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May | June 2010 Feature title: LiptoCitro - Phytobiotics and Prebiotics: a new alternative for
May | June 2010 Feature title: LiptoCitro - Phytobiotics and Prebiotics: a new alternative for
May | June 2010 Feature title: LiptoCitro - Phytobiotics and Prebiotics: a new alternative for

May | June 2010

Feature title: LiptoCitro - Phytobiotics and Prebiotics: a new alternative for sustainable aquaculturePhytobiotics and Prebiotics

International Aquafeed is published five times a year by Perendale Publishers Ltd of the United Kingdom. All data is published in good faith, based on information received, and while every care is taken to prevent inaccuracies, the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis of information published. ©Copyright 2009 Perendale Publishers Ltd.All rights reserved. No part of this publication may be reproduced in any form or by any means without prior permission of the copyright owner. Printed by Perendale Publishers Ltd. ISSN: 1464-0058

F: LiptoCitro

F: LiptoCitro Phytobiotics and Prebiotics A new alternative for sustainable aquaculture by Santiago de la Cuesta,

Phytobiotics and Prebiotics

A new alternative for sustainable aquaculture

by Santiago de la Cuesta, Ignacio López, Antonio Martínez and Laura Muñoz, Spain Email: liptosa@liptosa.com, Website: www.liptosa.com

E nvironmentally friendly LiptoCitro is a prebi- otic, immunoestimulant and growth promoter with a

proved antibacterial activity. It can be used at any stage of the fish produc- tion cycle and no withdrawal period is needed because it does not contain any antibiotics. It is used at dosage rates of 2.5Kg/M/tonne and it can be incorporated in the feed mill during diet manufacture, or top-dressed on the feed using oil or molasses at the farm.

It is made of a combination of prebiotics, plant extracts and plant essences and does not develop off-flavours in either fish or crustaceans. LiptoCitro is capable of improv- ing nutrient digestibility, favouring beneficial intestinal flora, eliminating pathogenic bacteria, and promoting the immune system, resulting in higher resistance to stress and disease and improved growth, survival, FCR, production and cost-efficiency, even in the event of bacte- rial, viral and some parasitic disease outbreaks.

LiptoCitro trial data

Trials have been conducted in laborato- ries, research centres and farms

with a range of fish and shrimp species and pathogens. Trials conducted in the Veterinary Sanitary Vigilance Centre (VISAVET) of the Complutense University of Madrid, Spain showed that LiptoCitro possessed strong in vitro antibacterial activity against a range of pathogenic bacterial species commonly encountered in aquaculture production systems (Table 1). Trials carried from May to November in a commer- cial farm in Spain showed the efficacy of Liptocitro to con- trol Haemorrhagic Enteritis outbreaks in rainbow trout (Oncorhynchus mykiss). A total amount of 1,700 MT of commer- cial feed with LiptoCitro added at 2.5Kg/MT was used. All the fish had the same handling and usual European vaccine prophylaxis. In the control lots, which were not fed Liptocitro, several Enteritis outbreaks occurred over the six month trial peri- od and the fish had to be treated with antibiotics. However,

the lots of infected fish that were fed with LiptoCitro improved significantly,

Table 1: Minimum Inhibitory Concentration (MIC) of LiptoCitro against bacterial strains tested in VISAVET laboratory, Spain

