installation
The Agilent ChemStation has an open architecture that allows knowledgeable
users to add their own customization code. In regulated environments, it is
general practice to document such adaptations, additions, or modifications.
For your own tracking purposes, you can use the Agilent ChemStation
installation verification utility to generate a reference file listing for the
customized system. You can use this reference file to qualify the completeness
and integrity of the customized Agilent ChemStation and use it as a tracking
document for future modifications. The program scans for files with the
extensions {*.dlc, *.dll, *.drv, *.enu, *.exe, *.hlp, *.ini, *.mac, *.mcx}.
For example, in order to create the file contents list along with the
corresponding file checksums for your existing installation, execute the
Agilent installation verification utility with the following command line
options:
C:\CHEM32\SYS\HPVERI00.EXE -f custom.ref
Add the resulting reference file to your project documentation. To
automatically run an installation qualification test using the new reference
file, set up an icon with the following command line specification in its
properties:
hpveri00.exe -r custom.ref
81

NOTE
82
See the installation verification help system for a complete description of the installation
verification utilitys command line options. The installation verification does not check
method files, sequence files, or data files. These files are stored in Agilent Technologies
proprietary, unalterable binary formats and are checked for integrity when loaded into the

Overview
The Agilent ChemStation provides a verification test function that checks out
the systems software operation. Using either Agilent-supplied or
user-generated data files and methods, the system will match calculated
integration and quantification results with ones that have been previously
generated and archived in an unalterable, checksum-protected, binary register
file.
When using an Agilent 1100/1200 Series detector, or Agilent CE instrument
with a built-in DAD (G1600A), the verification test can be extended to include
a data acquisition check. The verification test generates a report that includes
a list of the tested configuration, the verification test details like the data file
and method used, and the test results that indicate whether the system passed
a specific test step or not.
Performing the Verification Test

After installing the Agilent ChemStation and successfully passing the
Installation Verification, start the Agilent ChemStation.
Select Run Test from the VerificationView and run the verification test
procedure called default.val.
Please refer to the analytical tasks section of online help system if you
require further information.
Acceptance Criteria
The test was passed successfully, if the verification test report indicates that
all individual test steps were passed. This proves that internal components
such as the Command Processor, Method Handler, the internal Memory
83

Manager for data objects, registers and tables, the Data Analysis modules for
peak identification, peak quantification, report formatting, and the
Agilent ChemStation print spooler are fully operational.
If the test fails, the verification test report indicates which part of the test did
not meet the acceptance criteria. Verify you used the correct verification
procedure. If the verification test continues to fail, we recommend that you
reinstall the Agilent ChemStation. Do not delete your current system
installation, as the installation procedure will copy the original
Agilent ChemStation files on top of the existing ones.
84

6 Configuring the Analytical System

86
What is the Agilent ChemStation Configuration Editor?
86
Starting the Configuration Editor
86
What You Do
87
Select and Configure the GPIB Interface
87
Configuring Paths
88
90
Configuring an Agilent ChemStation Instrument
90
Configuring an Agilent 1100/1200 Series LC System
90
Configuring an HP 1090 LC System
93
94
What is the Agilent Bootp Service?
94
Addresses
95
Using the Agilent Bootp Service Program
95
Configure the Agilent Bootp Service Program
96
Determine your MAC Address
96
Using the Launch Manager
97
Configure the Agilent Bootp Service
99
Configuring the G1369A LAN Card Using BootP
100
The first section in this chapter describes how to configure the communication
of your Agilent ChemStation software with your LC instruments using a GPIB
or LAN connection with the Agilent ChemStation Configuration Editor. The
second section describes how to configure a BootP Service that can be used for
communication using a LAN connection.
85

What is the Agilent ChemStation Configuration Editor?

The Agilent ChemStation Configuration Editor is a program that allows the
easy configuration of your Agilent ChemStation software. This includes
Detecting the GPIB interface in your PC,
Selecting communication using LAN or GPIB (as well for USB-GPIB),
Configuring the analytical hardware connected to the PC,
Configuring the path used for method, data and sequence storage,
Configuring color display of the Agilent ChemStation.
After installing the Agilent ChemStation software, you are prompted to
configure the complete analytical system.
The Agilent ChemStation Configuration Editor needs to be run every time a
GPIB device is connected to or disconnected from the GPIB bus of the PC.
Since the Configuration Editor modifies the internal communication module
database, the Agilent ChemStation must be closed beforehand.
For a LAN connection, the configuration editor only needs to be run, if the IP
address of the instrument is changed.
Starting the Configuration Editor

CAUTION
Any devices that contain an GPIB interface and do not have the power on cannot
communicate with the Agilent ChemStation.
The Agilent ChemStation reports a Power Fail event on the corresponding device.
To avoid communication errors on the GPIB bus, we strongly discourage you from
powering modules on or off while the Agilent ChemStation is running.
86

NOTE
If GPIB connection is used, a GPIB interface must be installed in the PC or a USB port for
the USB-GPIB interface device must be present. This does not apply for the Agilent
ChemStation Data Evaluation Only products, Agilent G2090BA and Agilent G2190BA. The
I/O Config utility IOCFG.EXE must have been run to configure the interface.
NOTE
If LAN connection is used, Microsoft TCP/IP protocol must be installed and configured on
the Agilent ChemStation PC. In addition a BootP Service must be running and configured
for the instruments to be used.
1 Start the Agilent ChemStation Configuration Editor by double-clicking on

the Configuration Editor icon in the Agilent ChemStation start-up group or
by running C:\CHEM32\HPCED02.EXE from the Start/Run menu .
What You Do
1 Select/configure the GPIB interface (for GPIB, USB-GPIB communication)
or set up the BootP Service or fix IP address in module (for LAN
communication)
2 Configure the instrument type (1090 system, modular system, data analysis
only, all with or without spectral capabilities).
3 Configure the instrument devices, like special detectors, access points, etc.
4 Configure the paths used for methods, sequences, and data files (optional).
5 Configure the colors used for chromatograms, titles, and baselines
(optional).
6 Save your configuration.
Select and Configure the GPIB Interface

To establish communication through the GPIB bus (either using GPIB cards or
USB-GPIB Interface), the interface configuration must be set in the
Configuration Editor. These settings are not necessary if only a LAN
connection is used.
87

We recommend that you check your GPIB card has the correct default settings.
SeeGPIB and USB-GPIB Communication on page 38 for more information.
1 Choose GPIB Card USB-GPIB Interface from the Configure menu. The
Configuration Editor attempts to detect the installed card.
2 For the Agilent 82350A , Agilent 82350B GPIB interface as well for the
Agilent 82357A USB-GPIB Interface, select hp82341.
3 Choose OK to save changes and return to the Configuration Editor display.
Details are outlined in the manual Agilent IO Libaries . The manual can be
found on the Agilent ChemStation CD-ROM (CD 2 - User Documentation and
Accessories) in the manual section, Installation.
Configuring Paths
The Configuration Editor lets you specify alternate path settings for your
sequences, methods, and data files. This allows storing data files on a separate
drive. Starting with B.02.01 it is as well possible to configure the path for
methods, sequences, and data files within the Agilent ChemStation using the
Preferences.
This section describes how to configure different paths for your methods,
sequences, and data files. The AgilentChemStation default path settings are
also listed.
CAUTION
Default method and sequence are used as a template for new sequences and methods.
You cannot set up new methods and sequences anymore.
Do not delete the default method and sequence (DEF_XX.S and DEF_XX.M,
where XX stands for the technique (LC, CE or GC ).
NOTE
Before you modify the path settings using the Configuration Editor, you need to create the
appropriate directories.
1 Choose Paths from the Configure Menu.

