Documente Academic
Documente Profesional
Documente Cultură
Uses of
Synthetic Biology
for Renewable
Chemicals,
Pharmaceuticals,
and Biofuels
M.PeteHe,PhD.,Senior
ResearchFellow,Corporate
Sustainability,Dial,Henkelof
Americasays:Sustainable
chemistrywillbeamajor
driverofU.S.economic
growth,andIlookforwardto
moreofthistypeof
impressivework,whichisa
winwin,combiningacademic
achievementwithindustrial
implications.
andotherformulations.Thehullisthewoodycasethatprotectsthesoybeans,anditcannotbedigested
byhumansorothermonogastricanimals,suchaspigs.TheU.S.
producesabout70billionpoundsofindigestiblesoy
carbohydrateannually,andModularseekstoupgradethis
underutilizedmaterialbyconvertingitintoavarietyofuseful
newchemicalproducts.Modularssurfactantprogramispartially
supportedwithfundsfromtheNewUsesCommitteeofthe
UnitedSoybeanBoard(USB),whichseekstoexpandsoybean
marketsthroughthedevelopmentoftechnologythatenablesthe
conversionofsoybasedmaterialsintonewproducts.
Today,mostorganicchemicalsarederivedfrompetroleum.
FredrickFrank,ViceChairman,PeterJ.SolomonCompany,offersthisperspectiveonthesustainable
chemistryindustry:Severalpublishedreportshaveconcludedthatabouttwothirdsofthosechemicals
canbegeneratedfromrenewablerawmaterials,ratherthanfromoil.Ifso,sustainablechemistry
potentiallyhasamarketsizeofabout$1trillion.Lessthan7percentoforganicchemicalsarecurrently
producedfromrenewablematerials,thusthereisanopportunityforlongtermgrowth.
trialsareongoing.In2009,GeneArtwasawardedacontractbytheHIVVaccineConsortium(UK)to
designandproducetwoHIVvaccinecandidatesbasedontheHIVgenesequencesusedinthe2008trial.
InMay2009,theGeneArtgenesynthesisandassemblyplatformwasemployedtocreatesynthetic
H1N1genes,andtheproductwasdeliveredwithina5dayperiod.GeneArtcreatedanadditionalten
H1N1viralcoatproteinconstructsfortheRobertKochInstitute(thecentralfederalinstitution
responsiblefordiseasecontrolandpreventioninGermany).
Verdezyneisdevelopingacostadvantaged,environmentallyfriendlyfermentationprocessforadipic
acid.Thecompanysproprietarymetabolicpathwaycanutilizesugar,plantbasedoilsoralkanes,and
thecompanyhascompletedproofofconcepttestingforfattyacidsand
alkanes.Thepotentialbenefitofthisfeedstockflexibleapproachistheability
tomaintainasustainableeconomicadvantageregardlessoffutureenergy
volatilityandtoreducetheenvironmentalfootprintforproducingadipic
acid.
Adipicacidisnotproducedinnature.Verdezynesnovelcombinatorial
approachtopathwayengineeringrapidlycreatesandharnessesgenetic
diversitytooptimizeametabolicpathway.Ratherthanmanipulatingone
pathwaygeneatatime,thecompanyusessyntheticgenelibrariesto
introducediversityintoametabolicpathway.Thecompanysuniquecomputationalandsynthetic
biologytoolboxallowseffectivedesign,synthesisandexpressionofsynthesizedgenesinaheterologous
recombinantyeastmicroorganism.
improveyield,productivity,andtiterthedriversofprocesseconomicefficiencythebiosynthetic
pathwaysandhostmetabolismhaverequiredsignificantgeneticoptimization.LS9developed
capabilitiesforthecomputationaldesignandautomatedparallelconstructionofgene,operon,and
recombinantcelllibrariesthathaveenabledtherapidconstructionandevaluationofthousandsof
rationallyengineeredmicroorganisms.Thiscapabilityincombinationwithstateoftheartscreening,
processdevelopment,andanalyticalmethodologieshasenabledLS9inonlyafewyearstoadvance
fromconcepttoaprocessslatedforcommercialscaledemonstration.
Thissametechnologyplatformhasbeenleveragedfortheproductionofsurfactantsforuseinconsumer
productsincollaborationwithProcter&Gamble.Theabilitytoexchangebiosyntheticpartsand
leveragethecorehostchassishasenabledthedevelopmentofthischemicalproductlinemuchfaster,
achievinginmonthswhathadtakenyearsfortheearlierproducts.
LS9intendstocontinuetoleveragethepowerofsyntheticbiologytofurtheradvancetheseandfuture
productsasquicklyandcosteffectivelyaspossible.Wefeelstronglythesetechnologiesareessentialto
thegoalofweaningourdependenceonfossilfeedstocksandthefurtherdevelopmentofaworld
leadingindustrialbiotechnologyindustry.
ThishasallowedMetabolixtosuccessfullycommercializeMirelbioplastics.Morethan50yearsafterit
wasfirstconsideredasapotentiallyusefulnewmaterialandfollowingseveraleffortsbyleading
chemicalcompaniestocommercializePHAsbasedonnaturalproductionhosts,Metabolixhasmade
theseproductsavailableatacommercialscale.
Intheconventionalpetrochemicalprocess,benzeneor
butaneisoxidizedtoproducemaleicanhydridereleasing
roughlyhalfoftherawmaterialasCO2whichisthen
convertedtosuccinicacidthroughhydrolysis.
Myrianthasdevelopedamoreecofriendlymethodforthe
productionofbiosuccinicacidthataddressesthesupply,
costandenvironmentalissuesassociatedwiththeuseof
fossilfuelsasfeedstocks.Myriantstechnologyplatformuses
proprietarymicroorganismsinafermentationprocess
designedtoproducehighvaluebiobasedchemicalswith
highyieldsandproductivity.Thebiocatalystsarenon
geneticallymodified(nonGMO)microorganismswithaltered
metabolicpathwaysthatgrowandsimultaneouslyproduce
targetbiobasedchemicalsfromavarietyofrenewable
feedstocks.Ourhighlyefficientbiocatalystsareableto
flourishinminimalgrowthmediumwithouttheuseof
expensive,complexnutrients,enablingustobringrenewable
chemicalstomarketthatarecostcompetitivewiththeir
petroleumbasedcounterparts.
Myriantsbiocatalystsareabletoconsumeavarietyofreadilyavailablelowcostsugarsfromnonfood
sources,suchasglucosefromsorghumandcellulosicsugarsfromwastebiomass.Theserawmaterials
arenotsubjecttotheextremepricevolatilityorsupplyavailabilityissuesassociatedwithpetroleum
basedfeedstocks.Additionally,MyriantsanaerobicprocessconsumesCO2,resultinginareduced
carbonfootprint.
Biosuccinicacidcanbeusedinmanyapplications,suchasplasticizers,coatings,polymers,esters,and
polyesterpolyols.Asanexample,MyrianthasdevelopedMyrifilmsolvent,whichcanbeusedto
manufacturecoatingswithsuperiorfunctionalperformance.Myrifilmalsobringsadditionalbenefitsto
thecoatingsformulator,asitcontainsnovolatileorganiccompounds(VOC),isfreeofhazardousair
pollutants(HAPS),andisodorfree.
In2009,Myriantreceiveda$50milliongrantfromtheU.S.DepartmentofEnergytobuildthenations
firstbiosuccinicacidcommercialproductionfacilityinLakeProvidence,La.Theplantisscheduledto
startupinthefirstquarterof2013andits30millionpoundperyearproductioncapacityisalreadysold
tocustomersaroundtheworld.