Documente Academic
Documente Profesional
Documente Cultură
Equipment
Forceps
Alcohol in beaker
Alcohol lamp
Matches
Permanent marker (Sharpie fine point)
Disinfectant in squirt bottle
Incubator at 37 C (optional)
Safety glasses
Latex or Nitrile exam gloves
Roll of transparent tape (e.g. Scotch tape)
Laboratory Journal
Procedure
1. Put on safety glasses and exam gloves.
2. Spray disinfectant on the area you will be working, and wipe it clean.
3. Get one Petri dish containing Bacillus cereus and another containing Escherichia
coli. These dishes contain live bacterial cultures. You cannot see the bacteria
growing because the cultures are less than two hours old and the bacteria have
not multiplied sufficiently to be visible.
4. Write your initials or first names on the outside edge of each Petri dish. Dont
write too large or your writing will interfere with your observation of the results.
5. Place one Petri dish over the diagram below.
6. Use the technique demonstrated by your teacher to sterilize the forceps with
flaming alcohol.
7. Use the sterile forceps to place the control disk in the center position on the Petri
dish.
8. Expel one disk of each of the six types of antibiotic from its vial onto the agar in
the Petri dish. Use the circles on the diagram to position the disks.
9. Repeat steps 5 to 8 with the second Petri dish culture.
10. Seal the Petri dishes using transparent tape.
11. Spray disinfectant on the work area and wipe it clean.
12. Remove your gloves and dispose of them as directed by your teacher.
13. Wash your hands and forearms thoroughly with soap and warm water.
14. Make a copy of the diagram in your laboratory notebook for each culture and
record which disk you placed at each position.
15. Make a data table in your Laboratory Journal similar to one shown below with
enough rows for each of the antibiotics.
DISK
Antibiotic
Color-Code
E. coli
48 Hours
B. cereus
E. coli
Conclusion
1 Why is the clear area surrounding an antibiotic disk called the zone of inhibition?
24. Which antibiotic would be the most effective treatment for an infection with the E.
coli? Explain your response.
25. Which antibiotic would be the most effective treatment for an infection with the B.
cereus? Explain your response.
26. Which antibiotic would be the most effective treatment for a person with an
infection with both B. cereus and E. coli? Explain your response.
27. How were the results after 48 hours different from the results after 24 hours?
29. Propose reasons why an antibiotic may not be as effective at inhibiting E. coli as
it is against B. cereus.