Documente Academic
Documente Profesional
Documente Cultură
IgA+
Th17
CD103+ DC
Treg
commensalspecific Tregs
MLN
Treg
Lamina
Propria
CD103+ DC
mucosal firewall
commensalspecific plasma
B cell
Physical Barrier
- Epithelia/Tight Junctions
- Mucus Layer
- Anti-Microbial Peptides
Active Barrier
- Tolerogenic DCs
- Tregs
- IgA+ B cells
peripheral
lymphoid
organs
B
B
Teff
N
Teff
Teff
Teff
Stomach
Ulcers and stomach cancer
Lung
Cystic Fibrosis
Skin:
Psoriasis
Atopic dermatitis
Inflammation
1
TNF
Dendritic
cell
IFN
Inflammatory
Monocyte
Neutrophil
T cell
Lamina propria
TNF
Adipose tissue
Effector
T cell
2
Fonseca, Hand et al.
(2015) Cell
3
mesLN
Education: Administration
Need to get buy-in from the Senior Administration
- Will provide you with start-up for facility as well as subsidy to
keep facility running
Education: Community
What are gnotobiotic animals?
How are they made? (This one is critical)
What are the advantages and limitations of these
animals?
How do we physically carry out the experiments and
how does that affect experiment design? (need to
explain the isolators etc.)
This is also an ad hoc recruitment effort because we
need people to use the facility
Gnotobiotic Isolators
Sequencing
MiSeq x 2
NextSeq x 2
Acknowledgements
Pitt
Mark Shlomchik
Mark Gladwin
Terry Dermody
Art Levine
Alison Morris
Joe Newsome
Jay Kolls
NIH
Yasmine Belkaid
Funding:
UAB
Trent Schoeb
Chicago
Alexander Chervonsky