Microorganism

MIC

Aeromonas hydrophila

3

mg/ml

Aeromonas salmonicida subsp. salmonicida

1

mg/ml

Listonella anguillarum

6

mg/ml

Listonella anguillarum CECT 522

3

mg/ml

Leucothrix mucor DSM 2157T

1

mg/ml

Vibrio penaeicida DSM 14398T

3

mg/ml

Vibrio harveyi CECT 525T

1

mg/ml

Vibrio parahaemolyticus

6

mg/ml

Vibrio ordali

6

mg/ml

Vibrio vulnificus

1

mg/ml

Vibrio vulnificus CECT 529

0.1

mg/ml

Vibrio alginolyticus

6

mg/ml

Flavobacterium psycrophilum DSMZ 3660T

3

mg/ml

Edwarsiella ictaluri CECT 885T

13

mg/ml

Brevundimonas diminuta CECT 317T

1

mg/ml

Pseudomonas anguilliseptica

0.1

mg/ml

Pseudomonas fluorescens

3

mg/ml

Yersinia ruckeri

3

mg/ml

Photobacterium damselae subsp. piscicida

3

mg/ml

Photobacterium damselae subsp. damselae

6

mg/ml

Lactococcus garvieae

6

mg/ml

Streptococcus iniae CCUG 27303T

13

mg/ml

Streptococcus difficilis DSZM 16828

13

mg/ml

Streptococcus parauberis

6

mg/ml

Mycobacterium chelonae CCUG 47445T

13

mg/ml

Mycobacterium marinum

0.1

mg/ml

7,97%
7,97%

24 | InternatIonal AquAFeed | May-June 2010

F: LiptoCitro

Table 2: Effect of prebiotic LiptoCitro on Daily Intake Rate (DIR), Feed Conversion Ratio (FCR) and Survival Rate in sea bass (Dicentrarchus labrax). Data from six month laboratory trial using four replicates (eight tanks total) with 100 fish per tank. *Dosages of 3g/Kg. Values shown are the mean ± standard error

 

Feed

 

% Effect

 

Without

Feed With

(LiptoCitro

Significance

LiptoCitro

LiptoCitro*

vs.

(Control)

Control)

 

DIR

1.28

± 0.04

1.19

± 0.04

7.03%

p<0.05

FCR

1.38

± 0.05

1.27

± 0.04

7.97%

p<0.05

Survival Rate

94.75 ± 6.13

98.00 ± 1.41

3.25%

n.s.

recovered and did not need any antibiotic treatment at any stage of the trials. Trials conducted throughout a production

increasing the inclusion of cheaper vegetable

ment of the growth and survival rates. LiptoCitro anti-

cycle on rainbow trout (Oncorhynchus mykiss), under commercial conditions and using con- trol lots, showed 14 percent improvement in FCR in the fish that were fed with 2.5 Kg/MT of LiptoCitro. This digestibility improvement was evaluated in further trials using the same inclusion rates of Liptocitro in the feed. The results showed that the cost of the ration could be reduced without altering FCR. This was achieved by decreasing the amount of expensive animal origin protein in the diet and

bacterial efficiency, survival and FCR improvement and growth and immune system promoter effect have also been tested in vari- ous shrimp species and under different farming and envi- ronmental condi- tions. These results

protein instead. Six month trials were conducted in four replicate tanks in the laboratories of the Agricultural Development and Food Research

can be provided upon request. Trials carried out with LiptoCitro

Institute IMIDA, in Murcia, Spain. These

have shown that the

trials were carried on European sea bass

use of prebiotics

(Dicentrarchus labrax) under ideal conditions

and phytobiotics in

and without a disease challenge. The fish fed three g/kg of Liptocitro showed three percent improvement in growth and survival

fish and shrimp feed leads into better production param-

rates and eight percent improvement in FCR over the fish in the control tanks (statisti-

eters and enables sustainable aquacul-

cally significant at 95 percent confidence level)

ture production on

(Table 2). Improvements in the haemocrit and haemoglobin levels of fish blood following a stress
(Table 2). Improvements in the haemocrit and
haemoglobin levels of fish blood following a
stress test were also observed.
a
long term basis
by
reducing the use
These trials have shown how a better
intestinal integrity, even under ideal conditions
of low density and very low mortality, results
in better nutrient assimilation thus improving
the feed conversion rate. Therefore, in the
field, a better nutrient
of antibiotics and
decreasing fish meal
content in diets.
R e f e r e n c e s
are available upon
request.
assimilation as a result
of a good intestinal
More infoirMation:
integrity will have
even more advanta-
geous effects than
those observed under
ideal conditions with
regards the improve-
Antonio Martinez
Lipidos Toledo SA, Spain
Tel: +34 902157711Fax: +34 913567300
Email: antonio.m.sanchez@liptosa.com
Web: www.liptosa.com

May-June 2010 | InternatIonal AquAFeed | 25

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VOLUME 13 ISSUE 3 2010 Alternative Lipid Sources in Aquafeeds Phytobiotics and Prebiotics: - a
VOLUME 13 ISSUE 3 2010
Alternative Lipid Sources
in Aquafeeds
Phytobiotics and
Prebiotics:
- a new alternative for sustainable
aquaculture
Innovative approaches
to reduce feed cost in
aquaculture:
- Optimizing nutrient utilization and gut health
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