Foods, Flavors & Fragrances

Comprehensive
Pesticide Residue
Analysis by LC/MS/MS

Figure 1 More than 280 pesticide residuesincluding difficult

polar speciesshow excellent peak shape and retention on a
3m Ultra Aqueous C18 column.
A: Pesticides in
positive ion mode

Using an Ultra Aqueous C18 Column

By Becky Wittrig, Ph.D., Global HPLC Specialist, and Andr Schreiber,
Ph.D., AB SCIEX

Easily resolve and quantify more than

280 pesticide species.
Use LC/MS/MS to reliably monitor difficult
polar and/or thermally unstable species.
Aqueous C18 phase offers optimal
selectivity and retention.

While the MS/MS detector allows for specific,

sensitive detection of the pesticide species, the LC
separation is still important to ensure the highest
quality data. Conventional C18 stationary phases
are typically used for pesticide monitoring, but
the selectivity and retention is poor for more polar
species. In contrast, Ultra Aqueous C18 columns
are ideal for multi-pesticide residue monitoring
methods. In Figure 1, the analysis of more than
280 pesticides using the 3m Ultra Aqueous C18
is shown. Optimized stationary phase selectivity

1.0

2.0

1.0

2.0

3.0
3.0

4.0
4.0

5.0

6.0

7.0Time (min.) 8.0

9.0

10.0

11.0

12.0

13.0

14.0 15.0 min.

LC_FF0490

5.0
5.0

6.0
6.0

7.0
8.0
7.0
8.0
Time, min

9.0
9.0

10.0
10.0

11.0
11.0

12.0
12.0

13.0
13.0

14.0
15.0
min.
14.0
15.0
LC_FF0490A

5.0

6.0

7.0

8.0

9.0

10.0

11.0

12.0

13.0

14.0

15.0

B: Pesticides in
negative ion mode

0.0

1.0
1.0

2.0
2.0

3.0
3.0

4.0
4.0

propamocarb

C: Improved retention of
difficult pesticides in
positive ion mode

omethoate

With recent advances in LC/MS/MS instrumentation, this technique is quickly gaining acceptance for pesticide residue testing. LC/MS/MS can
be used to simultaneously monitor hundreds of
potential contaminantsincluding those difficult
to detect by GC. Using both LC/MS/MS and GC
approaches allows for a faster, more complete
picture of pesticide residues. MS/MS technology
also permits identification of the target pesticides
through the selection of specific MRM transitions
for each compound. For example, aldicarb, a carbamate pesticide, uses two MRM transitions of
208.2g89.1amu and 208.2g116.1amu.

methamidophos
acephate

Food safety is a topic of great interest globally. With recent contamination issues in a wide
range of commodities, ensuring the quality of our
food supply is becoming increasingly important.
Pesticide residue content is one area of concern.
While pesticides have typically been monitored
by gas chromatography, polar and/or thermally
unstable pesticides are difficult or impossible to
monitor using this approach. Thus, traditional
HPLC techniques are used for select pesticide
classes, such as the carbamate and phenylurea
pesticides.

0.0

0.0

1.0
1.0

2.0
2.0

3.0
3.0

4.0
4.0

See next page for conditions.

5.0
5.0

6.0
6.0

7.0 Time (min.) 8.0

7.0

8.0

9.0
9.0

10.0
10.0

11.0
11.0

12.0
12.0

13.0
13.0

14.0
14.0

15.0 min.
15.0

LC_FF0490B

Innovative Chromatography Solutions

www.restek.com

Conditions for Figure 1 (previous page):

Sample:
Inj.:
Conc.:
Sample diluent:

multicomponent pesticide standard

10L
1ppb each pesticide
water

Column:
Cat. #:
Dimensions:
Particle size:
Pore size:

Ultra Aqueous C18

9178312
100mm x 2.1mm
3m
100

Conditions:
Instrument:
Mobile phase:

Flow:
Temp.:
Det.:
Ion Source:
IonSpray Voltage:
Gas 1:
Gas 2:
Source Temp.:

Ultra-high pressure LC (UHPLC) can also be used with MS/MS detection for
monitoring pesticide residues. UHPLC allows for higher sample throughput
when used in conjunction with a highly efficient <2m particle size column.
The 1.9m Pinnacle DB Aqueous C18, in a 50 x 2.1mm configuration, is
ideally suited for this application, as shown in Figure 2.

