Documente Academic
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Documente Cultură
BUCK S. SAMUEL
Assistant Professor
Alkek Center for Metagenomics and Microbiome Research
Baylor College of Medicine
bucks@bcm.edu | @microbeMinded
Diseased
Outline
C. elegans as a high-throughput gnotobiotic system
Outline
C. elegans as a high-throughput gnotobiotic system
PROLIFERATING
adult
L4
apple
L1
petasites
apple
orange
optunia
grapes
apple
bryony
slug
snail
NON-PROLIFERATING PROLIFERATING
L2d
apple
dauers
snail
L2
L3
Apple Orchard, Orsay, France
various invertebrates
[invertebrate dispersal]
embryos
dauer
hawthorn
Marie-Anne Felix
Highest colonization in
early adulthood
Meta analysis of Samuel et al., PNAS (2016); Dirksen et al., BMC Biol (2016); Berg et al., AEM (2016)
Meta analysis of Samuel et al., PNAS (2016); Dirksen et al., BMC Biol (2016); Berg et al., AEM (2016)
IMPACT ON
GROWTH/METABOLI
SM
Growth rates
Brood delivery
Fat storage
Lifespan
INDUCTION OF
PATHOGEN
REPORTERS
INDUCTION OF STRESS
REPORTERS
hsp-6p::GFP (mitochondria)
hsp-4p::GFP (ER)
dhs-9p::GFP (proteosome)
BACTERIAL
irg-1p::GFP (bZip)
F35E2.5p::GFP (p38 MAPK)
clec-60p::GFP (Wnt)
PATHOGEN or
COMMENSAL?
VIRAL
RNAi effectiveness
sur-5p::GFP (Tg silencing)
scm::GFP (hairpin silencing)
Healthy
Diseased
Many thanks!!
BCM/TMC, labs of:
Joseph Petrosino
Rob Britton
Gretchen Diehl
Joseph Hyser
Lynn Zechiedrich
Mary Estes
Gary Ruvkun
SAMUEL LAB:
Christopher Ayoub
Fan Zhang, Ph.D.
Ali Strtak
Jessica Weckhorst
Collaborators:
Marie-Anne Felix (INRS)
Hinrich Schulenburg (U Kiel)
Michael Shapira (UC Berkeley)
Katherine Gregory (Brigham and Womens Hospital)
Allan Walker (MGH)