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Part 1
Laboratory Orientation
A Course Information
You have already been introduced to this course via
the course outline at the start of the lab manual.
It is important that you understand the structure and
organization of this course in order that you make
the most of the learning opportunities on offer.
You will sit in the same seat as you are sitting
today, on every day that you attend a laboratory
session.
During laboratory sessions there will be instances
when half of the group complete a task first and the
other half second. When this occurs you will be
divided according to your seating location.
Those seated nearest the main entrance are sitting
in the East seats and the remaining students are in
the West seats.
Class representatives serve an important role in this
paper. Class reps meet with staff three times during
the semester and relay comments from the class to
the staff in order to improve the course.
Task A.1
Aim
This session will comprise an introduction to
the course and laboratory protocols.
Objectives
B.
C.
D.
E.
F.
G.
and
the
Task complete
19
Lab 1 Introduction
Question A.1
Question B.1
Question A.2
Question B.2
Question A.3
Make a note of your seat position. Are you at
an East seat or a West seat? What are the
names of the two people sitting next to you?
A.
Question A.4
C Laboratory Safety
It is the responsibility of all of us to ensure a safe
working environment for ourselves and also for
those around us. In order to achieve this the
following rules must be observed:
1.
Shoes must be worn in the laboratory at all
times. Lab coats must be worn unless you are
instructed otherwise.
B HUBS administration
The following person should be your first point
of contact for most matters relating to this
course:
Title: Course Administrator
Name: Mike McArthur
Phone: 479 7737
Email: mike.mcarthur@stonebow.otago.ac.nz
The names of other teaching staff are available
on Blackboard.
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2.
Personal belongings must be stored away
from public thoroughfares. Do not leave your coat
or bag where someone may trip over it. Do not
leave valuables in the corridor.
3.
Do not put anything in your mouth while in
the laboratory.
4.
All accidents, must be reported at once to
the demonstrator or lab supervisor. Do not attempt
to clean up spills of body fluids. Notify a
demonstrator at once.
Lab 1 Introduction
Question C.2
Should you clean up your own spills in the
lab?
No. You need to inform a demonstrator
immediately.
5.
Pay attention to hazardous chemicals
information. You will be made aware of all
known hazards in your working environment
before you begin work. It is your responsibility
to listen and take any steps required to ensure
your own safety. If you dont understand, ask!
6.
You are responsible for ensuring the
safety of those about you, as well as yourself.
7.
All specimens of human origin must be
considered as capable of transmitting infectious
disease and treated accordingly (see safety
manual or ask the laboratory supervisor).
Question C.3
Discuss with your neighbour: Do you know
the names of any such infectious diseases that
are carried via body fluids?
Fig 1.1 Location of fire exits
Examples are hepatitis C and B and HIV.
Question C.5
8.
All cuts and abrasions on hands must be
covered with an occlusive (not perforated)
dressing.
Question C.4
Why would a normal plaster not suffice?
Task C.1
9.
Any skin puncture must be reported to
the laboratory supervisor immediately.
10.
Familiarise yourself with the location
and use of safety equipment such as safety kits,
eye wash stations, fire extinguishers and fire
exits (see fig. 1.1).
IN THE EVENT OF A FIRE ALARM - when
no fire is evident in this lab, students on the
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Lab 1 Introduction
11.
Exercise care when disposing of material
after laboratories. Do not endanger the health
of technical staff by disposing of hazardous
material in the wrong place. You will be
informed where potentially dangerous material
should go.
12.
Report any unsafe practices or situations
to the laboratory.
13.
Do not remove any material from the
laboratory that you did not bring in yourself.
14.
Wash your hands in the hand basin
before leaving the laboratory.
Question C.6
What should you do if you accidentally splash
something in your eyes?
A.
Course documents
Lectures (includes audio lecture resources)
Laboratories (includes Lab correct or model
answers)
Self-Directed Learning (includes specific
resources, SDL correct or model answers
and links to the assessment area)
Glossary
Integrated Case Study
Discussion board
Contacts
Staff Information
Class Representatives
Assessment
SDL Assessments
Terms Test 1
Terms Test 2
Final Examination
Check Your Grade
Question D.1
What is the integrated case study and what is its
purpose?
A video scenario that is related to questions and
discussions throughout the course. This allows
integration of the anatomy, physiology and
microbiology information learnt during the
semester.
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Question D.2
How often do you intend to visit the Blackboard
site, given the information that you know is
available on Blackboard.
A personal choice, but we recommend regular
visits.
Lab 1 Introduction
Question E.1
Question E.2
No.
F Learning styles
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Lab 1 Introduction
Check-in structure
You will be asked to answer 5 questions on the
computer before starting each lab session. These
questions refer to the content of the laboratory.
For this to run smoothly, please ensure that you
arrive five minutes early for every lab session.
You will not be given your check-in results until
the end of the lab. This is to ensure that your lab
performance is not influenced by your check-in.
Check-out structure
At the end of each laboratory session, we will ask
you to answer five randomly assigned questions on
the labs content. In the 5th question you will
apply your knowledge to the integrated case.