Shimadzu Prominence UFLCXR

A: 10 mM NH4OAc in water
B: 10 mM NH4OAc in methanol
Time (min.)
0.0
8.0
12.0
14.8
14.9

allows for an even distribution of the compounds throughout the retention

time window (Tables I and II). As well, retention of more polar pesticides
is greatly improved, as demonstrated in Figure 1C. The Ultra Aqueous C18
column, in a 100 x 2.1mm, 3m configuration is the column of choice for LC/
MS/MS pesticide monitoring methods.

%B
20
90
100
100
20

Figure 2 Higher sample throughput can be achieved using

UHPLC and MS/MS with a 1.9m Pinnacle DB Aqueous C18
column.

500L/min
35C
Applied Biosystems 4000 QTRAP
LC/MS/MS system
TurboIonSpray
A & C: ESI+
B: ESI5kV (ESI+), -4.2kV (ESI-)
50psi
60psi
600C

Table I Peak list for pesticides in negative

ion mode.
Retention
Compound ID Time (min)
Acrinathrin
9.09
Chlorfluazuron
9.24
Clothianidin
3.63
Diflubenzuron
7.68
Diuron
6.78
Fluazinam
7.99
Fludioxonil
6.93
Forchlorfenuron
6.44
Hexaflumuron
8.45
Imibenconazol
8.82
Lufenuron
8.87
Metaflumizon
8.5
Metamitron
3.97
Methoxyfenozid
6.98
Nitenpyram
2.59
Novaluron
8.42
Profoxydim
7.57
Propoxycarbazon
3.09
Prothioconazol
7.16
Tebufenozid
7.46
Teflubenzuron
8.85
Tepraloxydim
4.57
Terbacil
5.38
Tralkoxydim
6.28
Triadimefon
7.01
Triflumuron
8

Transition 1
540 g 372
539.9 g 356.8
249.9 g 58
309 g 156.1
230.9 g 185.8
462.5 g 415.8
246.9 g 179.9
246 g 126.9
459 g 438.8
409 g 250.9
509 g 325.9
505.1 g 301.9
201 g 184.8
366.9 g 104.9
269.2 g 221.6
491.1 g 470.7
464.3 g 277.9
396.9 g 156
342 g 100.1
350.9 g 149
378.6 g 338.8
340 g 248
214.9 g 158.9
328.1 g 253.8
292 g 67.9
356.9 g 153.8

Transition 2
540 g 299.9
539.9 g 519.7
247.9 g 59
310 g 288.9
230.9 g 149.8
462.5 g 397.9
246.9 g 125.9
246 g 91.2
459 g 174.9
411 g 253
509 g 175
505.1 g 140.9
201 g 116.9
366.9 g 148.9
269.2 g 100.8
493.1 g 472.7
464.3 g 126.8
396.9 g 112.9
343.9 g 99.9
350.9 g 105
378.6 g 195.9
340 g 220.1
216.9 g 160.9
328.1 g 281.8
292 g 234.9
358.9 g 155.9

LC_EV0479A

Compounds analyzed are a subset of those

in Figure 1; data are shown for a qualitative
overall run time comparison only.

