Documente Academic
Documente Profesional
Documente Cultură
Aquatic Botany
journal homepage: www.elsevier.com/locate/aquabot
University of Nice-Sophia Antipolis, EA 4228 ECOMERS, Lab. Environnement Marin littoral, Parc Valrose, 06108 Nice Cedex 2, France
Canakkale Onsekiz Mart University, Faculty of Fisheries, Terzioglu Kampusu, 17100 Canakkale, Turkey
c
Dokuz Eylul University, Institute of Marine Science and Technology, Haydar Aliyev Bulv No. 32 Inciralti 35340 Izmir, Turkey
d
Stazione Zoologica Anton Dohrn, 80121 Napoli, Italy
e
Istanbul University, Institute of Marine Science and Management, Muskule sok., Vefa, 34470 Istanbul, Turkey
b
A R T I C L E I N F O
A B S T R A C T
Article history:
Received 8 November 2007
Received in revised form 8 April 2008
Accepted 24 April 2008
Available online 3 May 2008
The seagrass Posidonia oceanica is a stenohaline species endemic to the Mediterranean Sea, where it
normally lives at a salinity of between 36.5 and 39.5 ppt. Surveys carried out at the North-eastern
distribution limits revealed large beds in the Dardanelles Strait and isolated beds in the Marmara Sea,
where the salinity ranges between 21.5 and 28 ppt. Microsatellite analysis of these low-salinity tolerant
P. oceanica beds, show different signs of genetic isolation: excess of heterozygosity and a presence of xed
alleles. These particularities are rarely found in the whole distributional range of the species. Moreover,
all the populations considered in the analysis have a very low genetic diversity in comparison with most
of the meadows sampled throughout the Mediterranean Sea. Taking into consideration the genetic data,
rhizome expansion rate and the actual extent of the isolated beds in the Marmara Sea and knowing the
reproductive rate and dissemination characteristics of P. oceanica, we hypothesize that the isolated
population of the Marmara Sea has been established since the Middle Holocene, before the catastrophic
intrusion of brackish water into the Marmara Sea and the strong and persistent ow coming from the
Black Sea.
2008 Elsevier B.V. All rights reserved.
Keywords:
Seagrass
Posidonia oceanica
Marmara Sea
Salinity tolerance
Genetic isolation
1. Introduction
Posidonia oceanica (L.) Delile (Magnoliophyta) is the most
abundant seagrass in the Mediterranean Sea and its distribution is
fairly well recorded (Green and Short, 2003). However, its
distribution limit is still unclear particularly at the narrow
Dardanelle Strait where the Aegean Sea meets the Marmara Sea
and previous records appear to be incorrect. The record of P.
oceanica reported in the Marmara Sea in The World Atlas of
Seagrasses (Green and Short, 2003), was in fact observed at UrlaIskele, close to Izmir in the Aegean Sea (Short, pers. com.).
This endemic Mediterranean species is known to be stenohaline, living in a salinity range between 36.5 ppt (in the Alboran Sea:
Ramirez et al., 2005) and 39.5 ppt (in the Aeagean Sea: Besiktepe,
2003). The species can occasionally tolerate very high salinity up to
48 ppt in semi-enclosed environments, such as the Stagnone
coastal lagoon at Marsala (Di Maida et al., in press.), but up to now
it has not been recorded in low salinity environments. The
presence of the species in the Marmara Sea is exceptional because
of its brackish water. Moreover, the regular and vigorous intrusion
19
Fig. 1. Distribution of Posidonia oceanica in the Dardanelles Strait and in the Marmara Sea.
Table 1
Summary of genetic data for the ve populations analyzed
N
G/N
Fis
He
H0
Wt
Ass
Erdek
Latitude 40 28
Longitude 27 40
30
0.30
21
0.704
0.246
0.426
***
Dardanos
Latitude 40 02
Longitude 26 04
30
24
0.80
24
0.073
0.263
0.288
NS
Kucukkuyu
Latitude 39 31
Longitude 26 32
30
11
0.37
24
0.189
0.213
0.261
NS
1 Dar
3 Dar
Lesbos (Eftalou)
Latitude 39 22
Longitude 26 12
18
13
0.72
22
0.306
0.234
0.314
**
1 Erd
Dikili
Latitude 39 09
Longitude 26 47
30
14
0.47
25
0.017
0.231
0.234
NS
2 Ntu
2 Ntu
N: number of samples; G: number of genotypes; L: number of polymorphic loci; A: number of alleles; He: expected heterozygosity; H0: observed heterozygosity, Wt: Wilcoxon
test for heterozygosity excess signicant at 99% (***), 95% (**) or not signicant (NS); M: number of rst generation migrants and population of origin; Ass: number of
genotypes assigned to any other population.