2 Set your data file path. Default = C:\CHEM32\1\DATA\
88

3 Set your method path. Default = C:\CHEM32\1\METHODS\

4 Set your sequence path. Default = C:\CHEM32\1\SEQUENCE\
5 Choose OK to update the path information and return to the Configuration
Editor display.
NOTE
All path names have to be terminated with a backslash \.
89

Configuring an Agilent ChemStation Instrument

In most cases, the Agilent ChemStation instrument configuration information
has to be adapted to match the connected analytical hardware after the
installation of the Agilent ChemStation application software. Most Agilent
Technologies analytical hardware is modular and can be combined in various
ways. The following instrument types are available for an LC configuration:
Table 10
LC Instrument Types in the Agilent Configuration Editor
Instrument Type
Supported LC Hardware
Data Evaluation Capabilities
LAN
1090 3D LC system
HP 1090 DAD
spectra evaluation included
No
1090 LC system
HP 1090
no spectral evaluation included
No
LC 3D data analysis only
no instrument control
No
LC data analysis only
no instrument control
no spectra evaluation included
No
Modular 3D LC system
Agilent 1100/1200
Yes
Modular LC system
Agilent 1100/1200
no spectra evaluation included
Yes
Configuring an Agilent 1100/1200 Series LC System

The following example describes how to configure an Agilent 1100/1200 Series
system with modules connected together by the Agilent 1100/1200 Series
controller area network (CAN) that will automatically configure each module .
1 Start the Agilent ChemStation Configuration Editor.
2 Select the Configure/Instruments menu.
3 Select the appropriate instrument type (Modular LC System or Modular 3D LC
System).
4 Assign a name to your analytical instrument.
90

5 Select NO for initially start instrument session. This allows you to start your
instrument using the appropriate Agilent ChemStation icon.
6 Specify your preferred screen window size (Normal,Icon, Full Screen).
7 Click OK.
8 In the Device Configuration dialog, select 1100/1200 System Access from the
list (see Figure 38 on page 91). Select the LAN radio button.
Figure 38
Agilent ChemStation Configuration Editor: Device Configuration Dialog
9 Select Add to add the selected system access to the list of configured
modules. For LAN access type in the IP address (or host name if name
resolution is implemented on your network) of the Agilent 1100/1200
module with the LAN connection.
10 Select OK to exit and return to the Configuration Editor display.
11 Save your configuration changes by selecting File/Save.
12 This step is applicable for online instruments using LAN connection only.
For data analysis only configurations, proceed with the next step. If your IT
department did not set up a BootP Service for you, you need to do this
yourself or you need to fix the IP address with the analytical instrument.
91

13 When you start the Agilent ChemStation for the first time, the Configure
System Access dialog box displays the Agilent 1100/1200 modules detected
during instrument initialization (see Figure 39 on page 92). Agilent
1100/1200 modules that have been recognized are listed as online (indicated
by a green icon) along with their serial numbers. Agilent 1100/1200
modules that have not been detected are marked offline. You can selectively
take certain modules out of the software configuration, e.g. if your
Agilent 1100/1200 system includes two Agilent 1100/1200 detectors and
your next analysis only requires one of them. The unneeded
Agilent 1100/1200 module may then remain turned off but still be
connected. You may also re-order modules, for example if you wish to use
one pump as the primary analytical pump and another as a make-up pump.
After modifying the configuration, you must restart the Agilent
ChemStation. The Configure 1100/1200 System Access dialog is available from
the Instrument menu in the Method and Run-Control view, when Full Menus
are displayed.
Figure 39
92
The Configure LC System Access dialog allows to selectively include detected

Agilent 1100/1200 modules into the configuration.


The following example describes how to configure an HP 1090 system with the
following devices:
HP 1090 PV5 solvent delivery system
HP 1090 autosampler
HP 1090 diode array detector
Configuring Your Instrument Devices

This section describes how to set up each device that you have connected to
build your instrument.
1 Start the Agilent ChemStation Configuration Editor.
2 Select the Configure/Instruments menu.
3 Select the appropriate instrument type (e.g. 1090 3D LC System).
4 Assign a name to your analytical instrument.
5 Select NO for initially start instrument session. This allows you to start your
instrument using the appropriate Agilent ChemStation icon.
6 Specify your preferred screen window size (Normal,Icon, Full Screen).
7 Click OK.
8 Set the GPIB address of your HP 1090 mainframe (default is 14).
9 Select the HP 1090 diode-array detector from the Modules list box.
10 Set the GPIB address in the GPIB address list box (default is 15).
11 Choose Add from the main menu to add the HP 1090 diode array detector
in the Selected Modules list box.
NOTE
If your HP 1090 uses a filter photometric detector (FPD), you must configure an Agilent
35900E dual channel interface to convert the analog detector signals to digital signals for
the Agilent ChemStation.
12 Select OK to exit and return to the Configuration Editor display.

13 Save your configuration changes by selecting File/Save.
93

What is the Agilent Bootp Service?

Agilent BootP Service provides central administration of IP addresses for
Agilent instruments on a LAN. The service runs on the instrument LAN PC,
which must be running TCP/IP network protocol and cannot run a DHCP
server or another BootP Service.
When an instrument is powered on, an Agilent JetDirect card in the
instrument broadcasts a request for an IP address or Host Name and provides
its hardware address as an identifier. The request may continue for up to 5
minutes. The Agilent BootP Service answers this request and passes a
previously defined IP address and Host Name associated with the hardware
address to the requesting instrument.
In case of the instrument on the LAN, the G1369A LAN card used to connect
the Agilent 1100/1200 modules to the LAN does not store any settings, so each
time the instrument is powered on the card sends a request to the network for
an IP address and setting information. The implementation of the bootp
protocol on the G1369A LAN card conforms to RFC 951 and RFC 1048.
In order to provide this information to the card, the BootP Service must be
operational before the card sends the request and the BootP Service must be
configured to know the hardware address (also called MAC address) of the
G1369A LAN card. Multiple G1369A LAN cards can be configured with a
single BootP Service.
If your network is already using a BootP Service, please refer to section
Configuring the G1369A LAN Card Using BootP on page 100. If you do not
have a BootP Service, we suggest to use the Agilent Bootp Service program
supplied on the Agilent ChemStation CD-ROM (CD 1 - Installation).
94

Addresses
Before installing and configuring BootP Service, you must know the IP
addresses of the computer and instruments, the subnet mask, and the
gateway. If the system is on an isolated LAN, the following default addresses
can be used.
Table 11
Example /Default addresses
Device
Address
PC
10.1.1.100
LC - 1
10.1.1.101
LC - 2
10.1.1.102
Subnet mask
255.255.255.0
Gateway
10.1.1.100
Using the Agilent Bootp Service Program

The Agilent Bootp Service program allows configuring the G1369A LAN card.
Within the Launch Manager of the program you can easily add a MAC address
and IP address pair to the configuration. The Launch Manager is displaying a
list of the currently configured MAC address and allows modification of the
current configured settings.
95

Figure 40
Configure the Agilent Bootp Service Program

Before configuring BootP Service, you must know the IP addresses of the
computer and instruments, the subnet mask, and the gateway. The MAC or
hardware address of the G1369A LAN is a world wide unique identifier. No
other network device will have the same hardware address. The MAC address
is printed on the card or can be read out during the configuration procedure:
NOTE
If the PC has been rebooted after installing the Agilent BootP Service, the Agilent Bootp
Service is started automatically during the next startup of the system. To change BootP
settings, the service must be stopped, the changes made, then restarted. The details are
documented within this section.
Determine your MAC Address

1 Determine the MAC address of the LAN card installed. The number is
engraved in the board. Either write down the MAC address before installing
the card into the module. To remove the card from the system, turn the
96

module off before removing the LAN card. Read the MAC address from the
label. Reinstall the card and turn on the module again or
- OR -
use the Agilent BootP Service to determine the MAC address.

2 To obtain the MAC address from the instrument using Agilent BootP
Service you have to power cycle the LC module containing the LAN card.
3 After power cycling the module open the logfile using Notepad. The
contents will be similar to the message in the figure below:
Figure 41
MAC address information in the BootP logfile
4 Record the MAC address, here called hardware address.