Conditions:
Mobile phase:

Sample:
multicomponent pesticide
standard
Inj.:
5L
Conc.:
33.3ppb each pesticide
Sample diluent: water
Column:
Cat.#:
Dimensions:
Particle size:
Pore size:

Pinnacle DB Aqueous C18

9418252
50mm x 2.1mm
1.9m
140

A: 10mM NH4OAc in water

B: 10mM NH4OAc in methanol
Time (min.)
0.0
1.0
8.0
10.0
11.0

Flow:
Temp.:
Instrument:
Det.:

%B
10
10
90
90
10

600L/min.
35C
Shimadzu Prominence UFLCXR
Applied Biosystems 4000 QTRAP
LC/MS/MS system
Ion Source: TurboIonSpray, ESI+
IonSpray Voltage, ESI voltage: 5kV (ESI+)
Gas 1: 40psi
Gas 2: 60psi
Source Temp.: 500C;
max pressure ~7,200psi

Using LC/MS/MS technology and Aqueous C18 columns, in combination

with gas chromatography, results in the most comprehensive monitoring of
pesticide residues. Labs interested in more complete multi-residue analysis
of pesticides in food matrices, including difficult polar or thermally unstable
compounds, should consider adding LC/MS/MS and Aqueous C18 columns
to routine testing procedures. The Aqueous C18 phase is also available on
1.9m Pinnacle DB silica for UHPLC platforms.
Acknowledgements

The authors wish to thank the US FDA for their collaboration and recognize
the participation of multiple FDA labs in this work.

Restek Corporation

1-800-356-1688 or 1-814-353-1300 www.restek.com

Table II Peak list for pesticides in positive ion mode (continued on next page).
Compound ID
Acephate
Acetamiprid
Acibenzolar-S-methyl
Alanycarb
Aldicarb
Aldicarbsulfone
Aldicarbsulfoxide
Ametryn
Aminocarb
Avermectin B1a
Avermectin B1b
Azoxystrobin
Benalaxyl
Bendiocarb
Benfuracarb
Benthiavalicarb
Benthiavalicarb-isopropyl
Benzoximate
Bitertanol
Boscalid
Bromuconazole (isomer 1)
Bromuconazole (isomer 2)
Bufencarb
Bupirimate
Buprofezin
Butafenacil
Butocarboxim
Butocarboximsulfoxid
Butoxycarboxim
Carbaryl
Carbendazim
Carbetamide
Carbofuran
Carboxine
Carfentrazone-ethyl
Chlorfluazuron
Chloroxuron
Chlorpyrifos
Chlorpyrifos-methyl
Chlortoluron
Cinidon-ethyl
Clethodim
Clofentezine
Clomazone
Clothianidin
Cyanazine
Cyazofamid
Cycluron
Cymoxanil
Cyproconazole (isomer 1)
Cyproconazole (isomer 2)
Cyprodinil
Cyromazine
Demeton-S-methyl
Demeton-S-methyl-sulfon
Desethyl-atrazine
Desisopropyl-atrazine
Desmedipham
Desmethyl-pirimicarb
Diazinon
Dichlorvos
Diclobutrazol
Dicrotophos
Diethofencarb
Difenoconazole (isomer 1)
Difenoconazole (isomer 2)
Difenoxuron
Diflubenzuron
Dimethenamide
Dimethoate
Dimethomorph
Dimoxystrobin
Diniconazole
Dinotefuran
Dioxacarb
Diphenylamin
Disulfoton
Diuron
Edifenphos

Retention
Time (min)
1.27
4.44
7.22
7.91
4.49
2.03
2.2
6.96
5.37
11.2
11.4
6.78
7.78
5.1
8.33
6.87
6.87
7.98
7.98
6.98
7.36
8.05
5.18
7.65
8.53
7.08
4.4
2.1
1.95
5.63
4.52
4.74
5.18
5.61
7.53
9.18
7.53
8.35
7.88
6.31
8.71
5.81
8.27
7.04
3.35
5.51
7.4
6.71
3.91
7.45
7.44
8.15
2.63
2.9
5.6
3.9
3.2
6.23
5.11
7.88
5.36
7.58
3.47
6.56
8.35
8.35
6.78
7.62
6.9
3.58
7.22
7.6
8.07
2.02
3.68
7.03
6.08
6.7
6.66