If you have shown the required level of knowledge
and understanding, you are awarded 1%.
If you have not passed, a demonstrator will discuss
your check-out answers with you. After this
discussion you will be given an opportunity to
revise and then attempt check-out a second time.
If you need further help, we will ask you to attend
a tutorial after which there will be a third
opportunity to complete the check-out.
Question G.1
What percentage of your final grade does each
check-in and check-out assessment count for?
Check-in; 0%, check-out; 1%.
Question G.2
After the lab what should you do to ensure that
you get the most from each laboratory session?
You will need to look up the model answers for
the session on Blackboard.
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Lab 1 Introduction
Part II
Anatomical Terminology
and Skeletal Anatomy
Aims
The first steps in understanding human movement
are to become familiar with the structures involved
and with their relationships within the body.
In this practical session you will be introduced to
anatomical terminology including the terms used
to describe movements. You will also be
introduced to the structure and form of human
bones and their function within the human
skeleton.
Objectives
By the end of Part II you should be able to:
A.
B.
C.
D.
E.
25
Lab 1 Introduction
Task A.1
Feet ..............................................
Palms...........................................
Head............................................
Task complete
Task A.3
Assume the standard anatomical position again
and demonstrate anterior and posterior to your
neighbour.
Task complete
Question A.3
Which structures lie anterior to the heel of the
foot?
Question A.1
The toes.
Is the standing skeleton demonstrating the
standard anatomical position? If not which
body parts are out of place?
Medial - closer to the median plane (see fig 1.4).
The upper limb may hang such that the palms
do not face forwards.
Directional Terms
Anatomical terms of direction and position
describe the position of one structure relative to
another. They are always related to the standard
anatomical position.
Task A.4
Assume the standard anatomical position again
and demonstrate medial and lateral to your
neighbour.
Task complete
Question A.2
Which structure lies superior, the nose or the
chin?
The nose.
26
Lab 1 Introduction
Task A.5
Assume the standard anatomical position again
and demonstrate proximal and distal to your
neighbour.
Question A.7
Complete the sentence:
In the human body the skin can be described as
.................................. in relation to bone or
muscle.
Superficial.
Task complete
Question A.6
Now move out of the standard anatomical
position by raising your upper limb (arm) above
your head and bending at the elbow so that your
hand is behind your head. Is it still correct to say
that the wrist is distal to the elbow and why?
Yes
Directional terms are always relative to the
anatomical position.
Question B.1
Demonstrate to your neighbour how you could
cut/section your own body into two pieces along
the median sagittal plane. Fill in the spaces:
You would be left with an equally sized
................... and ......................half of your body
Right and left..
Task A.6
Assume the standard anatomical position again
and demonstrate deep and superficial to your
neighbour.
Task complete
27
Lab 1 Introduction
Task B.1
Locate the sagittal and coronal sutures (joint
lines) on the skull of a skeleton. Draw a superior
view of the skull, labeling these two suture lines
and the anterior and posterior aspects of the
skull.
back
Question B.4
Demonstrate to your neighbour how you could
cut/section your own body into two pieces along
the transverse plane. If you took a section at the
level of the umbilicus (belly button) would you
be left with similarly shaped portions of your
body?
No, your head and shoulders would be in one
portion and your legs and forearms in the other.
Question B.5
Stand in the standard anatomical position. The
palms of your hands lie in which plane?
Coronal.
Task complete
C. Identification of Types of
Bones
For the following tasks you will examine one of
the articulated skeletons (on stands) and the
disarticulated bones (loose bones on each table) to
become familiar with the range of sizes and
shapes. Bones are grouped into broad categories
of long, short, flat and irregular shapes. Use
the laminated sheet to help you if you need it.
Long bones - These bones, not surprisingly, are
longer than they are wide. They consist of a shaft
and two expanded ends, with articular surfaces
forming joints at the extremities.
28
Lab 1 Introduction
i) Axial Skeleton
Task C.1
Identify an example of a long bone, a flat bone
and an irregular bone and then label them on fig
1.6 in your lab manual.
Task complete
Question C.1
To which category would the following bones
belong?
Thigh bone.
Task D.1
Identify and examine the cranium and then label
it on fig 1.6 in your lab manual.
Task complete
Finger bones.
Shoulder blade.
Task D.2
29
Lab 1 Introduction
Task D.6
Task complete
Question D.2
Note the size, shape and position of the humerus
and the femur. Name as many characteristics as
you can that:
a) they have in common.
Task D.4
b) are different.
Task complete
Task D.5
Identify and examine the radius and the ulna and
then label them on fig 1.6 in your lab manual.
Task complete
Task D.7
Question D.1
How many bones are there in the arm and what
is/ are the name(s)
One, the humerus.
30
Lab 1 Introduction
Fig 1.6 The human skeleton (plate 10, adapted from Kapit & Elson, The Anatomy Coloring Book, Harper
Collins, 1977).
31
Lab 1 Introduction
Question D.3
What difference can you see between the leg
bones (i.e. the tibia and fibula) and the bones in
the forearm (i.e. radius and ulna)?
