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TissuecultureWikipedia,thefreeencyclopedia

Tissueculture
FromWikipedia,thefreeencyclopedia

Tissuecultureisthegrowthoftissuesorcellsseparatefromtheorganism.
Thisistypicallyfacilitatedviauseofaliquid,semisolid,orsolidgrowth
medium,suchasbrothoragar.Tissueculturecommonlyreferstothe
cultureofanimalcellsandtissues,withthemorespecifictermplanttissue
culturebeingusedforplants.Theterm"tissueculture"wascoinedby
AmericanpathologistMontroseThomasBurrows,M.D.[1]

Contents
1
2
3
4
5

Historicalusage
Modernusage
Seealso
References
Externallinks

Historicalusage

Flaskscontainingtissueculture
growthmediumwhichprovides
nourishmenttogrowingcells.

In1885WilhelmRouxremovedasectionofthemedullaryplateofan
embryonicchickenandmaintaineditinawarmsalinesolutionforseveral
days,establishingthebasicprincipleoftissueculture.In1907thezoologistRossGranvilleHarrisondemonstrated
thegrowthoffrogembryoniccellsthatwouldgiverisetonervecellsinamediumofclottedlymph.In1913,E.
Steinhardt,C.Israeli,andR.A.Lambertgrewvacciniavirusinfragmentsofguineapigcornealtissue.[2]In1996,
thefirstuseofregenerativetissuewasusedtoreplaceasmalllengthofurethra,whichledtotheunderstandingthat
thetechniqueofobtainingsamplesoftissue,growingitoutsidethebodywithoutascaffold,andreapplyingit,can
beusedforonlysmalldistancesoflessthan1cm.[3]
GottliebHaberlandtfirstpointedoutthepossibilitiesofthecultureofisolatedtissues,planttissueculture.[4]He
suggestedthatthepotentialitiesofindividualcellsviatissuecultureaswellasthatthereciprocalinfluencesof
tissuesononeanothercouldbedeterminedbythismethod.SinceHaberlandt'soriginalassertions,methodsfor
tissueandcellculturehavebeenrealized,leadingtosignificantdiscoveriesinbiologyandmedicine.Hisoriginal
idea,presentedin1902,wascalledtotipotentiality:Theoreticallyallplantcellsareabletogiverisetoacomplete
plant.[5][6][7]

Modernusage
Inmodernusage,tissueculturegenerallyreferstothegrowthofcellsfromatissuefromamulticellularorganism
invitro.Thesecellsmaybecellsisolatedfromadonororganism,"primarycells",oranimmortalisedcellline.The
cellsarebathedinaculturemedium,whichcontainsessentialnutrientsandenergysourcesnecessaryforthecells'
survival.[8]Thetermtissuecultureisoftenusedinterchangeablywithcellculture
Theliteralmeaningoftissueculturereferstotheculturingoftissuepieces,i.e.explantculture.

https://en.wikipedia.org/wiki/Tissue_culture

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TissuecultureWikipedia,thefreeencyclopedia

Tissuecultureisanimportanttoolforthestudyofthebiologyofcellsfrom
multicellularorganisms.Itprovidesaninvitromodelofthetissueinawell
definedenvironmentwhichcanbeeasilymanipulatedandanalysed.
Planttissuecultureinparticularisconcernedwiththegrowingofentire
plantsfromsmallpiecesofplanttissue,culturedinmedium[9]

Seealso
Cellculture
Organculture
Planttissueculture

Culturedcellsgrowingingrowth
medium

References
1.Carrel,AlexisandMontroseT.BurrowsCultivationofTissuesinVitroanditsTechniqueJournalofExperimental
Medicine13(1911:38796)
2.Steinhardt,EIsraeli,CandLambert,R.A.(1913)"Studiesonthecultivationofthevirusofvaccinia"J.InfDis.13,
294300
3.TEDTalks.(2010,January21).AnthonyAtalaongrowingorgans[videofile].RetrievedMay15,2011from
(http://www.ted.com/talks/anthony_atala_growing_organs_engineering_tissue).
4.Bonner,J.(1936)."PlantTissueCulturesfromaHormonePointofView".Proc.Natl.Acad.Sci.22:426430.
doi:10.1073/pnas.22.6.426.JSTOR86579.PMC1076796 .PMID16588100.
5.Haberlandt,G.(1902)KulturversuchemitisoliertenPflanzenzellen.Sitzungsber.Akad.Wiss.Wien.Math.Naturwiss.
Kl.,Abt.J.111,6992.
6.No,A.C.(1934)."GottliebHaberlandt".PlantPhysiol.9(4):850855.doi:10.1104/pp.9.4.850.PMC439112 .
PMID16652925.
7.PlantTissueCulture(http://www.springer.com/life+sciences/plant+sciences/book/9783211838396).100yearssince
GottliebHaberlandt.Laimer,MargitRcker,Waltraud(Eds.)2003.SpringerISBN9783211838396
8.Martin,BerniceM."RoutineCellCulture."TissueCultureTechniques:AnIntroduction.Boston:Birkhuser,1994.29
30.Print.
9.Reece,JaneB.,LisaA.Urry,MichaelL.Cain,StevenA.Wasserman,PeterV.Minorsky,andRobertB.Jackson.
"AngiospermReproductionandBiotechnology."CampbellBiology.9thed.SanFrancisco,CA:BenjaminCummings,
2011.860.Print.

Externallinks
www.research.umbc.edu/~jwolf/method5.htm(http://www.research.umbc.edu/~jwolf/method5.htm)how
toguideforbiologystudents
PlantTissueCulture(http://www.accessexcellence.org/LC/ST/st2bgplant.html)wayto"xerox"aplant
CELOS(http://www.celos.sr.org/dept/Tissue%20Culture/index.asp)adivisionofCELOS(Centerfor
AgriculturalResearchinSuriname)
CellCultureBasics(http://www.invitrogen.com/site/us/en/home/References/gibcocellculturebasics.html)
Introductiontocellculture,coveringtopicssuchaslaboratorysetup,safetyandaseptictechniqueincluding
basiccellcultureprotocolsandvideotraining
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Categories: Histology Laboratorytechniques Cellculture
Thispagewaslastmodifiedon26August2016,at22:58.
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