www.restek.com

Transition 1
184.1 g 143
223.2 g 126.1
211 g 136
400.1 g 238.2
208.2 g 116.1
223.1 g 86.1
207.1 g 132.1
228.1 g 186.1
209.1 g 137.1
890.5 g 305
876.5 g 291
404.1 g 372.1
326.2 g 148.2
224.2 g 109.2
411.2 g 195.1
382.1 g 116
382.1 g 196.8
364 g 199
338 g 70
343 g 307
378 g 159.1
378.1 g 159.1
222.2 g 95.1
317 g 166
306.2 g 201.1
492.2 g 331.1
191.1 g 75
207 g 74.9
223.1 g 106
202.1 g 145
192.2 g 160.2
237 g 192
222.2 g 123.1
236 g 143
412 g 346
540 g 158
291 g 72
350 g 198
324 g 125.1
213.1 g 72.2
394.1 g 348.1
360 g 164
303 g 138
240.1 g 125
250 g 169.1
241.2 g 214.2
325 g 108
199.1 g 89.1
199 g 128
292.2 g 70.2
292.1 g 70.2
226 g 93
167.2 g 85.1
230.9 g 89.1
262.9 g 108.9
188.1 g 146.2
174.1 g 104.1
318 g 182
225 g 72
305.1 g 169.2
221 g 109.1
328 g 70
238 g 112
268 g 226
406.2 g 251.1
406.1 g 251.1
287.2 g 123.2
311 g 158.2
276.2 g 244.1
230 g 125
388.2 g 301.1
327.1 g 205
326 g 70
203.1 g 114.1
224 g 123
170.1 g 93
275.1 g 89
233.1 g 72
311 g 283

Transition 2
184.1 g 125
223.2 g 99.1
211 g 140
400.1 g 91.1
208.2 g 89.1
223.1 g 148
207.1 g 89.1
228.1 g 96
209.1 g 152.1
890.5 g 145
876.5 g 145
404.1 g 344.1
326.2 g 91.1
224.2 g 167.2
411.2 g 252.1
382.1 g 180.1
382.1 g 179.9
364 g 105
338 g 269
343 g 140
378 g 161
378.1 g 161
222.2 g 71
317 g 108
306.2 g 116.2
492.2 g 180.1
191.1 g 116
207 g 90
223.1 g 166
202.1 g 127
192.2 g 132.1
237 g 118
222.2 g 165.2
236 g 87
412 g 366
540 g 383
291 g 218
350 g 96.9
322 g 125.1
213.1 g 46.2
394.1 g 107
360 g 268
303 g 102
240.1 g 89.1
250 g 132
241.2 g 104.1
325 g 261
199.1 g 89
199 g 111
292.2 g 125.2
292.1 g 125.2
226 g 77
167.2 g 68.25
230.9 g 61
262.9 g 169
188.1 g 104.1
174.1 g 96.1
318 g 136
225 g 168.1
305.1 g 97
223.1 g 109.1
328 g 160
238 g 193
268 g 180
408.2 g 253.1
408.1 g 253.1
287.2 g 72
311 g 141.2
276.2 g 168.3
230 g 199.1
388.2 g 165.2
327.1 g 116
326 g 159
203.1 g 129
224 g 167.1
170.1 g 92
275.1 g 61
235.1 g 72.1
328 g 283

