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CHEM 23A
MF 7:00-10:00 AM
I.
Introduction
Saliva in humans is a mouth fluid possessing several functions
involved in oral health and homeostasis, with an active protective role
in maintaining oral healthiness. Saliva helps bolus formation by
moistening food, protects the oral mucosa against mechanical damage
and plays a role in the preliminary digestion of food through the
presence of - amylase and other enzymes. [1]
In humans, oral fluid originates mainly from three pairs of major
salivary glands (parotid, sublingual and submandibular) and from a
large number of minor salivary glands. Parotid glands are entirely
serous glands since their secretion lacks mucin, whereas
submandibular and sublingual glands are mixed sero-mucous. Minor
salivary glands are mainly Von Ebner glands (entirely serous organs
situated in the connective tissue below the circumvallatae papillae)
and Blandin-Nhm mucous glands [2].
Hence, this experiment aims to test if there is a presence of
amylase, mucins, calcium and inorganic phosphate in the saliva which
can be seen as colored precipitates.
II.
Methodology
III.
Results
A. Collection of Saliva
Amount of saliva collected: 3 mL
Time after required amount was collected: 11 mins.
Salivary flow rate: 3mL / 11 mins. = 0.273 mL/min
IV.
Discussion
The result for the salivary flow rate was 0.273 mL/min. In this
case, the salivary flow rate is low. This means that the saliva is not
functioning to its full potential and the oral health is at risk. The
saliva may not flow and protect surfaces effectively and the general
acidity in the mouth will be favoring demineralization and/or
increasing colonization of aciduric bacteria. (Dawes, 1996)
Furthermore, individuals with low salivary flow rate may experience
dry lip. (Hansel, et. al, 2000). The extended use of saliva flow rate
test would probably lead to the earlier detection of hypo salivation
and thereby in many cases the early detection of underlying
diseases.
V.
VI.
References
[2] Dawes, C., Ong, BY. (1973). Circadian rhythms in the flow rate
and proportional
contribution of parotid to whole saliva volume in man. Arch
Biol 18:1145-53.
[3] Dawes, C. (1996). Factors influencing salivary flow rate and
composition. In: Saliva and oral health. WM Edgar and DM
OMullane editors. London: British Dental Association, pp.
27-41.
[4] Fox, PC. (1996). Differentiation of dry mouth etiology. Adv Dent
Res 10:13-6
[5] Hoffer, R., Saul, E. (2000). Oral status of 81 subjects with eating
disorders. Eur J Oral Sci 07:157-63
[6] Pai, M., et al. (2013). Viscosity of whole saliva. Acta Odontol
Scand 56:210-4
[7] Fulop, B., et al. (2014). Amylase-exploring digestion. Retrieved
at www.seplessons.org/mode
[8] Noriega, T. (2014). Amylase in saliva. Retrieved at
www.seplessons.org/mode/2443