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Hidrogen
Functions
Component
molecules
of
water
and
Karbon
Nitrogen
Sulphur
Sulfur
Component
vitamins.
of
certain
Calcium
Kalsium
proteins
and
Ultratrace
element
Carbon
Oksigen
Potassium
organic
Oxygen
Unsur adalah unis asas jirim. Antara 92 unsur yang dijumpai di bumi, hanya 25
dijumpai dalam benda hidup.
Element
Hydrogen
Klorin
Kalium
Category
Major
constituents
Chlorine
Iron
besi
Unsur-unser
ultrasurih
Organic compound
Carbohydrates
Karbohidrat
Function
Supply energy for cell processes, storing energy
and give structural support to cell wall.
Membekal tenaga untuk proses sel, menyimpan tenaga dan
memberi sokongan kepada dinding sel.
Lipids
Lipid
Proteins
Nucleic acids
Asid nukleik
Terdiri daripada polinukleotida yang berpilin bersama untuk membentuk struktur keliks
ganda dua.
Water is not easily compressed, helps to give the cells its shape and
structure. (Support)
genetic substances for some virus. (Bahan genetik bagi sesetengah virus)
Carbohydrates (karbohidrat):
- made up of carbon, hydrogen and oxygen. The ratio of hydrogen to
oxygen atoms in the molecule is usually 1:2.
Monosaccharide (Monosakarida)
- also called simple sugar (gula ringkas)
-
Terdiri daripada karbon, hidrogen dan oksigen dalam nisbah hidrogen kepada
oksigen ialah 1:2.
Carbohydrate
Monosaccharides
(simple sugars)
Disaccharides
(double sugars)
Polysaccharides
Disaccharides (Disakarida)
- Formed from two monosaccharides molecules combining together
with the elimination of a molecular of water. The chemical reaction
of the formation is known as condensation.
Formula
C6H12O6
C6H12O6
C6H12O6
C12H22O11
lactose
maltose
starch
C12H22O11
C12H22O11
-(C6H10O5)-
Source
fruits
fruits, honey
not naturally occurring
sugar cane, sugar beet,
fruits
milk
germinating grain/seed
energy store in plants
glycogen
Name
glucose
fructose
galactose
sucrose
-(C6H10O5)n
cellulose
-(C6H10O5)-
Name
Formula
glucose
C6H12O6
galactose
C6H12O6
fructose
C6H12O6
Structure
Name
sucrose
Formula
(glucose + fructose)
Structure
lactose
Maltose
(glucose
galactose)
Polysaccharide
Starch
(Kanji)
subunit
Glucose
(glucose + glucose)
Structure
Consists
of
2
components,
unbranched, helical
chains of glucose
unit and branched
chain of glucose
unit.
Occurance
Major storage of
carbohydrate in
plants.
(Karbohidrat
simpanan
dalam
tumbuhan)
Mengandungi
dua
kompenen ialah tidak
bercabang
(rantaian
heliks glukosa), dan
bercabang.
Polysaccharides (Polisakarida)
- Many monosaccharide molecules can be added by a series of
condensation reactions to form a very large molecule
(macromolecule).
Banyak molekul monosakarida boleh digabungkan dengan tindaka balas
kondensasi untuk membentuk molekul besar.
Glycogen
(Glikogen)
Glucose
Highly
branched Major storage of
short
chains
of carbohydrate in
glucose units.
animals
and
Rantaian
glukosa fungi.
bercabang yang pendek.
Cellulose
(Selulosa)
Glucose
Karbohidrat
simpanan
dalam
haiwan dan kulat.
Sugars that give a negative Benedicts test are known as nonreducing sugars. Example of non-reducing sugar is sucrose.
Gula yang tidak memberi kesan terhadap Ujian benedict dipanggil gula bukan
penurun. Contoh gula bukan penurun ialah sukrosa.
Results:
Solution
Glucose
Fructose
Galactose
Maltose
Sucrose
Lactose
Conclusion:
Benedicts test
Brick-red precipitate
Brick-red precipitate
Brick-red precipitate
Brick-red precipitate
Blue solution
Brick-red precipitate
Method B:
1. 2ml of sucrose solution are poured into a test tube.
2. 1ml of dilute hydrochloric acid is added to the test tube.
3. The test tube is placed in a water bath and boiled for 5 minutes.
4. The test tube is cooled under a tap and solid sodium hydrogen carbonate is
added to neutralise the excess acid.