Compound ID
EPN
Epoxiconazole
Etaconazole
Ethiofencarb
Ethiofencarbsulfoxid
Ethion
Ethiprole
Ethofenprox
Ethofumesate
Ethoprophos
Ethoxyquin
Etoxazole
Famoxadone
Fenamidone
Fenamiphos
Fenarimol
Fenazaquin
Fenbuconazole
Fenhexamid
Fenitrothion
Fenoxycarb
Fenpropimorph
Fenpyroximate
Fenthion
Fenuron
Flonicamid
Flucarbazone
Flufenacet
Flufenoxuron
Fluometuron
Fluopicolid
Fluoxastrobin
Fluquinconazole
Flusilazole
Flutolanil
Flutriafol
Fonophos
Forchlorfenuron
Formetanate
Fuberidazole
Furalaxyl
Furathiocarb
Hexaconazole
Hexaflumuron
Hexythiazox
Hydramethylnon
Imazalil
Imazapyr
Imidacloprid
Indoxacarb
Ipconazole
Iprodion
Iprovalicarb
Irgarol
Isofenphos
Isoprocarb
Isoproturon
Isoxaflutole
Kresoxim-methyl
Lenacil
Linuron
Lufenuron
Malathion
Mandipropamid
Mefenacet
Mepanipyrim
Mepronil
Metalaxyl
Metconazole
Methabenzthiazuron
Methamidophos
Methiocarb
Methomyl
Methoprotryne
Methoxyfenozide
Metobromuron
Metolachlor
Metoxuron
Metribuzin

Retention
Time (min)
6.72
7.65
7.57
5.58
3.48
7.9
6.62
9.66
6.54
5.98
7.4
9
7.72
6.65
7.5
7.3
9.91
7.57
7.04
6.4
7.53
9.84
9.29
6.9
3.85
2.25
2.81
7.17
8.98
5.98
7.2
7.34
7.31
7.6
6.81
5.99
5.9
6.37
4.48
5.5
6.62
8.42
7.95
8.42
8.77
9.2
8.1
7.3
3.82
8.03
8.33
7.6
6.98
7.72
6.66
5.87
6.42
5.87
7.53
6.5
6.99
8.83
6.83
6.81
7.35
7.57
6.86
6.13
8.01
6.56
1.06
6.82
2.62
6.95
6.9
6.22
7.5
5.1
5.14

Transition 1
324 g 157.1
330 g 121
328.2 g 159.1
226.1 g 106.9
242 g 107
385 g 199
397.3 g 351
394.1 g 107.1
304 g 121
243 g 131
218.1 g 174
360.1 g 141
392 g 331
312.1 g 92.1
304.2 g 217.1
331 g 268
307 g 161
337 g 125
302 g 97
278 g 125.2
302.2 g 88.1
304 g 147
422 g 366.1
278.9 g 169
165.1 g 72.1
230.1 g 203.1
397.1 g 130.1
364.1 g 152.2
489.1 g 158.2
233.1 g 72.1
385 g 174.8
459.2 g 427.2
376 g 349
316 g 247
341.1 g 242.1
302 g 123
247 g 183
248 g 93.1
222 g 165.1
185 g 157
302.1 g 95.1
383.2 g 195.2
314 g 70
461.1 g 158.2
353 g 228
495.2 g 323.1
297.1 g 159.2
262.2 g 217.2
256.2 g 209
528 g 203
334.2 g 70
330.1 g 244.9
321.2 g 119
254.2 g 198.1
314 g 120
194.2 g 95.1
207.2 g 72.1
360.1 g 251.1
314 g 116
235.3 g 153.2
249.1 g 160
511.1 g 158.1
331 g 99.1
412.1 g 328.1
299 g 148.1
224 g 106
270.1 g 119.1
280.2 g 220.2
320 g 70
222.1 g 165.2
142 g 94
226.1 g 169.2
163.1 g 88.1
272.2 g 240.2
369 g 149
259 g 170.2
284.2 g 252.2
229 g 72.1
215.1 g 187.2

