Documente Academic
Documente Profesional
Documente Cultură
Blood
Semen
Skin cells
Tissue
Organ
Perspiration
Fingernails
Mucus
Saliva
Muscles
Brain
Bone
Etc
Body fluids
- blood
- semen
- saliva
- sweat
- urine
Hair
Teeth
Bones
Facts on DNA
The same DNA is found in all cells of the body. The
same DNA profile is recovered, no matter what the
sample (cell) type.
DNA is unique for all individuals except identical
twins.
Your DNA does not change through your life.
Any cellular material left at a crime scene may be a
useful source of DNA.
DNA is formed by combination of DNA from his or her
parents with half coming from the mother and half
from the father. is unique for all individuals except
idencal twins.
Baseball bat or
similar weapon
Handle, end
Hat, bandanna,
mask
Inside
Facial tissue,
cotton swab
Surface area
Dirty laundry
Surface area
toothpick
Surface area
Saliva, blood
Fingernail, partial
fingernail
scrapings
Through and
through bullet
Outside surface
Blood, tissue
eyeglasses
Sweat, skin
Used cigarette
Cigarette butt
Saliva
Stamp or envelop
Licked area
Saliva
Tape or ligature
Skin, sweat
Bottle, can, or
glasses
Side, mouthpiece
Saliva, sweat,
Bite mark
saliva
Used condom
Inside/outside surface
Blanket, pillow,
sheet
Surface area
tissue
cigarette butt
fingernails
Broken window
Collection Priority
The first Priority are trace materials and
evidence of a fragile nature.
The second priority would be to collect
swabs from handled items that have been
moved, are out of place or do not belong to
the resident.
A third priority type of evidence that may
be at the scene includes the potentially
lower-quality biological evidence.
GENERAL GUIDELINES
Degradation of biological evidence is
most commonly caused by exposure to
moisture and heat, which encourages the
growth of bacteria.
This can lead to destruction of the
DNA and the possibility of incomplete or no
DNA typing results being obtained.
Collection Procedures
Personal Protection
BIOLOGICAL MATERIALS CAN CONTAIN PATHOGENS SUCH AS:
Hepatitis
Syphilis
TB
Gonorrhea
Measles
HIV
NOTE: Assume that all stains, wet or dry, are infectious!
Special Situations
For Liquid Containers:
Empty liquid containers by poking a hole in the
bottom to avoid liquid contact with the mouth area.
Swab mouth area of the container using sterile
cotton swabs. Package in paper envelope.
Submit entire item if swabbing is not possible.
Request test for both DNA and latent fingerprints.
Mark aluminum cans for "Room Temperature
Storage," as cold or frozen storage causes
condensation on metal, which may dilute biological
evidence.
Scraping method.
Use a new or clean scalpel blade to scrape the stains from the surface.
Collect the flakes onto clean paper and fold the paper in a bindle.
If the stain is on wood, shave the area of the bloodstain with a new or clean
scalpel blade.
Cigarette Butts
Select only the cigarette butts that may be of
evidentiary value.
Collect individually
Air dry before packaging in PAPER envelope
Do NOT handle with your bare fingers.
Do NOT include the ashes.
Fingernail Swabs
Fingernail Scrapings
Fingernail Swabs:
Moisten a sterile cotton swab with distilled water
and swab under fingernails.
Use one swab per hand.
Place the evidence in separate labeled envelopes.
Fingernail Scrapings:
Collect scrapings using a rounded end of a toothpick.
One toothpick per hand.
Collect the scrapings (and the toothpick) into
druggist fold (use a separate piece for each hand).
Place the evidence in separate labeled envelopes
Hair
Collect all questioned hairs from the scene that
have possible evidentiary value.
Note position of hairs in the crime scene.
Use clean forceps or gloves to collect.
Place in druggist folds; try not to bend samples.
Avoid damaging the root of the hair.
Air dry if wet, prior to packaging.
Combing: Using clean exam paper and comb
thoroughly and vigorously comb questioned
region (head/public) and collect all hairs
recovered.
Deceased Subjects
1. Buccal Swabs: without blood in the oral cavity.
2. Blood swab: Liquid blood sample removed from body at
time of autopsy must be stained on a sterile cotton swab and
air dry.
3. Best tissue sample: If no blood sample is available, collect
an approximate inch square piece of the best tissue sample
available, (psoas muscle, liver, spleen, stomach).
4. Bone marrow or teeth with tissue: If no tissue sample is
available.
5. If cadaver is embalmed: cut skin from earlobe, prevent
cutting too deep as formalin may leak.
Optional: Hair sample may be collected as additional
standard sample. Collect at least 15 head or pubic hairs
(PULLED, NOT CUT), package, label and submit to
Secondary Standard
Secondary standards are collected when collection of
primary standards is impossible, such as in cases of missing
victims.
Buccal swabs from parents, or
Buccal swabs from child and wife, or
Buccal swabs from a known identical twin/
Personal Belongings such as toothbrush, razor,
Rape
From Victim:
Stained underwear or
clothing
Vaginal Swabs
Fingernail Clippings
Standard
From Suspect
Clothing with bloodstain
Standard
From Crime Scene
Seminal stains
Bloodstains
Stabbing Incident
From Victim:
Fingernail Clippings
Standard
From Suspect
Clothing with bloodstain
Standard
Weapon
From Crime Scene
Bloodstains
Shooting Incident
From Victim:
Fingernail Clippings
Standard
From Suspect
Clothing with bloodstain
Standard
Weapon
From Crime Scene
Bloodstains
CHAIN OF CUSTODY
Record of individuals who have had
physical possession of the evidence.
Integrity of chain of custody
The fewer people handling the evidence,
the better, the lesser chance of
contamination and a shorter chain of
custody for court admissibility hearings.
SEAL
MARKINGS on Evidence
Information to ensure that the items can be
identified by the collect anytime in the
future.
This precaution will help immeasurably to
establish the credibility of the collectors
report or testimony and will effectively avoid
any suggestions that the item has been
misidentified.
Markings on Evidence
A. Case number.
Markings on Evidence
DATE AND TIME OF COLLECTION
08-12-2012
0600H
STAIN
CASE NUMBER
IDENTIFIER
Integrity seal
Initial/Signature
of the Collecting
Officer/who did
the packaging
SPECIMEN
DESCRIPTION
LOCATION WHERE
SPECIMEN WAS FOUND
IDENTIFIER
1.Letter Request
2.Court Order
3.Chain of custody form (Ideal)
LIMITATIONS
DNA cannot be used to:
Tell how long the suspect was at the crime
scene.
Tell how long the suspect handled an object.
Determine how long ago the cells were
deposited on the item.
Common requests:
Latent Prints
Firearms
Important to decide based on case,
which evidence has priority
LIMITATIONS
Environmental factors such as heat,
bacteria and mold can destroy DNA
evidence.
Identical twins share identical DNA.
DNA can not be used to determine WHEN
the suspect was at the crime scene.
CASE STUDY
Case no.1: Paternity
Php 200,000,000.00+++
Answer: Results showed that the DNA profile found on his shirt
and shoes matched the DNA profile of the lady companion of the
woman.