5. The test tube is placed in a water bath and boiled for 5 minutes.
5. Each amino acid carried two functional groups: a carboxyl group (COOH) which is acidic and an amino group (NH2) which is basic.
Setiap asid amino mempunyai dua kumpulan berfungsi: kumpulan karboksil (COOH) yang bersifat asid dan kumpulan amino (NH2) yang bersifat bes.
Struktur sekunder
Tertiary
structure
Struktur tertier
Enzim, hormon, antibodi dan protein plasma ialah contohcontoh protein kompleks struktur tertier.
Manusia boleh mensintesis 11 asid amino dan dipanggil asid amino tidak perlu.
4. Animal protein refer as first class protein while plant protein refer as
second class protein.
Protein haiwan ialah protein kelas pertama manakala protein tumbuhan ialah protein kelas
kedua.
Protein structure
Primary
structure
Struktur primer
Description
The linear sequence of amino acids in structure a
protein molecule. The first protein to have its
sequence of amino acids determined was the hormone
insulin.
Urutan linear asid amino dalam satu rantai polipeptida.
Secondary
structure
Quaternary
structure
Struktur Quartener
4.
5.
c.
d.
Lipids (Lipid)
1. Lipids are diverse group of substances that contain carbon, hydrogen
and oxygen. The proportion of oxygen is lower than that in
carbohydrates. For example, the general formula of stearic acid is
C18H36O2 or C17H35COOH
Lipid terdiri daripada karbon, hidrogen dan oksigen.Nisbah atom hidrogen
kepada atom oksigen dalam molekul lipid adalah jauh lebih tinggi daripada
nisbah 2:1 dalam karbohidrat.
2.
All lipids are insoluble in water. Semua lipid tidak terlarut dalam air.
3.
e.
f.
g.
6.
Waxes (lilin)
a. Waxes are similar to triglycerides, but the fatty acids are
bonded to long chain alcohols rather than glycerol.
Asid lemak lilin terikat kepada rantai panjang alkohol.
b.
c.
d.
7.
8.
Steroids
a. A steroid molecule has a complex ring structure.
Steroid merupakan lipad yang terdiri daripada molekul berbentuk
cincin yang kompleks
Phospholipids (Fosfolipid)
a. Phospholipids have a similar structure to triglycerides but
one of the fatty acids is replaced by a phosphate group.
b.
c.
b.
Steroids
Cholesterol
Testosterone
Differences (perbezaan)
Hormon lelaki
Oestrogen and
progesterone
Female reproductive hormone
Hormon perempuan
More likely to cause diseases of the Less likely to cause diseases of the
heart and arteries
heart and arteries
Lebih
cenderung
menyebabkan
penyakit jantung dan arteri
Vegetable oils such as olive oils, corn oil and sunflower oil
are examples of unsaturated fats.
Minyak sayur seperti minyak zaitun, jagung dan bunga matahari ialah
lemak tidak tepu.
Similarities (persamaan)
-
No double bond and can not react Has double bond and can react with
with hydrogen.
hydrogen.
Tiada ikatan ganda dua dan tidak boleh
bertindak balas dengan hidrogen.
Enzyme (Enzim)
- Enzymes are proteins which act as biological catalysts. They
speed up biochemical reactions in the cell.
Enzim ialah protein yang digunakan sebagain mangkin biologi. Mereka
mempercepatkan tandak balas biokimia dalam sel.
Sesuatu tindak balas metabolisme bermula dengan molekul substrat dan berakhir
dengan hasil.
arrows.
Dalam sel, tindak balas biokimia mestilah berlaku dengan kadar yang pantas supaya
proses hidup sentiasa dipelihara
Enzim disintesis oleh sel-sel tertentu.Enzim yang tidak dirembes ke luar sel tetapi
dikekalkan untuk kegunaan dalam sel dinamakan enzim intrasel.Enzim intrasel terdapat
dalam sitoplasma, nukleus, kloroplas atau membran plasma.Enzim oksidoreduktase
yang terdapat dalam organel mitokondrion ialah enzim yang memangkinkan respirasi
sel.Contoh lain seperti DNA polimerase, RNA polimerase, dan ATP synthetase.