5 Close the logfile before turning on another instrument. In order to add an
additional instrument you need to rerun step 1-3 in order to get the MAC
address for the additional instruments.
NOTE
The logfile will not be updated if the file is open.
Using the Launch Manager

1 To add the instrument to the network go to Start > Programs > Agilent BootP
Service > Edit BootP Settings. The BootP Settings screen appears.
2 Uncheck Do you want to log bootp requests?
97

3 Click Launch Manager.
The BootP Manager screen appears.
Figure 42
4 Click Add.... TheAdd Bootp Entry screen appears.
5 Make the following entries for your instrument:
MAC Address
Host Name
IP Address
Comment, if desired
Subnet Mask
Gateway Address
6 Click OK.
7 Exit Launch Manager and power cycle the LC module.
8 Ping the IP address to verify.
98

Configure the Agilent Bootp Service

BootP Service starts automatically when your PC reboots. In order to add an
additional instrument or to change the configuration the service must be
stopped. After the changes have been applied, the service must be restarted.
1 To stop the BootP service go to Start > Settings > Control Panel and select
Administrative Tools > Services. The Service screen appears.
Figure 43
Service screen
2 Right click Agilent BootP Service.

3 Select Stop.
4 Close the Services and Administrative Toolsscreen.
99

5 To edit the BootP Settings go to Start > Programs > Agilent Boot Service > Edit
BootP Settings. The BootP Settings screen appears. When this screen opens
the first time, the default settings from installation are displayed.
6 To edit the TabFile you have to select Maintain bootp tabfile? The default tab
file was created at installation and is located at Program Files\Common
Files\Agilent Shared\BootP\bin\TabFile. It contains configuration
information entered on this screen. If necessary you can
change the Bootptab File Location by using browse on the right (a valid
BootP tabfile must exist).
create your own template for the tabfile by selecting Create template BootP
tabfile? and click Create Template.
7 Check Do you want to log bootp requests?The default logfile was created at
installation and is located at Program Files\Common Files\Agilent Shared\
BootP\bin\LogFile. It contains an entry for every time a device requests
configuration information from BootP. If necessary you can change the
Bootplog File Location by using the browse button on the right (a valid BootP
tabfile must exist).
8 Perform your necessary changes by e.g. creating a logfile entry of a new
instrument and edit the new instrument using the Launch Manager.
9 Uncheck Do you want to log bootp requests?
10 Click OK to save the values or Cancel to discard them. The program ends.
11 To restart the BootP Service go to Start > Settings > Control Panel and select
Administrative Tools > Services. The Service screen appears.
12 Select Start.
13 This completes the configuration.
Configuring the G1369A LAN Card Using BootP

In order for an internal G1369A LAN card to use BootP, the default setting:
bootp=YES must be set. This is the default setting from the manufacturer and
can be viewed on an Agilent 1100/1200 system using one of the
Agilent 1100/1200 control modules. In the System view of the control module,
select Configure > MIO for the module where the G1369A LAN card is inserted
and scroll down to corresponding item.
100

NOTE
When the MIO dialog is opened on the Agilent 1100/1200 control module, the
Agilent ChemStation cannot communicate with the Agilent 1100/1200 system.
To change the setting, the card must be reset; this is done each time the
Agilent 1100/1200 module with the G1369A LAN card inserted is power
cycled.
A BootP Service uses a file called bootptab were all the necessary information
is stored. The BootP Service program on the Agilent ChemStation CD-ROM
(CD 1 - Installation) allows easy editing of the bootptab file. If a BootP Service
is already installed, the following information must be supplied to the system
administrator to be able to correctly set up the bootptab file.
# Bootptab file
# global defaults
global.defaults:\
sm=:\
gw=:
#agilent
LC-1:\
ht=1:\
ha=0060B0047394:\
ip=10.1.1.102:\
sm=255.255.255.255:\
gw=0.0.0.0:\
vm=auto:\
hn:\
bs=auto:\
T145=64:T146=01:T147=01
The entries are described below
101

# LC1100 G1369A LAN Card - Instrument 1
Every entry on a line after a # is a comment and ignored by the BootP
Service.
LC-1:\
Host name of the instrument. The host name communication can only be
used if a DNS server (Domain name server) is correctly set up for the name
or a corresponding entry in the HOSTS file exists (windows\system32\
drivers\etc\hosts).
ha=0060B0047394:\
This is the hardware or MAC address of the G1369A LAN card. It is usually
printed on a label attached to the card. If not, see section Determine your
MAC Address on page 96.
ip=10.1.1.102:\
This is the IP address that will be send to the card. This address needs to be
set in the Agilent ChemStation Configuration Editor.
sm=255.255.255.0:\
This is the subnetmask address that will be send to the card.
gw=
This is the gateway address that will be send to the card.
T145=64:T146=01:T147=01
These parameters set the buffer handling of the G1369A LAN card. These
are required for the instrument on the LAN to work properly and should
not be changed.
Additional entries for the subnet mask and default gateway might be required,
depending on your network setup. These values need to be set by your network
administrator.
102

7 Troubleshooting
105
Power fail reported on the Agilent ChemStation
105
Frequent Buffer Overrun in Instrument Log Book
106
107
Autoadd Button of IOCFG Installs Defaults Incompatible with

the Agilent ChemStation
108
109
An online core must be purchased in order to install additional

instrument modules
109
ChemStation cannot be located on your system. You must first
install Agilent ChemStation before installing an instrument 109
Agilent ChemStation online session does not finish startup 110
key in section [PCS] of CHEMSTATION.INI not valid or
specified file does not exist
112
General Protection Fault in Module...
114
Printing Problems
115
Agilent ChemStation Print Spooler hangs after an error occurred

115
Printing in Multi-Instrument configurations
115
Recovering from Printer hang-ups
116
Printing Messages
117
Computer Problems
119
Sporadic Hang-Ups
119
System Crash in an Online Session
119
Cannot create file
119
Slow Hard-Disk access or Hard-Disk Activity LED is continuously

flickering
120
Agilent ChemStation Performance Degrades over Time
120
121
103
Troubleshooting
Agilent ChemStation does not see an LC hardware modification
(HP 1090)
121
System remains in a wait-state waiting for a module that has
been taken out of the configuration
121
Data File is empty (contains no signal)
122
Devices report Buffer Overflow in the logbook
122
123
What is the WinDebug Utility?
123
WinDebug in Windows XP
123
126
Gathering System Information with Windows XP
126
This chapter will provide elementary troubleshooting information in case

something goes wrong. The chapter will cover the most common software
start-up problems, printer problems and computer problems and how to overcome them.
104
Troubleshooting

If your Agilent ChemStation cannot connect to the analytical instrument
configured using a LAN communication, please perform the following
troubleshooting steps.
Verify that the IP Address is correct

1 Check the IP address and subnet mask of the Agilent 1100/1200 system by
using the control module, if available. In the System view of the control
module, select Configure > MIO for the module where the G1369A LAN card
is inserted and scroll down to the IP address of the G1369A LAN card.
NOTE
When the MIO dialog is opened on the Agilent 1100/1200 control module, the ChemStation
cannot communicate with the Agilent 1100/1200 system.
Verify that Basic Communication is possible

Use the ping command, to verify that the IP address is operational.
1 Open a command prompt window on your PC.
2 Type ping 10.1.1.102 and press Enter; where 10.1.1.102 needs to be
replaced by the appropriate IP address or by the selected host name.
The command ping will send a request for reply to the IP address, bypassing
part of the Windows TCP/IP settings. A successful ping would look like Reply
from 10.1.1.102: bytes=32 time<10ms TTL=128
If request timed out is displayed, the IP address can not be reached by the ping
command.
3 If the ping request was answered successfully by the instrument, you need
to verify that your Windows TCP/IP settings are correct for the selected
network, especially the subnet mask and gateway settings should be
chekked.
105
Troubleshooting
Identify by host name

If you identify the Agilent 1100/1200 system by host name, ensure that the
used host name and IP address are correctly set up in the DNS server or the
corresponding entry in the HOSTS file exists, see Configuring the G1369A
LAN Card Using BootP on page 100. Try using the IP address in the
Configuration Editor.
Verify that the G1369A LAN Card is correctly configured

To ensure that all parameters of the G1369A LAN card are correctly set, use
the Agilent BootP Service program from the Agilent ChemStation CD-ROM
(CD 1 - Installation) and disable any other means that configure the G1369A
LAN card of the instrument. Install the BootP Service as described in section
Installing the Agilent BootP Service Program on page 35 and configure it for
the MAC address of the LAN instrument. Power cycle the PC and the
instrument and verify that the BootP Service configures the G1369A LAN card
correctly. Thus you ensure that the parameters for the buffer handling in the
G1369A LAN card are set for analytical instruments.
DHCP Server
Check that no DHCP servers interfere with the usage of Agilent BootP Service,
as these servers also respond to bootp request. They also may send a different
IP address to the instrument each time it is started.
Frequent Buffer Overrun in Instrument Log Book

