Materials & Streams Report

October 27, 2016

for EJEMPLO FERMENTADOR

1. OVERALL PROCESS DATA
Annual Operating Time
Recipe Batch Time
Recipe Cycle Time
Number of Batches per Year
MP = Undefined

7,903.23h
32.52h
32.52h
243.00

- Page 1 -

2.1 STARTING MATERIAL REQUIREMENTS (per Section)

Section

Starting Material Active Product

Amount
Needed
(kg
Sin/kg
MP)

Fermentacin

(none)

Unknown Unknown Unknown Unknown

(none)

Molar
Yield
(%)

Mass
Yield
(%)

Gross
Mass
Yield
(%)

Sin = Section Starting Material, Aout = Section Active Product

2.2 BULK MATERIALS (Entire Process)

Material
Water
Biomass
Medio
Air
TOTAL

kg/yr

kg/batch

1,895,400
1,215
91,125
1,835,732
3,823,472

7,800.000
5.000
375.000
7,554.454
15,734.454

kg/yr

kg/batch

1,895,400
1,215
91,125
1,835,732
3,823,472

7,800.000
5.000
375.000
7,554.454
15,734.454

kg/kg MP

2.3 BULK MATERIALS (per Section)

SECTIONS IN: Reaccin de fermentacin
Fermentacin
Material
Water
Biomass
Medio
Air
TOTAL

kg/kg MP

- Page 2 -

2.4 BULK MATERIALS (per Material)

Water
Procedure
% Total
Fermentacin (Reaccin de fermentacin)

kg/yr

kg/batch

100.00
100.00

1,895,400
1,895,400

7,800.000
7,800.000

Biomass
Procedure
% Total
Fermentacin (Reaccin de fermentacin)

kg/yr

kg/batch

P-1
TOTAL

100.00
100.00

1,215
1,215

5.000
5.000

Medio
Procedure
% Total
Fermentacin (Reaccin de fermentacin)

kg/yr

kg/batch

100.00
100.00

91,125
91,125

375.000
375.000

Air
Procedure
% Total
Fermentacin (Reaccin de fermentacin)

kg/yr

kg/batch

1,835,732
1,835,732

7,554.454
7,554.454

P-1
TOTAL

P-1
TOTAL

P-1
TOTAL

100.00
100.00

kg/kg MP

kg/kg MP

kg/kg MP

kg/kg MP

2.5 BULK MATERIALS: SECTION TOTALS (kg/batch)

Raw Material
Water
Biomass
Medio
Air
TOTAL

Fermentacin
7,800.000
5.000
375.000
7,554.454
15,734.454

- Page 3 -

2.6 BULK MATERIALS: SECTION TOTALS (kg/yr)

Raw Material
Water
Biomass
Medio
Air
TOTAL

Fermentacin
1,895,400
1,215
91,125
1,835,732
3,823,472

- Page 4 -

3. STREAM DETAILS
Stream Name
Source
Destination
Stream Properties

Agua
INPUT
P-1

Medio
INPUT
P-1

Aire
INPUT
P-1

Venteo
P-1
OUTPUT

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

0.00
25.00
1.01
994.70
0.00
0.00
1.00

0.00
25.00
1.01
995.39
0.00
0.00
1.00

0.00
25.00
1.01
1.18
0.00
0.00
0.24

0.00
37.00
1.01
1.14
34.18
3.89
0.24

0.000
0.000
0.000
0.000
0.000
7,800.000
7,800.000
7,841.526

5.000
0.000
375.000
0.000
0.000
0.000
380.000
381.758

0.000
0.000
0.000
5,795.156
1,759.297
0.000
7,554.454
6,406,305.316

0.000
262.463
0.000
5,802.648
1,499.108
0.000
7,564.220
6,615,792.539

Component Flowrates (kg/batch)

Biomass
Carb. Dioxide
Medio
Nitrogen
Oxygen
Water
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 5 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Caldo
P-1
OUTPUT
0.00
5.00
0.82
1,002.61
- 189.80
- 19.96
1.00

Component Flowrates (kg/batch)

Biomass
Impureza
MAb
Water
TOTAL (kg/batch)
TOTAL (L/batch)

110.000
37.500
7.500
8,025.000
8,180.000
8,158.700

- Page 6 -

4. OVERALL COMPONENT BALANCE (kg/batch)

COMPONENT
Biomass
Carb. Dioxide
Impureza
MAb
Medio
Nitrogen
Oxygen
Water
TOTAL

INITIAL

INPUT

OUTPUT

FINAL

IN-OUT

0.000
0.000
0.000
0.000
0.000
8.302
2.520
0.000
10.822

5.000
0.000
0.000
0.000
375.000
5,795.156
1,759.297
7,800.000
15,734.454

110.000
262.463
37.500
7.500
0.000
5,802.648
1,499.108
8,025.000
15,744.220

0.000
0.037
0.000
0.000
0.000
0.810
0.209
0.000
1.056

- 105.000
- 262.500
- 37.500
- 7.500
375.000
0.000
262.500
- 225.000
0.000

- Page 7 -

5. EQUIPMENT CONTENTS
FR-101
Procedure

Operation

P-1
P-1
P-1
P-1
P-1
P-1
P-1

START
CHARGE-1 (Charge)
CHARGE-2 (Charge)
HEAT-1 (Batch Heating)
FERMENT-1 (Batch Kinetic Fermentation)
COOL-1 (Batch Cooling)
TRANSFER-OUT-1 (Transfer Out)

Time (in h)

Volume (in L)

0.000
1.390
2.357
2.840
26.840
27.990
32.524

0.000
7,841.526
8,223.285
8,259.583
8,253.558
8,158.700
0.000

- Page 8 -