Documente Academic
Documente Profesional
Documente Cultură
Immunohematology
Division of hematology concerned with immune, or antigen-antibody reactions, and
with related changes in the blood
Transfusion Medicine
Branch of medicine that is concerned with transfusion of blood and blood components
I.
1492
I.
- Blood group antigens and antibodies bind until dynamic equilibrium is reached.
- Represented by bell shaped curve based on concentration. (Zone of Equivalence)
Prozone: excess of unbound immunoglobulin; antibody excess
Postzone: surplus of antigen; excess antigen
Zone of equivalence: optimum reaction occurs
1. Agglutination / Hemagglutination
RBCs have antigens which combine with antibodies present in serum (either patient or reagent).
Antibody bridges form with antigens on adjacent cells resulting in agglutination.
2. Hemolysis caused by antibody binding, activation of complement which results in destruction of
RBC membrane.
TWO stages involved
1. First stage is sensitization, attachment of an antibody to corresponding antigen on RBC
membrane.
2. Second stage is lattice formation, bridges form between sensitized cells.
a. IgG have 2 antigen binding sites, one binds to antigen on one cell, the other binds to
antigen on adjacent cell.
b. IgM has 5 antigen binding sites and can bind to several antigen sites on different cells.
Lattice formation will result in hemagglutination.
Zeta Potential Theory: RBC surface has sialic acid molecules
In saline, cations are attracted to negative red cell surface forming a repelling cloud around the
cell; Electric repulsion
Potentiator: enhance antigen and antibody reactions (ex. LISS)
Lectins/Prolectins: Antibody-like substance derived from plant extract
Lectin specificity
Anti-A1
Anti-B
Anti-H
Anti-M
Anti-N
Prolectin specificity
Anti-A1
Anti-A2
Anti-B
Anti-H
Plant source
Dolichos biflorus
Banderaea simplicifolia
Ulex europaeus
Iberis amara; I. umbellata, I. semperivens
Madura aurantiaca
Vicia graminea; Bauhinia variegate; B. candicans
Snail source
Helix pomatia; H. aspersa, Cepaea nemoralis
Euphrada periomphala; Bradybaenafruticum spp.
Salmo irideus
Eel