Chemical equation

Diaminonaphthalene Se4+ 4,5-

benzoplazselenol

RFIS

1. Adjusting of pH to 2.02- this is the optimum pH for analytical work

and at this pH there is adequate control of interfering ferric ions

2. Hydroxylamine hydrochloride- stabilizing agent

3. Selenium +4 reacts with diaminonaphthalene to form 4,5-

benzopiazselenol.

strong fluorescence characteristics

Could be extracted directly from the reaction mixture into

hydrocarbon solvents.

light sensitive molecule

4. Standing for 100 mins ensures complete reaction

Determine absorbance of standard solution and test solution. Reagent blank

as blank.

5. Cyclohexane- extracting reagent for 4,5-benzopiazselenol. Shaking

vigorously allows complete transfer to the organic solvent.

6. Centrifugation allows separation of remaining water in the cyclohexane

layer.