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RAW

FERMENTATION OF WINE
STOCK SHAKE SEED RAW MATERIALS STEARILIZATION
MATERIAL
CULTURE FLASKS FERMENTER GRAPES 1. Boiling
1. GRAPES YEAST 2. Chlorination
2. YEAST Some microbes are SULFUR 3. Iodine
3. SULFUR seeded into fed-batch DIOXIDE
DIOXIDE fermenter CANE OR BEET
4. CANE OR SUGAR
BEET
MEDIUM FORMULATION
SUGAR
1 GALLON OF GRAPES
FERMENTATION
1.5 GALLON OF WARM WATER
1 CUP SUGAR
10 g OF DRY YEAST
Fed-Batch fermentation

alternative mode of operation for


fermentations leading with toxic
PROCESS OF WINEMAKING substrates or low solubility
compounds

FERMENTER
1. Mix the content with the
rising bubbles which
provide oxygen for the cell
2. Do not require agitation
3. Energy efficient

3 STEPS OF PURIFICATION RECOVERY SECTION


1. FINING 1. FILTRATION
- fining agent is added to the wine to create - Membrane filter press. The cake
an adsorbent to produce larger molecules that chambers are covered with a rubber
will precipitate out of the wine more rapidly membrane that is inflated using air
2. FILTRATION or water, allowing in situ compacting
- series of filtering. the wine through a filter medium of the cake. These allow higher yield
that captures particles larger than the medium's holes and drier cake
3. FLOTATION 2. CENTRIFUGATION
- small bubbles are injected at the bottom tank
- As the bubbles rise through the must, grape Solids
solids, including phenolic compounds prone to discharge
oxidation and browning. This must be done centrifuge
prior to fermentation, since yeast will inhibit the
flocculation involved. Solids ejecting centrifuges have continuous
or intermittent mechanisms to discharge
solids and may be used for the recovery of
mycelia or bacterial biomass

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