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chemical scissors to
cut backbone of DNA
between deoxyribose
sugar and phosphate
groups at or near
specific recognition
sites.
Majority have been
isolated from bacteria
Importance:
A method used to
separate and measure
the molecular size of
DNA and RNA
molecules using
electric field.
The migration rates of
DNA molecules are
inversely proportional
to their molecular
weights.
A standard curve
can be obtained by
plotting the
molecular size of
the fragments of
the marker against
the their respective
mobility.
The molecular size
of the test sample
can be obtained
from the standard
curve.