Please contact your system administrator to check if the network is not
capable of the network traffic induced due to the data acquisition of the
instruments. This can also be caused by an incorrectly configured G1369A
LAN card, see above.
106
Troubleshooting
The most common problem encountered when using I/O Config is that an
interface is not found. For Agilent 82350 A/B GPIB interface, this is due to one
of the following reasons:
Figure 44
No GPIB Interface Was Detected on the System
The interface was not installed in the PC before running I/O Config. Install
the GPIB interface card in your system and then run I/O Config again.
The interface is not firmly seated. Ensure that the board is in place and the
boards edge connectors are fully seated. Please refer to the section
Installing a GPIB Interface Board in the PC on page 39.
The GPIB interface was replaced by a different type or was installed in a
different slot. Rerun I/O Config, remove the configured card and
reconfigure the new one.
The interface has already been configured by I/O Config. To solve this
problem, you need to edit the existing configuration entry for the interface,
instead of trying to add a new interface. In the main I/O Config window,
click on the name of the interface configuration entry for the Agilent 82350
in the Configured Interfaces list box. Then click on the Edit button directly
beneath the Configured Interfaces list box to edit the configuration entry
for the Agilent 82350 GPIB interface.
107
Troubleshooting
Autoadd Button of IOCFG Installs Defaults Incompatible with the

Agilent ChemStation
IOCFG has an Autoadd button. We strongly discourage you from using the
Autoadd button because it installs default settings that are not compatible
with the Agilent ChemStation. Please configure the IO Libraries interactively
as described in Checking Out the Agilent ChemStation on page 77
108
Troubleshooting
An online core must be purchased in order to install additional

instrument modules
Most probably, a previous version of the Agilent ChemStation was installed on
the PC, but the CHEM32 directory subtree was deleted before starting setup.
CHEMSTATION.INI still contains references to the deleted Agilent
ChemStation installation in the [PCS] and [PCS,...] sections. Delete the
[PCS] sections in CHEMSTATION.INI.
ChemStation cannot be located on your system. You must first

install Agilent ChemStation before installing an instrument
Before installing an add-on instrument control module, you need to install the
Agilent ChemStation core (e.g. G2170BA).
109
Troubleshooting
Agilent ChemStation online session does not finish startup

No valid license
Before you can use the Agilent ChemStation, you need to enter the license
registration number supplied with the registration packet delivered with your
Agilent ChemStation installation disks. You need to enter a registration
number for every instrument module that you install.
If you installed the Agilent ChemStation yourself, the installation routine
prompts you to enter the license registration number of the appropriate
instrument module.
If the Agilent ChemStation core software was preinstalled by Agilent
Technologies, you need to install the instrument module software and the
license registration number.
If you do not specify the license registration number at time of installation,
you need to start the license registration utility from the Agilent ChemStations
group of Windows Program Manager. The license registration utility prompts
you for the appropriate license registration numbers.
The Agilent ChemStation does not initialize an instrument module for which
no valid license registration number has been entered.
Instrument not found

GPIB communication to the configured instrument or instrument module is
not possible. This may be caused because:
the instrument is turned off,
the instruments GPIB address does not match the address configured in
the Agilent ChemStation,
the GPIB cable is not properly connected,
the GPIB board was not correctly configured (please refer to Configuring
the Agilent 82350 GPIB Interface Drivers on page 41), or
the GPIB card is incompatible with your PC.
110
Troubleshooting
System Status shows Not Ready

The cause for being in a not-ready state is detected by the instrument.
Not-ready conditions of the Agilent liquid chromatographs are described in
the documentation accompanying the respective instrument.
Agilent ChemStation does not start

Windows needs this file to run C:\CHEM32\CORE\LAUNCHCS.EXE
The Agilent ChemStation does not start. You receive error messages that
indicate that the Agilent ChemStation initialization could not access specific
libraries. You can close all of the error message boxes.
Verify that the Agilent ChemStation main directories (default: C:\CHEM32 and
C:\CHEM32\SYS) are included in the PATH setting of the Environment Path
variable of the operating system by typing PATH at the MS-DOS prompt. If the
Agilent ChemStation system directories are listed in the PATH setting, simply
close all applications and restart the computer.
111
Troubleshooting
key in section [PCS] of CHEMSTATION.INI not valid or specified

file does not exist
File Initialization failed
The Agilent ChemStation reports an error during the initialization of its file
structure. Verify that the Agilent ChemStation data, method and sequence
path settings all point to existing directories on your PCs hard disk. You can
view these settings in the configuration editor.
Go to the [PCS] section in win.iniCHEMSTATION.INI that was specified in
the error message (for example, [PCS,1]) and check the contents of the key
entry.
If the mismatch is in the _Exe(cution)$ key, check whether the _EXEPATH$
key contains the correct path specification for the Agilent ChemStation core
modules (default: C:\CHEM32\CORE\).
If the _Meth(od)File$ key is reported to be inconsistent, verify that your
instrument method directory (for example, C:\CHEM32\1\METHOD) contains
a method directory called def_lc.m;default:method;method:defaultdef_lc.m.
If the _Seq(uence)File$ key is reported to be inconsistent, verify that your
instrument sequence directory (for example, C:\CHEM32\1\SEQUENCE)
contains a sequence file called def_lc.s.
If one or both of them do not exist, either restore a backup copy, copy the
corresponding files and directories from another Agilent ChemStation
instrument, or reinstall the software.
System Exception in dialogs.c

The Agilent ChemStation was terminated abnormally and does not restart.
This is usually caused by specific libraries of the application still being active
in the PCs memory. Close down all applications and restart Windows.
112
Troubleshooting
Configuration Editor no longer runs

If changes are made to the CHEMSTATION.INI configuration file manually, or
CHEMSTATION.INI becomes corrupted, it is possible that syntax errors or
inconsistencies are introduced into the Agilent ChemStation sections of this
file which cause the Configuration Editor to fail.
The best solution to this problem is to restore a backup copy of the
CHEMSTATION.INI file which does not have the errors. If a backup is not
available, the configuration sections can be removed manually and the
configuration restored using the Configuration Editor. To do this, edit the
CHEMSTATION.INI file using the NOTEPAD editor and remove the sections
that are preceded by [PCS,1], [PCS,2], [PCS,3], and [PCS,4], including
the section titles. Then, edit the lines for devices and instruments in the main
[PCS] section to read:
devices= instruments=
Remove all of the other device statements. At this point, you should be able to
run the Configuration Editor. Add the instruments back to the configuration
and reconfigure the instrument devices.
If the configuration information is still inconsistent and the configuration
editor does not open, we recommend that you remove the [PCS] section from
CHEMSTATION.INI and re-install the software.
113
Troubleshooting
Autostart Macro failed

During the initialization, the Agilent ChemStation automatically loads and
executes macro code from a defined set of macro files. A run time error caused
the autostart macro to fail.
A text error message is displayed in the (red) message line of the
If you have added your own customization macro code to User.Mac in the
Agilent ChemStation core directory (default path: C:\CHEM32\CORE), check
that all the macros loaded in this macro file are correctly specified.
If you cannot identify or isolate the problem, rename User.Mac to Usr.Mac and
restart the Agilent ChemStation. If the error message does not occur, you need
to debug your customization code.
If the error persists, the working copy of the Agilent ChemStations
configuration register may have been corrupted. Rename or delete the
configuration register in the appropriate instrument directory:
the configuration register for instrument 1 online is C:\CHEM32\1\
CONFIG.REG, or
the configuration register for instrument 1 off-line is C:\CHEM32\1\
CONF_OFF.REG.
Alternatively, one of the Agilent ChemStations macro files may have been
modified or corrupted. In this case, you need to reinstall the
Agilent ChemStation software.
General Protection Fault in Module...