Transition 2
324 g 296
330 g 101
328.2 g 123
226.1 g 164.1
242 g 185.1
385 g 171
397.3 g 255.5
394.1 g 177.2
304 g 161
243 g 97
218.1 g 160
360.1 g 57.2
392 g 238
312.1 g 65
304.2 g 202.1
331 g 81
307 g 147
337 g 70
302 g 55
278 g 109
302.2 g 116.2
304 g 117
442 g 135.1
278.9 g 246.9
165.1 g 46
230.1 g 174
397.1 g 115
364.1 g 194.2
489.1 g 141.2
233.1 g 46
383 g 173
459.2 g 188
376 g 307
316 g 165
341.1 g 262.1
302 g 109
247 g 201
248 g 165.1
222 g 120
185 g 65
302.1 g 242.1
383.2 g 252.2
314 g 159
461.1 g 141.1
353 g 168
495.2 g 151
299.1 g 161.2
262.2 g 202.2
256.2 g 175.2
528 g 56
334.2 g 125
332.1 g 247
321.2 g 203.2
254.2 g 83.2
314 g 162.1
194.2 g 137.2
207.2 g 46.1
360.1 g 220.1
314 g 206
235.3 g 136.2
249.1 g 182.1
511.1 g 141.2
331 g 127.1
412.1 g 355.9
299 g 120.1
224 g 77
270.1 g 228
280.2 g 192.3
320 g 125
222.1 g 150.3
142 g 125
226.1 g 121.1
163.1 g 106
272.2 g 198
369 g 133
259 g 148.2
284.2 g 176.2
229 g 156.1
215.1 g 84.1

1-800-356-1688 or 1-814-353-1300 Restek Corporation

Table II Peak list for pesticides in positive ion mode (continued from previous page).
Retention
Compound ID
Time (min)
Mevinphos
4.29
Mexacarbate
7.02
Milbemectin A3
10.3
Milbemectin A4
10.5
Molinate
7.3
Monocrotophos
2.9
Monolinuron
5.93
Monuron
5.7
Myclobutanil
7.17
Neburon
7.65
Nitenpyram
2.55
Novaluron
8.38
Nuarimol
6.7
Omethoate
1.69
Oxadixyl
4.85
OxamyI
2.35
Oxydemeton-methyl
3.1
Paclobutrazol
6.82
Parathion-ethyl
6.7
Parathion-methyl
7.6
Penconazole
8.01
Pencycuron
8.1
Phenmedipham
6.35
Phenthoate
7
Phosmet
6.7
Phoxim
7.9
Picoxystrobin
7.44
Pinoxaden
7.99
Piperonyl butoxide
8.62
Pirimicarb
6.29
Pirimicarb-desmethylformamido
6.4
Pirimiphos-ethyl
7.48
Prochloraz
8.29
Promecarb
6.86
Prometon
6.86
Prometryn
7.4
Propachlor
6.2
Propamocarb
6.61
Propargite
8.79
Propazine
6.9
Propham
5.78
Propiconazole
7.98
Propoxur
5.03
Prosulfocarb
8.5
py Cinerin I
9
py Cinerin II
8.2
Pymetrozin
3.61
Pyracarbolid
5.4
Pyraclostrobin
7.95
Pyrazophos
7.8
Pyrethrin I
8.9

Transition 1
225 127
223.2 166.2
546.4 511.3
560.4 525.4
188.2 126.2
224 127
215.1 126.1
199.2 72.2
289 70
275 88
271.2 126.1
493 158.1
315 252
214 124.9
279.2 219.2
237.1 72.1
247 169
294 70
292.1 236.2
263.9 232.1
284 159
329.1 125.1
301.1 136
321 163
318 160
299.1 129.1
368 145
401.3 317.2
356.2 177.2
239.2 72.1
253.2 72.1
334 198.1
376.1 308
208.2 109.1
226.1 142.1
242.2 200.1
212.2 170.1
189.2 102.2
368 231
230.1 146.1
180 138
342.1 159.1
210.1 111
252.3 91.1
317.2 149
361.2 149
218 105
218.2 125
388 194
374 222
329.2 160.9

Transition 2
225 193
223.2 151
546.4 493.3
560.4 55.2
188.2 55.1
224 98
215.1 99
199.2 126.3
289 125
275 114
271.2 237.2
493 141.1
315 81
214 182.8
279.2 132.1
237.1 90.1
247 109
294 125
292.1 94.1
263.9 125
284 70
331.2 127
301.1 168.1
321 79
318 133
299.1 77.1
368 205
401.1 57
356.2 119
239.2 182.2
253.2 225.3
334 182.1
376.1 70.1
208.2 151.1
226.1 86
242.2 158.1
212.2 94.1
189.2 73.9
368 175
230.1 188.1
180 120
342.1 69.1
210.1 168.1
252.3 128.1
317.2 106.9
361.2 106.9
218 78
218.2 97
388 163
374 194
329.2 132.9

