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How many bands will appear on the gel in the insulin sample?
One as well
Question 7
Explain why mass spectrometry, which is highly accurate, cannot distinguish Leu and Ile.
Mass spectrometry cannot distinguish because the two are chemically identical
Question 9
Myoglobin transfers oxygen obtained from hemoglobin to mitochondrial proteins involved in the catabolism
of metabolic fuels to produce energy for the muscle cell. Using what you have learned in this chapter about
myoglobin and hemoglobin, what can you conclude about the structures of these mitochondrial proteins?
These mitochondrial proteins probably have the oxygen receiving part of the protein trapped in the
center so water cant readily react with it. Perhaps they have heme groups.
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Lecture 6
Question 2-3
Hemoglobin is 50% saturated with oxygen when pO2 = 26 torr. If hemoglobin exhibited hyperbolic binding
(as myoglobin does) with 50% saturation at 26 torr, what would be the fractional saturation when pO2 = 30
torr? What would be the fractional saturation when pO2 = 100 torr? What does this tell you about the
physiological importance of hemoglobins sigmoidal oxygen-binding curve?
Question 7
Phalloidin, a peptide isolated from a poisonous mushroom, binds to F-actin but not G-actin. How does the
addition of phalloidin affect cell motility?
It could prevent polymerization of the actin strand
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