The operating system reports a General Protection Fault (GPF) when it detects
that an application has attempted write access to a memory location that
belongs to another application or process. Potentially, a GPF is the
consequence of a system corruption. For the diagnosis of GPFs, it is
mandatory to record the exact error message and error address information in
order to identify the part of an application program that caused the failure.
Windows allows tracking of GPFs with a utility called WinDebug (WinDbg).
For more information on this utility, please refer to What is the WinDebug
Utility? on page 123.
114
Troubleshooting
Printing Problems
Printing Problems
Agilent ChemStation Print Spooler hangs after an error occurred

If the Agilent ChemStation spooler does not continue after a print error has
occurred, try initializing the Agilent ChemStation spooler by typing the
following command on the Agilent ChemStation command line:
_LoadServiceResetPrinting
The Agilent ChemStation spooler prompts you whether to cancel all pending
print jobs. If you do not want to cancel the pending jobs, press No. If the
printing error comes again, you need to save your work, close all applications
and restart Windows and the Agilent ChemStation to reinitialize the operating
environment.
Printing in Multi-Instrument configurations

When printing simultaneously from multiple Agilent ChemStation
instruments (e.g. during a sequence), resource conflicts may result in printing
errors.
If you experience printing problems that appear to be related to a temporary
shortage of available system resources as multiple programs print in parallel,
you can reduce the Agilent ChemStations frequency to hand back CPU control
to another application by typing the following command on the
Agilent ChemStation command line:
_LoadServiceChromSplYield 2000
This command specifies how often (in milliseconds) the Agilent ChemStation
Spooler lets other application use the computers CPU. The default value is
300 ms. Increasing this number increases the printing speed at the expense of
user interface response and should only be used in automated mode.
You can make this setting permanent by adding it to a macro file called
USER.MAC in the Agilent ChemStation core directory (default: C:\CHEM32\
CORE). This executes the command automatically every time the
115
Troubleshooting
Printing Problems
Agilent ChemStation is started. For further details on the customization
possibilities associated with USER.MAC, please refer to the Macro
Programming Guide which is available as online help.
Recovering from Printer hang-ups

If for some reason the communication to the printer hangs and the printer
therefore does not finish the current printout, there are two steps that have to
be done:
Depending on whether the printer is local at your PC or connected via a
network:
on a local printer do a reset on the printer panel itself, or
on a networked printer you have to clear the network communication
problem. This may involve checking cabling, or checking the printer spooler
on the network host machine, or doing a reset on the printer panel itself.
On your PC the printer driver or the Windows Print Manager has to be reset.
This is done by one of these alternatives:
if there is a printer driver dialog box visible that has just a Printing or
similar message in it together with a Cancel button, press that Cancel button,
or
if instead there is the icon of the Windows Print Manager visible, close it,
discarding the current print jobs.
If the problem persists try stopping and restarting the Windows Spooler
Service. Select Services from the Windows Control Panel, scroll down the list
and highlight the item Spooler. Choose Stop to stop the service and restart it
again selecting Start.
When the Agilent ChemStation spooler reports printer errors, a message
window displays the error messages, for example:
Printing problem 106, with page file: c:\CHEM32\1\temp\~p3d0004.tmp
These failed print files can be printed with a command typed on the command
line, for example
MFPrint "c:\CHEM32\1\temp\~p3d0004.tmp"
Do not forget to delete these temporary files after you have printed them.
116
Troubleshooting
Printing Problems
Printing Messages
Printing Problem 100
A file belonging to the current print job could not be found. Check the
consistency of your hard disks file structure.
Printing Problems 101, 102, 106, 108, 110, 210, 212, 300
Due to low system resources or insufficient disk space, a print-file could not
be accessed either in memory or on disk.
Check free system resources using the Windows Task Manager. Press
Ctrl + Alt + Del and select Task Manager. On the Performance Tab you will find
the available physical memory. If free system resources are significantly below
30 %, you should save your work, close all Windows applications and restart
Windows.

The print page could not be copied in memory. Check available memory on
your computer.

The printer driver could not be initialized.
Reset the Agilent ChemStation spooler and check the printer driver name and
version of your printer. Refer to the list of tested printers on the Agilent
ChemStation Software CD-ROM (CD 1 - Installation).
Check available system resources.

A print page could not be sent to the printer driver.
Verify that your printer is correctly configured, connected, and online.
117
Troubleshooting
Printing Problems
Printing Problems 206, 208, 302

Printer escape sequences to initialize a new page or indicate the end of a print
job could not be sent to the printer driver.
Verify that your printer is correctly configured, connected, and online.

A print job could not be removed from the print queue. Either the print job file
does not exist in the temporary directory or the Agilent ChemStation print
queue file (hpspl100.que) does not exist.
NOTE
The print queue file and Agilent ChemStation temporary files must not be deleted while the
Agilent ChemStation is running.
Printing Problem 400, 401, 402, 403

The Agilent ChemStation spooler could not be initialized properly. If this
occurs as a consequence of previous errors, save your work, terminate all your
applications and restart Windows.
Parts of the chromatogram missing on the report or strange fonts in the report
This may be caused by low system resources in Windows. Either too many
applications are running or one or more applications have not freed up all the
system resources that they allocated during their operation. Check the
percentage free of system resources in the Windows Task Manager. When free
system resources get below 30 %, you should save your work, close all
applications, and restart Windows.
118
Troubleshooting
Computer Problems
Computer Problems
Sporadic Hang-Ups
You can use DOS commands like e.g. CHKDSK to verify that there are no
inconsistencies in the file structure. If you find such inconsistencies on your
PCs hard disk, correct them. Information about maintance for your computer
can be found in the manual Configure and Maintain your Agilent
ChemStation Computer available in PDF format on the Agilent ChemStation
CD-ROM (CD 2 - User Documentation and Accessories) in the manual section.
System Crash in an Online Session

If your system crashes when trying to establish the GPIB communication to
the chromatograph, there may be hardware conflict between the GPIB board
and another device installed in your PC (for example, a specific graphics
accelerator card, an infrared port, a soundcard). Using a different IO port
and/or a different interrupt level for the GPIB card can eliminate the problem.
Refer to the GPIB card configuration information in this handbook.
Cannot create file

Check the available disk space on your system. Delete unnecessary files, for
example, left over temporary files or archive data files that you do not
currently need to a backup medium. Information about maintance for your
computer can be found in the manual Configure and Maintain your Agilent
ChemStation Computer available in PDF format on the Agilent ChemStation
CD-ROM (CD 2 - User Documentation and Accessories) in the manual section.
119
Troubleshooting
Computer Problems
Slow Hard-Disk access or Hard-Disk Activity LED is continuously

flickering
Your hard disk may be fragmented. Use a defragmentation utility to reorganize
the logical order of file clusters on your disk. If system performance is
generally low and your hard-disk appears to be accessed very frequently for
relatively long periods of time, the system is most probably low on RAM and
using virtual memory (i.e. the page file) excessively.
Reduce the number of concurrent programs.
Use Computer Management options (e.g. right-click on My computer icon and
select Manage) to access the disk management utility.
Check whether your disk cache utility is installed and optimally configured.
Agilent ChemStation Performance Degrades over Time

If your environment requires continuous routine operation of the Agilent
ChemStation over days or even weeks without restarting Windows, the system
performance may degrade over time due to memory and resource leaks in the
system. To overcome this problem, we recommend rebooting the PC on a
regular basis.
Windows Service Packs are available on the Microsoft Home page. Following
items need to be checked prior to the installation of Service Packs:
the Service Pack addresses performance issues
the Service Pack is supported with the Agilent ChemStation revision in use.
120
Troubleshooting
Agilent ChemStation does not see an LC hardware modification

