1What is the average nucleotide length recognized by restriction enzymes?

A. 1-3
B. 4-6
C. 5-6
D. 1-7
2What are the ends of each DNA segment cut by restriction endonucleases called?
A. Sticky Ends
B. Heated Ends
C. Coarse Ends
D. Non-Sticky Ends
3Which kind of RNA did Cohen and Boyer attempt to find the presence of?
A. tRNA
B. miRNA
C. rRNA
D. siRNA
4What was the name of the plasmid vector Cohen and Boyer attempted to construct?
A. pUC19
B. pAG24
C. pSC101
D. pSC104
5How are DNA of different lengths separated?
A.
B.
C.
D.

Gel electrophoresis
PCR
Western Blot
Southern Blot

6Which two of the following can RFLPs be used for?

A.
B.
C.
D.

Forensic Science
Genetic Markers
Mathematics Proofing
Physics

7Which two of the following are traditional ways vaccines are made?
A.
B.
C.
D.

Killed Strain
Mutated Strain
Attenuated Strain
Defective Strain

8How are recombinant vaccines created?

A.
B.
C.
D.

Cloning a gene from a pathogen into a host organism

Using reverse transcriptase to incorporate viral DNA into a host
Killing a virus and injecting it into a host
None of the above

9What is the name of the bacteria that can transfer a Ti plasmid to broadleaf plants?
A.
B.
C.
D.

Agrobacterium tumefaciens
Agrobacterium rhizogenes
Agrobacterium radiobacter
Agrobacterium albertimagni

10What kind of DNA in the Ti plasmid causes tumor formation?

A.
B.
C.
D.

T DNA
cDNA
Autosomal DNA
X Chromosome DNA

11Which of the following two purposes is Southern Blot used for?

A.
B.
C.
D.

Verify the absence or presence of a nucleotide sequence

Identify the size of the restriction fragment
Count the number of nucleotides present in the restriction fragment
Determine the exact length of the fragment

12To what is the fragile gel transferred to?

A.
B.
C.
D.

Nylon filter
Agarose filter
Polysynthetic filter
Steroid Filter

13Why cant eukaryotic DNA be directly cloned into prokaryotic cells?

A.
B.
C.
D.

Introns
Exons
Mutations
DNA Polymerase

14What enzyme is used to help clone eukaryotic DNA into prokaryotic cells?
A. DNA Polymerase I
B. DNA Polymerase III

C. Reverse Transcriptase
D. Lactase
15What enzyme is used to join DNA fragments with plasmids?
A. DNA Ligase
B. Topiomerase
C. Helicase
D. DNA Polymerase
16The kind of plasmid used for cloning has how many restriction sites?
A.
B.
C.
D.

One
Two
Three
None

17What does Taq Polymerase use as a starting point to form complementary DNA?
A. Primers
B. Deoxyribonucleotides
C. RNA polymerase
D. DNA Polymerase I
18How many molecules of DNA can be produced after 21 cycles?
A. More than 1 million
B. More than 2 million
C. More than 3 million
D. More than 1 thousand
19A microarray consists of what?
A. oligonucleotide probes
B. nucleotide probes
C. DNA probes
D. None of the above
20The presence of genes for antibiotic resistance can be determined by
A. Hybridizing cDNA
B. Destroying cDNA
C. Transforming cDNA
D. None of the above

1. B
2. A
3. C
4. C
5. A
6. A, B
7. A, C
8. A
9. A
10. A
11. A, B
12. A
13. A
14. C
15. A
16. A
17. A
18. A
19. A
20. A