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Introduction to Metabolism
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OUTLINE
I.
II.
III.
IV.
V.
VI.
VII.
Learning Objectives
Metabolism
Metabolic pathways
Glucose metabolism
Protein metabolism
Lipid metabolism
Metabolic pathways may be studied at different levels of organization
A.
Integration of metabolism at the tissue level & organ level
B.
Integration of metabolism at the cellular level
VIII. Metabolites must be regulated in a concerted manner
IX.
Metabolic fuel reserves are mobilized in the fasting state
X.
In the fed state
XI.
Patterns of metabolic regulation
I.
A.
B.
LEARNING OBJECTIVES
General Objectives
To explain thoroughly how cells carry out and regulate complex
reaction sequences.
Specific Objectives
1. To be able to differentiate between anabolic and catabolic
pathways
2. To be able to explain briefly how carbohydrates, lipids and
proteins are metabolized
3. To be able to correlate relationships between each
pathways
Intermediary Metabolism
o Reactions involving the low molecular weight molecules
that are metabolites in the degradation and/or
biosynthesis of biopolymers.
Energy metabolism
o Part of intermediary metabolism
o pathways that store or generate metabolic energy
o Most organisms derive both the raw materials and the
energyfrom organic fuel molecules such as glucose.
o reducing equivalents released by CAC stored in NAD and
FADH2
II. METABOLISM
Categories:
Catabolism
o From complex to simpler organic molecules
o Often exergonic (produces energy)
o Eg: Degradation of CHON to AA
Triglycerides to fatty acids
Glucose to pyruvate
Anabolism
o Synthesis of simple to complex organic molecules for the
maintenance of growth and reproduction
o Endergonic in nature
o Eg: Glycogenlolysis
Amphibolic
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Introduction to Metabolism
July 28,2011
C.
D.
E.
F.
V. PROTEIN METABOLISM
Pathways present:
A. Glycolysis
o Can occur anaerobically (oxygen absent) but instead of
glucose, the product will be lactate
o Linked to oxidative phosphorylation and thus, also
producing ATP
B. Pentose phosphate pathway
Group 17|Hidalgo, Homol, Inandan, Inciong, Jeanjaquet, Juen, Quanico
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Introduction to Metabolism
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Fatty Acids
o Exist in diet as triglycerides
o Stored in Adipose Tissue
o Sources
Dietary lipid
Amino acids and glucose- absorbed via the hepatic portal vein
Liver
o Regulates blood concentration of water-soluble
metabolites via:
Glycogenesis
Lipogenesis
Glycogenolysis
Gluconeogenesis
o Synthesizes major plasma proteins (albumin)
o Deaminates amino acids to urea
Kidney
o Where urea from liver is transported and then excreted
Skeletal muscle (for muscle contraction)
o Fuel: glucose (storage form: glycogen)
o Product: lactate and CO2 (anaerobically)
o Muscle protein from plasma amino acids: 50% body mass,
a possible source of energy during starvation
Lipids
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Introduction to Metabolism
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B.
Subcellular Level
Cytosol
Glycolysis
Gluconeogenesis
Pentose phosphate pathway
Fatty acid synthesis
Protein synthesis in ribosome
catabolism
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In fasting state
o concentration of glucose in the portal blood falls
o insulin decreases
o skeletal muscle and adipose tissue take up less glucose
o increase in secretion of glucagon inhibits glycogen
synthetase, and activates glycogen phosphorylase in the
liver
Fasting state in adipose tissue
o decrease in insulin and increase in glucagon results in
inhibition of lipogenesis, inactivation of lipoprotein lipase,
and activation of intracellular hormone-sensitive lipase
o leads to release from adipose tissue of increased amounts
of glycerol and free fatty acids
Fasting state in Muscle
o preferentially takes up and metabolizes free fatty acids
Fasting state in Liver
o greater capacity for B oxidation
o fasting becomes more prolonged, it forms more acetylCoA
o acetyl-CoA is used to synthesize the KETONE BODIES
Prolonged Starvation
o glucose may represent less than 10% of whole body
energy-yielding metabolism
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Introduction to Metabolism
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Erythrocytes
o lack mitochondria rely on (anaerobic) glycolysis and the
pentose phosphate pathway
Brain
o metabolize ketone bodies to meet about 20% of energy
requirements
o remainder must be supplied by glucose
In pregnancy
o fetus requires a significant amount of glucose and
synthesis of lactose in lactation
Allosteric modification
o Feedback inhibition when the product controls the rate
of its own synthesis
o Feedforward activation when a metabolite produced
early in the pathway activates an enzyme that catalyzes a
reaction further down the pathway.
Covalent modification alters catalytic rate by attachment to
some group by a covalent bond (usually a phosphate group
o Phosphorylation:
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