Volume 5, Issue 5, May 2015, Pages 399403

Document heading

GC-MS analysis and antibacterial activity of Cuscuta

reflexaagainst bacterial pathogens

Rafiqul Islam,
Md. Shahedur Rahman, ,
Shaikh Mizanur Rahman
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doi:10.1016/S2222-1808(14)60804-5
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Abstract
Objective
To find out the antibacterial efficacy of Cuscuta reflexa (C. reflexa) and
examine the chemical composition of the essential oil from C. reflexa by
gas chromatography and mass spectrometry (GC-MS).
Methods
Antibacterial potency of C. reflexa plant extracts has been tested
against Bacillus subtilisIFO 3026, Sarcina lutea IFO 3232, Xanthomonas
campestris IAM 1671, Escherichia coliIFO 3007, Klebsiella
pneumoniae ATTC 10031, Proteus vulgaris MTCC 321 andPseudomonas

denitrificans KACC 32026 by disc diffusion assay. The chemical

compositions of the essential oil were analyzed by GC-MS.
Results
GC-MS analysis identified sixteen compounds from essential oil of C.
reflexa, of which piperitone (15.19%), caryophyllene (12.55%), -elemene
(10.35%), elemol (6.20%), -selinene (4.49%), -humulene (3.98%) and
thujone (5.18%) were the major compounds. -Bergamotene and juniper
camphor were identified as the minor compounds. Dichloromethane and
petroleum ether extracts of C. reflexa showed a great potential of
antibacterial activity against all tested bacteria. Minimum inhibitory
concentrationvalues of various extracts ranged from 16 to 512 g/mL.
Conclusions
These extracts showed good antibacterial activity and could lead to new
antibacterial drug designing.

Keywords

Cuscuta reflexa;
Antibacterial activity;
Disc diffusion assay;
GC-MS
Available online 20 Mar 2015
Foundation Project: Supported by Jessore University of Science and
Technology, Jessore- 7408, Bangladesh (grant number
GEBT/JSTU/LET/2013-119).
Corresponding author: Md. Shahedur Rahman, Department of Genetic Engineering
and Biotechnology, Jessore University of Science and Technology, Jessore- 7408,
Bangladesh

Copyright 2015 Asian Pacific Tropical Medicine Press. Published by Elsevier (Singapore)
Pte Ltd. All rights reserved.

Asian Pacific Journal of Tropical Biomedicine

Volume 2, Issue 3, Supplement, 2012, Pages S1664S1666

Document heading

Minimum Inhibitory Concentration Analysis of Nerium

oleanderagainst Bacterial Pathogens

M. Abu Hena Mostofa Jamala,

Shahedur Rahmanb, , ,
Azizul Islama,
Rezaul Karima,
Samsul Alama,
Ziaur Rahmana
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doi:10.1016/S2221-1691(12)60473-0
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Abstract
Objective

In this present study, it is tried to find out the antimicrobial effect and
Minimum Inhibitory Concentration (MIC) of Nerium
oleander against Bacillus subtilis (IFO 3026), Sarcina lutea (IFO
3232), Escherichia coli (IFO 3007) and Klebsiella Pneumoniae (ATTC
10031).
Methods
Powered leaves were prepared and used for extraction with various
solvents, viz, the petroleum ether, and chloroform extract of the oleander.
All the solvent extracts were evaporated to dryness. Using the disc
diffusion method, the bacterial growth were inhibited,
Results
Among the solvent extracts tested, petroleum ether extract inhibited the
growth of all the tested bacteria having various degrees of inhibition zones.
Highest inhibitory activity was observed against E. coli (1.9 cm) and
minimum inhibitory concentration was observed 2g/ml also against E. coli.
Both results were observed in case of petroleum ether extract. Petroleum
ether extract also showed inhibitory zones of 1.8 cm, 1.4 cm and 1.5cm
against B. subtilis, S. lutea and K. pneumoniae. On the other hand
chloroform extract was observed to have inhibition zones of 1.2 cm, 1.6 cm,
1.8 cm and 1.5 cm against B. subtilis, S. lutea, E. coli and K.
pneumoniae respectively.
Conclusions
The study demonstrated that the petroleum ether extract of N. oleander is
potentially good source of antibacterial agents. Further evaluation is
necessary to identify the specific bioactive compounds, their mode of action
and their nontoxic nature in vivo condition.

Keywords

Nerium oleander;
MIC;
Escherichia coli (IFO 3007);
Petroleum ether extract
Available online 28 December 2012
Corresponding author: Md. Shahedur Rahman, Department of Genetic Engineering
and Biotechnology, Jessore Science and Technology University, Jessore-7408,
Bangladesh, Tel: +8801553116736
Copyright 2012 Asian Pacific Tropical Biomedical Magazine. Published by Elsevier
(Singapore) Pte Ltd. All rights reserved.