Documente Academic
Documente Profesional
Documente Cultură
Hamilton Campus
Session 2013-14
Trimester 1
Instructions to Candidates:
1. Answer ALL multiple choice questions from section A.
Answer ONE question from section B.
Answer FOUR questions from section C.
2. Ensure you enter your student number on any
Graph paper used
Multi choice answer sheet
and attach it to the answer booklet.
3. Calculators must not be used to store text and/or formulae nor be
capable of communication. Invigilators may require calculators to be
reset.
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Section A
(Multiple Choice Questions)
Answer ALL Questions using the multi choice answer sheet provided
1.
2.
What is a chromophore?
(a) A group of atoms in a compound responsible for the
emission of electromagnetic radiation
(b) A group of atoms in a coloured compound
(c) A group of atoms in a compound responsible for the
absorption of electromagnetic radiation
(d) A coloured compound
(e) A fluorescent label attached to a non-coloured compound
3.
4.
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5.
6.
with
UV
detection
is
7.
8.
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9.
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16. Which of the following is commonly used as the mobile phase in GCMS?
(a)
(b)
(c)
(d)
(e)
H2O
He
CH3CN
Air
Acetylene
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20. In a mixture of the five proteins listed below, which should elute second
in size-exclusion (gel-filtration) chromatography?
(a) Cytochrome C, Mr = 13,000
(b) RNA polymerase, Mr = 450,000
(c) Serum albumin, Mr = 68,500
(d) Immunoglobulin G, Mr = 145,000
(e) Transferrin, Mr = 80,000
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Section B
Answer ONE Question
1.
2.
(10)
(10)
(10)
(10)
(i)
(ii)
accuracy
precision
3.
(10)
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(10)
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Section C
0B
1B
1.
With the aid of the infrared and nuclear magnetic resonance correlation
charts provided, fully interpret the spectra Worksheet Q1 IR, Worksheet
Q1 NMR and Worksheet Q1 MS and hence deduce which of the
following structures A, B or C correlates to the spectra given.
A.
B.
C.
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Worksheet Q1 IR
3H
Worksheet Q1 NMR
Integration is
numeric
3H
2H
2H
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Worksheet Q1 MS
Q1 Worksheet MS
2.
(2)
b) Which type of cell would you use for this experiment, and why?
(1)
c) Using the results below and the graph paper provided plot a
calibration graph for the phosphate standards.
(4)
Tube Number
1
2
3
4
5
Phosphate (mg dm-3)
1
2
3
4
5
Absorbance values 0.15 0.32 0.50 0.59 0.72
d) How can you tell if your calibration graph conforms to the
Beer-Lambert law?
(2)
(1)
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3.
4.
(4)
(6)
a)
(2)
(i)
(ii)
(iii)
(2)
(2)
b) Explain what is meant by gradient elution in hplc and comment on
the advantages it brings to the process.
5.
(4)
(2)
(2)
(3)
(3)
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6.
Molecular Weight
Isoelectric Point
Protein B
68,000
5.8
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