Zaheraa Ramsahoye

Pd:2
12/16/16
Ms. Leno.

The result that occurs with enzyme catalase control and experimental trial.
During the lab process our team conduct an enzyme activity experiment. An enzyme
is known as protein that speeds up chemical breakdown of food. There are many
different types of enzymes that help catalyze chemical reaction. In other words, proteins
increase the rate of reaction. The experiment is based on the enzyme catalase to
investigate how pH will affect the reaction causing it to increase as it breaks down
hydrogen peroxide. Then, the H2O2 is decomposed to water and oxygen. In the lab
procedure our team came up with a hypothesis predicting that if the pH buffer of 7 is
added to the H2O2 of 1.5% then, it would increase the rate of reaction. Which typically
meant that our prediction could change as we increase the pH buffer to a larger amount
into the H2O2.
Catalase Lab Procedure:
Computer with Internet access and Vernier LoggerPro software
LabQuest Mini
Vernier Gas Pressure Sensor

Two-hole black rubber stopper (top)

Plastic tubing with Luer-lock connectors
20-200 L micropipette set to 100 L
micropipette tips
50mL graduated cylinder
250 mL Erlenmeyer flask
Magnetic stirrer
Stirring bar
Thermometer
Metal clamp stand
At the central table:
Hydrogen peroxide
Catalase enzyme suspension
Check that every person is wearing apron and goggles.

_Check that everyone who will touch the enzyme or liquids is

wearing thick latex gloves (blue and yellow gloves)
The Task:
Open your laptop and open Logger Pro .
Click File Open and open the folder in Biology by
Vernier. Then choose 06 Enzyme (Pressure).
Connect the LabQuest Mini to the computer using the USB
cable, and connect the Gas Pressure Sensor to CH 1 of the LabQuest Mini.
Set up the laboratory apparatus as seen in the picture:
Measure out 50mL of 1.5% H2O2
and pour into an Erlenmeyer flask.
Carefully place a magnetic stir bar in
the flask.

Place the flask on a magnetic stir

plate. Use a clamp to fasten the flask to the ring
stand as shown. Position the flask at the center
of the magnetic stirrer.
Test the stirrer at 100 rpm.
Stop the stirrer.
Use the plastic tubing with two Luerlock connectors to connect the two-hole rubber
stopper assembly to the Gas Pressure Sensor as shown in the image. The
valve connected to the stopper should stay closed during this investigation.
Its closed when its flat, parallel to the floor
Complete all the steps below quickly to complete your test reaction.
Start data collection: click green Collect button on Logger Pro.
Using a micropipette, add 100 L of enzyme suspension to the
contents of the flask.

IMMEDIATELY tightly seal the flask by placing the stopper in and

HOLDING it in carefully.
IMMEDIATELY Turn the stir plate on to 100.
NOTE: If the pressure exceeds 130 kPa, the pressure inside the flask will be
too great and the rubber stopper will likely pop off. HOLD DOWN the
stopper or be ready to have uncovered chemicals on your desk
WAIT 200 seconds (3.3 minutes) while data is
collecting- Do NOT click STOP. Data collection will automatically stop
after 200 seconds.
Turn off the stir plate.
Carefully remove the stopper from the flask to relieve the
pressure.
Use a thermometer to test and record the temperature of the
liquid in your lab packet

Pour all chemical waste into a RED bucket.

Remove the magnetic bar.
Dispose of your used micropipette tip.
23. ___all_______Take off your safety gear and place it neatly away.
24. Observe the graph generated using LoggerPro software (example shown
below).
25. Hold down Control on the keyboard and press j to zoom in the graph.
26. Highlight the section of the graph where the slope is increasing, by clicking
and dragging your mouse across it.
27. Click the Analyze tab at the top of the page, and choose Linear Fit. A
statistics box will appear for your highlighted section of the graph.
28. Record the slope of the line, m, as the rate of catalase activity in kPa/s in
your lab packet (page 13)

29. Click File, Save As, and save with the file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Logger Pro Data
Email the graph to Ms. Lenowitz by following these final steps!
30. Click File and choose Print Graph. In the drop down menu of printers, choose
32. Open your MESA email and Compose a new email to
glenowitz@mesacharter.org
33. Attach the PDF to the email.
34. Press send!

Data Analysis:

The result of the experimental trial supported the original hypothesis which is if the pH
buffer of 7 is added to the H2O2 1.5% then, it would increase the rate of reaction. In the
experimental trial there were a higher slope of ( m=0.01347 kPA/min) due to the amount
of pH that was added to the hydrogen peroxide to increase the reaction. While, the first
control trial wasnt accurate because of the slope of (m= -0.1249 kPa/min) maybe it is
due to the amount of pressure that cause the slope went negative reaction. The result
make sense and was accurate because the hydrogen peroxide and pH buffer with a higher

amount causes the enzyme and H2O2 increase in the gas pressure . In the end, the lab
procedure went smoothly until the group had decided not to support as a team to find the
accurate slope for the trial to see if the reaction would go higher or lower.