(HP 1090)
After modifying the hardware configuration of the LC or an LC module, this
change may not be reflected by the Agilent ChemStation.
All of these changes require you to close the Agilent ChemStation down and
reinitialize it. Subconfiguration information of the LC hardware is only
checked at start-up time of the Agilent ChemStation.
Implementing the modification while the Agilent ChemStation is running and
simply resetting the module from the Agilent ChemStation user interface is
not sufficient.
System remains in a wait-state waiting for a module that has been

taken out of the configuration
On systems that are frequently reconfigured, i.e. devices are added or removed
from the current configuration, the system may remain in a wait-state as long
as the unused devices are still connected to the APG remote cable. The only
possible work-around is to disconnect/connect the remote cable when
deleting/adding the external module from/to the current configuration.
If a second detector is not needed for certain experiments, but for convenience
reasons is not taken out of the configuration, selecting a very short run-time
for the unneeded detector is not recommended.
For example, when acquiring data on an FLD while the DAD is configured
with a short stop-time, it may happen that the DAD lamp is switched off before
the LC analysis is finished. This produces a not ready condition so that the
next analysis does not start. Alternatively, acquiring with the DAD and
selecting a short stop time for the FLD does not produce this problem as a
lamp off status is a perfectly valid operational mode with the FLD.
121
Troubleshooting
Data File is empty (contains no signal)

Check the remote cabling of the detectors. A detector does not change into the
run-status unless the start signal is transmitted over the remote line. Refer to
the cabling diagrams in Connecting Agilent 1100/1200 modules to the
Agilent ChemStation using LAN on page 63.
Devices report Buffer Overflow in the logbook

This message usually indicates by a bad performing PC, when the data transfer
from the instrument to the PC is not capable of handling the data stream. The
bad performance can be caused by
Power save features of the PC, refer to section Advanced Power
Management Not Supported with Analytical Hardware on page 29.
Bad network performance, refer to section Frequent Buffer Overrun in
Instrument Log Book on page 106.
Bad hard disk performance, see section Slow Hard-Disk access or
Hard-Disk Activity LED is continuously flickering on page 120.
Other programs accessing the hard disk or using the computers resources,
like scheduled defragmentation, backup, or virus scanning.
122
Troubleshooting
What is the WinDebug Utility?

Microsoft developed certain diagnostic tools that provide detailed information
on the internal state of Windows when General Protection Faults (GPF) occur
on the system. WinDbg.exe is the GUI version of the debugger and supports
both user-mode and kernel-mode debugging.
The Window debugging package comes in three versions: a 32-bit version for
x86 binaries, a 64-bit version for Itanium binaries, and a 64-bit version for
AMD64 binaries. For Agilent Bundle PCs, download and install the 32-bit
version for x86 binaries.
If an application error occurs, WinDbg automatically creates a special dump
file in the file system root directory. You can enter the details about the
circumstances (scenario) under which the application error occurred and save
it with the dump file.
If General Protection Faults recur sporadically or even regularly, supply the
dump file to your application vendor to discuss the source of the application
error.
WinDebug in Windows XP
The Window debugging package comes in three versions: a 32-bit version for
x86 binaries, a 64-bit version for Itanium binaries, and a 64-bit version for
AMD64 binaries. For Agilent Bundle PCs, download and install the 32-bit
version for x86 binaries.
Install WinDbg onto your system by running the setup. The install shield
opens and the user has to accept the license agreement. After editing the user
information, click Next and select Typical as installation type. If necessary, you
may enter a installation location, click Next to start the installation. A new
group, Debugging Tools for Windows, will be present within Start > Programs.
123
Troubleshooting
NOTE
Only a system administrator can alter the postmortem settings.
NOTE
The path statements and key entries mentioned for the cmd prompt and the registry depend
on your installation directory of WinDbg.
NOTE
The dump files intentionally contain the whole process memory and may have substantial
size. Depending on the defined data values the dump file size will vary. Compress the dump
file and save it to an external media or another partition in order to maintain enough space
on your ChemStation system.
1 WinDbg needs to be the default postmortem debugger for your operating

system. To define WinDbg as default debugger, open cmd prompt by Start >
Run and type cmd in the command line. Within the cmd prompt, execute
the WinDbg program once with the parameter -I to create/change the
appropriate registry entries:
e.g.: C:\Program Files\Debugging Tools for Windows\windbg.exe -I
This command will display a success or failure message after it is used.
When WinDbg is the postmortem debugger, it will be activated whenever an
application crashes.
2 One registry entry needs to be modified to define the kind of information
placed into the dump file in case of a system crash. The modified arguments
are necessary to automatically dump all the memory information of the
failing program. The argument options can be obtained by entering the
WinDbg help.
Open Start > Run and type regedit into the command line; the registry
opens. Open the registry path
\\HKEY_LOCAL_MACHINE\Software\Microsoft\Windows NT\
CurrentVersion\AeDebug
The registry key DEBUGGER needs to be modified. By double-clicking on
the data variable, you are allowed to edit the value data. Modify the value
data from e.g.
C:\Program Files\Debugging Tools for Windows\windbg.exe" -p %ld -e %ld -g
124
Troubleshooting
to
C:\Program Files\Debugging Tools for Windows\windbg.exe" -p %ld -e %ld -Q -c
".dump -ma -u \user.dmp;q.
The data variable values may differ for certain troubleshooting task. During
the troubleshoot process, it might be necessary to modify the DEBUGGER
registry entry again.
3 In the case of a failure, a dump file named user_<date>_<time>_<pdid>.p is
placed in the files system root directory. Enter all instrument configuration
details, a description of the circumstances and steps leading to the failure
and save it with the dump file. Supply all information to your application
vendor.
125
Troubleshooting
Gathering System Information with Windows XP

Windows XP Professional include a comprehensive information reporting and
diagnostics program that collects and presents information on device driver
information, network usage, and system resources such as IRQ, DMA and IO
addresses. This utility is called System Information and can be found under
the System Tools menu in the Windows Accessories menu.
Hardware conflicts will usually also be logged in the Windows Event Viewer, if
for instance, Windows cannot start a service due to a misconfigured device.
For Windows XP Professional Error Reporting is implemented and enabled by
default.
To access the settings for the reporting feature:
1 Click Start.
2 Right-click My Computer, then click Properties.
3 Click on the Advanced tab.
4 Click Error Reporting.
For more information, refer to Windows XP Professional help.
126

8 Upgrade History - Previous Revision

A/B ChemStations
Introduction
128
129
Agilent ChemStation A.02.0x
129
129
130
131
131
132
132
132
133
134
Agilent ChemStation B.01.0x
134
134
With Agilent ChemStation B.01.0x release the Agilent ChemStation made a progressive move towards new feature sets and structure changes. Due to this the
new Agilent ChemStation is starting with a new major revision change: B.01.0x.
Within this Chapter a summary of the introduced new functionality for the revision cycle A03.x till B.02.x is documented.
127

Introduction
Introduction
With ChemStation B.0x.0x release, the Agilent ChemStation made a

progressive move towards new feature sets and structure changes. The
revision number beginning with B indicates the major revision change.
Within this chapter, a summary of the new functionality introduced for the
revision cycle A.01.x to B.02.x is documented. For all implemented defect
fixes, refer to the HISTORY directory on the Agilent ChemStation software
family CD-ROM (CD 2 - User Documentation and Accessories).
128


Data Analysis is now part of the method settings and stored in a binary
register file.
A.01.0x includes an older version of the Integrator module. The values
accepted for the threshold settings have been redefined. To make the
integration threshold settings equivalent on both platforms, the threshold
values need to be increased by 5.
The conventions followed by G1304/5A (HPLC2D ChemStation), revision
A.01.0x, regarding the origin handling in calibration curve calculations are
different from the conventions followed in the later revisions. Revision
A.01.0x offers three different modes for origin handling (ignore, include,
force origin). The definition of Force Origin has been changed (see below). A
new origin handling method Connect Origin has been added, which does
exactly what Force Origin did in A.01.0x.

