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y-intercept 0.1643
slope 0.0475
x-value 3.4589473684
Trial 1 Trial 2
0.239 0.237
Champion Bar 0.231 0.221
0.234 0.229
mean 0.235 0.229
0.176 0.181
Champion Powder 0.169 0.165
0.180 0.182
mean 0.175 0.176
0.154 0.157
Perwoll 0.161 0.151
0.149 0.159
mean 0.155 0.156
urve
0.26 0.215
0.231 0.221
0.231 0.218
0.2406666667 0.218
452.2105263158 1.58486708 31.6973415133
0.45103386 9.0206771188
Trial 3 0.01476937 0.295387412
0.231
0.228
0.238 0.003
0.232 0.232 0.008
0.173
0.179
0.184
0.179 0.177
0.153
0.159
0.154
0.155 0.155
slope 0.048856 0.1544627 b
std dev of slope 0.001205 0.0182733 std dev of intercept
R 0.997571 0.023829 std dev of y
fisher stat 1642.895 4 DOF
regression ss 0.932873 0.0022713 error ss Standard Calib
1.6
t value 2.78 1.4
uncertainty slope 0.003351 f(x) = 0.0488557214x + 0.1544626
1.2
uncertainty b 0.0508 R = 0.9975711871
1
0.8
Absorbance
0.6
0.4
0.2
0
0 5 10
PO4
Champion Bar
Champion Powder
Perwoll
Standard Calibration Curve
488557214x + 0.1544626866
975711871
10 15 20 25 30
PO43- ppm
Phosphate Concentration
Trial 1 Trial 2 Trial 3
32.788 30.47035 31.83367
8.384458 8.793456 9.884117
0.068166 0.477164 0.340832
Standard Calibration Curve
Tube Vol. BSA Stock (ml) Vol. DW (ml) Vol. BR (ml) Total Vol. (ml)
1 0.0 1.5 6.0 7.5
2 0.2 0.8 5.0 6.0
3 0.3 0.7 6.0 6.0
4 0.4 0.6 5.0 6.0
5 0.5 0.5 6.0 6.0
6 0.6 0.4 5.0 6.0
7 0.8 0.2 6.0 6.0
8 1.0 0.0 6.0 6.0
Sample
Concentration (with Uncertainty)
True Value %error
Skim Milk
Soy Milk
Non-fat Milk
Whole Milk
Egg
Unknown Sample
e 3
0
0.000 0.005 0.010 0.015
Conc
f(x) = 55.0234693878x + 0.629170068
R = 0.9703180221
f(x) = 40.1234693878x
40.5632653061x + 0.7913367347
0.7512482993
R = 0.9021173981
0.9175659387
f(x) = 36.7285714286x + 0.4387142857
R = 0.7545721825
Concentration, mg/mL
Determination of protein concentration in milk, egg, a
Sample Trial Vol. Sample (ml) Vol. DW (ml) Vol. BR (ml) Total Vol. (ml) Absorbance
1.0 0.2 0.8 5.00 6.00 0.747
Skim milk 2.0 0.2 0.8 5.00 6.00 0.758
3.0 0.2 0.8 5.00 6.00 0.753
average 0.753
1.0 0.2 0.8 5.00 6.00 0.711
Soy milk 2.0 0.2 0.8 5.00 6.00 0.721
3.0 0.2 0.8 5.00 6.00 0.717
average 0.716
1.0 0.2 0.8 5.00 6.00 0.710
Non-fat milk 2.0 0.2 0.8 5.00 6.00 0.701
3.0 0.2 0.8 5.00 6.00 0.692
average 0.701
1.0 0.2 0.8 5.00 6.00 0.720
Whole milk 2.0 0.2 0.8 5.00 6.00 0.711
3.0 0.2 0.8 5.00 6.00 0.717
average 0.716
1.0 0.1 0.9 5.00 6.00 0.559
Egg 2.0 0.1 0.9 5.00 6.00 0.567
3.0 0.1 0.9 5.00 6.00 0.552
average 0.559
1.0 0.2 0.8 5.00 6.00 1.701
Unknown 2.0 0.2 0.8 5.00 6.00 1.671
3.0 0.2 0.8 5.00 6.00 1.659
average 1.677
centration in milk, egg, and unknown sample
R2 0.9021
Slope 40.123
59.8803124836
-7.7935348802 y-intercept 0.7913
6.2258554944 Equation of the Line A595 = 40.123[Protein] + 0.7913
6.1635470927
0.4152456759 Unknown 1
Protein] + 0.7913