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n
tp o
=
no to p
Where t = flow time
n/no= relative viscosity
In dilute solutions where pp0, the relative viscosity, nred, becomes t/to. The specific viscosity, nsp, is (t/to)
1. Therefore,
n =
n sp
c
Denaturant
HCl
NaOH
Urea
BME
SDS
NaOH
Blank
(Nativ
e)
1.12
1.15
1.09
1.11
1.17
1.14
time (min.)
Native
Blank
albumi
(Denatur
n
e)
1.73
1.2
2.02
1.22
2.12
1.42
1.86
1.6
1.91
1.78
2.08
1.26
Denature
d albumin
2.12
2.5
3.12
4.16
5.12
2.65
Nred Native
HCl
NaOH
Urea
Beta Mercaptoethanol
SDS
NaCl
54.46428571
75.65217391
94.49541284
67.56756757
63.24786325
82.45614035
0.5446428571
0.7565217391
0.9449541284
0.6756756757
0.6324786325
0.8245614035
Nsp
Denatured
0.7666666667
1.049180328
1.197183099
1.600000000
1.876404494
1.103174603
Nred denatured
76.66666667
104.9180328
119.7183099
160.0000000
187.6404494
110.3174603
For the denaturation of 1% albumin, Sodium Dodecyl Sulfate (SDS) is the denaturant with the highest
reduced viscosity with red=187.6404494. Based from the data, it can be concluded that the most effective
denaturant for 1% albumin is SDS followed by Beta Mercaptoethanol, Urea, NaCl, NaOH, and the least effective,
HCl.
References
Horton, H. Robert. Principles of Biochemistry. Upper Saddle River, NJ: Prentice Hall, 1996.pp.104-106 Print.
2013. Biochemistry Laboratory Manual. Biochemistry Academic Group, Institute of Chemistry, UP Diliman.
Philippines pp. 23-24
Appendix
Native
HCl; native sample
sp = (t/t0) -1= (Native albumin/ Blank (Native))-1
= (1.73/1.12) -1
sp = 0.5446428571
red = sp/ c
= 0.5446428571/0.01
red = 54.46428571
NaOH; native sample
= (2.02/1.15) -1
sp = 0.7565217391
red = sp/ c
= 0.7565217391/0.01
red = 75.65217391
Urea; native sample
= (2.12/1.09) -1
sp = 0.9449541284
red = sp/ c
= 0.9449541284/0.01
red = 94.49541284
Beta Mercaptoethanol; native sample
= (1.86/1.11) -1
sp = 0.6756756757
red = sp/ c
= 0.6756756757/0.01
red = 67.56756757
SDS; native sample
= (1.91/1.17) -1
sp = 0.6324786325
red = sp/ c
= 0.6324786325/0.01
red = 63.24786325
NaCl; native sample
= (2.08/1.14) -1
sp = 0.8245614035
red = sp/ c
= 0.8245614035 /0.01
red = 82.45614035
Denatured
HCl; denatured sample
sp = (t/t0) -1= (denatured albumin/ Blank (Native))-1
= (2.12/1.2) -1
sp = 0.7666666667
red = sp/ c
= 0.7666666667 /0.01
red = 76.66666667
NaOH; denatured sample
= (2.5/1.22) -1
sp = 1.049180328
red = sp/ c
= 1.049180328 /0.01
red = 104.9180328
Urea; denatured sample
= (3.12/1.42) -1
sp = 1.197183099
red = sp/ c
= 1.197183099 /0.01
red = 119.7183099
Beta Mercaptoethanol; denatured sample
= (4.16/1.6) -1
sp = 1.600000000
red = sp/ c
= 1.600000000
red = 160.0000000
SDS; denatured sample
= (5.12/1.78) -1
sp = 1.876404494
red = sp/ c
= 1.876404494/0.01
red = 187.6404494
NaCl; denatured sample
= (2.65/1.26) -1
sp = 1.103174603
red = sp/ c
= 1.103174603/0.01
red = 110.3174603