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Study Questions
1. Define or explain the following terms:
Semiconservative DNA replication conservative DNA replication
Protein enzyme
Transcription translation
1
rho-independent termination transcription factors
Exon 5 cap
2. Describe the steps involved in DNA replication, include the enzymes and/or
proteins involved.
3. Outline the basic methods and logic of Beadle and Tatums experiments. Why are
their results important?
Additional Problems
1. The following growth responses (+ = growth, 0 = no growth) of mutant strains 1-4
were seen on the related biosynthetic intermediates A E (i.e. A E are
hypothesized to be intermediates in a biochemical/metabolic pathway):
Growth on:
strain A B C D E
mutant 1 0 0 0 0 +
mutant 2 + 0 0 0 +
mutant 3 + 0 + + +
mutant 4 + 0 + 0 +
Assume all intermediates are able to enter the cell, that each mutant strain carries only
one mutation and that the pathway is linear (no branches). What is the sequence of the
biosynthetic intermediates in the pathway?
2
2. Three unlinked genes encode three enzymes that catalyze the same reaction in
ultramaroons:
x+ y+ z+
colorless purple
compound compound
a+ x+ y+ z+
colorless colorless purple
compound 1 compound 2 compound
3. Choose the correct answers, noting that each blank may have more than one correct
answer and that each answer (1 through 4) can be used more than once.