Basic Operating Instructions for

Autoclave
1. Place the items to be autoclaved in the
chamber.
2. Check and fill the reservoir with water to
the fill line.
3. Close and seal the autoclave door.
4. On the autoclave keypad, select the
sterilization time:
a. The sterilization time:
The sterilization time will vary
according to the contents and how the
load is packaged and should be
measured after the temperature of
the materials reaches 121C and 15
pounds per square inch (PSI).
It can be variable with a minimum of
12-15 minutes. Several trays with large
bags or containers loaded in the
autoclave will require a longer time to
reach 121C and should be set
accordingly.
Run the autoclave cycle. Fill out the
autoclave log if this is required.
5. At the completion, wear lab coat with long
sleeves, closed-toed shoes, and heatresistant gloves (if required).

6. Open the door slowly and only slightly and

allow steam to escape.
7. Allow items to cool in the autoclave for at
least 10 minutes before fully opening the
door.
8.
In case of any emergency/ help, contact
lab assistant and in-charge immediately.