Methods now include a dilution factor, in addition to the multiplier that was
already part of revision A.02.xx methods.
Column informationis restored when the new software is started for the first
time. You have to select the currently installed column from the column
information screen to make this information available on the reports. Column
information is only restored for columns that have been used and identified
for the current system. Column information from methods transferred from
other systems is not restored.
129


The Agilent ChemStation supports the enhanced integrator since revision
A.04.01. Using the enhanced integrator is optional. Methods imported from a
previous version of the Agilent ChemStation can continue to use the original
integration algorithm.
The user interface allows the conversion of a method imported from a
previous version of the Agilent ChemStation. Once a method has been
converted to use the new enhanced integration algorithm, it cannot be
converted back.
To evaluate the enhanced integration algorithm of the Agilent ChemStation,
we recommend that you create a backup copy of your original method.
The parameter sets of the original Agilent ChemStation integrator and the
enhanced integrator are different. For example, parameters such as the initial
threshold are values to the power of 2 of a detector specific constant.
The enhanced integrator uses the true physical dimensions for integration
parameter settings like slope sensitivity (Response/Time) or height reject
(Response).
The results calculated by the two integrators may vary on real chromatograms
due to the differences in the determination of baselines, shoulders, and
tangent skimmed peaks.
The Internal Storage Format of Quantification Data changed. Revisions
prior to A.04.01 used single precision floating point representation for the
internal storage of quantification data; the internal precision of the
quantification results used 7 digits.
In revision A.04.01, the internal storage format has been changed to double
precision floating point representation, and the internal precision of
quantification calculations and results is now 15 digits.
The following changes have been implemented in the sequencing user interface
and internal structure of the Agilent ChemStation.
The sequence table has been enhanced with an Append Line button.
The hard-coded link between sample information and specific vials has
been removed to allow for easier cut/copy/paste operations on sequence
lines.
The partial sequence screen has been equipped with a print button.
130

Better integration of Sequence Summary into the sequence user interface.
Sequence Summary Setup is now accessible through a new menu called
Sequence Output.
Sequence recalibration table has been eliminated from the method.
A new sample type has been implemented to allow for quality control
samples. Control samples can be used to verify the systems suitability to
run a defined set of analyses prior to running the real samples. If the
defined system suitability criteria are not met, the sequence can be
programmed to stop before running the real samples.

The default integration algorithm used by revision A.05.01 and higher of the
Agilent ChemStation is the Enhanced Integrator. In previous revisions, the
default integration algorithm used was the Standard Integrator. Data analysis
methods could be converted to the Enhanced Integrator.
The user interface of the Peak Purity function available in the
Agilent ChemStations for LC and LC/MS has been simplified, but required
modifications to the peak purity parameters stored in the data analysis
method. If you convert to the Enhanced Peak Purity function, the previous
spectra settings are saved in a text file called SPCOPS.OLD in the method
directory. Once you have converted a method to use the Enhanced Peak Purity
function, you cannot revert back to the original method. Make a backup copy
of your original method before converting.

The methods used for operational qualification and performance verification
(enhanced OQ/PV) of the Agilent 1100 system were enhanced. The standard
methods can be used by selecting the Standard Tests from the Options menu in
the Verification view of the Agilent ChemStation. Additional software
verification tests were also added that are used by the Agilent ChemStation
A.06 OQ/PV service.
131

The advanced baseline option was added to the enhanced integrator introduced
in revision A.04.01. The tangent skim option was enhanced with respect to
A.05.01. The default setting for both options did not change.
Calibration points weights 1/Y and 1/Y2 were added to the calibration table.
The report now also allows to generate HTM files for direct posting on a web
server.

The sequence filldown utility allows the user to change sequence table column
settings for specified vial ranges. Sequence table columns can be selected and
values entered for method, sample type, updating response factors and time.
Sample name and file name can be given a prefix and auto incremental
number.

The ability to control the new Capillary LC system that is designed for
increased sensitivity and for analyzing limited sample volumes was added.
The software now supports the Agilent 1100 Series well-plate autosampler,
which is available in a standard and thermostatted version.
With revision A.08.0x, you can upgrade the Agilent Chemstation product to
the Agilent Chemstation Plus Security Pack supporting the FDA requirements
of CFR 21 Part 11.

The ability to control the new Agilent 1100 Series Fraction Collector and the
Preparative Scale 1100 Autosampler, as well as the Agilent 1100 Series
Purification System for HPLC and LC/MS.
The 35900D A/D card is no longer supported (card based on the older ISA bus
standard).
132


The ability to control the new Agilent 1100 Series Fraction Collectors:
G1364B
Preparative Scale
G1364C
Analytical Scale
G1364D
Micro Fraction Collector
The Agilent Chemstation supports the new 40 funnel tray for high volume
collection for high flow rates and the new tray for Eppendorf tubes (80.5 ml,
1.5 ml and 2.0 ml).
The A.10.0x ChemStation software supports the Agilent 1100 Series
Purification System for HPLC and LC/MS, version A.02.01.
The new XML based interface has been implemented to link the
Agilent Chemstation to LIMS and knowledge management systems.
133


Agilent ChemStation revision B.01.xx is supporting the use of long file names,
a higher resolution for the screen and improvements and enhancements for
the integrator. In addition following new hardware modules are supported:
G4240A Agilent 1100 Chip Cube
G1315C Agilent 1100 Diode Array Detector
G1365C Agilent 1100 Multi Wavelength Array Detector (80 Hz, 8 Signals)
USB-GPIB interface support has been added for GPIB communication based LC
and CE systems (HP 1090, HP 1046, HP1049, CE, CE/MS).
For CE ChemStation users enhancements have been added in B.01.03
ChemStation:
More flexibility defining vial usage set points within the sequence setup
Enhanced productivity for CE ChemStation customers due to instrument
set points modification directly in the sequence table for each sequence
line.

Agilent ChemStation revision B.02.xx is supported with the M.01.01 GPIB
drivers and SICL libraries for the 82350A/B card as well for the 82357
USB-GPIB interface. The software introduces an improved User Interface
design along with a tree and table based navigation, providing fast and flexible
data handling and the possibility to configure flexible storage locations for
data, methods and sequences. A new packaging concept guarntees consistency
fore sequence and single sample data and makes use fo the new data review
and reprocessing capabilites in the Data Analysis Navigation Table.
134

9 Supplementary Information
136
137
Calling the Agilent Technologies LSCA Customer Contact Center 138
135
Revision numbers are created according to the scheme outlined below:

P.RR.xxY
P-
Denotes the identifier of the series of a product. This letter is identical to

suffix character in the product number.
Example:
The B in the product number G2170BA defines that revision code starts with a
series identifier B. It is current Agilent Technologies policy not to change the
series identifier unless the software changes in purpose or function. The A in
the product number stands for the American English version.
136
RR-
Represents the major revision number. Changes in this code typically include
significant software enhancements that may require complete revalidation of
the system for customers operating in regulated environments. These
revisions may also include defect fixes and documentation changes.
xx-
Represents the minor revision number. These revisions correct software

defects and are generally isolated in nature. They may contain minor
enhanchments or new features, but the functionality has not changed. A minor
revision change usually does not require revalidation of the system for
customers operating in regulated environments. These revisions only include
defect fixes that do not affect the accuracy of the handbooks.
Y-
Represents the localisation code. This character states the localised version of
Software. For example, the B.01.01C is representing the Chinese version of the
Agilent ChemStation. Note, this additional code is only present for localized
versions, for standard American English version no Y suffix will be present.
Agilent Technologies offers different options to help you obtain the

