Name: Titan

Lab Partners:
Name: Harry
Name: Kha
Name: Noah
Biology Fall 2016-Period 3
Mr. Bill Pham
Date:28/10/2016

Effect of
Temperature on
Enzyme
Activity
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Introduction:
Enzyme is a reaction that speeds up the rate of a chemical reaction that occurs in a living
organism. Enzyme has many important roles in biological processes. In fact human and many
other living organisms cannot exist if they are lack of enzyme because the chemical that required
to maintaining the function of human body and other living things are not occur fast enough.
This is also the reason that make enzyme so important. An example of enzyme important role in
a biological process is that when you have a fever your body starts to feel hot, the reason is that
enzyme speed up the temperature in your body to kill the virus. Another example is that enzyme
keeps the blood flow steady to many important organisms in your body and keeps it function the
right way. If there is no enzyme activity occurs in your body the blood will not flow fact enough
to keep your body function the right way. There are many factors that can affect the enzyme
activities, two of them are pH and temperature. In this lab experiment, the purpose is to find out
how temperature affects the enzyme activities. When increasing the temperature, it also increases
the speed of enzyme activities.
According to Scientificamerican , In our liver, as within other tiny tissues in the body, many
chemical reaction happen. These reaction usually need support to speed up there process. That
when enzyme join the work to help our liver and other tiny tissues.
Quoting from Pubchem, Hydrogen peroxide is a strong oxidizing agent used in aqueous
solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and
solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic
materials. (National Center for Biotechnology Information)
Hydrogen peroxide is toxic to you because it unstable and solutions, it is highly dangerous for
organic material. If hydrogen peroxide does not break down and stay you touch it. Later it will
interact with the cells and molecules and damage them. Liver is work as a cleaning system to our
body because of this the enzyme in liver can break down hydrogen peroxide and make this
become less harmful.
Our group has a hypothesis that because when temperature hotter the enzyme activities also get
speed up so the cooler the temperature the slower the enzyme activities occur.

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Material:
-Stop watch

-Gloves

-3 Thermometers

-Lab Coat

-Warm Water Bath

-25 ml-graduate cylinder

-Cold Water Bath

- Petri Dishes

-Room Temperature Water Bath

-Liver Puree

-Filter Paper

-Paper Towel

-Hydrogen Peroxide
-Forceps

Procedure:
Cold Water Bath:
Step 1: Prepare lab gloves, lab coat and hydrogen peroxide, liver puree, filter paper, paper towel,
stop watch, thermometer, and forceps.
Step 2: Wear your lab gloves and lab coat on before you start the experiment.
Step 3: Prepare liver puree and put the liver puree into the cold water bath.
Step 4: Use the thermometer to measure the temperature then write down the data onto your lab
note book.
Step 5: Prepare a small amount of hydrogen peroxide.
Step 6: Use the filter paper a dip it into the liver puree then dry it with paper towel.
Step 7: Put the filter paper with puree on it and dip it into the hydrogen peroxide.
Step 8: Start the stop watch, and wait until the filter paper flow up to the surface and stop the
stop watch.
Step 9: Write down the data into your lab notebook.
Step 10: Repeat the experiment two more times and compare the data.
Room Temperature Bath:
Step 1: Prepare lab gloves, lab coat and hydrogen peroxide, liver puree, filter paper, paper towel,
stop watch, thermometer, and forceps.
Step 2: Wear your lab gloves and lab coat on before you start the experiment.
Step 3: Prepare liver puree and put the liver puree into the room temperature water bath.
Step 4: Use the thermometer to measure the temperature then write down the data onto your lab
note book.
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Step 5: Prepare a small amount of hydrogen peroxide.

Step 6: Use the filter paper a dip it into the liver puree then dry it with paper towel.
Step 7: Put the filter paper with puree on it and dip it into the hydrogen peroxide.
Step 8: Start the stop watch, and wait until the filter paper flow up to the surface and stop the
stop watch.
Step 9: Write down the data into your lab notebook.
Step 10: Repeat the experiment two more times and compare the data.

Warm Water Bath:

Step 1: Prepare lab gloves, lab coat and hydrogen peroxide, liver puree, filter paper, paper towel,
stop watch, thermometer, and forceps.
Step 2: Wear your lab gloves and lab coat on before you start the experiment.
Step 3: Prepare liver puree and put the liver puree into the warm water bath.(Notice: The water
cannot be hotter than 40 degrees Celsius)
Step 4: Use the thermometer to measure the temperature then write down the data onto your lab
note book.
Step 5: Prepare a small amount of hydrogen peroxide.
Step 6: Use the filter paper a dip it into the liver puree then dry it with paper towel.
Step 7: Put the filter paper with puree on it and dip it into the hydrogen peroxide.
Step 8: Start the stop watch, and wait until the filter paper flow up to the surface and stop the
stop watch.
Step 9: Write down the data into your lab notebook.
Step 10: Repeat the experiment two more times and compare the data.

