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DOG ROSE
Rosae pseudo-fructus
DEFINITION
Rose hips made up by the receptacle and the remains of the
dried sepals of Rosa canina L., R. pendulina L. and other Rosa
species, with the achenes removed.
Content : minimum 0.3 per cent of ascorbic acid (C6H8O6 ;
Mr 176.1) (dried drug).
IDENTIFICATION
A. It consists of fragments of the eshy, hollow, urceolate
receptacle, bearing the remains of the reduced sepals, light
pink or orange-pink, the convex outer surface shiny and
strongly wrinkled ; bearing on its lighter inner surface
abundant bristle-like hairs.
B. Reduce to a powder (355) (2.9.12). The powder is
orange-yellow. Examine under a microscope using chloral
hydrate solution R. The powder shows the following
TESTS
diagnostic characters : numerous fragments of receptacle,
the outer epidermis with orange-yellow contents and a
Digitalis lanata Ehrh. The presence of leaves with few or no
thick cuticle, the inner epidermis composed of thin-walled
trichomes and with parallel venation or the presence of cells
cells containing cluster crystals and occasional prisms of
of the abaxial epidermis with beaded anticlinal walls and of
calcium oxalate ; scattered lignied cells, isodiametric,
cells of the adaxial epidermis with numerous stomata indicates
with thickened and pitted walls forming the trichome
adulteration by Digitalis lanata Ehrh.
bases ; abundant unicellar trichomes, up to 2 mm long and
Loss on drying (2.2.32): maximum 6.0 per cent, determined
30-45 m thick, tapering towards each end, walls heavily
on 1.000 g of the powdered herbal drug (355) (2.9.12) by
thickened and with a waxy cuticle which may show ssures
drying in an oven at 105 C.
in a spiral arrangement ; numerous oily orange-yellow
globules.
Total ash (2.4.16) : maximum 12.0 per cent.
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Drynaria rhizome
A1
A2
m1
m2
07/2012:2563
DRYNARIA RHIZOME
Drynariae rhizoma
DEFINITION
Dried rhizome of Drynaria fortunei (Kunze) J. Sm. The
ramenta may be removed.
Content : minimum 0.5 per cent of naringin (C27H32O14 ;
Mr 580.5) (dried drug).
IDENTIFICATION
A. Long, attened, slat-shaped rhizome, often curved and
TESTS
branched, 5-15 cm long and 1-1.5 cm thick. The surface
Foreign matter (2.8.2) : maximum 1 per cent.
is either completely covered in scaly, dark brown hairs
(rhizome with ramenta) or glabrous with dark brown dots
Loss on drying (2.2.32) : maximum 10.0 per cent, determined
(rhizome without ramenta). The upper surface and both
on 1.000 g of the powdered herbal drug (355) (2.9.12) by
sides show circular frond scars, rarely the frond bases. The
drying in an oven at 105 C.
lower surface shows scars or the remains of brous roots.
Total ash (2.4.16) : maximum 7.0 per cent.
The texture is light, fragile, easily broken. The section is
reddish-brown ; the steles form a ring of small yellow dots.
ASSAY
B. Microscopic examination (2.8.23). The powder is
Test solution. In a round-bottomed ask, weigh 0.500 g
reddish-brown. Examine under a microscope using chloral
of the freshly powdered herbal drug (710) (2.9.12). Add a
hydrate solution R. The powder shows the following
solution of 1.0 g of oxalic acid R in 50.0 mL of methanol R.
diagnostic characters : numerous parenchyma fragments,
Boil under a reux condenser for 10 min, and cool in
consisting of polyhedral cells with slightly and regularly
iced water until the temperature reaches 15-20 C. Filter.
thickened and pitted walls ; scalariform lignied vessels
Transfer 2.0 mL of the ltrate to a 50 mL conical ask.
of variable diameter up to 60 m ; fragments of scaly
Add successively, with gentle shaking after each addition,
hairs forming a tissue consisting of many reddish-brown
2.0 mL of dichlorophenolindophenol standard solution R
cells forming expansions on the margins (rhizome with
and then, exactly 60 s later, 0.5 mL of a 100 g/L solution
ramenta).
of thiourea R in ethanol (50 per cent V/V) R and 0.7 mL of
dinitrophenylhydrazine-sulfuric acid solution R. Heat under a C. Thin-layer chromatography (2.2.27).
reux condenser at 50 C for 75 min, and place immediately
Test solution. To 0.5 g of the powdered herbal drug (355)
in iced water for 5 min. Add dropwise 5.0 mL of a mixture of
(2.9.12) add 5 mL of methanol R and sonicate for 10 min.
12 mL of water R and 50 mL of sulfuric acid R, taking care to
Cool, centrifuge and use the supernatant.
carry out the addition over a period of minimum 90 s and
Reference solution. Dissolve 1 mg of naringin R and 1 mg
maximum 120 s while maintaining vigorous stirring in iced
of hyperoside R in 2 mL of methanol R.
water. Allow to stand for 30 min at room temperature and
Plate : TLC silica gel plate R (2-10 m).
measure the absorbance (2.2.25) at 520 nm using solution A
as compensation liquid.
Mobile phase : acetic acid R, anhydrous formic acid R,
water R, ethyl acetate R (11:11:26:100 V/V/V/V).
Solution A. Treat 2.0 mL of the ltrate obtained during the
preparation of the test solution as described but adding the
Application : 10 L as bands of 8 mm.
dinitrophenylhydrazine-sulfuric acid solution R just before the
Development : over a path of 6 cm.
absorbance is measured.
Drying : in air.
Reference solution. Dissolve 40.0 mg of ascorbic acid R in a
Detection : treat with aluminium chloride reagent R ;
freshly prepared 20 g/L solution of oxalic acid R in methanol R
examine in ultraviolet light at 365 nm.
and dilute to 100.0 mL with the same solvent. Dilute 5.0 mL
of this solution to 100.0 mL with a freshly prepared 20 g/L
Results : see below the sequence of zones present in the
solution of oxalic acid R in methanol R. Treat 2.0 mL of the
chromatograms obtained with the reference solution and
solution as described above for the ltrate obtained during
the test solution. Furthermore, other faint zones may
the preparation of the test solution. Measure the absorbance
be present in the chromatogram obtained with the test
(2.2.25) at 520 nm using solution B as the compensation liquid.
solution.
General Notices (1) apply to all monographs and other texts
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