Extracellular enzymes are produced in the cell, then packed and secreted
from the cell. Extracellular enzymes catalyst their reactions outsides the
cell. Most digestive enzymes are extracellular enzymes. For example,
amylase, cellulose and zymase.
o
enzim yang disintesis dalam sel dan kemudiannya dirembes keluar untuk bertindak di
luar sel disebut enzim luar sel. Contoh: amilase, selulase dan zimase.
o
Ribosom didapati terlekat pada jalinan endoplasma kasar atau bebas dalam
sitoplasma.Maklumat untuk sintesis enzim terkandung dalam molekul DNA
nukleus. Urutan bes bernitrogen pada DNA yang berlainan menggekodkan
protein yang berbeza.Dalam proses, bebenang tunggal molekul RNA dibentuk
untuk menterjemah kod-kod tersebut menjadi satu urutan asid amino.
Problem:
What is the effect of temperature on enzyme-catalysed reactions?
Hypothesis: The enzyme is inactive at low temperatures. It is most active
0
20
37
50
60
>30
8
3
6
>30
<0.03
0.13
0.33
0.17
<0.03
Discussion:
1. Test tube P and Q are kept in the water bath for five minutes to
uniform the temperature of the solutions in the two test tubes.
2. When the amylase in the saliva has completely hydrolysed the
starch, the dark blue colour does not form in the iodine test.
Conclusion:
At suboptimum temperatures, increasing the temperature increased the
reaction rate until the optimum temperature is reached at 37C. Above the
optimum temperature, the reaction rate fell rapidly because the enzyme is
denatured.
___________________________________________________________
Aim: Investigate the effect of pH on enzyme-catalysed reaction
Problem: What is the effect of pH on enzyme-catalysed reactions?
Hypothesis: The enzyme pepsin works best in acidic conditions?
Manipulated variable: pH
Responding variable: reaction rate
Fixed variable: Volume and concentration of egg albumen suspension,
volume and concentration of pepsin, temperature of the
solution.
Materials: Egg albumen suspension, dilute hydrochloric acid, dilution
sodium hydroxide and 1% pepsin.
Apparatus: Beaker, tripod stand, wire gauze, Bunsen burner,
thermometer,
dropper, test tube and measuring cylinder
Method:
1. The egg albumen suspension is prepared by adding 1g fried egg
albumen to 100ml of water. The mixture is then heated to 90C.
2. Three test tubes are labelled P, Q and R respectively.
3. 5ml of egg albumen suspension and 1ml of pepsin are placed into
each test tube.
4. 5 drops of distilled water added to test tube P.
5. 5 drops of dilute hydrochloride acid are added to test tube Q.
6. 5 drops of dilute sodium hydroxide are added to test tube R.
7. All the three test tubes are placed into a water bath at 37C for 15
minutes.
8. The appearance of the contents in each tube is recorded at the
beginning and at the end of the activity.
Results:
Test tube Contents
Appearance of content
Beginning
End
P
Albumen + pepsin + White
White
distilled water
suspension
suspension
Q
Albumen + pepsin + White
Clear suspension
dilute hydrochloric acid suspension
R
Albumen + pepsin +
White
White
dilute sodium
suspension
suspension
hydroxide
Discussion:
1. The mixture in test tube Q is clear at the end of the activity because
the albumen has been hydrolysed.
2. Pepsin do not catalyse the hydrolysis of albumen in neutral and in
alkaline conditions.
Conclusion:
Pepsin catalyses the hydrolysis of protein in acidic conditions. The
hypothesis is accepted.
Mechanism of enzyme action (Mekanisme tindakan enzim)
o Each enzyme molecule has a region with very precise shape called
the active site.
Setiap molekul enzim mempunyai satu bahagian yang mempunyai bentuk tetap
dipanggil tapak aktif.
o
The substrate molecule fits into the active site of the enzyme like a
key into a lock.
Molekul substrat bergabung dengan tapak aktif pada enzim seperti mangga
dan kunci.
o
atau
Once formed, the products no longer fit into the active site and
escape into the surrounding medium, leaving the active site free to
receive further substrate molecules.
Selepas perubahan bentuk substrat, bentuknya tidak lagi bersesuaian dengan
tapak aktif dan hasil itu meninggalkan enzim. enzim tersebut bebas bertindak
dengan substrat lain.
Mekanisme tindakan enzim yang spesifik dikenali sebagai hipotesis mangga dan kunci
kerana penggabungan substrat kepada enzim dapat dianalogikan sebagai kunci dam
mangga.
o
Temperature (Suhu)
Initially an increase in temperature leads to an increase in
the rate of reaction because the kinetic energy of the
enzyme and substrate molecules produces more
collisions and therefore more enzyme-substrate
complexes are formed.