Data & Result:

This is our data that we record from the lab experiment
Trial
Temp (oC) Time (sec)
RT 1
26
9
RT 2
26
8
RT 3
26
5
Average 26
7.33
Cold 1

5
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Cold 2 10
Cold 3 10
Average 9.67

12
8
8.33

Hot 1
Hot 2
Hot 3
Average

5
7
6
6.00

30
30
30
30

Cold (Ave)
RT (Ave)
Hot (Ave)

9.67
26
30

8.33
7.33
6.00

When we put the livers enzyme at room temperature into the hydrogen peroxide it has a normal
amount of bubbles. When we put the cold livers enzyme into hydrogen peroxide, it reacts
slower and has fewer bubbles than the room temperature. Finally we put the hot livers enzyme
into hydrogen peroxide; the enzyme activities occur much faster than the room temperature and
also produce more bubbles than the cold and room temperature enzyme.

Graph:

Effect of Temperature On Enzyme Activity

9.00
8.00
7.00

Time (sec)

6.00
5.00
4.00

Series1

3.00
2.00
1.00
0.00
0.00

5.00

10.00

15.00

20.00

25.00

30.00

35.00

Temperature (oC)

Discussion:
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The line graph presents the data on temperature effect on enzyme activities. It compares the
speed of the livers enzyme between three different groups of temperature. The groups are cold
temperature, room temperature and hot temperature. As you can see the enzyme activities are
slow in cold temperature and become faster as the temperature become warmer and warmer. The
purpose of graphing this data is to show the trend of the data that we record in lab this also
present the information more clearly and help us to easy to understand. This graph also supports
our group hypothesis because as the temperature increases the speed of the enzyme activities also
increase. The factor that could affect our result is the temperature of the livers enzyme. If it too
hot the enzyme in liver will stop working. So we need to keep the temperature steady. We need
to repeat each experiment 3 times, for each temperature. The reason is that the first and second
time are variable, so we need the third time to make sure, this also help us to calculate the
average more easy. We need to the average of each reaction because we want to know the
average speed of each reaction and how fast they usually occur.

Research Question:
According to Livescience, Enzyme works as a machine to break down large molecules so that it
is easier for the body to absorb. Some other enzyme combines two molecules together to form
new molecules. Each enzyme only speeds up a specific reaction. Substrate are the molecules that
enzyme works with. Substrate combines with enzyme by the enzyme active site. There are two
theories to explain the interaction of the enzyme substrate. The first one are lock and key and the
second one is the induced fit-model.
Quoting from Quizlet, A change in pH could change the shape of hexokinase. This change
would diminish or possibly eliminate the ability of glucose and ATP to bind to the active site on
the enzyme.(Emma_Barnes6)

Reflection:
The new techniques that I learn from this second lab experience is that how to test livers
enzyme with hydrogen peroxide. I also learn how to use the filter paper. I also learn from this lab
the harmful effect of the hydrogen peroxide. From this lab, I have understood more about
enzyme activities. The aspect from the experiment that I dislike is that the smell of the liver. The

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liver smells very stinky because it not cooks yet. The aspect that I like from this lab is that I can
observe the reaction of the livers enzyme when it meets the hydrogen peroxide. The experiment
is not difficult but we have to be careful with the hydrogen peroxide. It was easy because the
procedures are doable and the reaction also easy to understand. In my opinion, my team is well
collaborate. Example of it is that we divided the job fairly. One of use will prepare the liver
puree, one will prepare the hydrogen peroxide, one will prepare for the temperature of the liver
puree and the last one will have responsible for the reaction time and note the data. We can
improve this lab experiment by study more and to be more careful on handle the lab equipment
and chemical.

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Works Cited
"Enzymes." - Biology Encyclopedia. N.p., n.d. Web. 30 Oct. 2016.
< http://www.biologyreference.com/Dn-Ep/Enzymes.html>

Buddies, Science. "The Liver: Helping Enzymes Help You!" Scientific American. N.p., n.d.
Web. 30 Oct. 2016.
<https://www.scientificamerican.com/article/bring-science-home-liver-helping-enzymes/>.

"Hydrogen Peroxide | H2O2 - PubChem." National Center for Biotechnology Information. U.S.
National Library of Medicine, n.d. Web. 30 Oct. 2016.
<https://pubchem.ncbi.nlm.nih.gov/compound/hydrogen_peroxide#section=Top>.

"The Effect of Hydrogen Peroxide on Liver: Hypothesis, Apparatus, Method | Online Homework
Help | SchoolWorkHelper." Online Homework Help SchoolWorkHelper. N.p., n.d. Web. 30 Oct.
2016.
<http://schoolworkhelper.net/the-effect-of-hydrogen-peroxide-on-liver-hypothesis-apparatusmethod/>

Castro24, Joseph. "How Do Enzymes Work?" LiveScience. TechMedia Network, 24 Apr. 2014.
Web. 30 Oct. 2016.
<http://www.livescience.com/45145-how-do-enzymes-work.html>

"Biology Chapter 7.1 and Chapter 2." Flashcards. N.p., n.d. Web. 30 Oct. 2016.
<https://quizlet.com/100199555/biology-chapter-71-and-chapter-2-flash-cards/>.

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