Technical Sheet

AccuGENX-ID Identification Report

Guide for BacSeq and FunITS
Interpretation
Sample C1234567 - 20131212001 has a closest match of Staphylococcus pasteuri and links directly to it in the Neighbor Joining
Tree. Both the sample and its closest match have identical sequences in the first 500 base-pair region of the 16S gene. The result is
Staphylococcus pasteuri, with a Species level of confidence.

Section Descriptions
1. Your company name

223 223
LakeLake
DriveDrive
Newark,
DE 19702
Newark,
DE 19702
Phone
+1.302.292.8888
Phone
+1.302.292.8888
+1.302.292.8468
Fax Fax
+1.302.292.8468
www.criver.comRaccugenix
www.criver.comRaccugenix

2. Ship To address from your Identification Request Form

3. Unique sample code (C#) assigned by Charles River
4. Your sample ID, as provided on the Identification Request Form
5. Your 6-digit Account #, followed by a 4-digit alpha-numeric code in parenthesis

Address:
Address:

Accugenix
C#: C#:3
Accugenix

10231023
YoleYole
Drive,
Drive,
Newark,
Newark,
DE 19702,
DE 19702,
United
United
States
States

Due Due
Date:Date: 7

Accugenix
Database
Search
Result
- BacSeq8
Accugenix
Database
Search
Result
- BacSeq

Confidence
Level:
Confidence
Level:
Species
Species
10

Staphylococcus
pasteuri9
Staphylococcus
pasteuri

8. Test code for this sample as submitted by the customer

Sequence
Sequence
Alignment
Alignment

9. Final identification result

10. Confidence level of the identification (Species, Species*, Genus, Family, Class,
Order or No Match (for fungi only: the organism was sequenced successfully, but
no taxonomic classification could be confidently made based on our current ITS2
fungal library))

12

!535!C1560128-20131125115
!535!C1560128-20131125115
Alignment:
Alignment:
!535!C1234567-20131212001
!535!C1234567-20131212001
%!535!Staphylococcus
0.090.09
%!535!Staphylococcus
pasteuri
pasteuri
%!535!Staphylococcus
1.211.21
%!535!Staphylococcus
warneri
warneri
%!535!Staphylococcus
2.342.34
%!535!Staphylococcus
devriesei
devriesei
%!535!Staphylococcus
2.432.43
%!535!Staphylococcus
caprae
caprae
%!535!Staphylococcus
2.522.52
%!535!Staphylococcus
epidermidis
epidermidis
%!535!Staphylococcus
2.622.62
%!535!Staphylococcus
hominis
hominis
hominis
hominis
%!535!Staphylococcus
2.622.62
%!535!Staphylococcus
capitis
capitis
capitis
capitis
<ettensis
<ettensis
%!535!Staphylococcus
2.802.80
%!535!Staphylococcus
hominis
hominis
novobiosepticus
novobiosepticus
lugdunensis
lugdunensis
%!535!Staphylococcus
2.802.80
%!535!Staphylococcus
<ettensis
<ettensis
2.802.80
%!535!Staphylococcus
hominis
hominis
novobiosepticus
novobiosepticus
%!535!Staphylococcus
lugdunensis
lugdunensis

11

Neighbor
Neighbor
Joining
Joining
TreeTree
N Join:
1.240
1.240
% %
13 N Join:

14

C1234567-20131212001
C1234567-20131212001
Staphylococcus
Staphylococcus
pasteuri
pasteuri
Staphylococcus
Staphylococcus
warneri
warneri
Staphylococcus
Staphylococcus
epidermidis
epidermidis
Staphylococcus
caprae
caprae
Staphylococcus
Staphylococcus
Staphylococcus
capitis
capitis
capitis
capitis
Staphylococcus
devriesei
devriesei
Staphylococcus
Staphylococcus
Staphylococcus
<ettensis
<ettensis
Staphylococcus
hominis
hominis
hominis
hominis
Staphylococcus
Staphylococcus
Staphylococcus
hominis
hominis
novobiosepticus
novobiosepticus
Staphylococcus
Staphylococcus
lugdunensis
lugdunensis

--------------------------------------------------------------Not
--------------------------------------------------------------Not
intended
intended
for infor
vitro
in vitro
diagnostic
diagnostic
use-------------------------------------------------------------use--------------------------------------------------------------

PagePage
1 of 1 of 1

14. Neighbor Joining Tree visually represents the genetic differences between your
isolate and its 10 closest matches

2013-12-12
2013-12-12

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

7. The due date of your Identification Report

13. The % value is the length of the line next to it; similar to a legend on a map, it
provides a horizontal distance scale for the Neighbor Joining Tree. The % value is
somewhat arbitrary but falls between the % differences of the 1st and 10th closest
matches.

ID Request
ID Request
FormForm
#: 10000
#: 10000
6

C1234567-20131212001
C1234567-20131212001

Customer
Sample
Customer
Sample
ID: ID:
Example
Example
4

Identification:
Identification:

12. The result of the alignment represents the percent genetic difference, or # of
nucleotide differences, between the unknown sample sequence and the library
entry sequence.

SOP-GEN-017
SOP-GEN-017

------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Account: 5
Account:
110440
110440
( ACC1
( ACC1
)
)
Valued
Customer
Customer:
Customer:
Customer
1 Valued

6. Each ID Request Form received with samples has a unique number.

11. Your sample and the top 10 closest matches in the Accugenix sequence library

Accugenix
Accugenix
AccuGENX-ID
AccuGENX-ID
Report
Report

15

Prepared
Prepared
By Kerry
By Kerry
Falgowski
Falgowski
on 2013-12-30
on 2013-12-30
11:56:36
11:56:36
Reviewed
By Emily
Huang
on 2013-12-30
12:08:47
Reviewed
By Emily
Huang
on 2013-12-30
12:08:47
QA Approved
QA Approved
By Don
By Ryan
Don Ryan
Aquino
Aquino
on 2013-12-30
on 2013-12-30
12:13:18
12:13:18

Rev.Rev.
27Jan2014
EH EH
27Jan2014

This document
This document
has been
hassigned
been signed
electronically
electronically
by the by
individual(s)
the individual(s)
shown shown
above,above,
in compliance
in compliance
with FDA
withregulation
FDA regulation
CFR 21
CFR
Part21
11.
Part 11.

15. Electronic signatures in compliance with FDA regulation 21 CFR Part 11

askcharlesriver@crl.com
www.criver.com
2014, Charles River Laboratories International, Inc.