Peningkatan suhu pada peringkat awal meningkatkan kadar tindak
balas kerana peningkatan tenaga kinetik enzim dan substrat
menghasilkan lebih banyak pelanggaran. Oleh itu, lebih banyak
kompleks enzim-substrat terbentuk.
Eventually all the active sites are filled at any one time
and the rate remains constant. The reaction has reached
its maximum rate, Vmax.
Pada satu peringkat, semua tapak aktif telah digunakan (mencapai
takat tepu) dan kadar tindak balas menjadi malar (kadar
maksimum)
Application
Dairy industry
Industri susu
Enzyme
Rennin
Renin
Use
Coagulant for milk and in
cheese production
Pengumpalan protein susu dan
penghasilkan keju
Lactase
Laktase
Brewing industry
Industri
penampaian
simpanan
bir
Biological
detergents
Detergen biologi
Protease,
lipase
and amylase
Protease, lipase
dan amilase
Baking industry
Industri roti
Amylase
Amilase
Menyingkirkan
tanda-tanda
organik seperti darah, air liur,
minyak.
Protease
Breakdown of proteins
Menguraikan protein
Meat industry
Industri daging
Textile industry
Industri tekstil
Glucose oxidase
Glukosa oksidase
Protease, papain
Tenderisation of meat
Melembutkan daging
Amylase
Amilase
Leather industry
Protease
Industri kulit
Paper industry
Industri kertas
Ligninase
6. Recently, cellulase has been added to brighten the colors and soften
the cloth.
Selulase ditambah ke dalam detergen untuk menjadikan warna pakaian lebih
terang dan melembutan pakaian.
Glucose
Glukosa
(Akibat kekurangan)
Starch
Kanji
Glycogen
Glikogen
Cellulose
Selulosa
Major
carbohydrate Liver and muscle cells
storage in animal cells
would not be able to carry
out their high metabolic
Simpanan
karbohidrat activities.
utama dalam sel haiwan
Microtubules
Mikrotubulus
Sel
tumbuhan
kehilangan
bentuk. dan pecah apabila air
yang berlebihan memasuki sel
unable to
Organisma
unisel
seperti
Paramesium dan Euglena gagal
bergerak.
Haemoglobin
Hemoglobin
Carrier of oxygen
Mengangkut oksigen
Sel
darah
merah
mengangkut oksigen
proses kehidupan.
Major
carbohydrate The cells lack energy to
storage in plant cells
grow and divide, such as
during seed germination.
Simpanan
karbohidrat
utama dalam sel tumbuhan
secara osmosis.
Microfilaments
Mikrofilamen
Antibodies
Antibodi
Hormones
Hormon
Lemak dan
minyak
Waxes
Lilin
gagal
Certain
unicellular Unicellular
aquatic
aquatic
organisms organism unable to float
produce oil droplets to
Organisma unisel akuatik tidak
aid buoyancy.
Membantu
organisma
akuatik untuk terapung
Waterproofing material
Sel
tumbuhan
mengalami
kehilangan air yang berlebihan
Phospholipids
Fosfolipid
Steroids
Steroid
Enzyme
Enzim
Cholesterol strengthens
the cell membranes,
manufactures vitamin D
and sex hormones.
Kolesterol memperkuatkan
membran sel, menghasilkan
vitamin D dan hormon
pembiakan.
(b) The figure shows a reaction of an enzyme and its substrate. Explain
the mechanism of the enzymatic reaction.
[10 marks]
Rajah menunjukkan enzim dan substrat. Terangkan mekanisme tindak balas enzim.
Question 1:
The figure shows the organelles involved in the production of extracellular
enzymes.
Rajah menunjukkan organel-organel yang terlibat dalam penghasilan enzim luar sel.
(a) (i) Using a name example, explain the term extracellular enzyme.
Dengan menggunakan satu contoh, terangkan istilah enzim luar sel.
[2 marks]
__________________________________________________________
(ii) Based on the organelles shown, explain how extracellular enzymes
are produced.
Bedasarkan organel-organel yang ditunjukkan, terangkan bagaimana enzim luar sel
dihasilkan.
[8 marks]
Question 2:
Discuss the uses of enzymes in the household & industries.
Why are enzyme used in these industries.
Bincangkan kegunaan enzim dalam rumah tangga dan industri.
Mengapakah enzim tersebut digunakan dalam industri tersebut.