UNIT

VI
VI
VI
VI
VI
VI
VI
VI
VI
VI

PORTION
Mid Sem
Mid Sem
Mid Sem
Mid Sem
Mid Sem

QUESTIONNO
1
2
3
4
5
6
7
8
9
10

QUESTION
The efficiency of cell disruption in a bead mi
A common filter medium is the cloth filter ge
An ion exchange resin is composed of
The optimum wet solid content for the cell s

CHOICE A
concentration of the cells
canvas
polymeric network
5-15%

CHIOICE B
amount and size of beads
synthetic fabrics
ionic functional groups
15-30%

CHOICE C
type and rotational spee
metal or glass fiber
counter ions
30-60%

CHOICE D
all of these
all of these
all of these
60-90%

COR.ANS
D
D
D
C

EXP
-

SUBTOPIC
-

QUESTIONTYPE
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A
B
C
D
E

UNIT
I
I
I
I

PORT QUESQUESTION
CHOICE A CHIOICE B CHOICE C
Mid
1 Maintenance cost varies from____
12-16%
4-8%
0-4%
the filter,
dVf/dtthe
=dV
p/(
rate
/dt of
= (1/A)p/(
filtration
(1/A)
( dVf/dt = p/
Mid
2 Assuming the laminar flow across(1/A)
f
2
2
Mid
3 Filtration leaf test results indicate1.54
that ft
the filtration
3.08 rate
ft of a protein
65 ft2 product is 100 dry lbs/(ft
Mid
4 Selectivity can be expressed as ratio of the d mass fraction omass fraction

I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I

Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid

I
I
I
I
I
I
I
II
II
II
II
II
II

Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid

5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22

23
24
25
26
27
28
29
30
31
32
33
34
35

The disk centrifuge is the type of continuous olesser cost

higher speed
The effective of a solven can be Distribution Selectivity
Both A and B
The stage wise operation of adsor
Contact filtraConventional Affinity adso
Which of the following is not the Milling
Homogenizati Ultrasonicati
Conventional Adsorption is a
Reversible p Irreversible
Either reversi
1
0 >1
If the solute passes through memb
1
0 >1
If the membrane rejects all solute,
In antibiotic manufacturing proc 2to3
1 to2
4 to5
5
10
15
The conventional filtration invol
Which of the operation does no Media preparInoculum deveEffluent trea
6 to 7
For cost analysis in a fermentor 5 to 6
6 to10
Micro filtration refers to the se 0.02 to10m1 to 10Amstro 20 to 30 mic
10 to15
start up expenses in a fermentati0 to5
5to 10
The solvent flux across the membrane
J = Lp(p
can-be
)
J/A
expressed
= Lp(p as
- )
J=
(where
Lp/

Which of the following is conside Heating to d Addition of fil Addition of e

A typical tubular centrifuge has a 15
bowl
toof50
2-5 inch
50 to
diameter
100 and
30 inch height with a m
10090
toto
150
Concentration polarization c Pre filterin Reducing theBack washin
Assuming the laminar flow acro
(1/A) dVf/d dVf/dt = (1/A(1/A) dVf/d

Filtration leaf test results

indicate that the filtration
rate of a proteinproduct is
100 drylbs/(ft2hr). What
size filter would be required
toobtain 100 dry lbs of filter
cake/hr?
1.54 ft2
3.08 ft2
65 ft2
The filtration involves the s
5
10
15
Micro filtration is the separ 0.02 to 10 1-10A
20-30m
Primary steps in protein purifHomogeniza
Differential Solubilisati
Example of filter for continuoPlate and f Spiral woundRotary vac
During successful purificati specific actspecific acti number of p
Which of the following may be
NaCl
Protease inhiAMP
Disadvantage of enzymatic ce
Not always Not usually athe enzyme
In sonication method the rang
20-50KHz 60-80KHz
10-20KHz
Disadvantages of sonication heat generayield variabilfree radica
which is the physical methodsonication french press Homogeniz
which organic solvent is usedAcetonitrilecarbinol
Dichlorome
which enzymes are used in en
proteases lysozymes a and b

II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
II
III
III

Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid
Mid

36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61

In beadmill correct bead siz 0.1 to 6

2 to 7
0.5 to 9
Important consideration for opH
buffer
a and b
Methods for pre-treatment inHeating
Use of filter Flocculatio
Flocculation method will imp CentrifugatFiltration
Lyophilizat
Example of flocculating agenMnSo4
MgSo4
CaCl2
Polyelectrolytes
decreases tInert
Reduces the
. Ineffective flocculant for gl Bentonite Polystyrene Polyacryla
Particle size of filter aid is
2 to 20
10 to30
15 to 45
Which material has lower filt Cellulose Diatomaceousilica
Which type of radiation is us Gamma
Electron
Both a and
Which of the following is a pressur
Leaf filter (M Plate and frame
Rotary drum f
tubular bowl centrifuges as operate at employ bowl can not be
Two particles are called to be equsize.
specific gravit terminal velo
__________ centrifuge is normallTubular bowlDisc-bowl
Suspended b
Solid particles separation based oclarification classification elutriation
Diatomaceous earth is a/an
explosive
filter aid
filter medium
4
1
In washing type plate and frame fil
1/4
Ball mills and tube mills with flin asbestos
rubber
non-metallic
If a force greater than that of gra sedimentatioflocculation dispersion
increase the d
r ecrease the increase the
Filter aid is used to
Gravity settling process is not in hydrocyclon classifier
dorr-thickene
Range of compressibility co-effic 0.01 to0.1 0.1to 0.3
0.2 to 0.8
The efficiency of cell disruption concentrationamount and si type and rota
synthetic fabmetal or glas
A common filter medium is the clocanvas
An ion exchange resin is compospolymeric neionic functionacounter ions
15-30%
The optimum wet solid content fo5-15%
30-60%

CHOICE D COR.ANS
8-12%
C
dVf/dt = (1/A)p/
A
2
65 lbs/ ft .hr A
ratio of the A
ease in
operation

EXP

SUBTOPIC
0
0
0
0
0

QUESTIONTYPE
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)

B
Diffusivity C
Ion exchang A
Enzymatic diD
None of thesA
<1
B
<1
A
2 to4
C
20 B
Storage of r C
8 to 10
C
10 to 200 AmA
15 to 20
C
J/A = Lp/p A
All of the ab D
150 to 200 A
all of the D
dVf/dt = (1 A

E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)

65 lbs/ ft2 A
20 B
10-200A A
All of theseD
Tubular
C
both (a) an D
All of theseD
all the abo D
None of th A
all the abo D
decompressD
Toluene
D
none of th C

C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)

1A
buffer, tem D
All of the D
Drying
A
Caco3
C
certain antiD
All of the C
0.1 to 1
A
perlite
A
beta
C
Sand filter B
can't be us A
none of thes C
Perforated h C
sedimentatioB
catalyst
B
1/2 B
limestone C
centrifugatio D
act as a sup C
sedimentatioA
0.2 to 0.4
C
all of these D
all of these D
all of these D
60-90%
C

A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)
C (MODERATE)
C (MODERATE)
A (EASY)
B (MODERATELY EASY)
C (MODERATE)
D (MODERATELY DIFFICULT)
E (DIFFICULT)