20
21
22
Boudouresque, C.F., Thommeret, J., Thommeret, Y., 1980. Sur la decouverte dun
bioconcretionnement fossile intercale dans lherbier a` Posidonia oceanica de la
Baie de Calvi (Corse). Journees Etudes Systematique et Biogeographie Mediterraneennes. Cagliari, CIESM, 139142.
Cagatay, M.N., Gorur, N., Algan, O., Eastoe, C., Tchapalyga, A., Ongan, D., Kuhn, T.,
Kuscu, I., 2000. Late glacial-Holocene palaeoceanography of the Sea of Marmara: timing of connections with the Mediterranean and the Black Seas. Mar.
Geol. 167, 191206.
Cirik, S., Guner, H., 1979. Analyse bibliographique des travaux sur les phanerogames
et les algues marines benthiques des cotes de Turquie (18431978). Rev. Biol.
Ecol. Mediterraneennes 6, 93100.
Cornuet, J.M., Luikart, G., 1996. Description and power analysis of two tests for
detecting recent population bottlenecks from allele frequency data. Genetics
144, 20012014.
Coyer, J.A., Diekmann, O.E., Serrao, E.A., Procaccini, G., Milchakova, N., Pearson, G.A.,
Stam, W.T., Olsen, J.L., 2004. Population genetics of Zostera noltii (dwarf eelgrass) throughout its biogeographic range. Mar. Ecol. Progr. Ser. 281, 5162.
Di Maida, G., Calvo, S., Pirrotta, M., Tomasello, A., Borra, M., Procaccini, G., in press.
Seagrass meadows at the extreme of environmental tolerance: the case of P.
oceanica in a semi-enclosed coastal lagoon. Mar. Ecol. Evol. Perspect.
Fernandez-Torquemada, Y., Sanchez-Lizaso, J.L., 2005. Effects of salinity on leaf
growth and survival of the Mediterranean seagrass Posidonia oceanica (L.)
Delile. J. Exp. Mar. Biol. Ecol. 320, 5763.
Green, E.P., Short, F.T., 2003. World Atlas of Seagrasses. Univerity of California Press,
UNEP-WCMC, Berkeley, USA.
Kendrick, G.A., Marba`, N., Duarte, C.M., 2005. Modelling formation of complex
topography by the seagrass Posidonia oceanica. Estuar. Coast. Shelf Sci. 65,
717725.
zsoy, E., Oguz, T., U
nluata, U
., 1991. Observations of the Mediterranean
Latif, M.A., O
inow into the Black Sea. Deep Sea Res. 38, 711723.
Major, C., Ryan, W., Lericolais, G., Hajdas, I., 2002. Constraints on Black Sea outow
to the Sea of Marmara during the last glacial-interglacial transition. Mar. Geol.
190, 1934.
Marba`, N., Duarte, C.M., 1998. Rhizome elongation and seagrass clonal growth. Mar.
Ecol. Progr. Ser. 174, 269280.
Mateo, M.A., Romero, J., Perez, M., Littler, M.M., Littler, D.S., 1997. Dynamics of
millenary organic deposits resulting from the growth of the Mediterranean
seagrass Posidonia oceanica. Estuar. Coast. Shelf Sci. 44, 103110.
Meinesz, A., Lefe`vre, J.R., 1984. Regeneration dun herbier de Posidonia oceanica
quarante annees apre`s sa destruction par une bombe dans la rade de Villefranche
(Alpes-Maritimes, France). In: Boudouresque, C.F., Jeudy de Grissac, A., Olivier, J.
(Eds.),Proceedings of the 1st International Works Posidonia Oceanica Beds, vol. 1.
GIS Posidonia Pub., Marseille, pp. 3944.
Migliaccio, M., De Martino, F., Silvestre, F., Procaccini, G., 2005. Meadow-scale
genetic structure in Posidonia oceanica L. (Delile). Mar. Ecol. Progr. Ser. 304,
5565.
Molenaar, H., Meinesz, A., 1991. Vegetative reproduction in Posidonia oceanica II.
Effect of depth changes on transplanted orthotropic shoots. PSZNI Mar. Ecol. 13,
175185.
Myers, P.G., Wielki, C., Goldstein, S.B., Rohling, E.J., 2003. Hydraulic calculations of
postglacial connections between the Mediterranean and the Black Sea. Mar.
Geol. 201, 253267.