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Volume 1; Issue - 4; Year 2015; Page: 298 306
Abstract
Xanthophyll are the typical yellow pigment leaves that play major role in the metabolism of plants.
The different Arbuscular mycorrhizal (AM) fungi were screened for their mutualistics effect with marigold
(Tagets erecta L.) var. Salem lemon yellow. The present investigation was carried out in Pot culture house,
Department of Microbiology, Annamalai University. Five AM fungal species were used Glomus
fasciculatum, Glomus mossae, Gigaspora margarita, Glomus versiforme and Acaulospora laevis. All the
inoculated seedlings showed significant results over control after 30, 60 and 90 days after transplanting.
Tagets erecta L. (Var. Salem lemon yellow) seedlings raised in the presence of Arbuscular mycorrhizal fungi
showed highest root colonization percentage, spore number, acid phosphatase activity, alkaline phosphatase
activity, relative mycorrhizal dependency and xanthophyll content compared to uninoculated control plants.
It was observed that Glomus fasciculatum the best AM fungal Symbiont for marigold plants compared to the
others.
Key words: AM fungi, Glomus fasciulatum, Xanthophylls and Marigold.
1. Introduction
Ramlakshmi/ Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(4): 298 306
299
percent
root
colonization
in
Ramlakshmi/ Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(4): 298 306
300
g
A474 D
petal meal =
kg
W 236
Where,
A474 = Absorbance at 474 nm
W = weight of the sample (petal meal) in g
50 100
3
236 = translation specific absorptivity for 1
gm/litre
D = Final dilution =
Ramlakshmi/ Indo Asian Journal of Multidisciplinary Research (IAJMR), 1(4): 298 306
Control
T2
100% NPK
T3
G. fasciculatum
T4
Bacillus megaterium
T5
T6
T7
T8
T9
T10
T11
T12
T13
301
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Table -1: Screening on AM fungal isolates for colonization spore population and phosphatase
activity in Marigold
S.
No.
Root colonization
(%)
AM fungal isolate
Acid phosphatase
Alkaline phosphatase
activity
activity
(g/ 24 hrs-1 10g-1 of root) (g/ 24 hrs-1 10g-1 of root)
30
DAT
60
DAT
90
DAT
30
DAT
60
DAT
90
DAT
30
DAT
60
DAT
90
DAT
30
DAT
60
DAT
90
DAT
Glomus mosseae
42.23
58.43
65.20
146.20
168.90
179.23
28.56
29.60
31.53
27.30
28.59
29.55
Glomus fasciculatum
54.20
65.26
79.23
155.17
172.42
184.43
29.51
30.73
31.73
28.70
30.03
31.36
Glomus versiforme
28.27
40.23
50.20
129.73
160.03
167.45
25.43
27.20
28.43
24.17
24.86
25.20
Acaulospora laevis
39.50
52.30
59.16
141.46
165.86
173.83
27.30
28.40
30.23
26.06
27.10
27.46
Gigaspora margarita
32.23
45.36
55.16
134.43
160.93
169.68
26.46
28.01
29.21
24.56
25.63
25.65
SE
1.59
1.13
1.03
1.19
0.76
1.25
0.36
0.23
0.41
0.49
0.45
0.69
CD (p = 0.05)
3.19
2.25
2.06
2.35
1.53
2.49
0.76
0.45
0.83
0.96
0.93
1.39
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Table 2: Screening of AM fungal isolates for relative mycorrhizal dependency and mycorrhizal inoculation effect of
S.No.
1
2
3
4
5
6
7
A
B
C
D
E
F
Treatments
Sterilized soil
Unsterilized soil
Unsterilized soil + G. mosseae
Unsterilized soil + G. fasciculatum
Unsterilized soil + G. versiforme
Unsterilized soil + A. laevis
Unsterilized soil + Gi. Margarita
SE
CD (p = 0.05)
Relative mycorrhizal dependency
Mycorrhizal inoculation effect of G. mosseae
Mycorrhizal inoculation effect of G. fasciculatum
Mycorrhizal inoculation effect of G. versiforme
Mycorrhizal inoculation effect of A. laevis
Mycorrhizal inoculation effect ofGi. Margarita
Marigold
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Table 3: Effect of AM fungi (G. fasciculatum) and phosphobacteria (Bacillus megaterium var phosphaticum) with graded levels of
inorganic phosphorus on the xanthophyll content of Marigold at harvest stage
Treatments
T1
T2
T3
T4
T5
T6
T7
T8
T9
T10
T11
T12
T13
Control
100% NPK alone
G. fasciculatum alone
Bacillus megaterium alone
100% NPK + G. fasciculatum
100% NPK + Bacillus megaterium
100% NPK + G. fasciculatum + Bacillus megaterium
75% P and 100% N&K + G. fasciculatum
75% P and 100% N&K + Bacillus megaterium
75% P and 100% N&K + G. fasciculatum + Bacillus megaterium
50% P and 100% N&K + G. fasciculatum
50% P and 100% N&K + Bacillus megaterium
50% P and 100% N&K + G. fasciculatum + Bacillus megaterium
SE
CD (p = 0.05)
Xanthophyll content
gkg-1
kgha-1
33.43
12.73
33.00
14.60
35.10
15.50
35.00
15.43
37.95
17.80
37.60
17.60
41.01
20.80
39.95
19.56
38.05
18.40
43.15
22.66
36.99
15.80
36.46
15.60
39.03
19.60
1.05
0.90
2.13
1.83
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