Compound ID
Pyrethrin II
Pyridaben
Pyridaphenthion
Pyrimethanil
Pyriproxyfen
Quinalphos
Quinoxyfen
Rotenone
Secbumeton
Siduron
Simetryn
Spinosyn A
Spinosyn D
Spirodiclofen
Spiromesifen
Spiroxamine (isomer 1)
Spiroxamine (isomer 2)
Sulfentrazone
Sulfotep-ethyl
Sulprofos
Tebuconazole
Tebufenozide
Tebufenpyrad
Tebuthiuron
Teflubenzuron
Terbufos
Terbumeton
Terbutryn
Tetraconazole
Thiabendazole
Thiacloprid
Thiamethoxam
Thidiazuron
Thiobencarb
Thiofanox
Thiofanoxsulfon
Thiofanoxsulfoxid
Thiophanate-methyl
Tolclofos-methyl
Topramezone
Triadimefon
Triadimenol
Tricyclazole
Trifloxystrobin
Triflumizole
Triflumuron
Triticonazole
Uniconazole
Vamidothion
Zoxamide

Retention
Time (min)
8.3
9.33
7.8
7.24
8.72
6.7
9.12
7.61
6.85
6.55
6.36
11.3
11.6
8.96
8.8
10.7; 11
10.7; 11
4.77
7
7
7.8
7.39
8.56
5.71
8.81
6.5
6.84
7.57
7.3
5.71
4.89
3.06
5.18
8.09
5.7
3.4
3.6
5.1
8
1.73
6.94
7.04
5.18
8.09
8.47
7.94
7.38
7.4
3.75
7.7

Transition 1
373.1 160.9
365 147
341 189
200 107
322 96
299 147
308 197
395 213
226.2 170.1
233.3 137.2
214 124
732.6 142.1
746.6 142.1
411.3 313.2
371.3 273
298.4 144.2
298.3 144.2
387 307.1
323 115
323 219
308 70
353.1 133.1
334 117
229.2 172.4
381.1 141.2
289 103
226 170
242.2 186.1
372 159
202.1 175.1
253.1 126.1
292 211
221.2 102.1
258.1 125
219 57.1
251.1 75.9
235.1 104.1
343 151
301 175
364.1 334.1
294 197
296.1 70.1
190 163
409 186
346 278
359.1 156.2
318 70
292.2 70.1
288 146
336.1 186.9

HPLC Columns (USP L1)

Pinnacle DB Aqueous C18 Columns

Ultra Aqueous C18 Columns

pore size: 100
carbon load: 15%
endcap: no
3m Column, 2.1mm
100mm
100mm (with Trident Inlet

pH range: 2.5 to 8
temperature limit: 80C
cat. #
price
9178312
9178312-700

For guard cartridges for these columns, visit www.restek.com

pore size: 140

carbon load: 6%
1.9m Column, 2.1mm
50mm

pH range: 2.5 to 8
temperature limit: 80C
cat. #
price
9418252

Transition 2
373.1 308.9
365 309
341 205
200 82
322 185
299 163
308 162
395 192
226.2 100
233.3 94
214 144
732.6 98
746.6 98
411.3 213.1
371.3 255
298.4 100.2
298.3 100.2
387 146
323 171.1
323 247
308 125
353.1 297.1
334 145
229.2 116.1
381.1 158.2
289 57
226 114
242.2 68.1
372 70
202.1 131.2
253.1 99.1
292 181
221.2 127.9
258.1 89
219 60.9
251.1 57
235.1 57
343 192
301 268.9
364.1 125
294 225
296.1 227.2
190 136
409 206
346 73
359.1 139
318 125
292.2 43
288 118
338.1 188.7

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