44 Tumulak, Jamaica Marie M.

2B-PH
ASSIGNMENT IN MICROBIO LAB
DIFFERENT TYPES OF MICROSCOPE
1. SIMPLE MICROSCOPE
In this type of microscope, the convex
lens of short focal length to see magnified
image of a small object. It also consists of
a biconvex lens which is moved up and
down by the use of an adjustment screw
to bring the object in sharp focus. The
object is placed on the platform and light
is focused with the help of a concave
mirror. This microscope can also be called
a dissecting microscope.

2. LIGHT/COMPOUND MICROSCOPE
This kind of microscope which is widely
used in
high school and college
laboratories consists of two set of convex
lenses. It uses multiple lenses for the
enlargement of a sample image or
specimen. It also acquired higher levels of
magnification than a stereo or low power
microscope. The objective lenses are
considered as one of the most significant
parts of a compound microscope, as they
are the lenses closest to the specimen.
3. TRANSMISSION ELECTRON
MICROSCOPE

This type of electron microscope

functions under the same basic
principles as an optical microscope. It
utilizes various energetic electrons to
provide morphologic, compositional
and crystallographic information on
samples.
Transmission
electron
microscopes can be considered as the
most powerful microscopes at a
maximum potential magnification of 1
nanometer. For the benefits of the
educational, scientifical and industrial
applications, this microscope was
constructed to produce high-resolution
and two-dimensional images.

4. SCANNING ELECTRON
MICROSCOPE
This kind of electron microscope is used
to study the detailed surfaces of the
cell and organisms. In this microscope,
the formation of the image is due to
the electrons reflected back from the
object. The image formed by this
microscope has a remarkable three
dimensional appearance. The said
microscope requires a stable power
supply, vacuum and cooling system
and vibration-free space. It is a must
that scanning electron microscopes are
housed in an area where it is isolated
from ambient magnetic and electric fields.

5. PHASE CONTRAST MICROSCOPE

This type of microscope is widely used to
study the behavior of living cells,
observe the nuclear and cytoplasmic
changes taking place during mitosis and
the various effects of different chemicals
inside the living cells. It also enables the
viewing of live microorganisms. By using
the phase-contrast microscope, an image
of strong contrast of the object is
obtained. One of the major advantages
of phase contrast microscopy is that the
examination of living cells in their natural
state can be without previously being
killed, fixed, and stained.

6. INTERFERENCE MICROSCOPE
Interference microscope utilizes light
interference phenomena for the creation
of two superimposed images of an object
that will pave the way for the possible
observation
of
transparent
objects
without using a staining technique. This
type of microscope is also used for
quantitative studies of macromolecules
of the cell components like lipids, nucleic
acids and proteins.

PARTS OF A COMPOUND
MICROSCOPE