Documente Academic
Documente Profesional
Documente Cultură
I. Multiple Choice. Choose the absolute best answer for each item. There may be
more or less than one answer for each item.
1. Which of the following statements is (are) true?
a. bacterial ribosomes consist of 40S and 60S subunits
b. prokaryotic DNA is normally complexed with histones
c. prokaryotic DNA normally exists as a closed circle
d. circular DNA is supercoiled
2. Why is RNA more vulnerable to alkaline hydrolysis than DNA?
a. the 2 OH in its ribose group can react with the phosphodiester bonds of
the
chain,
cleaving it
b. the lack of a complementary strand allows access for nucleases (enzymes
that cleave nucleic acids)
c. A and B
d. none of the above
For the next 7 numbers, refer to these 3 choices:
a. primary
quaternary
b. secondary
c. tertiary
d.
3. What is the level of structure exhibited by the major and minor grooves of DNA?
4. What is the level of structure exhibited by mRNA in its functional form?
5. What is the highest level of structure exhibited by tRNA in its functional form?
6. It is the level of structure described as the three-dimensional arrangement of all
atoms in a biomolecule.
7. It is the level of structure in proteins described as the order of amino acid
residues in the polypeptide chain.
8. It is the level of structure in proteins described as the interaction between
subunits in proteins that consist of more than one polypeptide chain.
9. It is the level of structure which pertains to the hydrogen-bonded arrangement of
the polypeptide backbone.
For the next 2 numbers, use the following choices. The antisense strand of a double
stranded DNA starting from the phosphate end reads ATTTGCAC.
a. 5 AUUUGCAC 3
d. 3 UAAACGUG 5
b. 5 GUGCAAAU 3
e. 3 ATTTGCAC 5
c. 5 UAAACGTG 3
f. 3 TAAACGTG 5
12. A friend tells you that only four different kinds of bases are found in RNA. What
would you say in reply?
you would tell him to go back to high school because he is wrong
you would tell him that there are certain cases where more than 4 bases are
present
C. you would tell him that he is correct
D. you dont tell him anything because you have no friends
A.
B.
13. One way of determining the denaturation of DNA (you can think of this as the
double stranded DNA separating to its 2 complementary strands) is by observing a
hyperchromic shift. In laymans terms, this is the phenomenon where the denatured
single stranded DNA is absorbing much more light than when it was still double
stranded. You have 2 pure DNA samples with the same length but differing in the
base pairs. When you heated both samples to 70 degrees Celsius and checked the
amount of light absorbed by each sample, you find that sample A had a
hyperchromic shift while sample B did not. Assuming that you did not degrade the
DNA, what can be a reason for this phenomenon?
a.
b.
c.
d.
Sample
Sample
Sample
Sample
A
B
A
B
contains
contains
contains
contains
a
a
a
a
spider web
sodium potassium pump
ribosome
nucleus
c. phosphodiester bond
d.
20. What is the bond responsible for forming beta sheets in the secondary structure
of proteins?
21. As you are aware, there are 2 forms of beta sheets, parallel and antiparallel.
Which of them is more stable?
a.
b.
c.
d.
antiparallel
parallel
both have the same stability
insufficient information
22. What are some of the possible bonds in a protein tertiary structure?
a.
b.
c.
d.
e.
boiling it at 100 C
placing it inside a freezer (-20 C)
exposing it to ultraviolet light
adding minute amounts of urea to it
adenine
cytosine
guanine
thymine
uracil
ionic bonds
covalent bonds
hydrogen bonds
hydrophobic interactions / Van der Waal interactions
Questions were edited & taken from Campbell and Farrell Biochemistry 7 th ed., www.biologycorner.com, and Prof.
Espanos MBB 10 slides.
V2 (10/12/2015) | Prepared by the UP Molecular Biology and Biotechnology Society | Page 3
helicase
synthetase
replicase
polymerase
64
exactly 20
exactly 21
exactly 24
at least 23
1,
3,
3,
1,
1
1
3
20
deoxyribose, ribose
ribose, deoxyribose
ribose, phosphate
ribose, uracil
10%
20%
40%
80%
34. DNA and RNA together are normally composed of how many kinds of
nucleotides?
Questions were edited & taken from Campbell and Farrell Biochemistry 7 th ed., www.biologycorner.com, and Prof.
Espanos MBB 10 slides.
V2 (10/12/2015) | Prepared by the UP Molecular Biology and Biotechnology Society | Page 4
a.
b.
c.
d.
7
3
4
5
anticodon
mRNA
ligase
amino acid
36. Okazaki fragments occur on the ___ and are bonded together by ______
a.
b.
c.
d.
37. How many different codons are possible using G, C, A, and U bases?
a.
b.
c.
d.
22
24
64
an infinite number
deoxynucleic acid
deoxyribonucleic acid
denatured ribonucleic acid
deoxoribonuclear acid
I only
II only
III only
I and III
II and III
Questions were edited & taken from Campbell and Farrell Biochemistry 7 th ed., www.biologycorner.com, and Prof.
Espanos MBB 10 slides.
V2 (10/12/2015) | Prepared by the UP Molecular Biology and Biotechnology Society | Page 5
40. During what phase of the cell cycle does DNA replication happen?
a.
b.
c.
d.
G1
S
M
G2
high fidelity
bidirectional
continuous
semi-conservative
1
3
1
3
amino acid
amino acids
protein
proteins
43. The processing of mRNA in eukaryotes requires, among others, 5 capping and
addition of a poly-A tail. What is the significance of this?
a.
b.
c.
d.
45. Name an important difference in the replication of circular DNA versus linear
double-stranded DNA.
a.
b.
c.
d.
Circular DNA needs fewer DNA polymerases due to its inherent circular nature
Linear DNA takes place slower and is more prone to errors in replication
Linear DNA needs more than one origin of replication
Only 2 replication forks are present in the replication of a circular DNA
The synthesis of an mRNA strand begins with the sequences in the promoter
The entire mRNA strand is encoded into a polypeptide chain
All proteins contain methionine at the beginning of their sequence
Transcription and translation occurs separately in eukaryotes
II. Identification. Write the absolute best answer to each of the following
questions.
1. It is the enzyme that promotes unwinding and separation of the 2 strands of DNA.
2. The enzyme that joins Okazaki fragments together.
3. What is the direction of elongation?
4. What kind of nucleic acid is the primer in replication made of?
5. What is the specific enzyme that allows elongation of DNA in the leading strand?
6. It is the repetitive sequence of DNA found at the ends of chromosomes to protect
the genes from being eroded.
III. Essay. Answer the following questions in an essay format (no longer than 10
sentences).
1. Explain briefly what happens to eukaryotic mRNA before it can be translated
to protein.
2. Would you expect mRNA or rRNA to be degraded more quickly in the cell?
Why?
3. A mutation that changes an alanine residue in a protein to an isoleucine leads
to a loss of activity. Activity is regained when a further mutation at the same
site changes the isoleucine to a glycine. Why?
Questions were edited & taken from Campbell and Farrell Biochemistry 7 th ed., www.biologycorner.com, and Prof.
Espanos MBB 10 slides.
V2 (10/12/2015) | Prepared by the UP Molecular Biology and Biotechnology Society | Page 7