DEPT.

OF BIOTECHNOLOGY ENGINEERING
KULLIYYAH OF ENGINEERING
INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA

Degree of BSc. In Biochemical-Biotechnology Engineering

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Mid semester Exam
Semester 2, 2014/2015
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MOLECULAR BIOLOGY AND GENETIC ENGINEERING
(BTE 3433)
Time allowed 1 hour

Answer ALL questions.

Answer ALL questions.

Q1. [14 marks]
a.

Name organelle that contain the following structures:

(3 marks)

i. Cristae
ii. cisternae
iii. thylakoid
b. Which organelle has the following functions

(3 marks)

i. sorting and shipping products of the endoplasmic reticulum

ii. digest macromolecules
iii. store organic & inorganic compounds
c. Describe about:

(4 marks)

i. Collagen

ii. Proteoglycan

d. State one difference between:

i.

Hemidesmosome and desmosome

ii.

Adherence junction and synaptic junction

(4 marks)

Q2. [11 marks]

a. The following are short double stranded DNA fragment of liver cells (the bold sequence is exon and
the non-bold sequence is intron):
5 atg att atc gac gtg cgc tat att ggc cct ggt aaa ggg act 3
3 tac taa tag ctg cac gcg ata taa ccg gga cca ttt ccc tga 5

i.

What are the resulting strands when the above strand is duplicated?

(2 marks)

ii.

With the aid of diagram, discuss the replication of the discontinuous DNA strand.
(6 marks)

iii.

Briefly explain the posttranscriptional modifications occur to mRNA transcript of

eukaryotic cells?
(3 marks)

Q3. [10 marks]

a. What is the unique biological process occur in:
(2 marks)
i. metaphase I of meiosis that do not happen in metaphase of mitosis?

ii. anaphase I of meiosis that do not happen in anaphase of mitosis?

b. A group of scientist has discovered that animal X with 2n=14 chromosome. What is
the total no of chromosomes when the cells of that animal in the following stage:
(4 marks)
i) G2 phase
ii) After mitosis
iii) After meiosis I
iv) After meiosis II
c. The scientist discovered a species with 2n = 4. Draw a figure showing the stages of
mitosis of that species.
(4 marks)