professional assistance you need to achieve maximum productivity with your
Agilent Technologies analytical software. This service is available nearly
worldwide through our Customer Contact Centers.
The Agilent Technologies network of Customer Contact Centers provides
access to support professionals who help you resolve operational difficulties
and offer assistance and advice on running Agilent Technologies analytical
software. Traditionally, this support is given over the telephone but may also
be extended to remote support via modem when you authorize us to do so.
Software support is available for your Agilent Technologies software solution.
First year software support is available and may be extended by two years at
very competitive rates. This support provides entitlement to telephone
assistance, software revision upgrades when they are released, and regular
delivery of Software Status Bulletins containing important information on
known problems and available work-around solutions for your Agilent
Technologies analytical software. For more information on how to register for
these services, please contact your local Analytical Support Representative.
Your local Analytical Support Representative will also provide information on
available consulting, customization, development, and training services on
Agilent Technologies analytical software products.
Agilent Technologies support and update services are subject to your local
countrys prices, terms, and conditions ruling at the time you place your order.
137
When you call the Agilent Technologies Customer Contact Center, please be at
your computer and have the product documentation at hand.
We recommend that the following information be readily available:
the registration packet label with the product number, revision code, and
license registration number of your analytical software
the exact wording of any error messages that the system produced
a list of the instrument modules including firmware revisions that are
connected to the PC. You may use the Serial number information from the
Agilent ChemStation (Method/Run control>Instrument (in Full Menu view only)
> Serial numbers and Columns) to generate this list.
a printout of the Dumpfile (see What is the WinDebug Utility? on
page 123)
a full printout from Windows System Information
a description of the scenario that leads to the failure.
138
Index
Index
1
1046
1049
70
70
A
address
default GPIB
71
advanced power management
29
Agilent 1100 Series
CAN cabling
62
connecting
62
controller area network (CAN)
90
GPIB cabling
62
Agilent 1100 system access
91
Agilent 35900 dual channel interface
connecting
70
Agilent 82350
39, 41, 38
Agilent 82357A USB - GPIB Interface 46
Agilent 82357A USB-GPIB Interface
38
Agilent ChemStore
14
APM
29
area calculation mode
129
Autoadd Button
108
autostart macro failed
114
B
bootp server
BootP
buffer handling
buffer overflow
buffer overrun
bus address
C
cable
94
35
102
122
106
46
GPIB
40, 67, 66
remote
63
cabling
62
CAN
Agilent 1100 Series
62, 90
cannot create file
119
cannot find file
111
changing
area calculation mode 131, 132, 133
checkdisk utility
119
checksum
78
ChemStation.ini
22
ChemStation
connecting Agilent 1100 Series
62
connecting HP 1090 LC
66
connecting miscellaneous instruments
70
uninstalling
22
CHKDSK
119
COM board
76, 76
commands reference
12
computer problems
119
computer settings
16
config.reg (configuration register)
114
configuration editor
87
configuration register
114
configuration
editor
90
instrument devices
93
paths
88
connecting
printer cable
28
connector
remote
71
controller area network
Agilent 1100 Series
90
Agilent1100 Series
CTL board
62
76
D
DAD
HP 1040
DAP board
def_lc.m
def_lc.s
default sequence
default
GPIB addresses
method
sequence
detector
electrochemical
filter photometric
HP 1040 diode-array
HP 1046 fluorescence
HP 1049 electrochemical
device configuration dialog
diode-array detector
HP 1040
dual channel interface
dual channel
70
76
88
88, 112
88
69, 70, 71
88
88, 112
70
93
70
70
70
91
70
70
70
E
electrochemical detector
HP 1049
enhanced
integrator
70
130
F
file initialization failed
FILES setting
112
117
139
Index
filter photometric detector

firmware revisions
fluorescence detector
HP 1046
fluorescence
93
72, 76
70
70
G
GPF
123
GPIB addresses
Agilent1100
37, 65
default
69, 70, 71
HP 1090
69, 70
GPIB cables
66
GPIB interface
26
GPIB
Agilent 1100 addresses
37, 65
cable
40, 62, 67, 66
connector
67, 66
HP 1090 addresses
69, 70
H
hardware address
94, 96
hardware
installing
26
requirements
28
host name
102
how-to information
12
HP 1040 diode-array detector
connecting
70
HP 1046 fluorescence detector
connecting
70
HP 1049 electrochemical detector
connecting
70
HP 1090 liquid chromatograph
connecting
66
GPIB cable
66
HP 82335
38
hpced02.exe
87
I
I/O config utility
87
installation qualification
78
installation verification
78
INSTQ
79
instrument log book
buffer overrun
106
instrument not found
110
instrument types in configuration editor 90
integrator
enhanced
130
interface
dual channel
70
IOCFG, autoadd button
108
iocfg.exe
87
IQ
78
105
20
110
110
46
M
MAC address
102, 94, 96
macro programming guide
12
MCO/MEM board
76, 76
method
default
88
mobility report
130, 131, 132, 133
multi-instrument configurations
printing
115
O
online core
operation qualification
109
83
P
PCS section
power fail
print spooler
printing messages
printing problem
printing problems
product structure
112
86, 105
115
117
117
115
13
qualifying
operation
quantification data
internal storage format
83
130
R
reference files
78
reference information
11
remote cable
67
remote
cable
63
connector
71
report
mobility
130, 131, 132, 133
resource conflicts
27
revision history
10
revision numbers
136
N
non-HP computers
140
111
111
30
L
LAN communication
troubleshooting
license registration number
license registration utility
license, no valid
logical unit
not ready state

not ready system status
NT Workstation
27
Index
S
sequence
default
88, 112
service pack 3
30
serviceinfo macro
138
SICL interface name,
46
signal distribution module
76
software certificate and registration packet
20
software start-up problems
109
software
requirements
28
specified file does not exist
112
system controller
46
system exception
112
system status
not ready
111
Windows 2000 Professional

Windows 95
30
30
X
XML support
21
T
task-orientated information
TCP/IP
test
verification
11
34
83
U
uninstalling the ChemStation
USB-GPIB
user.mac
22
38
114
V
verification
test
VISA interface name
83
46
W
win.ini
WinDbg.exe
21
123
141
www.agilent.com
In This Book
Use this handbook when you install the Agilent
ChemStation for the first time or when you
make changes to an installed system. This
handbook describes how to install the initial
software, how to add further instrument
modules, how to configure your analytical
system, and how to verify that the installation
and configuration are complete and
operational.
This handbook lists the PC hardware (see
Operating System Requirements" on page 39)
and software (see Operating System
Requirements" on page 39) requirements that
need to be met in order to install and operate
the Agilent ChemStation successfully and
explains PC configuration details that you will
find useful when upgrading your computer,
optimizing your system, or when trying to solve
an installationrelated problem. You may skip
these chapters if your Agilent ChemStation was
fully installed by Agilent Technologies.
Agilent Technologies 2004, 2005-2007
Printed in Germany
02/2007
*G2170-90024*
*G2170-90024*
G2170-90024

2007 Installing Your Chemstation

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Agilent ChemStation for

Installing Your ChemStation

Manual Part Number

Restricted Rights Legend

Installing ChemStation for LC Systems

Installing Agilent ChemStation

Installing Communication Components

Installing the Analytical Instruments

Installing ChemStation for LC Systems

Checking Out the Agilent ChemStation

Configuring the Analytical System

Upgrade History - Previous Revision A/B ChemStations

Installing ChemStation for LC Systems

Installing Agilent ChemStation

Agilent ChemStation PC Hardware Details

Installing Communication Components

Installing LAN Communication

Installing the Analytical Instruments

Connecting an Agilent 1100/1200 Series LC to the Agilent ChemStation

Checking Out the Agilent ChemStation

Agilent ChemStation Installation Verification

Configuring the Analytical System

Using the Agilent ChemStation Configuration Editor

Installing ChemStation for LC Systems

Troubleshooting LAN Communication

Upgrade History - Previous Revision A/B ChemStations

Agilent ChemStation Revision Code Convention

Installing ChemStation for LC Systems

Installing ChemStation for LC Systems

1 Installing Agilent ChemStation

Contents of the two Agilent ChemStation CD-ROMs

Agilent ChemStation Modules

Installation Verification Utility for Installation Qualification

Agilent ChemStation Software Status Bulletin (SSB)

Agilent ChemStation Revision History

Agilent ChemStation Product Documentation

What Is Reference Information and Where Is It?

What Is Task-Oriented Information and Where Is It?

Where Can I Find the Description of Agilent ChemStation

I Upgraded from a Previous Version - How Do I Know What Has

Additional Data Evaluation Modules

Installing the Agilent ChemStation

Activating the XML based interface

Uninstalling the Agilent ChemStation

This chapter provides a description of the installation process. It gives you an

Installing Agilent ChemStation

To ensure the successful installation of the Agilent ChemStation and the

Installing ChemStation for LC Systems

Installing Agilent ChemStation

Contents of the two Agilent ChemStation CD-ROMs

Agilent ChemStation Modules

Installation Verification Utility for Installation Qualification

Installing ChemStation for LC Systems

Installing Agilent ChemStation

Agilent ChemStation Software Status Bulletin (SSB)

Agilent ChemStation Revision History

Installing ChemStation for LC Systems

Installing Agilent ChemStation

Agilent ChemStation Product Documentation

What Is Reference Information and Where Is It?

Installing ChemStation for LC Systems

Installing Agilent ChemStation

What Is Task-Oriented Information and Where Is It?

Where Can I Find the Description of Agilent ChemStation