Question D.4
What important function of the lower limb
might explain these differences and those
already noted between the femur and humerus?
Locomotion and weight bearing.
Question D.5
How many bones are there in the leg and what
is/ are the name(s)?
Fig 1.7 Anatomical terms for movement.
Two, tibia and fibula.
Task E.1
Stand in the anatomical position and then
demonstrate to your partner flexion and
extension at the following joints.
elbow
wrist
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hip
knee
Task complete
Lab 1 Introduction
Question E.1
Task E.2
Stand in the anatomical position, abduct then
adduct at the following joints:
shoulder
wrist
hip
Task complete
Task E.3
Stand in the standard anatomical position and
circumduct your shoulder and hip.
Task complete
Question E.2
Pronate and supinate your forearm and the
forearm of a skeleton. What happens to the
radius (relative to the ulna) in full pronation?
The radius comes to lie diagonally across the
ulna.
33
Lab 1 Introduction
Question E.3
A right-handed person unscrewing a jar lid
needs to forcefully perform which movement?
Pronation.
If you started with Part III, you should revise in
preparation for check-out.
Dorsiflexion is when the toes are lifted upwards,
as in standing on the heel (note that this can also
be referred to as flexion).
Plantarflexion occurs when the toes are pushed
downwards, as in standing on tip-toes (note that
this can also be referred to as extension, so there is
potential for confusion).
Inversion - Turning the sole of the foot inwards.
Eversion - Turning the foot outwards.
Task E.5
Perform each of these four movements,
demonstrating them to you partner.
Task complete
Opposition is the movement of the thumb that
brings it across the palm to touch the tip of any
finger. This is a special movement as it allows for
the manipulation of objects.
Task E.6
Demonstrate opposition to your neighbour.
Task complete
Question E.4
Do most other mammals have opposable
thumbs?
No. Primates do have opposable thumbs
allowing them to manipulate objects and this is
a specialized skill.
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Lab 1 Introduction
Part III
Introduction to Data
Collection in Physiology
Aim
Objectives
A.
B.
C.
Introduction
Physiology is the study of the function of the body,
that is, how the bodys cells, tissues, organs and
systems work.
To determine how the body works, physiologists
use the scientific method: a hypothesis is proposed
and is then tested by experimentation. The
experimental results either support or refute the
hypothesis. This is the process by which the body
of scientific knowledge is advanced.
The physiological data that you will collect in your
experiments are often small analog signals. It is
necessary to use specialized recording equipment
to observe such data. The data acquisition system
you will use is PowerLab and the software
program is LabTutor. In this part of the lab, you
will learn the principles of data acquisition and
how to use of this system.
Experiment
Introduction to Data Collection
Background Theory
Most biological signals are analog signals e.g.
sound waves. The data acquisition system,
Powerlab, converts analog signals into a digital
format that can then be displayed on the computer.
In fig 1.10 a mechanical signal, the pulse sensed in
the finger, is first converted into an electrical
signal by a transducer. PowerLab digitizes this
electrical signal and then it is displayed on the
computer.
35
Lab 1 Introduction
Experiment Aim
To record the electromyogram (EMG) as a means
to learn how to use the LabTutor system for the
collection of physiological data.
Question 2
What does the abbreviation EMG mean?
E electro
M myo
G gram
Procedure
Work in pairs. Today, due to time constraints, we
will record from one subject only.
10 minutes
Go to the Introduction to LabTutor
experiment. Read pages 1 - 4.
Question 1
Circle the Bio Amp input on figure 1.11. This is
where the recorded signal feeds directly into the
PowerLab for interpretation and then displayed
in real time on the computer.
Answer
Question 3
What generates the electrical signals recorded in
an EMG?
An EMG is a surface recording of net electrical
activity that arises as action potentials are
conducted along the skeletal muscle cells
membranes.
Question 4
Name another electrical recording that is
routinely used in clinical diagnoses.
ECG and EEG.
10 minutes
Continue with the Introduction to
LabTutor experiment (page 5). The setup for recording an EMG is shown in fig 1.12.
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15 minutes
Continue with the Introduction to
LabTutor experiment (pages 7 14).
Record your EMG and learn how to optimize the
data you collect.
Lab 1 Introduction
Results
Print the LabTutor report (page 14) and paste it into the space below.
Insert EMG recording and graph here:
37
Lab 1 Introduction
Question 5
What is the relationship between the strength of
muscle contraction and the amplitude of the
EMG?
An increase in strength of muscle contraction
correlated with an increase in the average
amplitude of the EMG (best shown from
measurements of positive integral amplitude).
Question 6
Do your observations support the hypothesis
that electrical activation of skeletal muscle leads
to its contraction?
Support.
Question 7
What further experimental evidence would you
need to prove that electrical activity initiates
skeletal muscle contraction?
Disrupt the electrical activity and see if muscle
still contract. ?how disrupt the electrical
activity: nerve damage, ECF,T dcr.
Task
Insert the LabTutor icons for the following:
Start icon
Autoscale icon
Marker icon
Delete icon
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